(Circular) (Six-base) MAPSORT of: pFN21AB8296.seq Check: 1932 from: 1 to: 5971 pFN21AB8296 5971 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3781 278 Size: 53 83 186 936 2245 2468 Fragments arranged by size: 2468 2245 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3314 1937 Size: 1377 4594 Acc65I G'GTAC_C Cuts at: 3292 3292 Size: 5971 AclI AA'CG_TT Cuts at: 1563 4098 4471 1563 Size: 2535 373 3063 Fragments arranged by size: 3063 2535 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2388 2978 4034 5418 5686 1632 Size: 756 590 1056 1384 268 1917 Fragments arranged by size: 1917 1384 1056 756 590 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5774 AflIII A'CryG_T Cuts at: 1203 4870 5847 1203 Size: 3667 977 1327 Fragments arranged by size: 3667 1327 977 AgeI A'CCGG_T Cuts at: 4802 4802 Size: 5971 AhdI GACnn_n'nnGTC Cuts at: 4700 4700 Size: 5971 AleI CACnn'nnGTG Cuts at: 1166 3258 1166 Size: 2092 3879 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5971 AlwNI CAG_nnn'CTG Cuts at: 3714 5286 3714 Size: 1572 4399 ApaI G_GGCC'C Cuts at: 2768 2768 Size: 5971 ApaLI G'TGCA_C Cuts at: 4028 5184 4028 Size: 1156 4815 ApoI r'AATT_y Cuts at: 1493 2967 3280 3506 3658 5767 1493 Size: 1474 313 226 152 2109 1697 Fragments arranged by size: 2109 1697 1474 313 226 152 AseI AT'TA_AT Cuts at: 160 1032 4525 160 Size: 872 3493 1606 Fragments arranged by size: 3493 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 AvaI C'yCGr_G Cuts at: 1870 1943 2041 3219 3296 1870 Size: 73 98 1178 77 4545 Fragments arranged by size: 4545 1178 98 77 73 BamHI G'GATC_C Cuts at: 3301 3668 3301 Size: 367 5604 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2188 2428 3228 3292 4747 Size: 325 217 627 401 240 800 64 1455 Cuts at: 4747 618 Size: 1842 Fragments arranged by size: 1842 1455 800 627 401 325 240 217 64 BanII G_rGCy'C Cuts at: 1391 2396 2768 2859 3126 3290 1391 Size: 1005 372 91 267 164 4072 Fragments arranged by size: 4072 1005 372 267 164 91 BbeI G_GCGC'C Cuts at: 2432 3232 2432 Size: 800 5171 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2202 2586 928 Size: 971 303 384 4313 Fragments arranged by size: 4313 971 384 303 BbvCI CC'TCA_GC Cuts at: 2690 2690 Size: 5971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4162 4196 1903 Size: 34 2225 34 3678 Fragments arranged by size: 3678 2225 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3863 5073 3863 Size: 1210 4761 BclI T'GATC_A Cuts at: 1548 3016 1548 Size: 1468 4503 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4582 136 Size: 107 122 71 631 605 2910 1525 Fragments arranged by size: 2910 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5966 5966 Size: 5971 BlpI GC'TnA_GC Cuts at: 2058 3388 2058 Size: 1330 4641 Bme1580I G_kGCm'C Cuts at: 1790 2768 2982 4032 5188 1790 Size: 978 214 1050 1156 2573 Fragments arranged by size: 2573 1156 1050 978 214 BmgBI CAC'GTC Cuts at: 1318 2599 1318 Size: 1281 4690 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3702 4660 442 Size: 416 53 83 399 24 2285 958 1753 Fragments arranged by size: 2285 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5971 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3110 3231 4631 1239 Size: 1871 121 1400 2579 Fragments arranged by size: 2579 1871 1400 121 Bpu10I CC'TnA_GC Cuts at: 2547 2690 2734 2547 Size: 143 44 5784 Fragments arranged by size: 5784 143 44 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2325 2891 4096 4961 5259 2325 Size: 566 1205 865 298 3037 Fragments arranged by size: 3037 1205 865 566 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2324 4634 882 Size: 617 825 2310 2219 Fragments arranged by size: 2310 2219 825 617 BsaAI yAC'GTr Cuts at: 493 1204 3762 493 Size: 711 2558 2702 Fragments arranged by size: 2702 2558 711 BsaBI GATnn'nnATC Cuts at: 5965 5965 Size: 5971 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2429 3229 3267 3778 Size: 53 83 186 936 896 800 38 511 Cuts at: 3778 4160 4795 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 896 800 635 511 382 186 83 53 38 BsaWI w'CCGG_w Cuts at: 4404 4802 5076 5223 5634 4404 Size: 398 274 147 411 4741 Fragments arranged by size: 4741 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5746 Fragments arranged by size: 5746 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2369 2792 1186 Size: 1183 423 4365 Fragments