(Circular) (Six-base) MAPSORT of: pFN21AE3159.seq Check: 6112 from: 1 to: 5839 pFN21AE3159 5839 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3649 278 Size: 53 83 186 936 2113 2468 Fragments arranged by size: 2468 2113 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3182 1937 Size: 1245 4594 Acc65I G'GTAC_C Cuts at: 3011 3160 3011 Size: 149 5690 AclI AA'CG_TT Cuts at: 1563 3966 4339 1563 Size: 2403 373 3063 Fragments arranged by size: 3063 2403 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2388 2475 3902 5286 5554 1632 Size: 756 87 1427 1384 268 1917 Fragments arranged by size: 1917 1427 1384 756 268 87 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5642 AflIII A'CryG_T Cuts at: 1203 2142 4738 5715 1203 Size: 939 2596 977 1327 Fragments arranged by size: 2596 1327 977 939 AgeI A'CCGG_T Cuts at: 4670 4670 Size: 5839 AhdI GACnn_n'nnGTC Cuts at: 4568 4568 Size: 5839 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5839 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5839 AlwNI CAG_nnn'CTG Cuts at: 2516 3582 5154 2516 Size: 1066 1572 3201 Fragments arranged by size: 3201 1572 1066 ApaLI G'TGCA_C Cuts at: 3896 5052 3896 Size: 1156 4683 ApoI r'AATT_y Cuts at: 1493 2023 2333 2343 2989 3148 3374 3526 5635 Size: 530 310 10 646 159 226 152 2109 Cuts at: 5635 1493 Size: 1697 Fragments arranged by size: 2109 1697 646 530 310 226 159 152 10 AseI AT'TA_AT Cuts at: 160 1032 4393 160 Size: 872 3361 1606 Fragments arranged by size: 3361 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5839 AvaI C'yCGr_G Cuts at: 1870 1943 3164 1870 Size: 73 1221 4545 Fragments arranged by size: 4545 1221 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2056 2089 2056 Size: 33 5806 BamHI G'GATC_C Cuts at: 2811 3169 3536 2811 Size: 358 367 5114 Fragments arranged by size: 5114 367 358 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2053 3011 3160 4615 618 Size: 325 217 627 266 958 149 1455 1842 Fragments arranged by size: 1842 1455 958 627 325 266 217 149 BanII G_rGCy'C Cuts at: 1391 2718 3158 1391 Size: 1327 440 4072 Fragments arranged by size: 4072 1327 440 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4868 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4030 4064 1903 Size: 34 2093 34 3678 Fragments arranged by size: 3678 2093 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2595 3731 4941 2595 Size: 1136 1210 3493 Fragments arranged by size: 3493 1210 1136 BclI T'GATC_A Cuts at: 1548 2356 1548 Size: 808 5031 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4450 136 Size: 107 122 71 631 605 2778 1525 Fragments arranged by size: 2778 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5834 5834 Size: 5839 BlpI GC'TnA_GC Cuts at: 3256 3256 Size: 5839 Bme1580I G_kGCm'C Cuts at: 1790 3900 5056 1790 Size: 2110 1156 2573 Fragments arranged by size: 2573 2110 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5839 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2078 2278 2743 Size: 416 53 83 399 24 661 200 465 Cuts at: 2743 3570 4528 442 Size: 827 958 1753 Fragments arranged by size: 1753 958 827 661 465 416 399 200 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5839 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2652 2724 4499 1239 Size: 1413 72 1775 2579 Fragments arranged by size: 2579 1775 1413 72 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2413 3964 4829 5127 2413 Size: 1551 865 298 3125 Fragments arranged by size: 3125 1551 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4502 882 Size: 617 3003 2219 Fragments arranged by size: 3003 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3630 493 Size: 711 2426 2702 Fragments arranged by size: 2702 2426 711 BsaBI GATnn'nnATC Cuts at: 5833 5833 Size: 5839 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3646 4028 4663 275 Size: 53 83 186 936 2113 382 635 1451 Fragments arranged by size: 2113 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4272 4670 4944 5091 5502 4272 Size: 398 274 147 411 4609 Fragments arranged by size: 4609 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5614 Fragments arranged by size: 5614 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3018 1186 Size: 1832 4007 BseYI C'CCAG_C Cuts at: 2119 5042 5538 2119 Size: 2923 496 2420 Fragments arranged by size: 2923 2420 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5266 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3289 4050 5078 1540 Size: 275 186 1288 761 1028 2301 Fragments arranged by size: 2301 1288 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2718 2844 3900 3985 5056 2718 Size: 126 1056 85 1071 3501 Fragments arranged by size: 3501 1071 1056 126 85 BsmI GAATG_Cn' Cuts at: 2439 2919 3350 3443 2439 Size: 480 431 93 4835 Fragments arranged by size: 4835 480 431 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2718 2844 3158 3900 3985 5056 1391 Size: 399 928 126 314 742 85 1071 2174 Fragments arranged by size: 2174 1071 928 742 399 314 126 85 BspEI T'CCGG_A Cuts at: 5502 5502 Size: 5839 BspHI T'CATG_A Cuts at: 2767 3728 5458 2767 Size: 961 1730 3148 Fragments arranged by size: 3148 1730 961 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3194 844 Size: 2350 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3726 1394 Size: 429 1903 3507 Fragments arranged by size: 3507 1903 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2593 4334 4508 65 Size: 1183 1345 1741 174 1396 Fragments arranged by size: 1741 1396 1345 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4483 4670 1700 Size: 110 2673 187 2869 Fragments arranged by size: 2869 2673 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5839 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5839 BssSI C'ACGA_G Cuts at: 3899 4911 3899 Size: 1012 4827 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5839 BstBI TT'CG_AA Cuts at: 4643 4643 Size: 5839 BstEII G'GTnAC_C Cuts at: 1184 2075 1184 Size: 891 4948 BstYI r'GATC_y Cuts at: 1972 2811 3169 3536 3921 3938 5390 5476 5488 Size: 839 358 367 385 17 1452 86 12 Cuts at: 5488 5834 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 839 385 367 358 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5287 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5100 Fragments arranged by size: 5100 683 56 BtsI GCAGTG_nn' Cuts at: 2165 3355 4149 4169 2165 Size: 1190 794 20 3835 Fragments arranged by size: 3835 1190 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5839 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5804 DraI TTT'AAA Cuts at: 2965 3143 2965 Size: 178 5661 DraIII CAC_nnn'GTG Cuts at: 2086 2086 Size: 5839 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4846 809 Size: 507 3530 1802 Fragments arranged by size: 3530 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3286 4175 5804 Size: 54 1425 50 275 18 1456 889 1629 Cuts at: 5804 8 Size: 43 Fragments arranged by size: 1629 1456 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3286 1537 Size: 275 1474 4090 Fragments arranged by size: 4090 1474 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2226 2364 2851 3769 1353 Size: 873 138 487 918 3423 Fragments arranged by size: 3423 918 873 487 138 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4426 4800 4946 387 Size: 1064 138 2837 374 146 1280 Fragments arranged by size: 2837 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2716 2716 Size: 5839 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2388 2475 2652 2724 3902 4499 5286 Size: 393 756 87 177 72 1178 597 787 Cuts at: 5286 5554 1239 Size: 268 1524 Fragments arranged by size: 1524 1178 787 756 597 393 268 177 87 72 EcoO109I rG'GnC_Cy Cuts at: 2555 2555 Size: 5839 EcoRI G'AATT_C Cuts at: 3148 3526 3148 Size: 378 5461 FspI TGC'GCA Cuts at: 4345 4345 Size: 5839 HaeII r_GCGC'y Cuts at: 1678 4986 1678 Size: 3308 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4480 4512 4554 4586 1373 Size: 32 135 32 2908 32 42 32 2626 Fragments arranged by size: 2908 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3183 3429 669 Size: 988 281 1245 246 3079 Fragments arranged by size: 3079 1245 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5839 HpaI GTT'AAC Cuts at: 3429 3429 Size: 5839 KpnI G_GTAC'C Cuts at: 3015 3164 3015 Size: 149 5690 MfeI C'AATT_G Cuts at: 3438 3438 Size: 5839 MluI A'CGCG_T Cuts at: 5715 5715 Size: 5839 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2550 4953 5137 1305 Size: 1245 2403 184 2007 Fragments arranged by size: 2403 2007 1245 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4017 Fragments arranged by size: 4017 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3797 4156 4315 518 Size: 648 843 1788 359 159 2042 Fragments arranged by size: 2042 1788 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2213 3255 3932 5080 5325 1606 Size: 607 1042 677 1148 245 2120 Fragments arranged by size: 2120 1148 1042 677 607 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5287 NdeI CA'TA_TG Cuts at: 387 387 Size: 5839 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5839 NotI GC'GGCC_GC Cuts at: 3286 3286 Size: 5839 NspI r_CATG'y Cuts at: 1132 1229 2146 2158 3197 4742 1132 Size: 97 917 12 1039 1545 2229 Fragments arranged by size: 2229 1545 1039 917 97 12 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5839 PciI A'CATG_T Cuts at: 2142 4738 2142 Size: 2596 3243 PflMI CCAn_nnn'nTGG Cuts at: 1108 2086 1108 Size: 978 4861 PfoI T'CCnGG_A Cuts at: 2814 2814 Size: 5839 PmeI GTTT'AAAC Cuts at: 3143 3143 Size: 5839 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3908 3940 5421 5453 3908 Size: 32 1481 32 4294 Fragments arranged by size: 4294 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2555 2555 Size: 5839 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5839 PsiI TTA'TAA Cuts at: 3409 3409 Size: 5839 PstI C_TGCA'G Cuts at: 830 3191 830 Size: 2361 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5839 PvuII CAG'CTG Cuts at: 2213 2213 Size: 5839 SacI G_AGCT'C Cuts at: 2718 2718 Size: 5839 SalI G'TCGA_C Cuts at: 1936 3181 1936 Size: 1245 4594 SapI GCTCTTCn'nnn_ Cuts at: 2226 2226 Size: 5839 SbfI CC_TGCA'GG Cuts at: 3191 3191 Size: 5839 ScaI AGT'ACT Cuts at: 4087 5799 4087 Size: 1712 4127 SfcI C'TryA_G Cuts at: 826 1045 2737 3187 4322 5003 5194 826 Size: 219 1692 450 1135 681 191 1471 Fragments arranged by size: 1692 1471 1135 681 450 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5839 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5839 SmaI CCC'GGG Cuts at: 1872 3166 1872 Size: 1294 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2428 3943 4844 5106 820 Size: 197 926 485 1515 901 262 1553 Fragments arranged by size: 1553 1515 926 901 485 262 197 SnaBI TAC'GTA Cuts at: 493 3630 493 Size: 3137 2702 SpeI A'CTAG_T Cuts at: 152 2408 152 Size: 2256 3583 SphI G_CATG'C Cuts at: 1132 3197 1132 Size: 2065 3774 SspI AAT'ATT Cuts at: 5 52 3763 5 Size: 47 3711 2081 Fragments arranged by size: 3711 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5839 StyI C'CwwG_G Cuts at: 513 1065 3278 513 Size: 552 2213 3074 Fragments arranged by size: 3074 2213 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2097 3866 3883 4036 4221 4666 325 Size: 1255 517 1769 17 153 185 445 1498 Fragments arranged by size: 1769 1498 1255 517 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4085 5797 96 Size: 274 80 33 51 1443 2108 1712 138 Fragments arranged by size: 2108 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2619 2651 2857 2889 5799 5831 2619 Size: 32 206 32 2910 32 2627 Fragments arranged by size: 2910 2627 206 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5839 XbaI T'CTAG_A Cuts at: 3175 3175 Size: 5839 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5839 XmaI C'CCGG_G Cuts at: 1870 3164 1870 Size: 1294 4545 XmnI GAAnn'nnTTC Cuts at: 2346 3968 2346 Size: 1622 4217 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3647 276 Size: 53 83 186 936 2113 2468 Fragments arranged by size: 2468 2113 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI