################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII Acc65I AccII AclI AcsI AflII AflIII AgeI AhdI AlwI Aor51HI ApoI AscI AseI AsnI Asp718I AspEI AspI AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BclI BfrI BglI BlnI BsaAI BsaBI BsaI BsiWI BsmAI BsmBI BsmI Bsp1407I BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Cfr10I CpoI DraI DraIII DrdI Eam1105I Eco47III EcoNI EcoO65I EcoRI FbaI FseI HindIII HpaI KasI KpnI KspI MaeII MaeIII MamI MfeI MluI MluNI MscI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tsp45I Tth111I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccI 1099 GTCTAC GT^[AC][GT]AC AcyI 3 GGCGTC G[GA]^CG[CT]C Alw44I 637 GTGCAC G^TGCAC AlwNI 550 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 717 CCTTAGG CC^T[ACGT]AGG ApaI 1026 GGGCCC GGGCC^C ApaLI 637 GTGCAC G^TGCAC AvaI 674 CCCGGG C^[CT]CG[GA]G AviII 608 TGCGCA TGC^GCA BciVI 460 GTATCC GTATCC(6/5) BglII 1196 AGATCT A^GATCT BpmI 787 CTGGAG CTGGAG(16/14) BsaHI 3 GGCGTC G[GA]^CG[CT]C BsoBI 674 CCCGGG C^[CT]CG[GA]G BspDI 338 ATCGAT AT^CGAT BsrDI 474 CATTGC GCAATG(2/0) BstYI 1196 AGATCT [GA]^GATC[CT] Bsu36I 717 CCTTAGG CC^T[ACGT]AGG ClaI 338 ATCGAT AT^CGAT DraII 48 AGGCCCC [GA]G^G[ACGT]CC[CT] EaeI 128 CGGCCG [CT]^GGCC[GA] EagI 128 CGGCCG C^GGCCG EclXI 128 CGGCCG C^GGCCG Eco52I 128 CGGCCG C^GGCCG Eco81I 717 CCTTAGG CC^T[ACGT]AGG EcoO109I 48 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoRV 1120 GATATC GAT^ATC EcoT14I 528 CCATGG C^C[AT][AT]GG EcoT22I 484 ATGCAT ATGCA^T FspI 608 TGCGCA TGC^GCA HaeII 897 AGCGCC [GA]GCGC^[CT] HgaI 4 GCGTC GACGC(5/10) Hin1I 3 GGCGTC G[GA]^CG[CT]C HincII 250 GTCAAC GT[CT]^[GA]AC HindII 250 GTCAAC GT[CT]^[GA]AC MflI 1196 AGATCT [GA]^GATC[CT] MslI 597 CATGCTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 528 CCATGG C^CATGG NsiI 484 ATGCAT ATGCA^T PflMI 549 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 441 GACTCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 936 CTGCAG CTGCA^G PvuI 435 CGATCG CGAT^CG PvuII 823 CAGCTG CAG^CTG SacI 181 GAGCTC GAGCT^C SmaI 674 CCCGGG CCC^GGG SmlI 655 CTCAAG C^T[CT][GA]AG SspI 780 AATATT AAT^ATT StyI 528 CCATGG C^C[AT][AT]GG TfiI 1232 GATTC G^A[AT]TC Tsp509I 707 AATT ^AATT Van91I 549 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 1196 AGATCT [GA]^GATC[CT] XmaCI 674 CCCGGG C^CCGGG XmaI 674 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AccIII 1067 TCCGGA T^CCGGA 1212 TCCGGA AfaI 513 GTAC GT^AC 564 GTAC Asp700I 90 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 114 GAAGGTGTTC AspHI 181 GAGCTC G[AT]GC[AT]^C 637 GTGCAC AvaII 15 GGTCC G^G[AT]CC 949 GGACC BanI 192 GGTGCC G^G[CT][GA]CC 976 GGCACC BlpI 183 GCTCAGC GC^T[ACGT]AGC 1225 GCTAAGC Bpu1102I 183 GCTCAGC GC^T[ACGT]AGC 1225 GCTAAGC BsaWI 1067 TCCGGA [AT]^CCGG[AT] 1212 TCCGGA BseAI 1067 TCCGGA T^CCGGA 1212 TCCGGA BsgI 100 GTGCAG GTGCAG(16/14) 820 GTGCAG BsiEI 128 CGGCCG CG[GA][CT]^CG 435 CGATCG BsiHKAI 181 GAGCTC G[AT]GC[AT]^C 637 GTGCAC BspEI 1067 TCCGGA T^CCGGA 1212 TCCGGA BstAPI 93 GCATTTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1153 GCAGCTCATGC CelII 183 GCTCAGC GC^T[ACGT]AGC 1225 GCTAAGC CfoI 609 GCGC GCG^C 898 GCGC DsaI 26 CCACGG C^C[GA][CT]GG 528 CCATGG EarI 766 GAAGAG CTCTTC(1/4) 798 GAAGAG FauI 135 CCCGC CCCGC(4/6) 906 GCGGG HhaI 609 GCGC GCG^C 898 GCGC HinP1I 609 GCGC G^CGC 898 GCGC Ksp632I 766 GAAGAG CTCTTC(1/4) 798 GAAGAG MroI 1067 TCCGGA T^CCGGA 1212 TCCGGA MseI 227 TTAA T^TAA 701 TTAA MspA1I 136 CCGCTG C[AC]G^C[GT]G 823 CAGCTG NspI 482 ACATGC [GA]CATG^[CT] 596 ACATGC RsaI 513 GTAC GT^AC 564 GTAC SfcI 840 CTACAG C^T[GA][CT]AG 936 CTGCAG TaqI 172 TCGA T^CGA 339 TCGA Tru9I 227 TTAA T^TAA 701 TTAA TthHB8I 172 TCGA T^CGA 339 TCGA XmnI 90 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 114 GAAGGTGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AciI AluI BanII BbsI BbvI BcnI BfaI BmyI BpuAI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MboI MboII MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI TseI TspRI