Restriction map for
pFN21AE3134
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstPI BstUI Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI HphI
KasI KpnI KspI MaeI MamI
MfeI MluI MroI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tsp45I Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccI 781 GTATAC GT^[AC][GT]AC
ApaI 969 GGGCCC GGGCC^C
Asp700I 296 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1005 GAGCAC G[AT]GC[AT]^C
BalI 921 TGGCCA TGG^CCA
BciVI 773 GGATAC GTATCC(6/5)
BclI 824 TGATCA T^GATCA
BcnI 289 CCGGG CC^[GC]GG
BsiHKAI 1005 GAGCAC G[AT]GC[AT]^C
Bst1107I 781 GTATAC GTA^TAC
BstZ17I 781 GTATAC GTA^TAC
DraI 837 TTTAAA TTT^AAA
DraII 968 GGGGCCC [GA]G^G[ACGT]CC[CT]
DsaI 846 CCACGG C^C[GA][CT]GG
EaeI 921 TGGCCA [CT]^GGCC[GA]
EcoO109I 968 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 803 CCAAGG C^C[AT][AT]GG
FbaI 824 TGATCA T^GATCA
HapII 289 CCGG C^CGG
HindIII 34 AAGCTT A^AGCTT
HpaII 289 CCGG C^CGG
MluNI 921 TGGCCA TGG^CCA
MscI 921 TGGCCA TGG^CCA
MspA1I 10 CAGCTG C[AC]G^C[GT]G
MspI 289 CCGG C^CGG
NciI 289 CCGGG CC^[GC]GG
NspI 790 ACATGC [GA]CATG^[CT]
PleI 673 GACTC GAGTC(4/5)
PstI 448 CTGCAG CTGCA^G
PvuII 10 CAGCTG CAG^CTG
SspI 335 AATATT AAT^ATT
StuI 755 AGGCCT AGG^CCT
StyI 803 CCAAGG C^C[AT][AT]GG
TaqI 651 TCGA T^CGA
TthHB8I 651 TCGA T^CGA
XmnI 296 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcsI 711 GAATTT [GA]^AATT[CT]
833 AAATTT
ApoI 711 GAATTT [GA]^AATT[CT]
833 AAATTT
BanII 934 GGGCTC G[GA]GC[CT]^C
969 GGGCCC
BpmI 425 CTGGAG CTGGAG(16/14)
767 CTGGAG
BsiYI 366 CCCAGTGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
949 CCAGATAAGGG
BslI 366 CCCAGTGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
949 CCAGATAAGGG
BstXI 139 CCATCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
924 CCAGGAATGTGG
BstYI 238 GGATCT [GA]^GATC[CT]
408 GGATCT
EarI 359 CTCTTC CTCTTC(1/4)
719 CTCTTC
Ksp632I 359 CTCTTC CTCTTC(1/4)
719 CTCTTC
MaeII 520 ACGT A^CGT
762 ACGT
MaeIII 272 GTTAC ^GT[ACGT]AC
851 GTAAC
MflI 238 GGATCT [GA]^GATC[CT]
408 GGATCT
MslI 475 CATTGTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
858 CATGCCTATG
MwoI 3 GCTGACCCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
748 GCAATTAAGGC
NlaIV 968 GGGGCC GG[ACGT]^[ACGT]CC
969 GGGCCC
XhoII 238 GGATCT [GA]^GATC[CT]
408 GGATCT
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# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AluI AlwI BbvI BmyI BsaJI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HinfI ItaI
MboI MboII MnlI MseI MvaI
NdeII NlaIII Sau3AI Sau96I ScrFI
SfaNI SfcI TfiI Tru9I TseI
Tsp509I TspRI