(Circular) (Six-base) MAPSORT of: pFN21AB7728.seq Check: 5869 from: 1 to: 6142 pFN21AB7728 6142 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2634 2925 2634 Size: 291 5851 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3952 278 Size: 53 83 186 936 2416 2468 Fragments arranged by size: 2468 2416 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2336 3300 3485 1937 Size: 399 964 185 4594 Fragments arranged by size: 4594 964 399 185 Acc65I G'GTAC_C Cuts at: 3463 3463 Size: 6142 AclI AA'CG_TT Cuts at: 1563 4269 4642 1563 Size: 2706 373 3063 Fragments arranged by size: 3063 2706 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3041 4205 5589 5857 1632 Size: 1409 1164 1384 268 1917 Fragments arranged by size: 1917 1409 1384 1164 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5945 AflIII A'CryG_T Cuts at: 1203 2586 5041 6018 1203 Size: 1383 2455 977 1327 Fragments arranged by size: 2455 1383 1327 977 AgeI A'CCGG_T Cuts at: 4973 4973 Size: 6142 AhdI GACnn_n'nnGTC Cuts at: 4871 4871 Size: 6142 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6142 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2078 1184 Size: 894 5248 AlwNI CAG_nnn'CTG Cuts at: 2353 3011 3139 3885 5457 2353 Size: 658 128 746 1572 3038 Fragments arranged by size: 3038 1572 746 658 128 ApaI G_GGCC'C Cuts at: 3314 3314 Size: 6142 ApaLI G'TGCA_C Cuts at: 4199 5355 4199 Size: 1156 4986 ApoI r'AATT_y Cuts at: 1493 2528 3451 3677 3829 5938 1493 Size: 1035 923 226 152 2109 1697 Fragments arranged by size: 2109 1697 1035 923 226 152 AseI AT'TA_AT Cuts at: 160 1032 4696 160 Size: 872 3664 1606 Fragments arranged by size: 3664 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6142 AvaI C'yCGr_G Cuts at: 1870 1943 2027 2117 3030 3467 1870 Size: 73 84 90 913 437 4545 Fragments arranged by size: 4545 913 437 90 84 73 BamHI G'GATC_C Cuts at: 2388 3069 3472 3839 2388 Size: 681 403 367 4691 Fragments arranged by size: 4691 681 403 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3463 4918 618 Size: 325 217 627 1676 1455 1842 Fragments arranged by size: 1842 1676 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3314 3461 1391 Size: 1923 147 4072 Fragments arranged by size: 4072 1923 147 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2305 2693 928 Size: 971 406 388 4377 Fragments arranged by size: 4377 971 406 388 BbvCI CC'TCA_GC Cuts at: 3383 3383 Size: 6142 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4333 4367 1903 Size: 34 50 34 2312 34 3678 Fragments arranged by size: 3678 2312 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4034 5244 4034 Size: 1210 4932 BclI T'GATC_A Cuts at: 1548 1548 Size: 6142 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3318 4753 136 Size: 107 122 71 631 605 1646 1435 1525 Fragments arranged by size: 1646 1525 1435 631 605 122 107 71 BglII A'GATC_T Cuts at: 2272 3048 6137 2272 Size: 776 3089 2277 Fragments arranged by size: 3089 2277 776 BlpI GC'TnA_GC Cuts at: 3559 3559 Size: 6142 Bme1580I G_kGCm'C Cuts at: 1790 2568 3159 3314 4203 5359 1790 Size: 778 591 155 889 1156 2573 Fragments arranged by size: 2573 1156 889 778 591 155 BmgBI CAC'GTC Cuts at: 1318 2169 2791 1318 Size: 851 622 4669 Fragments arranged by size: 4669 851 622 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3873 4831 442 Size: 416 53 83 399 24 2456 958 1753 Fragments arranged by size: 2456 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6142 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2264 2375 3161 4802 1239 Size: 1025 111 786 1641 2579 Fragments arranged by size: 2579 1641 1025 786 111 Bpu10I CC'TnA_GC Cuts at: 3383 3383 Size: 6142 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4267 5132 5430 4267 Size: 865 298 4979 Fragments arranged by size: 4979 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4805 882 Size: 617 3306 2219 Fragments arranged by size: 3306 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3933 493 Size: 711 2729 2702 Fragments arranged by size: 2729 2702 711 BsaBI GATnn'nnATC Cuts at: 6136 6136 Size: 6142 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3949 4331 4966 275 Size: 53 83 186 936 2416 382 635 1451 Fragments arranged by size: 2416 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2385 4575 4973 5247 5394 5805 2385 Size: 2190 398 274 147 411 2722 Fragments arranged by size: 2722 2190 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3260 3290 1178 Size: 30 165 30 1857 30 4030 Fragments arranged by size: 4030 1857 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6142 BseYI C'CCAG_C Cuts at: 2106 2173 2257 2448 2946 3282 5345 5841 2106 Size: 67 84 191 498 336 2063 496 2407 Fragments arranged by size: 2407 2063 498 496 336 191 84 67 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2612 3371 1163 Size: 573 876 759 3934 Fragments arranged by size: 3934 876 759 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2677 3592 4353 5381 1540 Size: 275 186 676 915 761 1028 2301 Fragments arranged by size: 2301 1028 915 761 676 275 186 BsiHKAI G_wGCw'C Cuts at: 2034 2076 2346 2625 2777 4203 4288 5359 2034 Size: 42 270 279 152 1426 85 1071 2817 Fragments arranged by size: 2817 1426 1071 279 270 152 85 42 BsmI GAATG_Cn' Cuts at: 2461 2498 3653 3746 2461 Size: 37 1155 93 4857 Fragments arranged by size: 4857 1155 93 37 BsmBI CGTCTCn'nnnn_ Cuts at: 2189 2189 Size: 6142 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2034 2076 2346 2568 2625 2777 3159 Size: 399 244 42 270 222 57 152 382 Cuts at: 3159 3314 3461 4203 4288 5359 1391 Size: 155 147 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 382 270 244 222 155 152 147 85 57 42 BspEI T'CCGG_A Cuts at: 5805 5805 Size: 6142 BspHI T'CATG_A Cuts at: 4031 5761 4031 Size: 1730 4412 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2070 2634 2925 3497 844 Size: 1226 564 291 572 3489 Fragments arranged by size: 3489 1226 572 564 291 BsrBI CCG'CTC Cuts at: 1394 1823 4029 1394 Size: 429 2206 3507 Fragments arranged by size: 3507 2206 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3412 4637 4811 65 Size: 1183 2164 1225 174 1396 Fragments arranged by size: 2164 1396 1225 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2325 2985 3303 4786 4973 1700 Size: 110 515 660 318 1483 187 2869 Fragments arranged by size: 2869 1483 660 515 318 187 110 BsrGI T'GTAC_A Cuts at: 96 3267 96 Size: 3171 2971 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6142 BssSI C'ACGA_G Cuts at: 4202 5214 4202 Size: 1012 5130 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6142 BstBI TT'CG_AA Cuts at: 4946 4946 Size: 6142 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6142 BstXI CCAn_nnnn'nTGG Cuts at: 3053 3220 3053 Size: 167 5975 BstYI r'GATC_y Cuts at: 1972 2272 2388 3048 3069 3472 3839 4224 4241 Size: 300 116 660 21 403 367 385 17 Cuts at: 4241 5693 5779 5791 6137 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 660 403 385 367 346 300 116 86 21 17 12 BstZ17I GTA'TAC Cuts at: 2337 2337 Size: 6142 BtgI C'CryG_G Cuts at: 513 1065 2780 2901 3315 513 Size: 552 1715 121 414 3340 Fragments arranged by size: 3340 1715 552 414 121 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5403 Fragments arranged by size: 5403 683 56 BtsI GCAGTG_nn' Cuts at: 2512 2914 2957 3187 3658 4452 4472 2512 Size: 402 43 230 471 794 20 4182 Fragments arranged by size: 4182 794 471 402 230 43 20 ClaI AT'CG_AT Cuts at: 1554 2400 1554 Size: 846 5296 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6107 DraI TTT'AAA Cuts at: 3446 3446 Size: 6142 DraIII CAC_nnn'GTG Cuts at: 2772 2887 2772 Size: 115 6027 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5149 809 Size: 507 3833 1802 Fragments arranged by size: 3833 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2375 3589 4478 Size: 54 1425 50 275 18 545 1214 889 Cuts at: 4478 6107 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1214 889 545 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3589 1537 Size: 275 1777 4090 Fragments arranged by size: 4090 1777 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2015 2187 2480 3083 3219 4072 1353 Size: 662 172 293 603 136 853 3423 Fragments arranged by size: 3423 853 662 603 293 172 136 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4729 5103 5249 387 Size: 1064 138 3140 374 146 1280 Fragments arranged by size: 3140 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2264 2375 3041 3161 4205 4802 5589 Size: 393 632 111 666 120 1044 597 787 Cuts at: 5589 5857 1239 Size: 268 1524 Fragments arranged by size: 1524 1044 787 666 632 597 393 268 120 111 EcoO109I rG'GnC_Cy Cuts at: 2476 2893 3310 3311 2476 Size: 417 417 1 5307 Fragments arranged by size: 5307 417 417 1 EcoRI G'AATT_C Cuts at: 2528 3451 3829 2528 Size: 923 378 4841 Fragments arranged by size: 4841 923 378 FspI TGC'GCA Cuts at: 4648 4648 Size: 6142 HaeII r_GCGC'y Cuts at: 1678 2814 5289 1678 Size: 1136 2475 2531 Fragments arranged by size: 2531 2475 1136 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3040 3072 4783 4815 4857 Size: 32 135 32 1468 32 1711 32 42 Cuts at: 4857 4889 1373 Size: 32 2626 Fragments arranged by size: 2626 1711 1468 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3486 3732 669 Size: 988 281 1548 246 3079 Fragments arranged by size: 3079 1548 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6142 HpaI GTT'AAC Cuts at: 3732 3732 Size: 6142 KpnI G_GTAC'C Cuts at: 3467 3467 Size: 6142 MfeI C'AATT_G Cuts at: 3741 3741 Size: 6142 MluI A'CGCG_T Cuts at: 6018 6018 Size: 6142 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2260 2900 5256 5440 1305 Size: 955 640 2356 184 2007 Fragments arranged by size: 2356 2007 955 640 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4320 Fragments arranged by size: 4320 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2306 3326 4100 4459 4618 518 Size: 648 1140 1020 774 359 159 2042 Fragments arranged by size: 2042 1140 1020 774 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2110 2353 2452 2640 3558 4235 5383 5628 Size: 504 243 99 188 918 677 1148 245 Cuts at: 5628 1606 Size: 2120 Fragments arranged by size: 2120 1148 918 677 504 245 243 188 99 NaeI GCC'GGC Cuts at: 2327 2987 2327 Size: 660 5482 NcoI C'CATG_G Cuts at: 513 1065 2780 513 Size: 552 1715 3875 Fragments arranged by size: 3875 1715 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6142 NgoMIV G'CCGG_C Cuts at: 2325 2985 2325 Size: 660 5482 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6142 NotI GC'GGCC_GC Cuts at: 3589 3589 Size: 6142 NspI r_CATG'y Cuts at: 1132 1229 3500 5045 1132 Size: 97 2271 1545 2229 Fragments arranged by size: 2271 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2433 1348 Size: 1085 5057 PciI A'CATG_T Cuts at: 5041 5041 Size: 6142 PflMI CCAn_nnn'nTGG Cuts at: 1108 2353 2887 3331 1108 Size: 1245 534 444 3919 Fragments arranged by size: 3919 1245 534 444 PfoI T'CCnGG_A Cuts at: 2391 2937 3219 2391 Size: 546 282 5314 Fragments arranged by size: 5314 546 282 PmeI GTTT'AAAC Cuts at: 3446 3446 Size: 6142 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4211 4243 5724 5756 4211 Size: 32 1481 32 4597 Fragments arranged by size: 4597 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2476 2476 Size: 6142 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6142 PsiI TTA'TAA Cuts at: 3712 3712 Size: 6142 PspOMI G'GGCC_C Cuts at: 3310 3310 Size: 6142 PstI C_TGCA'G Cuts at: 830 2334 3165 3494 830 Size: 1504 831 329 3478 Fragments arranged by size: 3478 1504 831 329 PvuI CG_AT'CG Cuts at: 2001 2677 2001 Size: 676 5466 PvuII CAG'CTG Cuts at: 2110 2353 2452 2640 2110 Size: 243 99 188 5612 Fragments arranged by size: 5612 243 188 99 SalI G'TCGA_C Cuts at: 1936 3484 1936 Size: 1548 4594 SanDI GG'GwC_CC Cuts at: 2476 2476 Size: 6142 SapI GCTCTTCn'nnn_ Cuts at: 2015 3083 3219 2015 Size: 1068 136 4938 Fragments arranged by size: 4938 1068 136 SbfI CC_TGCA'GG Cuts at: 3494 3494 Size: 6142 ScaI AGT'ACT Cuts at: 4390 6102 4390 Size: 1712 4430 SexAI A'CCwGG_T Cuts at: 2799 2799 Size: 6142 SfcI C'TryA_G Cuts at: 826 1045 2330 3161 3490 4625 5306 5497 826 Size: 219 1285 831 329 1135 681 191 1471 Fragments arranged by size: 1471 1285 1135 831 681 329 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6142 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6142 SmaI CCC'GGG Cuts at: 1872 3469 1872 Size: 1597 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4246 5147 5409 820 Size: 197 926 2303 901 262 1553 Fragments arranged by size: 2303 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3933 493 Size: 3440 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6142 SphI G_CATG'C Cuts at: 1132 3500 1132 Size: 2368 3774 SspI AAT'ATT Cuts at: 5 52 4066 5 Size: 47 4014 2081 Fragments arranged by size: 4014 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6142 StyI C'CwwG_G Cuts at: 513 1065 2215 2780 3237 3581 513 Size: 552 1150 565 457 344 3074 Fragments arranged by size: 3074 1150 565 552 457 344 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4169 4186 4339 4524 4969 325 Size: 1255 2589 17 153 185 445 1498 Fragments arranged by size: 2589 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2613 3267 4388 Size: 274 80 33 51 1443 636 654 1121 Cuts at: 4388 6100 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1121 654 636 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6102 6134 6102 Size: 32 6110 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6142 XbaI T'CTAG_A Cuts at: 3478 3478 Size: 6142 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6142 XmaI C'CCGG_G Cuts at: 1870 3467 1870 Size: 1597 4545 XmnI GAAnn'nnTTC Cuts at: 4271 4271 Size: 6142 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3950 276 Size: 53 83 186 936 2416 2468 Fragments arranged by size: 2468 2416 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SfiI SfoI SrfI SwaI XcmI