(Circular) (Six-base) MAPSORT of: pFN21AE3242.seq Check: 6174 from: 1 to: 6400 pFN21AE3242 6400 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4210 278 Size: 53 83 186 936 2674 2468 Fragments arranged by size: 2674 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3444 3507 3597 3743 1937 Size: 1507 63 90 146 4594 Fragments arranged by size: 4594 1507 146 90 63 Acc65I G'GTAC_C Cuts at: 3721 3721 Size: 6400 AclI AA'CG_TT Cuts at: 1563 3085 4527 4900 1563 Size: 1522 1442 373 3063 Fragments arranged by size: 3063 1522 1442 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2802 2942 4463 5847 6115 1632 Size: 1170 140 1521 1384 268 1917 Fragments arranged by size: 1917 1521 1384 1170 268 140 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6203 AflIII A'CryG_T Cuts at: 1203 2661 5299 6276 1203 Size: 1458 2638 977 1327 Fragments arranged by size: 2638 1458 1327 977 AgeI A'CCGG_T Cuts at: 5231 5231 Size: 6400 AhdI GACnn_n'nnGTC Cuts at: 5129 5129 Size: 6400 AleI CACnn'nnGTG Cuts at: 1166 3691 1166 Size: 2525 3875 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6400 AlwNI CAG_nnn'CTG Cuts at: 2132 2692 3463 4143 5715 2132 Size: 560 771 680 1572 2817 Fragments arranged by size: 2817 1572 771 680 560 ApaI G_GGCC'C Cuts at: 2104 2104 Size: 6400 ApaLI G'TGCA_C Cuts at: 4457 5613 4457 Size: 1156 5244 ApoI r'AATT_y Cuts at: 1493 3709 3935 4087 6196 1493 Size: 2216 226 152 2109 1697 Fragments arranged by size: 2216 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4954 160 Size: 872 3922 1606 Fragments arranged by size: 3922 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6400 AvaI C'yCGr_G Cuts at: 1870 1943 2040 2067 3411 3725 1870 Size: 73 97 27 1344 314 4545 Fragments arranged by size: 4545 1344 314 97 73 27 AvrII C'CTAG_G Cuts at: 2482 3469 2482 Size: 987 5413 BamHI G'GATC_C Cuts at: 2075 3730 4097 2075 Size: 1655 367 4378 Fragments arranged by size: 4378 1655 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2388 2396 2749 2804 2814 Size: 325 217 627 601 8 353 55 10 Cuts at: 2814 3576 3693 3721 5176 618 Size: 762 117 28 1455 1842 Fragments arranged by size: 1842 1455 762 627 601 353 325 217 117 55 28 10 8 BanII G_rGCy'C Cuts at: 1391 2104 2532 2630 2770 2778 2977 3031 3719 Size: 713 428 98 140 8 199 54 688 Cuts at: 3719 1391 Size: 4072 Fragments arranged by size: 4072 713 688 428 199 140 98 54 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2854 928 Size: 971 955 4474 Fragments arranged by size: 4474 971 955 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3317 3351 4591 4625 1903 Size: 34 50 34 1296 34 1240 34 3678 Fragments arranged by size: 3678 1296 1240 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2885 4292 5502 2885 Size: 1407 1210 3783 Fragments arranged by size: 3783 1407 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6400 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2099 2167 2487 Size: 107 122 71 631 605 427 68 320 Cuts at: 2487 5011 136 Size: 2524 1525 Fragments arranged by size: 2524 1525 631 605 427 320 122 107 71 68 BglII A'GATC_T Cuts at: 6395 6395 Size: 6400 BlpI GC'TnA_GC Cuts at: 3817 3817 Size: 6400 Bme1580I G_kGCm'C Cuts at: 1790 2104 2391 2809 4461 5617 1790 Size: 314 287 418 1652 1156 2573 Fragments arranged by size: 2573 1652 1156 418 314 287 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6400 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3302 4131 5089 Size: 416 53 83 399 24 1885 829 958 Cuts at: 5089 442 Size: 1753 Fragments arranged by size: 1885 1753 958 829 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6400 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2718 2718 Size: 6400 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2222 2775 3113 5060 1239 Size: 983 553 338 1947 2579 Fragments arranged by size: 2579 1947 983 553 338 Bpu10I CC'TnA_GC Cuts at: 2442 2442 Size: 6400 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2177 3134 4525 5390 5688 2177 Size: 957 1391 865 298 2889 Fragments arranged by size: 2889 1391 957 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5063 882 Size: 617 3564 2219 Fragments arranged by size: 3564 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2664 3658 4191 493 Size: 711 1460 994 533 2702 Fragments arranged by size: 2702 1460 994 711 533 BsaBI GATnn'nnATC Cuts at: 6394 6394 Size: 6400 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4207 4589 5224 275 Size: 53 83 186 936 2674 382 635 1451 Fragments arranged by size: 2674 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2399 4833 5231 5505 5652 6063 2399 Size: 2434 398 274 147 411 2736 Fragments arranged by size: 2736 2434 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2807 2837 1178 Size: 30 165 30 1404 30 4741 Fragments arranged by size: 4741 1404 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2772 2918 3036 1186 Size: 1586 146 118 4550 Fragments arranged by size: 4550 1586 146 118 BseYI C'CCAG_C Cuts at: 2125 2206 3233 5603 6099 2125 Size: 81 1027 2370 496 2426 Fragments arranged by size: 2426 2370 1027 496 81 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2400 1163 Size: 573 664 5163 Fragments arranged by size: 5163 664 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3850 4611 5639 1540 Size: 275 186 1849 761 1028 2301 Fragments arranged by size: 2301 1849 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2025 2778 3387 4461 4546 5617 2025 Size: 753 609 1074 85 1071 2808 Fragments arranged by size: 2808 1074 1071 753 609 85 BsmI GAATG_Cn' Cuts at: 2680 3307 3911 4004 2680 Size: 627 604 93 5076 Fragments arranged by size: 5076 627 604 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2452 2452 Size: 6400 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2104 2391 2532 2630 2770 2778 Size: 399 235 79 287 141 98 140 8 Cuts at: 2778 2809 2977 3031 3387 3719 4461 4546 5617 Size: 31 168 54 356 332 742 85 1071 Cuts at: 5617 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 356 332 287 235 168 141 140 98 85 79 54 31 8 BspEI T'CCGG_A Cuts at: 6063 6063 Size: 6400 BspHI T'CATG_A Cuts at: 4289 6019 4289 Size: 1730 4670 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3755 844 Size: 2911 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2938 4287 1394 Size: 429 1115 1349 3507 Fragments arranged by size: 3507 1349 1115 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4895 5069 65 Size: 1183 3647 174 1396 Fragments arranged by size: 3647 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3573 5044 5231 1700 Size: 110 1763 1471 187 2869 Fragments arranged by size: 2869 1763 1471 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6400 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6400 BssSI C'ACGA_G Cuts at: 3380 3609 4460 5472 3380 Size: 229 851 1012 4308 Fragments arranged by size: 4308 1012 851 229 BstAPI GCAn_nnn'nTGC Cuts at: 794 2749 794 Size: 1955 4445 BstBI TT'CG_AA Cuts at: 2647 5204 2647 Size: 2557 3843 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6400 BstXI CCAn_nnnn'nTGG Cuts at: 3164 3164 Size: 6400 BstYI r'GATC_y Cuts at: 1972 2075 3730 4097 4482 4499 5951 6037 6049 Size: 103 1655 367 385 17 1452 86 12 Cuts at: 6049 6395 1972 Size: 346 1977 Fragments arranged by size: 1977 1655 1452 385 367 346 103 86 17 12 BstZ17I GTA'TAC Cuts at: 3508 3508 Size: 6400 Bsu36I CC'TnA_GG Cuts at: 2224 2224 Size: 6400 BtgI C'CryG_G Cuts at: 513 1065 2392 2542 513 Size: 552 1327 150 4371 Fragments arranged by size: 4371 1327 552 150 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5661 Fragments arranged by size: 5661 683 56 BtsI GCAGTG_nn' Cuts at: 2356 2693 3452 3916 4710 4730 2356 Size: 337 759 464 794 20 4026 Fragments arranged by size: 4026 794 759 464 337 20 ClaI AT'CG_AT Cuts at: 1554 3397 1554 Size: 1843 4557 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2436 2471 529 Size: 35 1872 35 4458 Fragments arranged by size: 4458 1872 35 35 DraI TTT'AAA Cuts at: 3704 3704 Size: 6400 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3611 5407 809 Size: 507 2295 1796 1802 Fragments arranged by size: 2295 1802 1796 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2168 3553 3847 Size: 54 1425 50 275 18 338 1385 294 Cuts at: 3847 4736 6365 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1385 889 338 294 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3847 1537 Size: 275 2035 4090 Fragments arranged by size: 4090 2035 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2715 3279 4330 1353 Size: 1362 564 1051 3423 Fragments arranged by size: 3423 1362 1051 564 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2465 2554 4987 5361 5507 387 Size: 1064 138 876 89 2433 374 146 1280 Fragments arranged by size: 2433 1280 1064 876 374 146 138 89 EcoICRI GAG'CTC Cuts at: 2776 2776 Size: 6400 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2222 2775 2802 2942 3113 4463 5060 Size: 393 590 553 27 140 171 1350 597 Cuts at: 5060 5847 6115 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1350 787 597 590 553 393 268 171 140 27 EcoNI CCTnn'n_nnAGG Cuts at: 2757 2757 Size: 6400 EcoO109I rG'GnC_Cy Cuts at: 2100 2228 2243 2100 Size: 128 15 6257 Fragments arranged by size: 6257 128 15 EcoRI G'AATT_C Cuts at: 3709 4087 3709 Size: 378 6022 EcoRV GAT'ATC Cuts at: 2562 2562 Size: 6400 FspI TGC'GCA Cuts at: 4906 4906 Size: 6400 HaeII r_GCGC'y Cuts at: 1678 5547 1678 Size: 3869 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2593 2625 3278 3310 3405 Size: 32 135 32 1021 32 653 32 95 Cuts at: 3405 3437 5041 5073 5115 5147 1373 Size: 32 1604 32 42 32 2626 Fragments arranged by size: 2626 1604 1021 653 135 95 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3354 3744 3990 669 Size: 988 281 1416 390 246 3079 Fragments arranged by size: 3079 1416 988 390 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6400 HpaI GTT'AAC Cuts at: 3990 3990 Size: 6400 KpnI G_GTAC'C Cuts at: 3725 3725 Size: 6400 MfeI C'AATT_G Cuts at: 3999 3999 Size: 6400 MluI A'CGCG_T Cuts at: 6276 6276 Size: 6400 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3276 5514 5698 1305 Size: 1971 2238 184 2007 Fragments arranged by size: 2238 2007 1971 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3555 10 Size: 54 1425 343 1723 2855 Fragments arranged by size: 2855 1723 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3691 4358 4717 4876 518 Size: 648 2525 667 359 159 2042 Fragments arranged by size: 2525 2042 667 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3514 3816 4493 5641 5886 1606 Size: 1908 302 677 1148 245 2120 Fragments arranged by size: 2120 1908 1148 677 302 245 NcoI C'CATG_G Cuts at: 513 1065 2542 513 Size: 552 1477 4371 Fragments arranged by size: 4371 1477 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6400 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6400 NotI GC'GGCC_GC Cuts at: 3847 3847 Size: 6400 NspI r_CATG'y Cuts at: 1132 1229 3596 3758 5303 1132 Size: 97 2367 162 1545 2229 Fragments arranged by size: 2367 2229 1545 162 97 PasI CC'CwG_GG Cuts at: 1348 2097 1348 Size: 749 5651 PciI A'CATG_T Cuts at: 5299 5299 Size: 6400 PflMI CCAn_nnn'nTGG Cuts at: 1108 2132 1108 Size: 1024 5376 PfoI T'CCnGG_A Cuts at: 2494 2494 Size: 6400 PmeI GTTT'AAAC Cuts at: 3704 3704 Size: 6400 PmlI CAC'GTG Cuts at: 2664 2664 Size: 6400 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4469 4501 5982 6014 4469 Size: 32 1481 32 4855 Fragments arranged by size: 4855 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2361 2721 3321 1613 Size: 748 360 600 4692 Fragments arranged by size: 4692 748 600 360 PsiI TTA'TAA Cuts at: 3970 3970 Size: 6400 PspOMI G'GGCC_C Cuts at: 2100 2100 Size: 6400 PstI C_TGCA'G Cuts at: 830 2688 3459 3752 830 Size: 1858 771 293 3478 Fragments arranged by size: 3478 1858 771 293 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6400 PvuII CAG'CTG Cuts at: 3514 3514 Size: 6400 SacI G_AGCT'C Cuts at: 2778 2778 Size: 6400 SalI G'TCGA_C Cuts at: 1936 3742 1936 Size: 1806 4594 SapI GCTCTTCn'nnn_ Cuts at: 3279 3279 Size: 6400 SbfI CC_TGCA'GG Cuts at: 3752 3752 Size: 6400 ScaI AGT'ACT Cuts at: 4648 6360 4648 Size: 1712 4688 SexAI A'CCwGG_T Cuts at: 3633 3633 Size: 6400 SfcI C'TryA_G Cuts at: 826 1045 2684 3455 3748 4883 5564 5755 826 Size: 219 1639 771 293 1135 681 191 1471 Fragments arranged by size: 1639 1471 1135 771 681 293 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6400 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6400 SmaI CCC'GGG Cuts at: 1872 3727 1872 Size: 1855 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2192 3149 4504 5405 5667 820 Size: 197 926 249 957 1355 901 262 1553 Fragments arranged by size: 1553 1355 957 926 901 262 249 197 SnaBI TAC'GTA Cuts at: 493 4191 493 Size: 3698 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6400 SphI G_CATG'C Cuts at: 1132 3758 1132 Size: 2626 3774 SspI AAT'ATT Cuts at: 5 52 4324 5 Size: 47 4272 2081 Fragments arranged by size: 4272 2081 47 StuI AGG'CCT Cuts at: 1517 2015 3161 1517 Size: 498 1146 4756 Fragments arranged by size: 4756 1146 498 StyI C'CwwG_G Cuts at: 513 1065 2379 2482 2542 3469 3839 513 Size: 552 1314 103 60 927 370 3074 Fragments arranged by size: 3074 1314 927 552 370 103 60 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2464 3078 3333 4427 4444 4597 4782 Size: 1255 884 614 255 1094 17 153 185 Cuts at: 4782 5227 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1094 884 614 445 255 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3358 4646 6358 Size: 274 80 33 51 1443 1381 1288 1712 Cuts at: 6358 96 Size: 138 Fragments arranged by size: 1712 1443 1381 1288 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2546 2578 2807 2839 6360 6392 2546 Size: 32 229 32 3521 32 2554 Fragments arranged by size: 3521 2554 229 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6400 XbaI T'CTAG_A Cuts at: 3736 3736 Size: 6400 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6400 XmaI C'CCGG_G Cuts at: 1870 3725 1870 Size: 1855 4545 XmnI GAAnn'nnTTC Cuts at: 4529 4529 Size: 6400 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4208 276 Size: 53 83 186 936 2674 2468 Fragments arranged by size: 2674 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI BsiWI DraIII FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI