(Linear) (Six-base) MAPSORT of: KIBB8296.seq Check: 4037 from: 1 to: 1266 KIBB8296.orf With 182 enzymes: SgfI * December 27, 2011 16:30 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 383 973 1266 Size: 383 590 293 Fragments arranged by size: 590 383 293 AleI CACnn'nnGTG Cuts at: 0 1253 1266 Size: 1253 13 ApaI G_GGCC'C Cuts at: 0 763 1266 Size: 763 503 ApoI r'AATT_y Cuts at: 0 962 1266 Size: 962 304 AvaI C'yCGr_G Cuts at: 0 36 1214 1266 Size: 36 1178 52 Fragments arranged by size: 1178 52 36 BanI G'GyrC_C Cuts at: 0 183 423 1223 1266 Size: 183 240 800 43 Fragments arranged by size: 800 240 183 43 BanII G_rGCy'C Cuts at: 0 391 763 854 1121 1266 Size: 391 372 91 267 145 Fragments arranged by size: 391 372 267 145 91 BbeI G_GCGC'C Cuts at: 0 427 1227 1266 Size: 427 800 39 Fragments arranged by size: 800 427 39 BbsI GAAGACnn'nnnn_ Cuts at: 0 197 581 1266 Size: 197 384 685 Fragments arranged by size: 685 384 197 BbvCI CC'TCA_GC Cuts at: 0 685 1266 Size: 685 581 BclI T'GATC_A Cuts at: 0 1011 1266 Size: 1011 255 BlpI GC'TnA_GC Cuts at: 0 53 1266 Size: 53 1213 Bme1580I G_kGCm'C Cuts at: 0 763 977 1266 Size: 763 214 289 Fragments arranged by size: 763 289 214 BmgBI CAC'GTC Cuts at: 0 594 1266 Size: 594 672 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1105 1226 1266 Size: 1105 121 40 Fragments arranged by size: 1105 121 40 Bpu10I CC'TnA_GC Cuts at: 0 542 685 729 1266 Size: 542 143 44 537 Fragments arranged by size: 542 537 143 44 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 320 886 1266 Size: 320 566 380 Fragments arranged by size: 566 380 320 BsaI GGTCTCn'nnnn_ Cuts at: 0 319 1266 Size: 319 947 BsaHI Gr'CG_yC Cuts at: 0 424 1224 1262 1266 Size: 424 800 38 4 Fragments arranged by size: 800 424 38 4 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 364 787 1266 Size: 364 423 479 Fragments arranged by size: 479 423 364 BseYI C'CCAG_C Cuts at: 0 555 600 1266 Size: 555 45 666 Fragments arranged by size: 666 555 45 BsiHKAI G_wGCw'C Cuts at: 0 461 1142 1266 Size: 461 681 124 Fragments arranged by size: 681 461 124 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1091 1266 Size: 1091 175 Bsp1286I G_dGCh'C Cuts at: 0 391 461 763 854 977 1121 1142 1266 Size: 391 70 302 91 123 144 21 124 Fragments arranged by size: 391 302 144 124 123 91 70 21 BsrDI GCAATG_nn' Cuts at: 0 1175 1266 Size: 1175 91 BsrFI r'CCGG_y Cuts at: 0 357 1266 Size: 357 909 BssHII G'CGCG_C Cuts at: 0 30 1266 Size: 30 1236 BssSI C'ACGA_G Cuts at: 0 691 1266 Size: 691 575 BstAPI GCAn_nnn'nTGC Cuts at: 0 981 1266 Size: 981 285 BstYI r'GATC_y Cuts at: 0 1109 1266 Size: 1109 157 Bsu36I CC'TnA_GG Cuts at: 0 764 1266 Size: 764 502 BtgI C'CryG_G Cuts at: 0 47 1266 Size: 47 1219 BtsI GCAGTG_nn' Cuts at: 0 1248 1260 1266 Size: 1248 12 6 Fragments arranged by size: 1248 12 6 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 429 464 1266 Size: 429 35 802 Fragments arranged by size: 802 429 35 DrdI GACnn_nn'nnGTC Cuts at: 0 835 1266 Size: 835 431 EaeI y'GGCC_r Cuts at: 0 635 782 1266 Size: 635 147 484 Fragments arranged by size: 635 484 147 EarI CTCTTCn'nnn_ Cuts at: 0 908 1266 Size: 908 358 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1150 1266 Size: 1150 116 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 383 973 1105 1226 1266 Size: 383 590 132 121 40 Fragments arranged by size: 590 383 132 121 40 EcoNI CCTnn'n_nnAGG Cuts at: 0 904 1266 Size: 904 362 EcoO109I rG'GnC_Cy Cuts at: 0 15 61 249 760 1266 Size: 15 46 188 511 506 Fragments arranged by size: 511 506 188 46 15 EcoRI G'AATT_C Cuts at: 0 962 1266 Size: 962 304 EcoRV GAT'ATC Cuts at: 0 609 1266 Size: 609 657 HaeII r_GCGC'y Cuts at: 0 217 427 1227 1266 Size: 217 210 800 39 Fragments arranged by size: 800 217 210 39 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 63 95 318 350 503 535 1266 Size: 63 32 223 32 153 32 731 Fragments arranged by size: 731 223 153 63 32 32 32 KasI G'GCGC_C Cuts at: 0 423 1223 1266 Size: 423 800 43 Fragments arranged by size: 800 423 43 MslI CAynn'nnrTG Cuts at: 0 1253 1266 Size: 1253 13 MspA1I CmG'CkG Cuts at: 0 604 874 1266 Size: 604 270 392 Fragments arranged by size: 604 392 270 NaeI GCC'GGC Cuts at: 0 359 1266 Size: 359 907 NarI GG'CG_CC Cuts at: 0 424 1224 1266 Size: 424 800 42 Fragments arranged by size: 800 424 42 NgoMIV G'CCGG_C Cuts at: 0 357 1266 Size: 357 909 PpuMI rG'GwC_Cy Cuts at: 0 15 61 1266 Size: 15 46 1205 Fragments arranged by size: 1205 46 15 PshAI GACnn'nnGTC Cuts at: 0 874 1266 Size: 874 392 PspOMI G'GGCC_C Cuts at: 0 759 1266 Size: 759 507 PstI C_TGCA'G Cuts at: 0 1255 1266 Size: 1255 11 SapI GCTCTTCn'nnn_ Cuts at: 0 908 1266 Size: 908 358 SfcI C'TryA_G Cuts at: 0 864 1251 1266 Size: 864 387 15 Fragments arranged by size: 864 387 15 SfoI GGC'GCC Cuts at: 0 425 1225 1266 Size: 425 800 41 Fragments arranged by size: 800 425 41 SmlI C'TyrA_G Cuts at: 0 335 901 1266 Size: 335 566 365 Fragments arranged by size: 566 365 335 StuI AGG'CCT Cuts at: 0 706 1266 Size: 706 560 StyI C'CwwG_G Cuts at: 0 440 476 734 1113 1266 Size: 440 36 258 379 153 Fragments arranged by size: 440 379 258 153 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 579 1266 Size: 579 687 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 477 509 1266 Size: 477 32 757 Fragments arranged by size: 757 477 32 XmnI GAAnn'nnTTC Cuts at: 0 926 1266 Size: 926 340 Enzymes that do cut: AcuI AleI ApaI ApoI AvaI BanI BanII BbeI BbsI BbvCI BclI BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI BsaHI BseRI BseYI BsiHKAI BsmBI Bsp1286I BsrDI BsrFI BssHII BssSI BstAPI BstYI Bsu36I BtgI BtsI CspCI DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I KasI MslI MspA1I NaeI NarI NgoMIV PpuMI PshAI PspOMI PstI SapI SfcI SfoI SmlI StuI StyI TaqII TstI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI BfrBI BglI BglII BmrI BmtI BplI BsaAI BsaBI BsaWI BsaXI BsgI BsiEI BsiWI BsmI BspEI BspHI BspMI BsrBI BsrGI BstBI BstEII BstXI BstZ17I BtgZI ClaI DraI DraIII EagI EcoICRI FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI MscI NcoI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI XcmI XhoI XmaI ZraI