arranged by size: 4365 1183 423 BseYI C'CCAG_C Cuts at: 2560 2605 5174 5670 2560 Size: 45 2569 496 2861 Fragments arranged by size: 2861 2569 496 45 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5398 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3421 4182 5210 1540 Size: 275 186 1420 761 1028 2301 Fragments arranged by size: 2301 1420 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2466 3147 4032 4117 5188 2466 Size: 681 885 85 1071 3249 Fragments arranged by size: 3249 1071 885 681 85 BsmI GAATG_Cn' Cuts at: 3482 3575 3482 Size: 93 5878 BsmBI CGTCTCn'nnnn_ Cuts at: 3096 3096 Size: 5971 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2396 2466 2768 2859 2982 3126 3147 Size: 399 606 70 302 91 123 144 21 Cuts at: 3147 3290 4032 4117 5188 1391 Size: 143 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 606 399 302 144 143 123 91 85 70 21 BspEI T'CCGG_A Cuts at: 5634 5634 Size: 5971 BspHI T'CATG_A Cuts at: 3860 5590 3860 Size: 1730 4241 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3326 844 Size: 2482 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3858 1394 Size: 429 2035 3507 Fragments arranged by size: 3507 2035 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3180 4466 4640 65 Size: 1183 1932 1286 174 1396 Fragments arranged by size: 1932 1396 1286 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2362 4615 4802 1700 Size: 110 552 2253 187 2869 Fragments arranged by size: 2869 2253 552 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5971 BssHII G'CGCG_C Cuts at: 1925 2035 1925 Size: 110 5861 BssSI C'ACGA_G Cuts at: 2696 4031 5043 2696 Size: 1335 1012 3624 Fragments arranged by size: 3624 1335 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2986 794 Size: 2192 3779 BstBI TT'CG_AA Cuts at: 4775 4775 Size: 5971 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5971 BstYI r'GATC_y Cuts at: 1972 3114 3301 3668 4053 4070 5522 5608 5620 Size: 1142 187 367 385 17 1452 86 12 Cuts at: 5620 5966 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1142 385 367 346 187 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2769 2769 Size: 5971 BtgI C'CryG_G Cuts at: 513 1065 2004 2052 513 Size: 552 939 48 4432 Fragments arranged by size: 4432 939 552 48 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5232 Fragments arranged by size: 5232 683 56 BtsI GCAGTG_nn' Cuts at: 3253 3265 3487 4281 4301 3253 Size: 12 222 794 20 4923 Fragments arranged by size: 4923 794 222 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5971 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2434 2469 529 Size: 35 1870 35 4031 Fragments arranged by size: 4031 1870 35 35 DraI TTT'AAA Cuts at: 3275 3275 Size: 5971 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2840 4978 809 Size: 507 1524 2138 1802 Fragments arranged by size: 2138 1802 1524 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2640 2787 3418 Size: 54 1425 50 275 18 810 147 631 Cuts at: 3418 4307 5936 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 810 631 275 147 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3418 1537 Size: 275 1606 4090 Fragments arranged by size: 4090 1606 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2913 3901 1353 Size: 1560 988 3423 Fragments arranged by size: 3423 1560 988 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3155 4558 4932 5078 387 Size: 1064 138 1566 1403 374 146 1280 Fragments arranged by size: 1566 1403 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2388 2978 3110 3231 4034 4631 5418 Size: 393 756 590 132 121 803 597 787 Cuts at: 5418 5686 1239 Size: 268 1524 Fragments arranged by size: 1524 803 787 756 597 590 393 268 132 121 EcoNI CCTnn'n_nnAGG Cuts at: 2909 2909 Size: 5971 EcoO109I rG'GnC_Cy Cuts at: 2020 2066 2254 2765 2020 Size: 46 188 511 5226 Fragments arranged by size: 5226 511 188 46 EcoRI G'AATT_C Cuts at: 2967 3280 3658 2967 Size: 313 378 5280 Fragments arranged by size: 5280 378 313 EcoRV GAT'ATC Cuts at: 2614 2614 Size: 5971 FspI TGC'GCA Cuts at: 4477 4477 Size: 5971 HaeII r_GCGC'y Cuts at: 1678 2222 2432 3232 5118 1678 Size: 544 210 800 1886 2531 Fragments arranged by size: 2531 1886 800 544 210 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2068 2100 2323 2355 2508 Size: 32 135 32 496 32 223 32 153 Cuts at: 2508 2540 4612 4644 4686 4718 1373 Size: 32 2072 32 42 32 2626 Fragments arranged by size: 2626 2072 496 223 153 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3315 3561 669 Size: 988 281 1377 246 3079 Fragments arranged by size: 3079 1377 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5971 HpaI GTT'AAC Cuts at: 3561 3561 Size: 5971 KasI G'GCGC_C Cuts at: 2428 3228 2428 Size: 800 5171 KpnI G_GTAC'C Cuts at: 3296 3296 Size: 5971 MfeI C'AATT_G Cuts at: 3570 3570 Size: 5971 MluI A'CGCG_T Cuts at: 5847 5847 Size: 5971 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5085 5269 1305 Size: 3780 184 2007 Fragments arranged by size: 3780 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4149 Fragments arranged by size: 4149 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3258 3929 4288 4447 518 Size: 648 2092 671 359 159 2042 Fragments arranged by size: 2092 2042 671 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2609 2879 3387 4064 5212 5457 1606 Size: 1003 270 508 677 1148 245 2120 Fragments arranged by size: 2120 1148 1003 677 508 270 245 NaeI GCC'GGC Cuts at: 2364 2364 Size: 5971 NarI GG'CG_CC Cuts at: 2429 3229 2429 Size: 800 5171 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4480 Fragments arranged by size: 4480 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5971 NgoMIV G'CCGG_C Cuts at: 2362 2362 Size: 5971 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5971 NotI GC'GGCC_GC Cuts at: 3418 3418 Size: 5971 NspI r_CATG'y Cuts at: 1132 1229 3329 4874 1132 Size: 97 2100 1545 2229 Fragments arranged by size: 2229 2100 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5971 PciI A'CATG_T Cuts at: 4870 4870 Size: 5971 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5971 PmeI GTTT'AAAC Cuts at: 3275 3275 Size: 5971 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4040 4072 5553 5585 4040 Size: 32 1481 32 4426 Fragments arranged by size: 4426 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2020 2066 2020 Size: 46 5925 PshAI GACnn'nnGTC Cuts at: 1613 2879 1613 Size: 1266 4705 PsiI TTA'TAA Cuts at: 3541 3541 Size: 5971 PspOMI G'GGCC_C Cuts at: 2764 2764 Size: 5971 PstI C_TGCA'G Cuts at: 830 3260 3323 830 Size: 2430 63 3478 Fragments arranged by size: 3478 2430 63 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5971 SalI G'TCGA_C Cuts at: 1936 3313 1936 Size: 1377 4594 SapI GCTCTTCn'nnn_ Cuts at: 2913 2913 Size: 5971 SbfI CC_TGCA'GG Cuts at: 3323 3323 Size: 5971 ScaI AGT'ACT Cuts at: 4219 5931 4219 Size: 1712 4259 SfcI C'TryA_G Cuts at: 826 1045 2869 3256 3319 4454 5135 5326 826 Size: 219 1824 387 63 1135 681 191 1471 Fragments arranged by size: 1824 1471 1135 681 387 219 191 63 SfoI GGC'GCC Cuts at: 2430 3230 2430 Size: 800 5171 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5971 SmaI CCC'GGG Cuts at: 1872 3298 1872 Size: 1426 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2340 2906 4075 4976 5238 820 Size: 197 926 397 566 1169 901 262 1553 Fragments arranged by size: 1553 1169 926 901 566 397 262 197 SnaBI TAC'GTA Cuts at: 493 3762 493 Size: 3269 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5971 SphI G_CATG'C Cuts at: 1132 3329 1132 Size: 2197 3774 SspI AAT'ATT Cuts at: 5 52 3895 5 Size: 47 3843 2081 Fragments arranged by size: 3843 2081 47 StuI AGG'CCT Cuts at: 1517 2711 1517 Size: 1194 4777 StyI C'CwwG_G Cuts at: 513 1065 2004 2445 2481 2739 3118 3410 513 Size: 552 939 441 36 258 379 292 3074 Fragments arranged by size: 3074 939 552 441 379 292 258 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2584 3998 4015 4168 4353 4798 325 Size: 1255 1004 1414 17 153 185 445 1498 Fragments arranged by size: 1498 1414 1255 1004 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4217 5929 96 Size: 274 80 33 51 1443 2240 1712 138 Fragments arranged by size: 2240 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2482 2514 5931 5963 2482 Size: 32 3417 32 2490 Fragments arranged by size: 3417 2490 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5971 XbaI T'CTAG_A Cuts at: 3307 3307 Size: 5971 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5971 XmaI C'CCGG_G Cuts at: 1870 3296 1870 Size: 1426 4545 XmnI GAAnn'nnTTC Cuts at: 2931 4100 2931 Size: 1169 4802 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3779 276 Size: 53 83 186 936 2245 2468 Fragments arranged by size: 2468 2245 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI