################################## # NO CUT ENZYMES ( 190 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaII AviII AvrII BanI BanII BbeI BbrPI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI KspI MaeII MaeIII MamI MfeI MluI MroI MslI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TaqI Tsp45I Tth111I TthHB8I Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccI 158 GTCTAC GT^[AC][GT]AC AcsI 322 AAATTC [GA]^AATT[CT] AlwNI 480 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 322 AAATTC [GA]^AATT[CT] AvaI 618 CCCGAG C^[CT]CG[GA]G BalI 233 TGGCCA TGG^CCA BamHI 449 GGATCC G^GATCC BbsI 569 GAAGAC GAAGAC(2/6) BbvCI 205 CCTCAGC CCTCAGC(-5/-2) BbvI 36 GCTGC GCAGC(8/12) BglII 260 AGATCT A^GATCT BpuAI 569 GAAGAC GAAGAC(2/6) BsmAI 542 GTCTC GTCTC(1/5) BsmFI 372 GGGAC GGGAC(10/14) BsoBI 618 CCCGAG C^[CT]CG[GA]G BsrI 375 ACTGG ACTGG(1/-1) BstF5I 294 GGATG GGATG(2/0) Cac8I 39 GCTGGC GC[ACGT]^[ACGT]GC Cfr13I 494 GGGCC G^G[ACGT]CC DraI 114 TTTAAA TTT^AAA EaeI 233 TGGCCA [CT]^GGCC[GA] EarI 271 CTCTTC CTCTTC(1/4) Fnu4HI 36 GCTGC GC^[ACGT]GC FokI 294 GGATG GGATG(9/13) ItaI 36 GCTGC GC^[ACGT]GC Ksp632I 271 CTCTTC CTCTTC(1/4) MluNI 233 TGGCCA TGG^CCA MscI 233 TGGCCA TGG^CCA NlaIV 449 GGATCC GG[ACGT]^[ACGT]CC PleI 201 GAGTC GAGTC(4/5) Sau96I 494 GGGCC G^G[ACGT]CC SfaNI 249 GATGC GCATC(5/9) SspI 283 AATATT AAT^ATT TfiI 102 GAATC G^A[AT]TC TseI 36 GCTGC G^C[AT]GC TspRI 154 CAGTG CA[GC]TG[ACGT][ACGT]^ XbaI 63 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AlwI 450 GATCC GGATC(4/5) 449 GGATC BsaJI 491 CCTGGG C^C[ACGT][ACGT]GG 619 CCGAGG BsiYI 270 CCTCTTCAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 485 CCTGTGCCTGG BslI 270 CCTCTTCAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 485 CCTGTGCCTGG BstYI 260 AGATCT [GA]^GATC[CT] 449 GGATCC DpnII 261 GATC ^GATC 450 GATC HinfI 102 GAATC G^A[ACGT]TC 201 GAGTC MboI 261 GATC ^GATC 450 GATC MflI 260 AGATCT [GA]^GATC[CT] 449 GGATCC MseI 115 TTAA T^TAA 431 TTAA NdeII 261 GATC ^GATC 450 GATC Sau3AI 261 GATC ^GATC 450 GATC SfcI 477 CTACAG C^T[GA][CT]AG 602 CTACAG Tru9I 115 TTAA T^TAA 431 TTAA Tsp509I 147 AATT ^AATT 323 AATT XhoII 260 AGATCT [GA]^GATC[CT] 449 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 15 enzymes) # ######################################### AfaI AluI BfaI BssKI BstNI DdeI EcoRII HaeIII MaeI MboII MnlI MvaI NlaIII RsaI ScrFI