(Circular) (Six-base) MAPSORT of: pFN21AE9394.seq Check: 7784 from: 1 to: 8194 pFN21AE9394 8194 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6004 278 Size: 53 83 186 936 4468 2468 Fragments arranged by size: 4468 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3774 4824 5537 1937 Size: 1837 1050 713 4594 Fragments arranged by size: 4594 1837 1050 713 Acc65I G'GTAC_C Cuts at: 4137 5515 4137 Size: 1378 6816 AclI AA'CG_TT Cuts at: 1563 6321 6694 1563 Size: 4758 373 3063 Fragments arranged by size: 4758 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2885 3459 3750 3770 5072 5090 5120 5232 Size: 1253 574 291 20 1302 18 30 112 Cuts at: 5232 6257 7641 7909 1632 Size: 1025 1384 268 1917 Fragments arranged by size: 1917 1384 1302 1253 1025 574 291 268 112 30 20 18 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7997 AflIII A'CryG_T Cuts at: 1203 7093 8070 1203 Size: 5890 977 1327 Fragments arranged by size: 5890 1327 977 AgeI A'CCGG_T Cuts at: 7025 7025 Size: 8194 AhdI GACnn_n'nnGTC Cuts at: 2464 4509 6923 2464 Size: 2045 2414 3735 Fragments arranged by size: 3735 2414 2045 AleI CACnn'nnGTG Cuts at: 1166 2460 1166 Size: 1294 6900 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4434 1184 Size: 3250 4944 AlwNI CAG_nnn'CTG Cuts at: 2933 3728 5458 5937 7509 2933 Size: 795 1730 479 1572 3618 Fragments arranged by size: 3618 1730 1572 795 479 ApaI G_GGCC'C Cuts at: 5402 5402 Size: 8194 ApaLI G'TGCA_C Cuts at: 6251 7407 6251 Size: 1156 7038 ApoI r'AATT_y Cuts at: 1493 2064 2281 3035 4366 5104 5328 5369 5503 Size: 571 217 754 1331 738 224 41 134 Cuts at: 5503 5729 5881 7990 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1331 754 738 571 226 224 217 152 134 41 AseI AT'TA_AT Cuts at: 160 1032 6748 160 Size: 872 5716 1606 Fragments arranged by size: 5716 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8194 AvaI C'yCGr_G Cuts at: 1870 1943 2643 3389 4010 5519 1870 Size: 73 700 746 621 1509 4545 Fragments arranged by size: 4545 1509 746 700 621 73 BamHI G'GATC_C Cuts at: 5524 5891 5524 Size: 367 7827 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3123 4137 4966 5515 6970 Size: 325 217 627 1336 1014 829 549 1455 Cuts at: 6970 618 Size: 1842 Fragments arranged by size: 1842 1455 1336 1014 829 627 549 325 217 BanII G_rGCy'C Cuts at: 1391 5402 5513 1391 Size: 4011 111 4072 Fragments arranged by size: 4072 4011 111 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2022 2085 3180 3852 4509 4607 4619 Size: 971 123 63 1095 672 657 98 12 Cuts at: 4619 928 Size: 4503 Fragments arranged by size: 4503 1095 971 672 657 123 98 63 12 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4475 4509 5450 5484 6385 6419 1903 Size: 34 2538 34 941 34 901 34 3678 Fragments arranged by size: 3678 2538 941 901 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6086 7296 6086 Size: 1210 6984 BclI T'GATC_A Cuts at: 1548 2605 1548 Size: 1057 7137 BfrBI ATG'CAT Cuts at: 2924 4796 4868 2924 Size: 1872 72 6250 Fragments arranged by size: 6250 1872 72 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6805 136 Size: 107 122 71 631 605 5133 1525 Fragments arranged by size: 5133 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2210 5431 8189 2210 Size: 3221 2758 2215 Fragments arranged by size: 3221 2758 2215 BlpI GC'TnA_GC Cuts at: 5611 5611 Size: 8194 Bme1580I G_kGCm'C Cuts at: 1790 3126 3536 5402 6255 7411 1790 Size: 1336 410 1866 853 1156 2573 Fragments arranged by size: 2573 1866 1336 1156 853 410 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8194 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2671 3546 5925 Size: 416 53 83 399 24 1254 875 2379 Cuts at: 5925 6883 442 Size: 958 1753 Fragments arranged by size: 2379 1753 1254 958 875 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3786 1056 Size: 2730 5464 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3402 3999 4275 4507 3402 Size: 597 276 232 7089 Fragments arranged by size: 7089 597 276 232 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2316 3102 3259 3781 6854 1239 Size: 1077 786 157 522 3073 2579 Fragments arranged by size: 3073 2579 1077 786 522 157 Bpu10I CC'TnA_GC Cuts at: 4884 4884 Size: 8194 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3486 3811 5106 6319 7184 7482 3486 Size: 325 1295 1213 865 298 4198 Fragments arranged by size: 4198 1295 1213 865 325 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2598 6857 882 Size: 617 1099 4259 2219 Fragments arranged by size: 4259 2219 1099 617 BsaAI yAC'GTr Cuts at: 493 1204 5985 493 Size: 711 4781 2702 Fragments arranged by size: 4781 2702 711 BsaBI GATnn'nnATC Cuts at: 8188 8188 Size: 8194 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2082 6001 6383 7018 Size: 53 83 186 936 549 3919 382 635 Cuts at: 7018 275 Size: 1451 Fragments arranged by size: 3919 1451 936 635 549 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2019 6627 7025 7299 7446 7857 2019 Size: 4608 398 274 147 411 2356 Fragments arranged by size: 4608 2356 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3842 3872 4565 4595 1178 Size: 30 165 30 2439 30 693 30 4777 Fragments arranged by size: 4777 2439 693 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2091 3229 4030 4651 1186 Size: 905 1138 801 621 4729 Fragments arranged by size: 4729 1138 905 801 621 BseYI C'CCAG_C Cuts at: 2348 2870 4451 7397 7893 2348 Size: 522 1581 2946 496 2649 Fragments arranged by size: 2946 2649 1581 522 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3639 4665 5444 1163 Size: 573 1903 1026 779 3913 Fragments arranged by size: 3913 1903 1026 779 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2461 5644 6405 7433 1540 Size: 275 186 460 3183 761 1028 2301 Fragments arranged by size: 3183 2301 1028 761 460 275 186 BsiHKAI G_wGCw'C Cuts at: 2826 5097 6255 6340 7411 2826 Size: 2271 1158 85 1071 3609 Fragments arranged by size: 3609 2271 1158 1071 85 BsmI GAATG_Cn' Cuts at: 2977 4870 4885 5705 5798 2977 Size: 1893 15 820 93 5373 Fragments arranged by size: 5373 1893 820 93 15 BsmBI CGTCTCn'nnnn_ Cuts at: 4547 4913 4547 Size: 366 7828 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2826 3126 3536 5097 5402 5513 6255 Size: 399 1036 300 410 1561 305 111 742 Cuts at: 6255 6340 7411 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1561 1071 1036 742 410 399 305 300 111 85 BspEI T'CCGG_A Cuts at: 7857 7857 Size: 8194 BspHI T'CATG_A Cuts at: 5424 6083 7813 5424 Size: 659 1730 5805 Fragments arranged by size: 5805 1730 659 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2408 5549 844 Size: 1564 3141 3489 Fragments arranged by size: 3489 3141 1564 BsrBI CCG'CTC Cuts at: 1394 1823 6081 1394 Size: 429 4258 3507 Fragments arranged by size: 4258 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6689 6863 65 Size: 1183 5441 174 1396 Fragments arranged by size: 5441 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6838 7025 1700 Size: 110 5028 187 2869 Fragments arranged by size: 5028 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8194 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8194 BssSI C'ACGA_G Cuts at: 3738 4249 6254 7266 3738 Size: 511 2005 1012 4666 Fragments arranged by size: 4666 2005 1012 511 BstAPI GCAn_nnn'nTGC Cuts at: 794 4973 5458 794 Size: 4179 485 3530 Fragments arranged by size: 4179 3530 485 BstBI TT'CG_AA Cuts at: 6998 6998 Size: 8194 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8194 BstXI CCAn_nnnn'nTGG Cuts at: 2843 2843 Size: 8194 BstYI r'GATC_y Cuts at: 1972 2210 3302 3320 4471 4927 5268 5431 5524 Size: 238 1092 18 1151 456 341 163 93 Cuts at: 5524 5891 6276 6293 7745 7831 7843 8189 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1151 1092 456 385 367 346 341 238 163 93 86 18 17 12 BstZ17I GTA'TAC Cuts at: 4825 4825 Size: 8194 Bsu36I CC'TnA_GG Cuts at: 2377 5036 2377 Size: 2659 5535 BtgI C'CryG_G Cuts at: 513 1065 2781 2799 2842 513 Size: 552 1716 18 43 5865 Fragments arranged by size: 5865 1716 552 43 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5029 5115 487 Size: 683 56 3803 86 3566 Fragments arranged by size: 3803 3566 683 86 56 BtsI GCAGTG_nn' Cuts at: 2763 3175 5710 6504 6524 2763 Size: 412 2535 794 20 4433 Fragments arranged by size: 4433 2535 794 412 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8194 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2709 2744 529 Size: 35 2145 35 5979 Fragments arranged by size: 5979 2145 35 35 DraI TTT'AAA Cuts at: 5498 5498 Size: 8194 DraIII CAC_nnn'GTG Cuts at: 3662 3662 Size: 8194 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7201 809 Size: 507 5885 1802 Fragments arranged by size: 5885 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5641 6530 8159 Size: 54 1425 50 275 18 3811 889 1629 Cuts at: 8159 8 Size: 43 Fragments arranged by size: 3811 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5641 1537 Size: 275 3829 4090 Fragments arranged by size: 4090 3829 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3478 3726 4089 4493 6124 1353 Size: 2125 248 363 404 1631 3423 Fragments arranged by size: 3423 2125 1631 404 363 248 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2068 6781 7155 7301 387 Size: 1064 138 479 4713 374 146 1280 Fragments arranged by size: 4713 1280 1064 479 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2316 2885 3102 3259 3459 3750 3770 Size: 393 684 569 217 157 200 291 20 Cuts at: 3770 3781 5072 5090 5120 5232 6257 6854 7641 Size: 11 1291 18 30 112 1025 597 787 Cuts at: 7641 7909 1239 Size: 268 1524 Fragments arranged by size: 1524 1291 1025 787 684 597 569 393 291 268 217 200 157 112 30 20 18 11 EcoNI CCTnn'n_nnAGG Cuts at: 3741 3741 Size: 8194 EcoO109I rG'GnC_Cy Cuts at: 2339 2647 5399 2339 Size: 308 2752 5134 Fragments arranged by size: 5134 2752 308 EcoRI G'AATT_C Cuts at: 4366 5104 5369 5503 5881 4366 Size: 738 265 134 378 6679 Fragments arranged by size: 6679 738 378 265 134 EcoRV GAT'ATC Cuts at: 5490 5490 Size: 8194 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3927 4741 4978 5262 3927 Size: 814 237 284 6859 Fragments arranged by size: 6859 814 284 237 FspI TGC'GCA Cuts at: 6700 6700 Size: 8194 HaeII r_GCGC'y Cuts at: 1678 7341 1678 Size: 5663 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2132 2164 2255 2287 2450 Size: 32 135 32 560 32 91 32 163 Cuts at: 2450 2482 3842 3874 3935 3967 4495 4527 4784 Size: 32 1360 32 61 32 528 32 257 Cuts at: 4784 4816 4912 4944 6835 6867 6909 6941 1373 Size: 32 96 32 1891 32 42 32 2626 Fragments arranged by size: 2626 1891 1360 560 528 257 163 135 96 91 61 42 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3900 5538 5784 669 Size: 988 281 1962 1638 246 3079 Fragments arranged by size: 3079 1962 1638 988 281 246 HindIII A'AGCT_T Cuts at: 748 2222 4718 748 Size: 1474 2496 4224 Fragments arranged by size: 4224 2496 1474 HpaI GTT'AAC Cuts at: 5784 5784 Size: 8194 KpnI G_GTAC'C Cuts at: 4141 5519 4141 Size: 1378 6816 MfeI C'AATT_G Cuts at: 5793 5793 Size: 8194 MluI A'CGCG_T Cuts at: 8070 8070 Size: 8194 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3071 4311 5331 7308 7492 1305 Size: 1766 1240 1020 1977 184 2007 Fragments arranged by size: 2007 1977 1766 1240 1020 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6372 Fragments arranged by size: 6372 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2460 2601 3255 4311 6152 6511 6670 Size: 648 1294 141 654 1056 1841 359 159 Cuts at: 6670 518 Size: 2042 Fragments arranged by size: 2042 1841 1294 1056 654 648 359 159 141 MspA1I CmG'CkG Cuts at: 1606 2407 3725 4451 4982 5610 6287 7435 7680 Size: 801 1318 726 531 628 677 1148 245 Cuts at: 7680 1606 Size: 2120 Fragments arranged by size: 2120 1318 1148 801 726 677 628 531 245 NcoI C'CATG_G Cuts at: 513 1065 2781 2799 2842 513 Size: 552 1716 18 43 5865 Fragments arranged by size: 5865 1716 552 43 18 NdeI CA'TA_TG Cuts at: 387 387 Size: 8194 NheI G'CTAG_C Cuts at: 1052 3782 1052 Size: 2730 5464 NotI GC'GGCC_GC Cuts at: 5641 5641 Size: 8194 NsiI A_TGCA'T Cuts at: 2926 4798 4870 2926 Size: 1872 72 6250 Fragments arranged by size: 6250 1872 72 NspI r_CATG'y Cuts at: 1132 1229 3793 5552 7097 1132 Size: 97 2564 1759 1545 2229 Fragments arranged by size: 2564 2229 1759 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8194 PciI A'CATG_T Cuts at: 7093 7093 Size: 8194 PflMI CCAn_nnn'nTGG Cuts at: 1108 2917 5135 5278 1108 Size: 1809 2218 143 4024 Fragments arranged by size: 4024 2218 1809 143 PfoI T'CCnGG_A Cuts at: 3589 3589 Size: 8194 PmeI GTTT'AAAC Cuts at: 5498 5498 Size: 8194 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6263 6295 7776 7808 6263 Size: 32 1481 32 6649 Fragments arranged by size: 6649 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2339 2647 2339 Size: 308 7886 PshAI GACnn'nnGTC Cuts at: 1613 2794 1613 Size: 1181 7013 PsiI TTA'TAA Cuts at: 5112 5764 5112 Size: 652 7542 PspOMI G'GGCC_C Cuts at: 5398 5398 Size: 8194 PstI C_TGCA'G Cuts at: 830 4987 5546 830 Size: 4157 559 3478 Fragments arranged by size: 4157 3478 559 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8194 PvuII CAG'CTG Cuts at: 3725 4982 3725 Size: 1257 6937 SalI G'TCGA_C Cuts at: 1936 5536 1936 Size: 3600 4594 SanDI GG'GwC_CC Cuts at: 2647 2647 Size: 8194 SapI GCTCTTCn'nnn_ Cuts at: 3726 3726 Size: 8194 SbfI CC_TGCA'GG Cuts at: 5546 5546 Size: 8194 ScaI AGT'ACT Cuts at: 5004 5075 6442 8154 5004 Size: 71 1367 1712 5044 Fragments arranged by size: 5044 1712 1367 71 SfcI C'TryA_G Cuts at: 826 1045 2244 2827 3687 3771 3873 4977 4983 Size: 219 1199 583 860 84 102 1104 6 Cuts at: 4983 5542 6677 7358 7549 826 Size: 559 1135 681 191 1471 Fragments arranged by size: 1471 1199 1135 1104 860 681 583 559 219 191 102 84 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8194 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8194 SmaI CCC'GGG Cuts at: 1872 5521 1872 Size: 3649 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3501 3826 5121 6298 7199 7461 Size: 197 926 1558 325 1295 1177 901 262 Cuts at: 7461 820 Size: 1553 Fragments arranged by size: 1558 1553 1295 1177 926 901 325 262 197 SnaBI TAC'GTA Cuts at: 493 5985 493 Size: 5492 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8194 SphI G_CATG'C Cuts at: 1132 3793 5552 1132 Size: 2661 1759 3774 Fragments arranged by size: 3774 2661 1759 SspI AAT'ATT Cuts at: 5 52 6118 5 Size: 47 6066 2081 Fragments arranged by size: 6066 2081 47 StuI AGG'CCT Cuts at: 1517 3747 1517 Size: 2230 5964 StyI C'CwwG_G Cuts at: 513 1065 2781 2799 2842 4215 4629 5633 513 Size: 552 1716 18 43 1373 414 1004 3074 Fragments arranged by size: 3074 1716 1373 1004 552 414 43 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2362 4619 6221 6238 6391 6576 7021 Size: 1255 782 2257 1602 17 153 185 445 Cuts at: 7021 325 Size: 1498 Fragments arranged by size: 2257 1602 1498 1255 782 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3218 5002 5073 Size: 274 80 33 51 1443 1241 1784 71 Cuts at: 5073 6440 8152 96 Size: 1367 1712 138 Fragments arranged by size: 1784 1712 1443 1367 1241 274 138 80 71 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5253 5285 8154 8186 5253 Size: 32 2869 32 5261 Fragments arranged by size: 5261 2869 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8194 XbaI T'CTAG_A Cuts at: 5530 5530 Size: 8194 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3414 3414 Size: 8194 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8194 XmaI C'CCGG_G Cuts at: 1870 5519 1870 Size: 3649 4545 XmnI GAAnn'nnTTC Cuts at: 2195 4741 5209 6323 2195 Size: 2546 468 1114 4066 Fragments arranged by size: 4066 2546 1114 468 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6002 276 Size: 53 83 186 936 4468 2468 Fragments arranged by size: 4468 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BsiWI EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI