Restriction map for
pFN21AE3124
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BbeI BbrPI BbsI BbvCI
BcgI BciVI BfrI BglI BglII
BlnI BpuAI BsaHI BsaWI BseAI
BsgI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrFI BsrGI BsrI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI Hin1I HincII HindII
HindIII HinfI HpaI KasI KpnI
KspI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NcoI
NdeI NgoMIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tsp45I
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AccII 886 CGCG CG^CG
ApaI 923 GGGCCC GGGCC^C
AvaI 295 CCCGAG C^[CT]CG[GA]G
BclI 425 TGATCA T^GATCA
BlpI 32 GCTCAGC GC^T[ACGT]AGC
BpmI 814 CTGGAG CTGGAG(16/14)
Bpu1102I 32 GCTCAGC GC^T[ACGT]AGC
BsaAI 97 TACGTG [CT]AC^GT[GA]
BsaBI 897 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 754 GAGACC GGTCTC(1/5)
BsmBI 275 GAGACG CGTCTC(1/5)
BsoBI 295 CCCGAG C^[CT]CG[GA]G
BssSI 78 CACGAG CACGAG(-5/-1)
BstUI 886 CGCG CG^CG
CelII 32 GCTCAGC GC^T[ACGT]AGC
EaeI 789 TGGCCG [CT]^GGCC[GA]
EcoNI 852 CCTGGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 425 TGATCA T^GATCA
HgaI 144 GACGC GACGC(5/10)
MaeII 98 ACGT A^CGT
MaeIII 503 GTAAC ^GT[ACGT]AC
MamI 897 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 587 TTAA T^TAA
MslI 192 CATGGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 886 CGCG CG^CG
NheI 324 GCTAGC G^CTAGC
SacI 30 GAGCTC GAGCT^C
ScaI 338 AGTACT AGT^ACT
StuI 354 AGGCCT AGG^CCT
Tru9I 587 TTAA T^TAA
Tsp509I 529 AATT ^AATT
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# TWO CUT ENZYMES ( 19 enzymes) #
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AlwNI 44 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
142 CAGACGCTG
AspHI 30 GAGCTC G[AT]GC[AT]^C
199 GTGCTC
BamHI 221 GGATCC G^GATCC
896 GGATCC
BanI 39 GGTGCC G^G[CT][GA]CC
285 GGTGCC
BfaI 325 CTAG C^TAG
346 CTAG
BsiHKAI 30 GAGCTC G[AT]GC[AT]^C
199 GTGCTC
BsmFI 795 GGGAC GGGAC(10/14)
418 GTCCC
BsrBI 868 GAGCGG CCGCTC(-3/-3)
874 GAGCGG
BstYI 221 GGATCC [GA]^GATC[CT]
896 GGATCC
DdeI 33 CTCAG C^T[ACGT]AG
704 CTGAG
EarI 4 GAAGAG CTCTTC(1/4)
364 GAAGAG
HaeII 839 GGCGCT [GA]GCGC^[CT]
908 AGCGCC
Ksp632I 4 GAAGAG CTCTTC(1/4)
364 GAAGAG
MaeI 325 CTAG C^TAG
346 CTAG
MflI 221 GGATCC [GA]^GATC[CT]
896 GGATCC
PflMI 351 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
846 CCACCACCTGG
TspRI 210 CAGTG CA[GC]TG[ACGT][ACGT]^
702 CACTG
Van91I 351 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
846 CCACCACCTGG
XhoII 221 GGATCC [GA]^GATC[CT]
896 GGATCC
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# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanII BbvI BcnI BmyI BsaJI
BseRI BsiYI BslI BsmAI Bsp1286I
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DpnII DraII EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MboI MboII MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PpuMI RsaI Sau3AI
Sau96I ScrFI StyI TaqI TseI
TthHB8I