################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AocI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BamHI BbrPI BcgI BciVI BclI BfaI BfrI BglII BlnI BsaAI BsaBI BsaWI BseAI BsgI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DpnII DraI DraIII Eam1105I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FspI HincII HindII HindIII HpaI KpnI MaeI MaeII MamI MboI MfeI MflI MluI MroI MslI MunI NdeI NdeII NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SalI SapI Sau3AI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TfiI Tsp509I Tth111I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AlwNI 808 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 113 AGCGCT AGC^GCT BalI 353 TGGCCA TGG^CCA BbsI 763 GTCTTC GAAGAC(2/6) BbvCI 575 CCTCAGC CCTCAGC(-5/-2) BlpI 109 GCTGAGC GC^T[ACGT]AGC BpmI 469 CTCCAG CTGGAG(16/14) Bpu1102I 109 GCTGAGC GC^T[ACGT]AGC BpuAI 763 GTCTTC GAAGAC(2/6) BsiEI 781 CGGCCG CG[GA][CT]^CG CelII 109 GCTGAGC GC^T[ACGT]AGC DrdI 754 GACACCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 781 CGGCCG C^GGCCG EclXI 781 CGGCCG C^GGCCG Eco47III 113 AGCGCT AGC^GCT Eco52I 781 CGGCCG C^GGCCG EcoT14I 204 CCATGG C^C[AT][AT]GG FseI 29 GGCCGGCC GGCCGG^CC HinfI 493 GAGTC G^A[ACGT]TC HphI 16 GGTGA GGTGA(8/7) MaeIII 17 GTGAC ^GT[ACGT]AC MluNI 353 TGGCCA TGG^CCA MscI 353 TGGCCA TGG^CCA MseI 791 TTAA T^TAA NcoI 204 CCATGG C^CATGG NspI 722 GCATGC [GA]CATG^[CT] PleI 493 GAGTC GAGTC(4/5) PvuII 415 CAGCTG CAG^CTG SfaNI 710 GCATC GCATC(5/9) SmaI 180 CCCGGG CCC^GGG SphI 722 GCATGC GCATG^C StyI 204 CCATGG C^C[AT][AT]GG TaqI 210 TCGA T^CGA Tru9I 791 TTAA T^TAA Tsp45I 17 GTGAC ^GT[GC]AC TthHB8I 210 TCGA T^CGA XmaCI 180 CCCGGG C^CCGGG XmaI 180 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AfaI 509 GTAC GT^AC 597 GTAC ApaI 122 GGGCCC GGGCC^C 183 GGGCCC BanI 22 GGCGCC G^G[CT][GA]CC 71 GGCGCC BbeI 22 GGCGCC GGCGC^C 71 GGCGCC BsaI 400 GAGACC GGTCTC(1/5) 535 GAGACC BspMI 556 ACCTGC ACCTGC(4/8) 773 ACCTGC BsrFI 30 GCCGGC [GA]^CCGG[CT] 544 GCCGGC Cfr10I 30 GCCGGC [GA]^CCGG[CT] 544 GCCGGC EarI 690 CTCTTC CTCTTC(1/4) 645 GAAGAG EcoNI 219 CCTGGCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 525 CCTGAGCGAGG HgaI 460 GACGC GACGC(5/10) 64 GCGTC KasI 22 GGCGCC G^GCGCC 71 GGCGCC Ksp632I 690 CTCTTC CTCTTC(1/4) 645 GAAGAG KspI 155 CCGCGG CCGC^GG 784 CCGCGG NaeI 30 GCCGGC GCC^GGC 544 GCCGGC NarI 22 GGCGCC GG^CGCC 71 GGCGCC NgoMIV 30 GCCGGC G^CCGGC 544 GCCGGC PpuMI 473 AGGACCT [GA]G^G[AT]CC[CT] 770 GGGACCT RsaI 509 GTAC GT^AC 597 GTAC SacII 155 CCGCGG CCGC^GG 784 CCGCGG SfoI 22 GGCGCC GGC^GCC 71 GGCGCC ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AccII AciI AcyI AluI AvaI AvaII BanII BbvI BcnI BglI BmyI BsaHI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssHII BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII ItaI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV Sau96I ScrFI TseI TspRI