(Circular) (Six-base) MAPSORT of: pFN21AE3106.seq Check: 6848 from: 1 to: 5533 pFN21AE3106 5533 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2570 2570 Size: 5533 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3343 278 Size: 53 83 186 936 1807 2468 Fragments arranged by size: 2468 1807 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2876 1937 Size: 939 4594 Acc65I G'GTAC_C Cuts at: 2854 2854 Size: 5533 AclI AA'CG_TT Cuts at: 1563 3660 4033 1563 Size: 2097 373 3063 Fragments arranged by size: 3063 2097 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2840 3596 4980 5248 1632 Size: 1208 756 1384 268 1917 Fragments arranged by size: 1917 1384 1208 756 268 AfeI AGC'GCT Cuts at: 2120 2120 Size: 5533 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5336 AflIII A'CryG_T Cuts at: 1203 4432 5409 1203 Size: 3229 977 1327 Fragments arranged by size: 3229 1327 977 AgeI A'CCGG_T Cuts at: 4364 4364 Size: 5533 AhdI GACnn_n'nnGTC Cuts at: 4262 4262 Size: 5533 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5533 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5533 AlwNI CAG_nnn'CTG Cuts at: 2818 3276 4848 2818 Size: 458 1572 3503 Fragments arranged by size: 3503 1572 458 ApaI G_GGCC'C Cuts at: 2131 2192 2131 Size: 61 5472 ApaLI G'TGCA_C Cuts at: 3590 4746 3590 Size: 1156 4377 ApoI r'AATT_y Cuts at: 1493 2842 3068 3220 5329 1493 Size: 1349 226 152 2109 1697 Fragments arranged by size: 2109 1697 1349 226 152 AseI AT'TA_AT Cuts at: 160 1032 4087 160 Size: 872 3055 1606 Fragments arranged by size: 3055 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5533 AvaI C'yCGr_G Cuts at: 1870 1943 2081 2185 2507 2858 1870 Size: 73 138 104 322 351 4545 Fragments arranged by size: 4545 351 322 138 104 73 BamHI G'GATC_C Cuts at: 2863 3230 2863 Size: 367 5166 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2027 2076 2854 4309 618 Size: 325 217 627 240 49 778 1455 1842 Fragments arranged by size: 1842 1455 778 627 325 240 217 49 BanII G_rGCy'C Cuts at: 1391 2131 2192 2224 2394 2448 2852 1391 Size: 740 61 32 170 54 404 4072 Fragments arranged by size: 4072 740 404 170 61 54 32 BbeI G_GCGC'C Cuts at: 2031 2080 2031 Size: 49 5484 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2761 928 Size: 971 862 3700 Fragments arranged by size: 3700 971 862 BbvCI CC'TCA_GC Cuts at: 2581 2581 Size: 5533 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3724 3758 1903 Size: 34 1787 34 3678 Fragments arranged by size: 3678 1787 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3425 4635 3425 Size: 1210 4323 BclI T'GATC_A Cuts at: 1548 1548 Size: 5533 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2036 2045 2273 Size: 107 122 71 631 605 364 9 228 Cuts at: 2273 4144 136 Size: 1871 1525 Fragments arranged by size: 1871 1525 631 605 364 228 122 107 71 9 BglII A'GATC_T Cuts at: 5528 5528 Size: 5533 BlpI GC'TnA_GC Cuts at: 2115 2950 2115 Size: 835 4698 Bme1580I G_kGCm'C Cuts at: 1790 2057 2131 2192 2574 2631 3594 4750 1790 Size: 267 74 61 382 57 963 1156 2573 Fragments arranged by size: 2573 1156 963 382 267 74 61 57 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5533 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2319 3264 4222 Size: 416 53 83 399 24 902 945 958 Cuts at: 4222 442 Size: 1753 Fragments arranged by size: 1753 958 945 902 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5533 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2459 4193 1239 Size: 1220 1734 2579 Fragments arranged by size: 2579 1734 1220 Bpu10I CC'TnA_GC Cuts at: 2531 2581 2531 Size: 50 5483 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3658 4523 4821 3658 Size: 865 298 4370 Fragments arranged by size: 4370 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2399 2534 4196 882 Size: 617 900 135 1662 2219 Fragments arranged by size: 2219 1662 900 617 135 BsaAI yAC'GTr Cuts at: 493 1204 3324 493 Size: 711 2120 2702 Fragments arranged by size: 2702 2120 711 BsaBI GATnn'nnATC Cuts at: 5527 5527 Size: 5533 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2028 2069 2077 2466 Size: 53 83 186 936 495 41 8 389 Cuts at: 2466 3340 3722 4357 275 Size: 874 382 635 1451 Fragments arranged by size: 1451 936 874 635 495 389 382 186 83 53 41 8 BsaWI w'CCGG_w Cuts at: 3966 4364 4638 4785 5196 3966 Size: 398 274 147 411 4303 Fragments arranged by size: 4303 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5308 Fragments arranged by size: 5308 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2099 2232 2390 2552 2693 1186 Size: 913 133 158 162 141 4026 Fragments arranged by size: 4026 913 162 158 141 133 BseYI C'CCAG_C Cuts at: 2336 2422 2575 4736 5232 2336 Size: 86 153 2161 496 2637 Fragments arranged by size: 2637 2161 496 153 86 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4960 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2789 2983 3744 4772 1540 Size: 275 186 788 194 761 1028 2301 Fragments arranged by size: 2301 1028 788 761 275 194 186 BsiHKAI G_wGCw'C Cuts at: 3594 3679 4750 3594 Size: 85 1071 4377 Fragments arranged by size: 4377 1071 85 BsmI GAATG_Cn' Cuts at: 3044 3137 3044 Size: 93 5440 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2057 2131 2192 2224 2394 2448 2574 Size: 399 267 74 61 32 170 54 126 Cuts at: 2574 2631 2852 3594 3679 4750 1391 Size: 57 221 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 267 221 170 126 85 74 61 57 54 32 BspEI T'CCGG_A Cuts at: 5196 5196 Size: 5533 BspHI T'CATG_A Cuts at: 3422 5152 3422 Size: 1730 3803 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2570 2787 2888 844 Size: 1726 217 101 3489 Fragments arranged by size: 3489 1726 217 101 BsrBI CCG'CTC Cuts at: 1394 1823 3420 1394 Size: 429 1597 3507 Fragments arranged by size: 3507 1597 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4028 4202 65 Size: 1183 2780 174 1396 Fragments arranged by size: 2780 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2035 2549 4177 4364 1700 Size: 110 225 514 1628 187 2869 Fragments arranged by size: 2869 1628 514 225 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5533 BssHII G'CGCG_C Cuts at: 1925 2237 2731 2733 1925 Size: 312 494 2 4725 Fragments arranged by size: 4725 494 312 2 BssSI C'ACGA_G Cuts at: 3593 4605 3593 Size: 1012 4521 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5533 BstBI TT'CG_AA Cuts at: 4337 4337 Size: 5533 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5533 BstYI r'GATC_y Cuts at: 1972 2863 3230 3615 3632 5084 5170 5182 5528 Size: 891 367 385 17 1452 86 12 346 Cuts at: 5528 1972 Size: 1977 Fragments arranged by size: 1977 1452 891 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2100 2160 2209 2783 2789 513 Size: 552 1035 60 49 574 6 3257 Fragments arranged by size: 3257 1035 574 552 60 49 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2242 2701 487 Size: 683 56 1016 459 3319 Fragments arranged by size: 3319 1016 683 459 56 BtsI GCAGTG_nn' Cuts at: 2280 3049 3843 3863 2280 Size: 769 794 20 3950 Fragments arranged by size: 3950 794 769 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5533 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5498 DraI TTT'AAA Cuts at: 2837 2837 Size: 5533 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2765 4540 809 Size: 507 1449 1775 1802 Fragments arranged by size: 1802 1775 1449 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2037 2358 2786 Size: 54 1425 50 275 18 207 321 428 Cuts at: 2786 2980 3869 5498 8 Size: 194 889 1629 43 Fragments arranged by size: 1629 1425 889 428 321 275 207 194 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2786 2980 1537 Size: 275 974 194 4090 Fragments arranged by size: 4090 974 275 194 EarI CTCTTCn'nnn_ Cuts at: 1353 2645 2701 3463 1353 Size: 1292 56 762 3423 Fragments arranged by size: 3423 1292 762 56 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2243 4120 4494 4640 387 Size: 1064 138 654 1877 374 146 1280 Fragments arranged by size: 1877 1280 1064 654 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2459 2840 3596 4193 4980 5248 1239 Size: 393 827 381 756 597 787 268 1524 Fragments arranged by size: 1524 827 787 756 597 393 381 268 EcoNI CCTnn'n_nnAGG Cuts at: 2228 2534 2228 Size: 306 5227 EcoO109I rG'GnC_Cy Cuts at: 2189 2479 2776 2189 Size: 290 297 4946 Fragments arranged by size: 4946 297 290 EcoRI G'AATT_C Cuts at: 2842 3220 2842 Size: 378 5155 FseI GG_CCGG'CC Cuts at: 2039 2039 Size: 5533 FspI TGC'GCA Cuts at: 4039 4039 Size: 5533 HaeII r_GCGC'y Cuts at: 1678 2031 2080 2122 4680 1678 Size: 353 49 42 2558 2531 Fragments arranged by size: 2558 2531 353 49 42 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2651 2683 4174 4206 4248 Size: 32 135 32 1079 32 1491 32 42 Cuts at: 4248 4280 1373 Size: 32 2626 Fragments arranged by size: 2626 1491 1079 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2877 3123 669 Size: 988 281 939 246 3079 Fragments arranged by size: 3079 988 939 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5533 HpaI GTT'AAC Cuts at: 3123 3123 Size: 5533 KasI G'GCGC_C Cuts at: 2027 2076 2027 Size: 49 5484 KpnI G_GTAC'C Cuts at: 2858 2858 Size: 5533 MfeI C'AATT_G Cuts at: 3132 3132 Size: 5533 MluI A'CGCG_T Cuts at: 5409 5409 Size: 5533 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4647 4831 1305 Size: 3342 184 2007 Fragments arranged by size: 3342 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2360 10 Size: 54 1425 343 528 3183 Fragments arranged by size: 3183 1425 528 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3491 3850 4009 518 Size: 648 2325 359 159 2042 Fragments arranged by size: 2325 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2114 2162 2422 2791 2949 3626 4774 5019 Size: 508 48 260 369 158 677 1148 245 Cuts at: 5019 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 508 369 260 245 158 48 NaeI GCC'GGC Cuts at: 2037 2551 2037 Size: 514 5019 NarI GG'CG_CC Cuts at: 2028 2077 2028 Size: 49 5484 NcoI C'CATG_G Cuts at: 513 1065 2209 513 Size: 552 1144 3837 Fragments arranged by size: 3837 1144 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5533 NgoMIV G'CCGG_C Cuts at: 2035 2549 2035 Size: 514 5019 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5533 NotI GC'GGCC_GC Cuts at: 2980 2980 Size: 5533 NspI r_CATG'y Cuts at: 1132 1229 2731 2891 4436 1132 Size: 97 1502 160 1545 2229 Fragments arranged by size: 2229 1545 1502 160 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5533 PciI A'CATG_T Cuts at: 4432 4432 Size: 5533 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5533 PfoI T'CCnGG_A Cuts at: 2475 2475 Size: 5533 PmeI GTTT'AAAC Cuts at: 2837 2837 Size: 5533 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3602 3634 5115 5147 3602 Size: 32 1481 32 3988 Fragments arranged by size: 3988 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2479 2776 2479 Size: 297 5236 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5533 PsiI TTA'TAA Cuts at: 3103 3103 Size: 5533 PspOMI G'GGCC_C Cuts at: 2127 2188 2127 Size: 61 5472 PstI C_TGCA'G Cuts at: 830 2885 830 Size: 2055 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5533 PvuII CAG'CTG Cuts at: 2422 2422 Size: 5533 SacII CC_GC'GG Cuts at: 2163 2792 2163 Size: 629 4904 SalI G'TCGA_C Cuts at: 1936 2875 1936 Size: 939 4594 SbfI CC_TGCA'GG Cuts at: 2885 2885 Size: 5533 ScaI AGT'ACT Cuts at: 3781 5493 3781 Size: 1712 3821 SfcI C'TryA_G Cuts at: 826 1045 2881 4016 4697 4888 826 Size: 219 1836 1135 681 191 1471 Fragments arranged by size: 1836 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2029 2078 2029 Size: 49 5484 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5533 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5533 SmaI CCC'GGG Cuts at: 1872 2187 2860 1872 Size: 315 673 4545 Fragments arranged by size: 4545 673 315 SmlI C'TyrA_G Cuts at: 820 1017 1943 3637 4538 4800 820 Size: 197 926 1694 901 262 1553 Fragments arranged by size: 1694 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3324 493 Size: 2831 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5533 SphI G_CATG'C Cuts at: 1132 2731 2891 1132 Size: 1599 160 3774 Fragments arranged by size: 3774 1599 160 SrfI GCCC'GGGC Cuts at: 2187 2187 Size: 5533 SspI AAT'ATT Cuts at: 5 52 3457 5 Size: 47 3405 2081 Fragments arranged by size: 3405 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5533 StyI C'CwwG_G Cuts at: 513 1065 2209 2972 513 Size: 552 1144 763 3074 Fragments arranged by size: 3074 1144 763 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2338 2350 2777 3560 3577 3730 3915 Size: 1255 758 12 427 783 17 153 185 Cuts at: 3915 4360 325 Size: 445 1498 Fragments arranged by size: 1498 1255 783 758 445 427 185 153 17 12 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2513 3779 5491 Size: 274 80 33 51 1443 536 1266 1712 Cuts at: 5491 96 Size: 138 Fragments arranged by size: 1712 1443 1266 536 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5493 5525 5493 Size: 32 5501 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5533 XbaI T'CTAG_A Cuts at: 2869 2869 Size: 5533 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5533 XmaI C'CCGG_G Cuts at: 1870 2185 2858 1870 Size: 315 673 4545 Fragments arranged by size: 4545 673 315 XmnI GAAnn'nnTTC Cuts at: 3662 3662 Size: 5533 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3341 276 Size: 53 83 186 936 1807 2468 Fragments arranged by size: 2468 1807 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SwaI XcmI