(Circular) (Six-base) MAPSORT of: pFN21AE3134.seq Check: 4780 from: 1 to: 5725 pFN21AE3134 5725 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3535 278 Size: 53 83 186 936 1999 2468 Fragments arranged by size: 2468 1999 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2787 3068 1937 Size: 850 281 4594 Fragments arranged by size: 4594 850 281 Acc65I G'GTAC_C Cuts at: 3046 3046 Size: 5725 AclI AA'CG_TT Cuts at: 1563 3852 4225 1563 Size: 2289 373 3063 Fragments arranged by size: 3063 2289 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2292 3788 5172 5440 1632 Size: 660 1496 1384 268 1917 Fragments arranged by size: 1917 1496 1384 660 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5528 AflIII A'CryG_T Cuts at: 1203 4624 5601 1203 Size: 3421 977 1327 Fragments arranged by size: 3421 1327 977 AgeI A'CCGG_T Cuts at: 4556 4556 Size: 5725 AhdI GACnn_n'nnGTC Cuts at: 4454 4454 Size: 5725 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5725 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2911 1184 Size: 1727 3998 AlwNI CAG_nnn'CTG Cuts at: 3468 5040 3468 Size: 1572 4153 ApaI G_GGCC'C Cuts at: 2978 2978 Size: 5725 ApaLI G'TGCA_C Cuts at: 3782 4938 3782 Size: 1156 4569 ApoI r'AATT_y Cuts at: 1493 2716 2838 3034 3260 3412 5521 1493 Size: 1223 122 196 226 152 2109 1697 Fragments arranged by size: 2109 1697 1223 226 196 152 122 AseI AT'TA_AT Cuts at: 160 1032 4279 160 Size: 872 3247 1606 Fragments arranged by size: 3247 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5725 AvaI C'yCGr_G Cuts at: 1870 1943 3050 1870 Size: 73 1107 4545 Fragments arranged by size: 4545 1107 73 BamHI G'GATC_C Cuts at: 3055 3422 3055 Size: 367 5358 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3046 4501 618 Size: 325 217 627 1259 1455 1842 Fragments arranged by size: 1842 1455 1259 627 325 217 BanII G_rGCy'C Cuts at: 1391 2943 2978 3044 1391 Size: 1552 35 66 4072 Fragments arranged by size: 4072 1552 66 35 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4754 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3916 3950 1903 Size: 34 50 34 1895 34 3678 Fragments arranged by size: 3678 1895 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2772 3617 4827 2772 Size: 845 1210 3670 Fragments arranged by size: 3670 1210 845 BclI T'GATC_A Cuts at: 1548 2829 1548 Size: 1281 4444 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4336 136 Size: 107 122 71 631 605 2664 1525 Fragments arranged by size: 2664 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5720 5720 Size: 5725 BlpI GC'TnA_GC Cuts at: 3142 3142 Size: 5725 Bme1580I G_kGCm'C Cuts at: 1790 2978 3786 4942 1790 Size: 1188 808 1156 2573 Fragments arranged by size: 2573 1188 1156 808 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5725 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2366 3456 4414 Size: 416 53 83 399 24 949 1090 958 Cuts at: 4414 442 Size: 1753 Fragments arranged by size: 1753 1090 958 949 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5725 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2451 2793 4385 1239 Size: 1212 342 1592 2579 Fragments arranged by size: 2579 1592 1212 342 Bpu10I CC'TnA_GC Cuts at: 2282 2516 2282 Size: 234 5491 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3850 4715 5013 3850 Size: 865 298 4562 Fragments arranged by size: 4562 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4388 882 Size: 617 2889 2219 Fragments arranged by size: 2889 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3516 493 Size: 711 2312 2702 Fragments arranged by size: 2702 2312 711 BsaBI GATnn'nnATC Cuts at: 5719 5719 Size: 5725 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3532 3914 4549 275 Size: 53 83 186 936 1999 382 635 1451 Fragments arranged by size: 1999 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4158 4556 4830 4977 5388 4158 Size: 398 274 147 411 4495 Fragments arranged by size: 4495 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2902 2932 1178 Size: 30 165 30 1499 30 3971 Fragments arranged by size: 3971 1499 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5725 BseYI C'CCAG_C Cuts at: 2013 2604 4928 5424 2013 Size: 591 2324 496 2314 Fragments arranged by size: 2324 2314 591 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5152 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3175 3936 4964 1540 Size: 275 186 1174 761 1028 2301 Fragments arranged by size: 2301 1174 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3014 3786 3871 4942 3014 Size: 772 85 1071 3797 Fragments arranged by size: 3797 1071 772 85 BsmI GAATG_Cn' Cuts at: 3236 3329 3236 Size: 93 5632 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2943 2978 3014 3044 3786 3871 4942 Size: 399 1153 35 36 30 742 85 1071 Cuts at: 4942 1391 Size: 2174 Fragments arranged by size: 2174 1153 1071 742 399 85 36 35 30 BspEI T'CCGG_A Cuts at: 5388 5388 Size: 5725 BspHI T'CATG_A Cuts at: 3614 5344 3614 Size: 1730 3995 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3080 844 Size: 2236 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3612 1394 Size: 429 1789 3507 Fragments arranged by size: 3507 1789 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4220 4394 65 Size: 1183 2972 174 1396 Fragments arranged by size: 2972 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4369 4556 1700 Size: 110 2559 187 2869 Fragments arranged by size: 2869 2559 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5725 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5725 BssSI C'ACGA_G Cuts at: 3785 4797 3785 Size: 1012 4713 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5725 BstBI TT'CG_AA Cuts at: 4529 4529 Size: 5725 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5725 BstXI CCAn_nnnn'nTGG Cuts at: 2151 2936 2151 Size: 785 4940 BstYI r'GATC_y Cuts at: 1972 2243 2413 3055 3422 3807 3824 5276 5362 Size: 271 170 642 367 385 17 1452 86 Cuts at: 5362 5374 5720 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 642 385 367 346 271 170 86 17 12 BstZ17I GTA'TAC Cuts at: 2788 2788 Size: 5725 BtgI C'CryG_G Cuts at: 513 1065 2851 513 Size: 552 1786 3387 Fragments arranged by size: 3387 1786 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4986 Fragments arranged by size: 4986 683 56 BtsI GCAGTG_nn' Cuts at: 2462 3241 4035 4055 2462 Size: 779 794 20 4132 Fragments arranged by size: 4132 794 779 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5725 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2532 2567 529 Size: 35 1968 35 3687 Fragments arranged by size: 3687 1968 35 35 DraI TTT'AAA Cuts at: 2844 3029 2844 Size: 185 5540 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4732 809 Size: 507 3416 1802 Fragments arranged by size: 3416 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2926 3172 4061 Size: 54 1425 50 275 18 1096 246 889 Cuts at: 4061 5690 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1096 889 275 246 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3172 1537 Size: 275 1360 4090 Fragments arranged by size: 4090 1360 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2370 2730 3655 1353 Size: 1017 360 925 3423 Fragments arranged by size: 3423 1017 925 360 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4312 4686 4832 387 Size: 1064 138 2723 374 146 1280 Fragments arranged by size: 2723 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2292 2451 2793 3788 4385 5172 5440 Size: 393 660 159 342 995 597 787 268 Cuts at: 5440 1239 Size: 1524 Fragments arranged by size: 1524 995 787 660 597 393 342 268 159 EcoO109I rG'GnC_Cy Cuts at: 2974 2974 Size: 5725 EcoRI G'AATT_C Cuts at: 3034 3412 3034 Size: 378 5347 FspI TGC'GCA Cuts at: 4231 4231 Size: 5725 HaeII r_GCGC'y Cuts at: 1678 4872 1678 Size: 3194 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4366 4398 4440 4472 1373 Size: 32 135 32 2794 32 42 32 2626 Fragments arranged by size: 2794 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3069 3315 669 Size: 988 281 1131 246 3079 Fragments arranged by size: 3079 1131 988 281 246 HindIII A'AGCT_T Cuts at: 748 2039 748 Size: 1291 4434 HpaI GTT'AAC Cuts at: 3315 3315 Size: 5725 KpnI G_GTAC'C Cuts at: 3050 3050 Size: 5725 MfeI C'AATT_G Cuts at: 3324 3324 Size: 5725 MluI A'CGCG_T Cuts at: 5601 5601 Size: 5725 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2049 2508 4839 5023 1305 Size: 744 459 2331 184 2007 Fragments arranged by size: 2331 2007 744 459 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2928 10 Size: 54 1425 343 1096 2807 Fragments arranged by size: 2807 1425 1096 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2484 2867 3683 4042 4201 518 Size: 648 1318 383 816 359 159 2042 Fragments arranged by size: 2042 1318 816 648 383 359 159 MspA1I CmG'CkG Cuts at: 1606 2017 3141 3818 4966 5211 1606 Size: 411 1124 677 1148 245 2120 Fragments arranged by size: 2120 1148 1124 677 411 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5173 NdeI CA'TA_TG Cuts at: 387 387 Size: 5725 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5725 NotI GC'GGCC_GC Cuts at: 3172 3172 Size: 5725 NspI r_CATG'y Cuts at: 1132 1229 2799 3083 4628 1132 Size: 97 1570 284 1545 2229 Fragments arranged by size: 2229 1570 1545 284 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5725 PciI A'CATG_T Cuts at: 4624 4624 Size: 5725 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5725 PfoI T'CCnGG_A Cuts at: 2428 2770 2428 Size: 342 5383 PmeI GTTT'AAAC Cuts at: 3029 3029 Size: 5725 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3794 3826 5307 5339 3794 Size: 32 1481 32 4180 Fragments arranged by size: 4180 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5725 PsiI TTA'TAA Cuts at: 3295 3295 Size: 5725 PspOMI G'GGCC_C Cuts at: 2974 2974 Size: 5725 PstI C_TGCA'G Cuts at: 830 2457 3077 830 Size: 1627 620 3478 Fragments arranged by size: 3478 1627 620 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5725 PvuII CAG'CTG Cuts at: 2017 2017 Size: 5725 SalI G'TCGA_C Cuts at: 1936 3067 1936 Size: 1131 4594 SbfI CC_TGCA'GG Cuts at: 3077 3077 Size: 5725 ScaI AGT'ACT Cuts at: 3973 5685 3973 Size: 1712 4013 SfcI C'TryA_G Cuts at: 826 1045 2453 2695 2729 3073 4208 4889 5080 Size: 219 1408 242 34 344 1135 681 191 Cuts at: 5080 826 Size: 1471 Fragments arranged by size: 1471 1408 1135 681 344 242 219 191 34 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5725 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5725 SmaI CCC'GGG Cuts at: 1872 3052 1872 Size: 1180 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3829 4730 4992 820 Size: 197 926 1886 901 262 1553 Fragments arranged by size: 1886 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3516 493 Size: 3023 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5725 SphI G_CATG'C Cuts at: 1132 3083 1132 Size: 1951 3774 SspI AAT'ATT Cuts at: 5 52 2342 3649 5 Size: 47 2290 1307 2081 Fragments arranged by size: 2290 2081 1307 47 StuI AGG'CCT Cuts at: 1517 2762 1517 Size: 1245 4480 StyI C'CwwG_G Cuts at: 513 1065 2808 3164 513 Size: 552 1743 356 3074 Fragments arranged by size: 3074 1743 552 356 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3752 3769 3922 4107 4552 325 Size: 1255 2172 17 153 185 445 1498 Fragments arranged by size: 2172 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3971 5683 96 Size: 274 80 33 51 1443 1994 1712 138 Fragments arranged by size: 1994 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2627 2659 2902 2934 5685 5717 2627 Size: 32 243 32 2751 32 2635 Fragments arranged by size: 2751 2635 243 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5725 XbaI T'CTAG_A Cuts at: 3061 3061 Size: 5725 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5725 XmaI C'CCGG_G Cuts at: 1870 3050 1870 Size: 1180 4545 XmnI GAAnn'nnTTC Cuts at: 2305 3854 2305 Size: 1549 4176 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3533 276 Size: 53 83 186 936 1999 2468 Fragments arranged by size: 2468 1999 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI