Restriction map for
pFN21AE3242
(Linear) (Six-base) MAPSORT of: KIEE3242.seq Check: 1492 from: 1 to: 1695
KIEE3242.orf
With 182 enzymes: SgfI *
December 27, 2011 16:34 ..
AccI GT'mk_AC
Cuts at: 0 1439 1502 1592 1695
Size: 1439 63 90 103
Fragments arranged by size:
1439 103 90 63
AclI AA'CG_TT
Cuts at: 0 1080 1695
Size: 1080 615
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 797 937 1695
Size: 797 140 758
Fragments arranged by size:
797 758 140
AflIII A'CryG_T
Cuts at: 0 656 1695
Size: 656 1039
AleI CACnn'nnGTG
Cuts at: 0 1686 1695
Size: 1686 9
AlwNI CAG_nnn'CTG
Cuts at: 0 127 687 1458 1695
Size: 127 560 771 237
Fragments arranged by size:
771 560 237 127
ApaI G_GGCC'C
Cuts at: 0 99 1695
Size: 99 1596
AvaI C'yCGr_G
Cuts at: 0 35 62 1406 1695
Size: 35 27 1344 289
Fragments arranged by size:
1344 289 35 27
AvrII C'CTAG_G
Cuts at: 0 477 1464 1695
Size: 477 987 231
Fragments arranged by size:
987 477 231
BamHI G'GATC_C
Cuts at: 0 70 1695
Size: 70 1625
BanI G'GyrC_C
Cuts at: 0 383 391 744 799 809 1571 1688 1695
Size: 383 8 353 55 10 762 117 7
Fragments arranged by size:
762 383 353 117 55 10 8 7
BanII G_rGCy'C
Cuts at: 0 99 527 625 765 773 972 1026 1695
Size: 99 428 98 140 8 199 54 669
Fragments arranged by size:
669 428 199 140 99 98 54 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 849 1695
Size: 849 846
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1312 1346 1695
Size: 1312 34 349
Fragments arranged by size:
1312 349 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 880 1695
Size: 880 815
BglI GCCn_nnn'nGGC
Cuts at: 0 94 162 482 1695
Size: 94 68 320 1213
Fragments arranged by size:
1213 320 94 68
Bme1580I G_kGCm'C
Cuts at: 0 99 386 804 1695
Size: 99 287 418 891
Fragments arranged by size:
891 418 287 99
BmrI ACTGGGnnnn_n'
Cuts at: 0 1297 1695
Size: 1297 398
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 713 1695
Size: 713 982
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 770 1108 1695
Size: 217 553 338 587
Fragments arranged by size:
587 553 338 217
Bpu10I CC'TnA_GC
Cuts at: 0 437 1695
Size: 437 1258
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 1129 1695
Size: 172 957 566
Fragments arranged by size:
957 566 172
BsaAI yAC'GTr
Cuts at: 0 659 1653 1695
Size: 659 994 42
Fragments arranged by size:
994 659 42
BsaWI w'CCGG_w
Cuts at: 0 394 1695
Size: 394 1301
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 802 832 1695
Size: 802 30 863
Fragments arranged by size:
863 802 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 767 913 1031 1695
Size: 767 146 118 664
Fragments arranged by size:
767 664 146 118
BseYI C'CCAG_C
Cuts at: 0 120 201 1228 1695
Size: 120 81 1027 467
Fragments arranged by size:
1027 467 120 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 395 1695
Size: 395 1300
BsiHKAI G_wGCw'C
Cuts at: 0 20 773 1382 1695
Size: 20 753 609 313
Fragments arranged by size:
753 609 313 20
BsmI GAATG_Cn'
Cuts at: 0 675 1302 1695
Size: 675 627 393
Fragments arranged by size:
675 627 393
BsmBI CGTCTCn'nnnn_
Cuts at: 0 447 1695
Size: 447 1248
Bsp1286I G_dGCh'C
Cuts at: 0 20 99 386 527 625 765 773 804
Size: 20 79 287 141 98 140 8 31
Cuts at: 804 972 1026 1382 1695
Size: 168 54 356 313
Fragments arranged by size:
356 313 287 168 141 140 98 79
54 31 20 8
BsrBI CCG'CTC
Cuts at: 0 933 1695
Size: 933 762
BsrFI r'CCGG_y
Cuts at: 0 1568 1695
Size: 1568 127
BssSI C'ACGA_G
Cuts at: 0 1375 1604 1695
Size: 1375 229 91
Fragments arranged by size:
1375 229 91
BstAPI GCAn_nnn'nTGC
Cuts at: 0 744 1695
Size: 744 951
BstBI TT'CG_AA
Cuts at: 0 642 1695
Size: 642 1053
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1159 1695
Size: 1159 536
BstYI r'GATC_y
Cuts at: 0 70 1695
Size: 70 1625
BstZ17I GTA'TAC
Cuts at: 0 1503 1695
Size: 1503 192
Bsu36I CC'TnA_GG
Cuts at: 0 219 1695
Size: 219 1476
BtgI C'CryG_G
Cuts at: 0 387 537 1695
Size: 387 150 1158
Fragments arranged by size:
1158 387 150
BtsI GCAGTG_nn'
Cuts at: 0 351 688 1447 1695
Size: 351 337 759 248
Fragments arranged by size:
759 351 337 248
ClaI AT'CG_AT
Cuts at: 0 1392 1695
Size: 1392 303
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 431 466 1695
Size: 431 35 1229
Fragments arranged by size:
1229 431 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1606 1695
Size: 1606 89
EaeI y'GGCC_r
Cuts at: 0 163 1548 1695
Size: 163 1385 147
Fragments arranged by size:
1385 163 147
EarI CTCTTCn'nnn_
Cuts at: 0 710 1274 1695
Size: 710 564 421
Fragments arranged by size:
710 564 421
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 460 549 1695
Size: 460 89 1146
Fragments arranged by size:
1146 460 89
EcoICRI GAG'CTC
Cuts at: 0 771 1695
Size: 771 924
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 770 797 937 1108 1695
Size: 217 553 27 140 171 587
Fragments arranged by size:
587 553 217 171 140 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 752 1695
Size: 752 943
EcoO109I rG'GnC_Cy
Cuts at: 0 95 223 238 1695
Size: 95 128 15 1457
Fragments arranged by size:
1457 128 95 15
EcoRV GAT'ATC
Cuts at: 0 557 1695
Size: 557 1138
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 588 620 1273 1305 1400 1432 1695
Size: 588 32 653 32 95 32 263
Fragments arranged by size:
653 588 263 95 32 32 32
HincII GTy'rAC
Cuts at: 0 1349 1695
Size: 1349 346
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1271 1695
Size: 1271 424
MscI TGG'CCA
Cuts at: 0 1550 1695
Size: 1550 145
MslI CAynn'nnrTG
Cuts at: 0 1686 1695
Size: 1686 9
MspA1I CmG'CkG
Cuts at: 0 1509 1695
Size: 1509 186
NcoI C'CATG_G
Cuts at: 0 537 1695
Size: 537 1158
NspI r_CATG'y
Cuts at: 0 1591 1695
Size: 1591 104
PasI CC'CwG_GG
Cuts at: 0 92 1695
Size: 92 1603
PflMI CCAn_nnn'nTGG
Cuts at: 0 127 1695
Size: 127 1568
PfoI T'CCnGG_A
Cuts at: 0 489 1695
Size: 489 1206
PmlI CAC'GTG
Cuts at: 0 659 1695
Size: 659 1036
PshAI GACnn'nnGTC
Cuts at: 0 356 716 1316 1695
Size: 356 360 600 379
Fragments arranged by size:
600 379 360 356
PspOMI G'GGCC_C
Cuts at: 0 95 1695
Size: 95 1600
PstI C_TGCA'G
Cuts at: 0 683 1454 1695
Size: 683 771 241
Fragments arranged by size:
771 683 241
PvuII CAG'CTG
Cuts at: 0 1509 1695
Size: 1509 186
SacI G_AGCT'C
Cuts at: 0 773 1695
Size: 773 922
SapI GCTCTTCn'nnn_
Cuts at: 0 1274 1695
Size: 1274 421
SexAI A'CCwGG_T
Cuts at: 0 1628 1695
Size: 1628 67
SfcI C'TryA_G
Cuts at: 0 679 1450 1695
Size: 679 771 245
Fragments arranged by size:
771 679 245
SmlI C'TyrA_G
Cuts at: 0 187 1144 1695
Size: 187 957 551
Fragments arranged by size:
957 551 187
StuI AGG'CCT
Cuts at: 0 10 1156 1695
Size: 10 1146 539
Fragments arranged by size:
1146 539 10
StyI C'CwwG_G
Cuts at: 0 374 477 537 1464 1695
Size: 374 103 60 927 231
Fragments arranged by size:
927 374 231 103 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 459 1073 1328 1695
Size: 459 614 255 367
Fragments arranged by size:
614 459 367 255
TatI w'GTAC_w
Cuts at: 0 1353 1695
Size: 1353 342
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 541 573 802 834 1695
Size: 541 32 229 32 861
Fragments arranged by size:
861 541 229 32 32
Enzymes that do cut:
AccI AclI AcuI AflIII AleI AlwNI ApaI AvaI
AvrII BamHI BanI BanII BbsI BcgI BciVI BglI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BsrBI BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I
Bsu36I BtgI BtsI ClaI CspCI DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV Hin4I HincII
MmeI MscI MslI MspA1I NcoI NspI PasI PflMI
PfoI PmlI PshAI PspOMI PstI PvuII SacI SapI
SexAI SfcI SmlI StuI StyI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AgeI AhdI AlfI
AloI ApaLI ApoI AscI AseI AsiSI BaeI BbeI
BbvCI BclI BfrBI BglII BlpI BmgBI BmtI BsaI
BsaBI BsaHI BsiEI BsiWI BspEI BspHI BspMI BsrDI
BsrGI BssHII BstEII BtgZI DraI DraIII EagI EcoRI
FalI FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PmeI PpiI PpuMI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI