(Circular) (Six-base) MAPSORT of: pFN21AE9676.seq Check: 5633 from: 1 to: 5569 pFN21AE9676 5569 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3379 278 Size: 53 83 186 936 1843 2468 Fragments arranged by size: 2468 1843 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2912 1937 Size: 975 4594 Acc65I G'GTAC_C Cuts at: 2890 2890 Size: 5569 AclI AA'CG_TT Cuts at: 1563 2868 3696 4069 1563 Size: 1305 828 373 3063 Fragments arranged by size: 3063 1305 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3632 5016 5284 1632 Size: 2000 1384 268 1917 Fragments arranged by size: 2000 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5372 AflIII A'CryG_T Cuts at: 1203 4468 5445 1203 Size: 3265 977 1327 Fragments arranged by size: 3265 1327 977 AgeI A'CCGG_T Cuts at: 4400 4400 Size: 5569 AhdI GACnn_n'nnGTC Cuts at: 4298 4298 Size: 5569 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5569 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5569 AlwNI CAG_nnn'CTG Cuts at: 2371 3312 4884 2371 Size: 941 1572 3056 Fragments arranged by size: 3056 1572 941 ApaI G_GGCC'C Cuts at: 2607 2607 Size: 5569 ApaLI G'TGCA_C Cuts at: 3626 4782 3626 Size: 1156 4413 ApoI r'AATT_y Cuts at: 1493 2360 2376 2459 2878 3104 3256 5365 1493 Size: 867 16 83 419 226 152 2109 1697 Fragments arranged by size: 2109 1697 867 419 226 152 83 16 AseI AT'TA_AT Cuts at: 160 1032 4123 160 Size: 872 3091 1606 Fragments arranged by size: 3091 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5569 AvaI C'yCGr_G Cuts at: 1870 1943 2103 2894 1870 Size: 73 160 791 4545 Fragments arranged by size: 4545 791 160 73 BamHI G'GATC_C Cuts at: 2625 2899 3266 2625 Size: 274 367 4928 Fragments arranged by size: 4928 367 274 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2083 2890 4345 618 Size: 325 217 627 296 807 1455 1842 Fragments arranged by size: 1842 1455 807 627 325 296 217 BanII G_rGCy'C Cuts at: 1391 2436 2607 2743 2888 1391 Size: 1045 171 136 145 4072 Fragments arranged by size: 4072 1045 171 145 136 BbeI G_GCGC'C Cuts at: 2087 2087 Size: 5569 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2151 2268 928 Size: 971 252 117 4229 Fragments arranged by size: 4229 971 252 117 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3760 3794 1903 Size: 34 1823 34 3678 Fragments arranged by size: 3678 1823 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2667 3461 4671 2667 Size: 794 1210 3565 Fragments arranged by size: 3565 1210 794 BclI T'GATC_A Cuts at: 1548 1548 Size: 5569 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4180 136 Size: 107 122 71 631 605 2508 1525 Fragments arranged by size: 2508 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2167 2488 5564 2167 Size: 321 3076 2172 Fragments arranged by size: 3076 2172 321 BlpI GC'TnA_GC Cuts at: 2986 2986 Size: 5569 Bme1580I G_kGCm'C Cuts at: 1790 2607 3630 4786 1790 Size: 817 1023 1156 2573 Fragments arranged by size: 2573 1156 1023 817 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5569 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3300 4258 442 Size: 416 53 83 399 24 1883 958 1753 Fragments arranged by size: 1883 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5569 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2623 2623 Size: 5569 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2765 4229 1239 Size: 1526 1464 2579 Fragments arranged by size: 2579 1526 1464 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2549 3694 4559 4857 2549 Size: 1145 865 298 3261 Fragments arranged by size: 3261 1145 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4232 882 Size: 617 2733 2219 Fragments arranged by size: 2733 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3360 493 Size: 711 2156 2702 Fragments arranged by size: 2702 2156 711 BsaBI GATnn'nnATC Cuts at: 5563 5563 Size: 5569 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2084 3376 3758 4393 Size: 53 83 186 936 551 1292 382 635 Cuts at: 4393 275 Size: 1451 Fragments arranged by size: 1451 1292 936 635 551 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4002 4400 4674 4821 5232 4002 Size: 398 274 147 411 4339 Fragments arranged by size: 4339 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5344 Fragments arranged by size: 5344 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2599 2769 2873 1186 Size: 1413 170 104 3882 Fragments arranged by size: 3882 1413 170 104 BseYI C'CCAG_C Cuts at: 4772 5268 4772 Size: 496 5073 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2874 1163 Size: 573 1138 3858 Fragments arranged by size: 3858 1138 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3019 3780 4808 1540 Size: 275 186 1018 761 1028 2301 Fragments arranged by size: 2301 1028 1018 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3630 3715 4786 3630 Size: 85 1071 4413 Fragments arranged by size: 4413 1071 85 BsmI GAATG_Cn' Cuts at: 2447 3080 3173 2447 Size: 633 93 4843 Fragments arranged by size: 4843 633 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2436 2607 2743 2888 3630 3715 4786 Size: 399 646 171 136 145 742 85 1071 Cuts at: 4786 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 646 399 171 145 136 85 BspEI T'CCGG_A Cuts at: 5232 5232 Size: 5569 BspHI T'CATG_A Cuts at: 3458 5188 3458 Size: 1730 3839 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2924 844 Size: 2080 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3456 1394 Size: 429 1633 3507 Fragments arranged by size: 3507 1633 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4064 4238 65 Size: 1183 2816 174 1396 Fragments arranged by size: 2816 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2080 2640 4213 4400 1700 Size: 110 270 560 1573 187 2869 Fragments arranged by size: 2869 1573 560 270 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5569 BssHII G'CGCG_C Cuts at: 1925 2029 1925 Size: 104 5465 BssSI C'ACGA_G Cuts at: 3629 4641 3629 Size: 1012 4557 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5569 BstBI TT'CG_AA Cuts at: 4373 4373 Size: 5569 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5569 BstYI r'GATC_y Cuts at: 1972 2167 2488 2625 2713 2899 3266 3651 3668 Size: 195 321 137 88 186 367 385 17 Cuts at: 3668 5120 5206 5218 5564 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 321 195 186 137 88 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2619 513 Size: 552 939 615 3463 Fragments arranged by size: 3463 939 615 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4830 Fragments arranged by size: 4830 683 56 BtsI GCAGTG_nn' Cuts at: 3085 3879 3899 3085 Size: 794 20 4755 Fragments arranged by size: 4755 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5569 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5534 DraI TTT'AAA Cuts at: 2873 2873 Size: 5569 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4576 809 Size: 507 3260 1802 Fragments arranged by size: 3260 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2642 3016 3905 Size: 54 1425 50 275 18 812 374 889 Cuts at: 3905 5534 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 812 374 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3016 1537 Size: 275 1204 4090 Fragments arranged by size: 4090 1204 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2088 2472 3499 1353 Size: 735 384 1027 3423 Fragments arranged by size: 3423 1027 735 384 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4156 4530 4676 387 Size: 1064 138 2567 374 146 1280 Fragments arranged by size: 2567 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2765 3632 4229 5016 5284 1239 Size: 393 1133 867 597 787 268 1524 Fragments arranged by size: 1524 1133 867 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2597 2597 Size: 5569 EcoO109I rG'GnC_Cy Cuts at: 2074 2603 2604 2074 Size: 529 1 5039 Fragments arranged by size: 5039 529 1 EcoRI G'AATT_C Cuts at: 2878 3256 2878 Size: 378 5191 FspI TGC'GCA Cuts at: 4075 4075 Size: 5569 HaeII r_GCGC'y Cuts at: 1678 2087 2637 4716 1678 Size: 409 550 2079 2531 Fragments arranged by size: 2531 2079 550 409 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2381 2413 2473 2505 4210 Size: 32 135 32 809 32 60 32 1705 Cuts at: 4210 4242 4284 4316 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1705 809 135 60 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2913 3159 669 Size: 988 281 975 246 3079 Fragments arranged by size: 3079 988 975 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5569 HpaI GTT'AAC Cuts at: 3159 3159 Size: 5569 KasI G'GCGC_C Cuts at: 2083 2083 Size: 5569 KpnI G_GTAC'C Cuts at: 2894 2894 Size: 5569 MfeI C'AATT_G Cuts at: 3168 3168 Size: 5569 MluI A'CGCG_T Cuts at: 5445 5445 Size: 5569 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4683 4867 1305 Size: 3378 184 2007 Fragments arranged by size: 3378 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3747 Fragments arranged by size: 3747 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2279 2426 3527 3886 4045 518 Size: 648 1113 147 1101 359 159 2042 Fragments arranged by size: 2042 1113 1101 648 359 159 147 MspA1I CmG'CkG Cuts at: 1606 2985 3662 4810 5055 1606 Size: 1379 677 1148 245 2120 Fragments arranged by size: 2120 1379 1148 677 245 NaeI GCC'GGC Cuts at: 2642 2642 Size: 5569 NarI GG'CG_CC Cuts at: 2084 2084 Size: 5569 NcoI C'CATG_G Cuts at: 513 1065 2004 2619 513 Size: 552 939 615 3463 Fragments arranged by size: 3463 939 615 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5569 NgoMIV G'CCGG_C Cuts at: 2640 2640 Size: 5569 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5569 NotI GC'GGCC_GC Cuts at: 3016 3016 Size: 5569 NspI r_CATG'y Cuts at: 1132 1229 2927 4472 1132 Size: 97 1698 1545 2229 Fragments arranged by size: 2229 1698 1545 97 PasI CC'CwG_GG Cuts at: 1348 2088 1348 Size: 740 4829 PciI A'CATG_T Cuts at: 4468 4468 Size: 5569 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5569 PmeI GTTT'AAAC Cuts at: 2873 2873 Size: 5569 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3638 3670 5151 5183 3638 Size: 32 1481 32 4024 Fragments arranged by size: 4024 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2074 2074 Size: 5569 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5569 PsiI TTA'TAA Cuts at: 3139 3139 Size: 5569 PspOMI G'GGCC_C Cuts at: 2603 2603 Size: 5569 PstI C_TGCA'G Cuts at: 830 2921 830 Size: 2091 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5569 SalI G'TCGA_C Cuts at: 1936 2911 1936 Size: 975 4594 SbfI CC_TGCA'GG Cuts at: 2921 2921 Size: 5569 ScaI AGT'ACT Cuts at: 3817 5529 3817 Size: 1712 3857 SfcI C'TryA_G Cuts at: 826 1045 2309 2917 4052 4733 4924 826 Size: 219 1264 608 1135 681 191 1471 Fragments arranged by size: 1471 1264 1135 681 608 219 191 SfoI GGC'GCC Cuts at: 2085 2085 Size: 5569 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5569 SgrAI Cr'CCGG_yG Cuts at: 1700 2080 1700 Size: 380 5189 SmaI CCC'GGG Cuts at: 1872 2896 1872 Size: 1024 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2564 3673 4574 4836 820 Size: 197 926 621 1109 901 262 1553 Fragments arranged by size: 1553 1109 926 901 621 262 197 SnaBI TAC'GTA Cuts at: 493 3360 493 Size: 2867 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5569 SphI G_CATG'C Cuts at: 1132 2927 1132 Size: 1795 3774 SspI AAT'ATT Cuts at: 5 52 3493 5 Size: 47 3441 2081 Fragments arranged by size: 3441 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5569 StyI C'CwwG_G Cuts at: 513 1065 2004 2619 3008 513 Size: 552 939 615 389 3074 Fragments arranged by size: 3074 939 615 552 389 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3596 3613 3766 3951 4396 325 Size: 1255 2016 17 153 185 445 1498 Fragments arranged by size: 2016 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3815 5527 96 Size: 274 80 33 51 1443 1838 1712 138 Fragments arranged by size: 1838 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5529 5561 5529 Size: 32 5537 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5569 XbaI T'CTAG_A Cuts at: 2468 2905 2468 Size: 437 5132 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2085 2085 Size: 5569 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5569 XmaI C'CCGG_G Cuts at: 1870 2894 1870 Size: 1024 4545 XmnI GAAnn'nnTTC Cuts at: 3698 3698 Size: 5569 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3377 276 Size: 53 83 186 936 1843 2468 Fragments arranged by size: 2468 1843 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI