(Circular) (Six-base) MAPSORT of: pFN21AE3124.seq Check: 3261 from: 1 to: 5644 pFN21AE3124 5644 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3454 278 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2987 1937 Size: 1050 4594 Acc65I G'GTAC_C Cuts at: 2965 2965 Size: 5644 AclI AA'CG_TT Cuts at: 1563 3771 4144 1563 Size: 2208 373 3063 Fragments arranged by size: 3063 2208 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2195 2697 3707 5091 5359 1632 Size: 563 502 1010 1384 268 1917 Fragments arranged by size: 1917 1384 1010 563 502 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5447 AflIII A'CryG_T Cuts at: 1203 4543 5520 1203 Size: 3340 977 1327 Fragments arranged by size: 3340 1327 977 AgeI A'CCGG_T Cuts at: 4475 4475 Size: 5644 AhdI GACnn_n'nnGTC Cuts at: 4373 4373 Size: 5644 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5644 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2219 1184 Size: 1035 4609 AlwNI CAG_nnn'CTG Cuts at: 2054 2152 3387 4959 2054 Size: 98 1235 1572 2739 Fragments arranged by size: 2739 1572 1235 98 ApaI G_GGCC'C Cuts at: 2932 2932 Size: 5644 ApaLI G'TGCA_C Cuts at: 3701 4857 3701 Size: 1156 4488 ApoI r'AATT_y Cuts at: 1493 2953 3179 3331 5440 1493 Size: 1460 226 152 2109 1697 Fragments arranged by size: 2109 1697 1460 226 152 AseI AT'TA_AT Cuts at: 160 1032 4198 160 Size: 872 3166 1606 Fragments arranged by size: 3166 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 AvaI C'yCGr_G Cuts at: 1870 1943 2300 2969 1870 Size: 73 357 669 4545 Fragments arranged by size: 4545 669 357 73 BamHI G'GATC_C Cuts at: 2226 2901 2974 3341 2226 Size: 675 73 367 4529 Fragments arranged by size: 4529 675 367 73 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2044 2290 2965 4420 618 Size: 325 217 627 257 246 675 1455 1842 Fragments arranged by size: 1842 1455 675 627 325 257 246 217 BanII G_rGCy'C Cuts at: 1391 2039 2090 2128 2932 2963 1391 Size: 648 51 38 804 31 4072 Fragments arranged by size: 4072 804 648 51 38 31 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4673 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3835 3869 1903 Size: 34 1898 34 3678 Fragments arranged by size: 3678 1898 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3536 4746 3536 Size: 1210 4434 BclI T'GATC_A Cuts at: 1548 2430 1548 Size: 882 4762 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4255 136 Size: 107 122 71 631 605 2583 1525 Fragments arranged by size: 2583 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5639 5639 Size: 5644 BlpI GC'TnA_GC Cuts at: 2038 3061 2038 Size: 1023 4621 Bme1580I G_kGCm'C Cuts at: 1790 2084 2932 3705 4861 1790 Size: 294 848 773 1156 2573 Fragments arranged by size: 2573 1156 848 773 294 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5644 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3375 4333 442 Size: 416 53 83 399 24 1958 958 1753 Fragments arranged by size: 1958 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2333 1056 Size: 1277 4367 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2840 4304 1239 Size: 1601 1464 2579 Fragments arranged by size: 2579 1601 1464 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3769 4634 4932 3769 Size: 865 298 4481 Fragments arranged by size: 4481 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2753 4307 882 Size: 617 1254 1554 2219 Fragments arranged by size: 2219 1554 1254 617 BsaAI yAC'GTr Cuts at: 493 1204 2104 3435 493 Size: 711 900 1331 2702 Fragments arranged by size: 2702 1331 900 711 BsaBI GATnn'nnATC Cuts at: 2906 5638 2906 Size: 2732 2912 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3451 3833 4468 275 Size: 53 83 186 936 1918 382 635 1451 Fragments arranged by size: 1918 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4077 4475 4749 4896 5307 4077 Size: 398 274 147 411 4414 Fragments arranged by size: 4414 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2013 2043 1178 Size: 30 165 30 610 30 4779 Fragments arranged by size: 4779 610 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2336 2771 2885 1186 Size: 1150 435 114 3945 Fragments arranged by size: 3945 1150 435 114 BseYI C'CCAG_C Cuts at: 2090 4847 5343 2090 Size: 2757 496 2391 Fragments arranged by size: 2757 2391 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5071 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3094 3855 4883 1540 Size: 275 186 1093 761 1028 2301 Fragments arranged by size: 2301 1093 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2039 2208 3705 3790 4861 2039 Size: 169 1497 85 1071 2822 Fragments arranged by size: 2822 1497 1071 169 85 BsmI GAATG_Cn' Cuts at: 3155 3248 3155 Size: 93 5551 BsmBI CGTCTCn'nnnn_ Cuts at: 2274 2274 Size: 5644 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2039 2084 2090 2128 2208 2932 2963 Size: 399 249 45 6 38 80 724 31 Cuts at: 2963 3705 3790 4861 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 724 399 249 85 80 45 38 31 6 BspEI T'CCGG_A Cuts at: 5307 5307 Size: 5644 BspHI T'CATG_A Cuts at: 3533 5263 3533 Size: 1730 3914 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2999 844 Size: 2155 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2875 2881 3531 1394 Size: 429 1052 6 650 3507 Fragments arranged by size: 3507 1052 650 429 6 BsrDI GCAATG_nn' Cuts at: 65 1248 4139 4313 65 Size: 1183 2891 174 1396 Fragments arranged by size: 2891 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4288 4475 1700 Size: 110 2478 187 2869 Fragments arranged by size: 2869 2478 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5644 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5644 BssSI C'ACGA_G Cuts at: 2083 3704 4716 2083 Size: 1621 1012 3011 Fragments arranged by size: 3011 1621 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5644 BstBI TT'CG_AA Cuts at: 4448 4448 Size: 5644 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5644 BstYI r'GATC_y Cuts at: 1972 2226 2901 2974 3341 3726 3743 5195 5281 Size: 254 675 73 367 385 17 1452 86 Cuts at: 5281 5293 5639 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 675 385 367 346 254 86 73 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4153 Fragments arranged by size: 4153 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4905 Fragments arranged by size: 4905 683 56 BtsI GCAGTG_nn' Cuts at: 3160 3954 3974 3160 Size: 794 20 4830 Fragments arranged by size: 4830 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5644 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5609 DraI TTT'AAA Cuts at: 2948 2948 Size: 5644 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4651 809 Size: 507 3335 1802 Fragments arranged by size: 3335 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2794 3091 3980 Size: 54 1425 50 275 18 964 297 889 Cuts at: 3980 5609 8 Size: 1629 43 Fragments arranged by size: 1629 1425 964 889 297 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3091 1537 Size: 275 1279 4090 Fragments arranged by size: 4090 1279 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2004 2364 3574 1353 Size: 651 360 1210 3423 Fragments arranged by size: 3423 1210 651 360 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4231 4605 4751 387 Size: 1064 138 2642 374 146 1280 Fragments arranged by size: 2642 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2037 2037 Size: 5644 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2195 2697 2840 3707 4304 5091 5359 Size: 393 563 502 143 867 597 787 268 Cuts at: 5359 1239 Size: 1524 Fragments arranged by size: 1524 867 787 597 563 502 393 268 143 EcoNI CCTnn'n_nnAGG Cuts at: 2861 2939 2861 Size: 78 5566 EcoO109I rG'GnC_Cy Cuts at: 2422 2602 2801 2422 Size: 180 199 5265 Fragments arranged by size: 5265 199 180 EcoRI G'AATT_C Cuts at: 2953 3331 2953 Size: 378 5266 FspI TGC'GCA Cuts at: 4150 4150 Size: 5644 HaeII r_GCGC'y Cuts at: 1678 2848 2917 4791 1678 Size: 1170 69 1874 2531 Fragments arranged by size: 2531 1874 1170 69 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2564 2596 4285 4317 4359 Size: 32 135 32 992 32 1689 32 42 Cuts at: 4359 4391 1373 Size: 32 2626 Fragments arranged by size: 2626 1689 992 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2988 3234 669 Size: 988 281 1050 246 3079 Fragments arranged by size: 3079 1050 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5644 HpaI GTT'AAC Cuts at: 3234 3234 Size: 5644 KpnI G_GTAC'C Cuts at: 2969 2969 Size: 5644 MfeI C'AATT_G Cuts at: 3243 3243 Size: 5644 MluI A'CGCG_T Cuts at: 5520 5520 Size: 5644 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4758 4942 1305 Size: 3453 184 2007 Fragments arranged by size: 3453 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3822 Fragments arranged by size: 3822 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2201 3602 3961 4120 518 Size: 648 1035 1401 359 159 2042 Fragments arranged by size: 2042 1401 1035 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2042 2051 2190 2241 3060 3737 4885 5130 Size: 436 9 139 51 819 677 1148 245 Cuts at: 5130 1606 Size: 2120 Fragments arranged by size: 2120 1148 819 677 436 245 139 51 9 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4153 Fragments arranged by size: 4153 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5644 NheI G'CTAG_C Cuts at: 1052 2329 1052 Size: 1277 4367 NotI GC'GGCC_GC Cuts at: 3091 3091 Size: 5644 NspI r_CATG'y Cuts at: 1132 1229 3002 4547 1132 Size: 97 1773 1545 2229 Fragments arranged by size: 2229 1773 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5644 PciI A'CATG_T Cuts at: 4543 4543 Size: 5644 PflMI CCAn_nnn'nTGG Cuts at: 1108 2362 2857 1108 Size: 1254 495 3895 Fragments arranged by size: 3895 1254 495 PfoI T'CCnGG_A Cuts at: 2817 2817 Size: 5644 PmeI GTTT'AAAC Cuts at: 2948 2948 Size: 5644 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3713 3745 5226 5258 3713 Size: 32 1481 32 4099 Fragments arranged by size: 4099 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2422 2602 2801 2422 Size: 180 199 5265 Fragments arranged by size: 5265 199 180 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5644 PsiI TTA'TAA Cuts at: 3214 3214 Size: 5644 PspOMI G'GGCC_C Cuts at: 2928 2928 Size: 5644 PstI C_TGCA'G Cuts at: 830 2996 830 Size: 2166 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5644 SacI G_AGCT'C Cuts at: 2039 2039 Size: 5644 SalI G'TCGA_C Cuts at: 1936 2986 1936 Size: 1050 4594 SbfI CC_TGCA'GG Cuts at: 2996 2996 Size: 5644 ScaI AGT'ACT Cuts at: 2345 3892 5604 2345 Size: 1547 1712 2385 Fragments arranged by size: 2385 1712 1547 SexAI A'CCwGG_T Cuts at: 2940 2940 Size: 5644 SfcI C'TryA_G Cuts at: 826 1045 2992 4127 4808 4999 826 Size: 219 1947 1135 681 191 1471 Fragments arranged by size: 1947 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5644 SmaI CCC'GGG Cuts at: 1872 2971 1872 Size: 1099 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3748 4649 4911 820 Size: 197 926 1805 901 262 1553 Fragments arranged by size: 1805 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3435 493 Size: 2942 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5644 SphI G_CATG'C Cuts at: 1132 3002 1132 Size: 1870 3774 SspI AAT'ATT Cuts at: 5 52 3568 5 Size: 47 3516 2081 Fragments arranged by size: 3516 2081 47 StuI AGG'CCT Cuts at: 1517 2361 1517 Size: 844 4800 StyI C'CwwG_G Cuts at: 513 1065 2004 2356 2442 2724 3083 513 Size: 552 939 352 86 282 359 3074 Fragments arranged by size: 3074 939 552 359 352 282 86 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2108 3671 3688 3841 4026 4471 325 Size: 1255 528 1563 17 153 185 445 1498 Fragments arranged by size: 1563 1498 1255 528 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2343 3890 5602 Size: 274 80 33 51 1443 366 1547 1712 Cuts at: 5602 96 Size: 138 Fragments arranged by size: 1712 1547 1443 366 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2013 2045 5604 5636 2013 Size: 32 3559 32 2021 Fragments arranged by size: 3559 2021 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5644 XbaI T'CTAG_A Cuts at: 2980 2980 Size: 5644 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5644 XmaI C'CCGG_G Cuts at: 1870 2969 1870 Size: 1099 4545 XmnI GAAnn'nnTTC Cuts at: 3773 3773 Size: 5644 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3452 276 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI