Restriction map for
pFN21AE9676
(Linear) (Six-base) MAPSORT of: KIEE9676.seq Check: 9363 from: 1 to: 864
KIEE9676.orf
With 182 enzymes: SgfI *
December 27, 2011 16:43 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 366 864
Size: 366 498
ApaI G_GGCC'C
Cuts at: 0 602 864
Size: 602 262
ApoI r'AATT_y
Cuts at: 0 355 371 454 864
Size: 355 16 83 410
Fragments arranged by size:
410 355 83 16
AvaI C'yCGr_G
Cuts at: 0 98 864
Size: 98 766
BamHI G'GATC_C
Cuts at: 0 620 864
Size: 620 244
BanI G'GyrC_C
Cuts at: 0 78 864
Size: 78 786
BanII G_rGCy'C
Cuts at: 0 431 602 738 864
Size: 431 171 136 126
Fragments arranged by size:
431 171 136 126
BbeI G_GCGC'C
Cuts at: 0 82 864
Size: 82 782
BbsI GAAGACnn'nnnn_
Cuts at: 0 146 263 864
Size: 146 117 601
Fragments arranged by size:
601 146 117
BciVI GTATCCnnnnn_n'
Cuts at: 0 662 864
Size: 662 202
BglII A'GATC_T
Cuts at: 0 162 483 864
Size: 162 321 381
Fragments arranged by size:
381 321 162
Bme1580I G_kGCm'C
Cuts at: 0 602 864
Size: 602 262
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 618 864
Size: 618 246
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 760 864
Size: 760 104
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 544 864
Size: 544 320
BsaHI Gr'CG_yC
Cuts at: 0 79 864
Size: 79 785
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 594 764 864
Size: 594 170 100
Fragments arranged by size:
594 170 100
BsmI GAATG_Cn'
Cuts at: 0 442 864
Size: 442 422
Bsp1286I G_dGCh'C
Cuts at: 0 431 602 738 864
Size: 431 171 136 126
Fragments arranged by size:
431 171 136 126
BsrFI r'CCGG_y
Cuts at: 0 75 635 864
Size: 75 560 229
Fragments arranged by size:
560 229 75
BssHII G'CGCG_C
Cuts at: 0 24 864
Size: 24 840
BstYI r'GATC_y
Cuts at: 0 162 483 620 708 864
Size: 162 321 137 88 156
Fragments arranged by size:
321 162 156 137 88
BtgI C'CryG_G
Cuts at: 0 614 864
Size: 614 250
EaeI y'GGCC_r
Cuts at: 0 637 864
Size: 637 227
EarI CTCTTCn'nnn_
Cuts at: 0 83 467 864
Size: 83 384 397
Fragments arranged by size:
397 384 83
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 760 864
Size: 760 104
EcoNI CCTnn'n_nnAGG
Cuts at: 0 592 864
Size: 592 272
EcoO109I rG'GnC_Cy
Cuts at: 0 69 598 599 864
Size: 69 529 1 265
Fragments arranged by size:
529 265 69 1
HaeII r_GCGC'y
Cuts at: 0 82 632 864
Size: 82 550 232
Fragments arranged by size:
550 232 82
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 376 408 468 500 864
Size: 376 32 60 32 364
Fragments arranged by size:
376 364 60 32 32
KasI G'GCGC_C
Cuts at: 0 78 864
Size: 78 786
MslI CAynn'nnrTG
Cuts at: 0 274 421 864
Size: 274 147 443
Fragments arranged by size:
443 274 147
NaeI GCC'GGC
Cuts at: 0 637 864
Size: 637 227
NarI GG'CG_CC
Cuts at: 0 79 864
Size: 79 785
NcoI C'CATG_G
Cuts at: 0 614 864
Size: 614 250
NgoMIV G'CCGG_C
Cuts at: 0 635 864
Size: 635 229
PasI CC'CwG_GG
Cuts at: 0 83 864
Size: 83 781
PpuMI rG'GwC_Cy
Cuts at: 0 69 864
Size: 69 795
PspOMI G'GGCC_C
Cuts at: 0 598 864
Size: 598 266
SfcI C'TryA_G
Cuts at: 0 304 864
Size: 304 560
SfoI GGC'GCC
Cuts at: 0 80 864
Size: 80 784
SgrAI Cr'CCGG_yG
Cuts at: 0 75 864
Size: 75 789
SmlI C'TyrA_G
Cuts at: 0 559 864
Size: 559 305
StyI C'CwwG_G
Cuts at: 0 614 864
Size: 614 250
XbaI T'CTAG_A
Cuts at: 0 463 864
Size: 463 401
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 80 864
Size: 80 784
Enzymes that do cut:
AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI
BbsI BciVI BglII Bme1580I BplI BpmI BpuEI BsaHI
BseRI BsmI Bsp1286I BsrFI BssHII BstYI BtgI EaeI
EarI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MslI
NaeI NarI NcoI NgoMIV PasI PpuMI PspOMI SfcI
SfoI SgrAI SmlI StyI XbaI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaLI AscI
AseI AsiSI AvrII BaeI BbvCI BcgI BclI BfrBI
BglI BlpI BmgBI BmrI BmtI Bpu10I BsaI BsaAI
BsaBI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MspA1I NdeI
NheI NotI NruI NsiI NspI PacI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TatI
TstI Tth111I XhoI XmaI XmnI ZraI