################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BbrPI BbvCI BcgI BclI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MamI MfeI MluI MluNI MroI MscI MspA1I MunI NciI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I Tth111I Van91I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AcyI 78 GGCGCC G[GA]^CG[CT]C AlwNI 361 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 598 GGGCCC GGGCC^C AvaI 98 CCCGAG C^[CT]CG[GA]G AvaII 69 GGACC G^G[AT]CC BamHI 620 GGATCC G^GATCC BanI 78 GGCGCC G^G[CT][GA]CC BbeI 78 GGCGCC GGCGC^C BciVI 668 GGATAC GTATCC(6/5) BfaI 464 CTAG C^TAG BpmI 739 CTGGAG CTGGAG(16/14) BsaHI 78 GGCGCC G[GA]^CG[CT]C BsgI 848 GTGCAG GTGCAG(16/14) BsmAI 309 GAGAC GTCTC(1/5) BsmI 436 GAATGC GAATGC(1/-1) BsoBI 98 CCCGAG C^[CT]CG[GA]G BsrI 220 ACTGG ACTGG(1/-1) BssHII 24 GCGCGC G^CGCGC BstF5I 145 GGATG GGATG(2/0) DsaI 614 CCATGG C^C[GA][CT]GG EaeI 637 CGGCCA [CT]^GGCC[GA] EcoNI 588 CCTCTCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 614 CCATGG C^C[AT][AT]GG FokI 145 GGATG GGATG(9/13) HgaI 4 GACGC GACGC(5/10) Hin1I 78 GGCGCC G[GA]^CG[CT]C HphI 716 TCACC GGTGA(8/7) KasI 78 GGCGCC G^GCGCC MaeI 464 CTAG C^TAG MaeII 547 ACGT A^CGT MseI 448 TTAA T^TAA NaeI 635 GCCGGC GCC^GGC NarI 78 GGCGCC GG^CGCC NcoI 614 CCATGG C^CATGG NgoMIV 635 GCCGGC G^CCGGC PleI 101 GAGTC GAGTC(4/5) PpuMI 68 AGGACCC [GA]G^G[AT]CC[CT] SfcI 304 CTACAG C^T[GA][CT]AG SfoI 78 GGCGCC GGC^GCC SgrAI 74 CACCGGCG C[GA]^CCGG[CT]G SmlI 559 CTCAAG C^T[CT][GA]AG StyI 614 CCATGG C^C[AT][AT]GG TaqI 812 TCGA T^CGA Tru9I 448 TTAA T^TAA TspRI 270 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 812 TCGA T^CGA XbaI 463 TCTAGA T^CTAGA XcmI 73 CCACCGGCGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccII 25 CGCG CG^CG 45 CGCG BbsI 256 GAAGAC GAAGAC(2/6) 153 GTCTTC BbvI 626 GCAGC GCAGC(8/12) 817 GCTGC BglII 162 AGATCT A^GATCT 483 AGATCT BpuAI 256 GAAGAC GAAGAC(2/6) 153 GTCTTC BsmFI 96 GTCCC GGGAC(10/14) 724 GTCCC BsrFI 75 ACCGGC [GA]^CCGG[CT] 635 GCCGGC BstUI 25 CGCG CG^CG 45 CGCG Cfr10I 75 ACCGGC [GA]^CCGG[CT] 635 GCCGGC EarI 88 GAAGAG CTCTTC(1/4) 472 GAAGAG HaeII 78 GGCGCC [GA]GCGC^[CT] 628 AGCGCC HapII 76 CCGG C^CGG 636 CCGG HpaII 76 CCGG C^CGG 636 CCGG Ksp632I 88 GAAGAG CTCTTC(1/4) 472 GAAGAG MaeIII 340 GTTAC ^GT[ACGT]AC 403 GTTAC MslI 270 CACTGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 417 CATAATAATG MspI 76 CCGG C^CGG 636 CCGG MvnI 25 CGCG CG^CG 45 CGCG SfaNI 577 GATGC GCATC(5/9) 224 GCATC TseI 626 GCAGC G^C[AT]GC 817 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI BanII BmyI BsaJI BseRI BsiYI BslI Bsp1286I BssKI BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI HaeIII HhaI HinP1I HinfI ItaI MboI MboII MflI MnlI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI TfiI Tsp509I XhoII