Restriction map for
pFN21AE3159
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI AspEI AspI AvaI
AviII AvrII BalI BbeI BbrPI
BbsI BbvCI BcgI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstXI BstZ17I Bsu36I Cac8I CelII
CfoI Cfr10I ClaI CpoI DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT14I EcoT22I FauI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII HphI KasI KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MslI MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 1006 GGTACC G^GTACC
AciI 1095 CCGC CCGC(-3/-1)
AfaI 1007 GTAC GT^AC
AflIII 137 ACATGT A^C[GA][CT]GT
AlwNI 506 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 337 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1006 GGTACC G^GTACC
AvaII 550 GGACC G^G[AT]CC
BamHI 806 GGATCC G^GATCC
BanII 709 GAGCTC G[GA]GC[CT]^C
BciVI 596 GGATAC GTATCC(6/5)
BclI 351 TGATCA T^GATCA
BsaJI 901 CCTGGG C^C[ACGT][ACGT]GG
BseRI 998 GAGGAG GAGGAG(10/8)
BspHI 762 TCATGA T^CATGA
BspLU11I 137 ACATGT A^CATGT
BsrDI 581 GCAATG GCAATG(2/0)
BstEII 70 GGTTACC G^GT[ACGT]ACC
BstPI 70 GGTTACC G^GT[ACGT]ACC
BstYI 806 GGATCC [GA]^GATC[CT]
DraI 958 TTTAAA TTT^AAA
DraII 549 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 76 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 549 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 70 GGTTACC G^GT[ACGT]ACC
FbaI 351 TGATCA T^GATCA
HaeIII 84 GGCC GG^CC
KpnI 1006 GGTACC GGTAC^C
MflI 806 GGATCC [GA]^GATC[CT]
MspA1I 206 CAGCTG C[AC]G^C[GT]G
MwoI 694 GCTTCTGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflMI 75 CCACCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 549 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 206 CAGCTG CAG^CTG
RcaI 762 TCATGA T^CATGA
RsaI 1007 GTAC GT^AC
SacI 709 GAGCTC GAGCT^C
SapI 226 GAAGAGC GCTCTTC(1/4)
SfaNI 861 GATGC GCATC(5/9)
SfcI 732 CTACAG C^T[GA][CT]AG
SmlI 423 CTCAAG C^T[CT][GA]AG
SpeI 403 ACTAGT A^CTAGT
Tsp45I 720 GTCAC ^GT[GC]AC
Van91I 75 CCACCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 806 GGATCC [GA]^GATC[CT]
XmnI 337 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AspHI 709 GAGCTC G[AT]GC[AT]^C
835 GTGCTC
BanI 48 GGTGCC G^G[CT][GA]CC
1006 GGTACC
BbvI 175 GCAGC GCAGC(8/12)
178 GCAGC
BmyI 709 GAGCTC G[AGT]GC[ACT]^C
835 GTGCTC
BpmI 662 CTCCAG CTGGAG(16/14)
698 CTGGAG
BsiHKAI 709 GAGCTC G[AT]GC[AT]^C
835 GTGCTC
BsiYI 75 CCACCCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
587 CCACTTTCAGG
BslI 75 CCACCCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
587 CCACTTTCAGG
BsmAI 559 GAGAC GTCTC(1/5)
923 GAGAC
BsmFI 266 GGGAC GGGAC(10/14)
966 GGGAC
BsmI 428 GAATGC GAATGC(1/-1)
908 GAATGC
Bsp1286I 709 GAGCTC G[AGT]GC[ACT]^C
835 GTGCTC
Cfr13I 84 GGCCC G^G[ACGT]CC
550 GGACC
NspI 137 ACATGT [GA]CATG^[CT]
149 ACATGC
Sau96I 84 GGCCC G^G[ACGT]CC
550 GGACC
TaqI 921 TCGA T^CGA
996 TCGA
TseI 175 GCAGC G^C[AT]GC
178 GCAGC
TthHB8I 921 TCGA T^CGA
996 TCGA
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AcsI AluI AlwI ApoI BfaI
BsrI BssKI BstF5I BstNI DdeI
DpnII EarI EcoRII Fnu4HI FokI
HinfI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MvaI
NdeII NlaIII NlaIV PleI Sau3AI
ScrFI TfiI Tru9I Tsp509I TspRI