################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII Acc65I AccII AccIII AcsI AcyI AflII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII BbeI BbvCI BclI BfrI BglII BlpI Bpu1102I BsaBI BsaHI BsaI BseAI BsiEI BsiWI Bsp1407I BspEI BspHI BspLU11I BspMI BsrDI BsrGI BssHII BstEII BstPI BstUI CelII CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoT22I FbaI FseI FspI HaeII Hin1I HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmeI PpuMI PshBI PvuI RcaI RsrII SacII SalI SbfI ScaI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tsp509I Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AclI 1079 AACGTT AA^CGTT AflIII 656 ACACGT A^C[GA][CT]GT AocI 218 CCTCAGG CC^T[ACGT]AGG ApaI 95 GGGCCC GGGCC^C BalI 1548 TGGCCA TGG^CCA BamHI 70 GGATCC G^GATCC BbrPI 657 CACGTG CAC^GTG BbsI 856 GTCTTC GAAGAC(2/6) BcgI 1323 GCAGAAAACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 886 GGATAC GTATCC(6/5) BpuAI 856 GTCTTC GAAGAC(2/6) BsaWI 394 ACCGGA [AT]^CCGG[AT] BsgI 410 CTGCAC GTGCAG(16/14) BsmBI 453 GAGACG CGTCTC(1/5) BspDI 1391 ATCGAT AT^CGAT BsrBI 931 GAGCGG CCGCTC(-3/-3) BsrFI 1568 GCCGGT [GA]^CCGG[CT] Bst1107I 1501 GTATAC GTA^TAC BstAPI 738 GCAAGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 641 TTCGAA TT^CGAA BstXI 1152 CCAGGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 70 GGATCC [GA]^GATC[CT] BstZ17I 1501 GTATAC GTA^TAC Bsu36I 218 CCTCAGG CC^T[ACGT]AGG CfoI 111 GCGC GCG^C Cfr10I 1568 GCCGGT [GA]^CCGG[CT] ClaI 1391 ATCGAT AT^CGAT DrdI 1600 GACTCTCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 218 CCTCAGG CC^T[ACGT]AGG EcoNI 748 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 555 GATATC GAT^ATC HhaI 111 GCGC GCG^C HinP1I 111 GCGC G^CGC HincII 1347 GTTGAC GT[CT]^[GA]AC HindII 1347 GTTGAC GT[CT]^[GA]AC HphI 277 TCACC GGTGA(8/7) MflI 70 GGATCC [GA]^GATC[CT] MluNI 1548 TGGCCA TGG^CCA MscI 1548 TGGCCA TGG^CCA MslI 1682 CACCCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 1507 CAGCTG C[AC]G^C[GT]G NcoI 537 CCATGG C^CATGG NspI 1587 GCATGT [GA]CATG^[CT] NspV 641 TTCGAA TT^CGAA PflMI 121 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 657 CACGTG CAC^GTG PmlI 657 CACGTG CAC^GTG Psp1406I 1079 AACGTT AA^CGTT PvuII 1507 CAGCTG CAG^CTG SacI 769 GAGCTC GAGCT^C SapI 1279 GAAGAGC GCTCTTC(1/4) SexAI 1628 ACCAGGT A^CC[AT]GGT SfuI 641 TTCGAA TT^CGAA Van91I 121 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 70 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AlwI 71 GATCC GGATC(4/5) 70 GGATC AvrII 477 CCTAGG C^CTAGG 1464 CCTAGG BlnI 477 CCTAGG C^CTAGG 1464 CCTAGG BsaAI 657 CACGTG [CT]AC^GT[GA] 1651 TACGTG BsmI 669 GAATGC GAATGC(1/-1) 1296 GAATGC BssSI 1375 CTCGTG CACGAG(-5/-1) 1604 CTCGTG Cac8I 97 GCCCGC GC[ACGT]^[ACGT]GC 893 GCCAGC DpnII 71 GATC ^GATC 253 GATC DsaI 387 CCGTGG C^C[GA][CT]GG 537 CCATGG EaeI 163 CGGCCA [CT]^GGCC[GA] 1548 TGGCCA EarI 704 CTCTTC CTCTTC(1/4) 1279 GAAGAG HgaI 357 GCGTC GACGC(5/10) 1538 GCGTC Ksp632I 704 CTCTTC CTCTTC(1/4) 1279 GAAGAG MboI 71 GATC ^GATC 253 GATC MseI 50 TTAA T^TAA 1045 TTAA NdeII 71 GATC ^GATC 253 GATC PstI 679 CTGCAG CTGCA^G 1450 CTGCAG Sau3AI 71 GATC ^GATC 253 GATC SfcI 679 CTGCAG C^T[GA][CT]AG 1450 CTGCAG SmlI 187 CTCAAG C^T[CT][GA]AG 1144 CTCAAG StuI 8 AGGCCT AGG^CCT 1154 AGGCCT Tru9I 50 TTAA T^TAA 1045 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AccI AciI AfaI AluI AlwNI AspHI AvaI AvaII BanI BanII BbvI BcnI BfaI BglI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI Cfr13I DdeI DraII EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MaeI MaeII MaeIII MboII MnlI MspI MvaI MwoI NciI NlaIII NlaIV PleI PshAI RsaI Sau96I ScrFI SfaNI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I