################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BamHI BbrPI BcgI BciVI BfrI BglI BglII BlnI BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI Bst1107I BstBI BstEII BstPI BstXI BstZ17I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoT22I FseI FspI HincII HindII HindIII HpaI KpnI KspI MamI MfeI MluI MluNI MroI MscI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AcsI 962 GAATTC [GA]^AATT[CT] AlwI 1110 GATCC GGATC(4/5) AocI 763 CCTAAGG CC^T[ACGT]AGG ApaI 759 GGGCCC GGGCC^C ApoI 962 GAATTC [GA]^AATT[CT] Asp700I 922 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbvCI 684 CCTCAGC CCTCAGC(-5/-2) BclI 1011 TGATCA T^GATCA BfaI 967 CTAG C^TAG BlpI 52 GCTAAGC GC^T[ACGT]AGC Bpu1102I 52 GCTAAGC GC^T[ACGT]AGC BsaI 325 GAGACC GGTCTC(1/5) BsmBI 1085 CGTCTC CGTCTC(1/5) BsrDI 1168 GCAATG GCAATG(2/0) BsrFI 357 GCCGGC [GA]^CCGG[CT] BssHII 30 GCGCGC G^CGCGC BssSI 691 CTCGTG CACGAG(-5/-1) BstAPI 975 GCACGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 1109 AGATCC [GA]^GATC[CT] Bsu36I 763 CCTAAGG CC^T[ACGT]AGG CelII 52 GCTAAGC GC^T[ACGT]AGC Cfr10I 357 GCCGGC [GA]^CCGG[CT] DrdI 829 GACAAGTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 47 CCGTGG C^C[GA][CT]GG EarI 913 GAAGAG CTCTTC(1/4) Eco81I 763 CCTAAGG CC^T[ACGT]AGG EcoNI 900 CCTCAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 962 GAATTC G^AATTC EcoRV 607 GATATC GAT^ATC FbaI 1011 TGATCA T^GATCA Ksp632I 913 GAAGAG CTCTTC(1/4) MaeI 967 CTAG C^TAG MflI 1109 AGATCC [GA]^GATC[CT] MslI 1249 CACTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 357 GCCGGC GCC^GGC NgoMIV 357 GCCGGC G^CCGGC PleI 1062 GAGTC GAGTC(4/5) PshAI 870 GACAGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 1251 CTGCAG CTGCA^G SapI 913 GAAGAGC GCTCTTC(1/4) StuI 704 AGGCCT AGG^CCT XhoII 1109 AGATCC [GA]^GATC[CT] XmnI 922 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AccII 31 CGCG CG^CG 125 CGCG AspHI 457 GAGCAC G[AT]GC[AT]^C 1138 GAGCAC AvaI 36 CCCGAG C^[CT]CG[GA]G 1214 CCCGAG BbeI 423 GGCGCC GGCGC^C 1223 GGCGCC BbsI 190 GAAGAC GAAGAC(2/6) 574 GAAGAC BcnI 58 CCGGG CC^[GC]GG 157 CCCGG BpmI 1120 CTCCAG CTGGAG(16/14) 1241 CTCCAG BpuAI 190 GAAGAC GAAGAC(2/6) 574 GAAGAC BseRI 349 GAGGAG GAGGAG(10/8) 772 GAGGAG BsiHKAI 457 GAGCAC G[AT]GC[AT]^C 1138 GAGCAC BsoBI 36 CCCGAG C^[CT]CG[GA]G 1214 CCCGAG BstF5I 1015 CATCC GGATG(2/0) 1155 GGATG BstUI 31 CGCG CG^CG 125 CGCG DpnII 1012 GATC ^GATC 1110 GATC EaeI 635 CGGCCA [CT]^GGCC[GA] 782 TGGCCG FauI 19 CCCGC CCCGC(4/6) 126 GCGGG FokI 1015 CATCC GGATG(9/13) 1155 GGATG HphI 493 GGTGA GGTGA(8/7) 1188 GGTGA KasI 423 GGCGCC G^GCGCC 1223 GGCGCC MaeIII 856 GTGAC ^GT[ACGT]AC 1256 GTGAC MboI 1012 GATC ^GATC 1110 GATC MspA1I 602 CAGCGG C[AC]G^C[GT]G 872 CAGCGG MvnI 31 CGCG CG^CG 125 CGCG NarI 423 GGCGCC GG^CGCC 1223 GGCGCC NciI 58 CCGGG CC^[GC]GG 157 CCCGG NdeII 1012 GATC ^GATC 1110 GATC PpuMI 14 AGGTCCC [GA]G^G[AT]CC[CT] 60 GGGTCCT Sau3AI 1012 GATC ^GATC 1110 GATC SfcI 864 CTATAG C^T[GA][CT]AG 1251 CTGCAG SfoI 423 GGCGCC GGC^GCC 1223 GGCGCC SmlI 335 CTCAAG C^T[CT][GA]AG 901 CTCAAG TaqI 521 TCGA T^CGA 611 TCGA TfiI 885 GATTC G^A[AT]TC 1029 GATTC Tsp45I 856 GTGAC ^GT[GC]AC 1256 GTGAC TthHB8I 521 TCGA T^CGA 611 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AciI AcyI AfaI AluI AvaII BanI BanII BbvI BmyI BsaHI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstNI Cac8I CfoI Cfr13I DdeI DraII EcoO109I EcoRII EcoT14I Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII ItaI MaeII MboII MnlI MspI MvaI MwoI NlaIII NlaIV RsaI Sau96I ScrFI SfaNI StyI TseI Tsp509I TspRI