################################## # NO CUT ENZYMES ( 169 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HhaI Hin1I HinP1I HindIII HpaI HpaII KasI KpnI KspI MfeI MluI MluNI MroI MscI MslI MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI PstI PvuI RcaI RsaI RsrII SacI SacII SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI Van91I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AccI 3 GTCGAC GT^[AC][GT]AC AclI 740 AACGTT AA^CGTT AcsI 12 GAATTT [GA]^AATT[CT] ApoI 12 GAATTT [GA]^AATT[CT] AspI 599 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC BanII 792 GGGCTC G[GA]GC[CT]^C BbvI 221 GCAGC GCAGC(8/12) BpmI 777 CTCCAG CTGGAG(16/14) BsaJI 180 CCGAGG C^C[ACGT][ACGT]GG BsiYI 773 CCAGCTCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 773 CCAGCTCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BspDI 95 ATCGAT AT^CGAT BssSI 752 CTCGTG CACGAG(-5/-1) BstEII 917 GGTCACC G^GT[ACGT]ACC BstPI 917 GGTCACC G^GT[ACGT]ACC BstXI 930 CCAGGTGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ClaI 95 ATCGAT AT^CGAT EcoO65I 917 GGTCACC G^GT[ACGT]ACC Fnu4HI 221 GCAGC GC^[ACGT]GC HgaI 529 GACGC GACGC(5/10) HincII 3 GTCGAC GT[CT]^[GA]AC HindII 3 GTCGAC GT[CT]^[GA]AC ItaI 221 GCAGC GC^[ACGT]GC MspA1I 87 CAGCTG C[AC]G^C[GT]G PflFI 599 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 20 GAGTC GAGTC(4/5) Psp1406I 740 AACGTT AA^CGTT PvuII 87 CAGCTG CAG^CTG SalI 3 GTCGAC G^TCGAC SapI 794 GCTCTTC GCTCTTC(1/4) SmlI 660 CTCAAG C^T[CT][GA]AG StuI 183 AGGCCT AGG^CCT TseI 221 GCAGC G^C[AT]GC Tth111I 599 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 868 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AlwI 770 GATCC GGATC(4/5) 619 GGATC Asp700I 695 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 860 GAAGTCGTTC BmyI 144 GGGCAC G[AGT]GC[ACT]^C 792 GGGCTC BsaBI 481 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 635 GATCTTCATC Bsp1286I 144 GGGCAC G[AGT]GC[ACT]^C 792 GGGCTC BsrDI 207 GCAATG GCAATG(2/0) 224 GCAATG DdeI 119 CTGAG C^T[ACGT]AG 842 CTGAG DraII 682 AGGTCCC [GA]G^G[ACGT]CC[CT] 781 AGGTCCC EarI 795 CTCTTC CTCTTC(1/4) 469 GAAGAG EcoO109I 682 AGGTCCC [GA]G^G[ACGT]CC[CT] 781 AGGTCCC Ksp632I 795 CTCTTC CTCTTC(1/4) 469 GAAGAG MaeII 384 ACGT A^CGT 741 ACGT MaeIII 26 GTGAC ^GT[ACGT]AC 918 GTCAC MamI 481 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 635 GATCTTCATC MseI 317 TTAA T^TAA 433 TTAA NlaIV 683 GGTCCC GG[ACGT]^[ACGT]CC 782 GGTCCC PpuMI 682 AGGTCCC [GA]G^G[AT]CC[CT] 781 AGGTCCC SfaNI 556 GATGC GCATC(5/9) 424 GCATC TfiI 355 GAATC G^A[AT]TC 454 GAATC Tru9I 317 TTAA T^TAA 433 TTAA Tsp45I 26 GTGAC ^GT[GC]AC 918 GTCAC XmnI 695 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 860 GAAGTCGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AluI AvaII BfaI BglII BsmFI BsrI BssKI BstF5I BstNI BstYI Cfr13I DpnII EcoRII FokI HaeIII HinfI HphI MaeI MboI MboII MflI MnlI MvaI NdeII Sau3AI Sau96I ScrFI TaqI Tsp509I TspRI TthHB8I XhoII