Restriction map for
pFN21AE9394
##################################
# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AflIII AgeI Alw44I Aor51HI ApaLI
AscI AseI AsnI AspI AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BciVI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BseAI
BsiWI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BstBI BstEII BstPI BstUI CelII
Cfr10I ClaI CpoI DraI DrdI
EaeI EagI EclXI Eco47III Eco52I
EcoO65I FseI FspI HaeII HpaI
KasI KspI MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NdeI NgoMIV NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 2132 GGTACC G^GTACC
AcyI 76 GACGCC G[GA]^CG[CT]C
ApaI 3393 GGGCCC GGGCC^C
Asp718I 2132 GGTACC G^GTACC
BanII 3393 GGGCCC G[GA]GC[CT]^C
BclI 600 TGATCA T^GATCA
BsaHI 76 GACGCC G[GA]^CG[CT]C
BsaI 587 GGTCTC GGTCTC(1/5)
BsaWI 14 ACCGGA [AT]^CCGG[AT]
BsiEI 453 CGACCG CG[GA][CT]^CG
BspHI 3419 TCATGA T^CATGA
BspMI 412 GCAGGT ACCTGC(4/8)
Bst1107I 2818 GTATAC GTA^TAC
BstXI 831 CCAATCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2818 GTATAC GTA^TAC
CfoI 410 GCGC GCG^C
DraIII 1652 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 1732 CCTCGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 3483 GATATC GAT^ATC
FbaI 600 TGATCA T^GATCA
HhaI 410 GCGC GCG^C
Hin1I 76 GACGCC G[GA]^CG[CT]C
HinP1I 410 GCGC G^CGC
HincII 1893 GTCAAC GT[CT]^[GA]AC
HindII 1893 GTCAAC GT[CT]^[GA]AC
KpnI 2132 GGTACC GGTAC^C
MaeII 528 ACGT A^CGT
NheI 1777 GCTAGC G^CTAGC
NspI 1784 GCATGC [GA]CATG^[CT]
PshAI 785 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 2978 CTGCAG CTGCA^G
RcaI 3419 TCATGA T^CATGA
SapI 1726 GAAGAGC GCTCTTC(1/4)
SphI 1784 GCATGC GCATG^C
StuI 1740 AGGCCT AGG^CCT
TaqI 1863 TCGA T^CGA
TthHB8I 1863 TCGA T^CGA
XcmI 1402 CCAACAAACAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 1768 GTAGAC GT^[AC][GT]AC
2818 GTATAC
AhdI 454 GACCGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2499 GACTATTAGTC
AocI 371 CCTCAGG CC^T[ACGT]AGG
3030 CCTGAGG
AspEI 454 GACCGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2499 GACTATTAGTC
AspHI 817 GAGCAC G[AT]GC[AT]^C
3088 GAGCAC
BcgI 2481 GCATGGCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3456 GCACTGGCTTCG
BcnI 1585 CCGGG CC^[GC]GG
3443 CCCGG
BglII 205 AGATCT A^GATCT
3426 AGATCT
BsiHKAI 817 GAGCAC G[AT]GC[AT]^C
3088 GAGCAC
BsmBI 2548 GAGACG CGTCTC(1/5)
2914 GAGACG
BssSI 2244 CACGAG CACGAG(-5/-1)
1733 CTCGTG
BstAPI 2962 GCACCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3447 GCAGGGACTGC
Bsu36I 371 CCTCAGG CC^T[ACGT]AGG
3030 CCTGAGG
Eam1105I 454 GACCGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2499 GACTATTAGTC
Eco81I 371 CCTCAGG CC^T[ACGT]AGG
3030 CCTGAGG
HgaI 76 GACGC GACGC(5/10)
969 GACGC
HindIII 217 AAGCTT A^AGCTT
2713 AAGCTT
NciI 1585 CCGGG CC^[GC]GG
3443 CCCGG
PpuMI 333 AGGACCC [GA]G^G[AT]CC[CT]
641 GGGACCC
PvuII 1718 CAGCTG CAG^CTG
2975 CAGCTG
ScaI 2997 AGTACT AGT^ACT
3068 AGTACT
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AvaI AvaII
BanI BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRI EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
NsiI PflMI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XhoII XmnI