################################## # NO CUT ENZYMES ( 140 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbsI BbvCI BciVI BclI BfaI BfrI BglII BlnI BpuAI BsaBI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstF5I BstPI BstUI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FokI FseI FspI HgaI HincII HindII HindIII HinfI HpaI KpnI KspI MaeI MamI MfeI MluI MluNI MscI MunI MvnI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmeI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacII SalI ScaI SexAI SfuI SmaI SmlI SnaBI SpeI SspBI SspI StyI SwaI TfiI Tsp509I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccIII 118 TCCGGA T^CCGGA AcyI 784 GGCGCC G[GA]^CG[CT]C AlwI 128 GGATC GGATC(4/5) AlwNI 592 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 204 GGGCCC GGGCC^C BanI 784 GGCGCC G^G[CT][GA]CC BbeI 784 GGCGCC GGCGC^C BcgI 193 GCAGGCATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 222 GCTGAGC GC^T[ACGT]AGC Bpu1102I 222 GCTGAGC GC^T[ACGT]AGC BsaHI 784 GGCGCC G[GA]^CG[CT]C BsaWI 118 TCCGGA [AT]^CCGG[AT] BseAI 118 TCCGGA T^CCGGA BsmFI 306 GTCCC GGGAC(10/14) BspEI 118 TCCGGA T^CCGGA BspMI 506 ACCTGC ACCTGC(4/8) BsrBI 225 GAGCGG CCGCTC(-3/-3) BsrI 87 CCAGT ACTGG(1/-1) BstYI 128 GGATCT [GA]^GATC[CT] CelII 222 GCTGAGC GC^T[ACGT]AGC DraII 204 GGGCCCC [GA]G^G[ACGT]CC[CT] EcoNI 717 CCTGGACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 204 GGGCCCC [GA]G^G[ACGT]CC[CT] FauI 227 GCGGG CCCGC(4/6) Hin1I 784 GGCGCC G[GA]^CG[CT]C KasI 784 GGCGCC G^GCGCC MflI 128 GGATCT [GA]^GATC[CT] MroI 118 TCCGGA T^CCGGA MseI 707 TTAA T^TAA NarI 784 GGCGCC GG^CGCC PstI 508 CTGCAG CTGCA^G SacI 167 GAGCTC GAGCT^C SapI 246 GAAGAGC GCTCTTC(1/4) SbfI 507 CCTGCAGG CCTGCA^GG SfcI 508 CTGCAG C^T[GA][CT]AG SfiI 465 GGCCGTGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 784 GGCGCC GGC^GCC SgrAI 135 CGCCGGCG C[GA]^CCGG[CT]G SphI 414 GCATGC GCATG^C Sse8387I 507 CCTGCAGG CCTGCA^GG TaqI 410 TCGA T^CGA Tru9I 707 TTAA T^TAA Tsp45I 386 GTGAC ^GT[GC]AC TthHB8I 410 TCGA T^CGA XhoII 128 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AvaI 324 CCCGAG C^[CT]CG[GA]G 426 CTCGGG BbrPI 183 CACGTG CAC^GTG 580 CACGTG BcnI 331 CCCGG CC^[GC]GG 682 CCCGG BglI 188 GCCTGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 466 GCCGTGCAGGC BpmI 271 CTCCAG CTGGAG(16/14) 586 CTGGAG BsaAI 183 CACGTG [CT]AC^GT[GA] 580 CACGTG BsoBI 324 CCCGAG C^[CT]CG[GA]G 426 CTCGGG BssSI 315 CACGAG CACGAG(-5/-1) 444 CACGAG BstXI 182 CCACGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 209 CCAGGCCTGTGG DdeI 223 CTGAG C^T[ACGT]AG 755 CTGAG DpnII 129 GATC ^GATC 538 GATC EarI 246 GAAGAG CTCTTC(1/4) 261 GAAGAG HaeII 747 GGCGCT [GA]GCGC^[CT] 784 GGCGCC HphI 375 GGTGA GGTGA(8/7) 633 GGTGA Ksp632I 246 GAAGAG CTCTTC(1/4) 261 GAAGAG MaeIII 364 GTAAC ^GT[ACGT]AC 386 GTGAC MboI 129 GATC ^GATC 538 GATC MboII 246 GAAGA GAAGA(8/7) 261 GAAGA MslI 486 CATCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 555 CATCTCTGTG MspA1I 282 CAGCGG C[AC]G^C[GT]G 592 CAGCGG NaeI 136 GCCGGC GCC^GGC 397 GCCGGC NciI 331 CCCGG CC^[GC]GG 682 CCCGG NdeII 129 GATC ^GATC 538 GATC NgoMIV 136 GCCGGC G^CCGGC 397 GCCGGC NspI 197 GCATGT [GA]CATG^[CT] 414 GCATGC PmaCI 183 CACGTG CAC^GTG 580 CACGTG PmlI 183 CACGTG CAC^GTG 580 CACGTG Sau3AI 129 GATC ^GATC 538 GATC StuI 211 AGGCCT AGG^CCT 725 AGGCCT TspRI 575 CACTG CA[GC]TG[ACGT][ACGT]^ 612 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AciI AluI AspHI AvaII BanII BbvI BmyI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrFI BssKI BstAPI BstNI Cac8I CfoI Cfr10I Cfr13I EcoRII Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MaeII MnlI MspI MvaI MwoI NlaIII NlaIV Sau96I ScrFI SfaNI TseI