Restriction map for
pFN21AE3653
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccIII AclI AfaI
AflII AflIII AgeI Aor51HI ApaI
AscI AseI AsnI Asp718I AspI
AviII AvrII BanI BbeI BbrPI
BbvCI BcgI BciVI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BsgI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I CelII ClaI
CpoI DraI DrdI EarI Eco47III
EcoNI EcoO65I EcoRV EcoT14I EcoT22I
FseI FspI HaeII HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeI MamI MfeI MluI
MroI MseI MslI MspA1I MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI ScaI SexAI
SfiI SfoI SfuI SmaI SmlI
SnaBI SpeI SphI SspBI SspI
StuI StyI SwaI TaqI Tru9I
Tth111I TthHB8I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 820 GTCTAC GT^[AC][GT]AC
AccII 246 CGCG CG^CG
AcsI 485 GAATTC [GA]^AATT[CT]
AcyI 169 GACGCC G[GA]^CG[CT]C
Alw44I 592 GTGCAC G^TGCAC
AocI 601 CCTAAGG CC^T[ACGT]AGG
ApaLI 592 GTGCAC G^TGCAC
ApoI 485 GAATTC [GA]^AATT[CT]
Asp700I 333 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 663 CCCGAG C^[CT]CG[GA]G
BalI 444 TGGCCA TGG^CCA
BanII 582 GAGCCC G[GA]GC[CT]^C
BbsI 166 GAAGAC GAAGAC(2/6)
BclI 44 TGATCA T^GATCA
BpmI 610 CTCCAG CTGGAG(16/14)
BpuAI 166 GAAGAC GAAGAC(2/6)
BsaHI 169 GACGCC G[GA]^CG[CT]C
BseRI 522 CTCCTC GAGGAG(10/8)
BsoBI 663 CCCGAG C^[CT]CG[GA]G
BspMI 706 GCAGGT ACCTGC(4/8)
BsrDI 752 GCAATG GCAATG(2/0)
BsrFI 694 GCCGGT [GA]^CCGG[CT]
BssSI 772 CTCGTG CACGAG(-5/-1)
BstUI 246 CGCG CG^CG
Bsu36I 601 CCTAAGG CC^T[ACGT]AGG
Cfr10I 694 GCCGGT [GA]^CCGG[CT]
DraIII 843 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 235 CCGTGG C^C[GA][CT]GG
EagI 314 CGGCCG C^GGCCG
EclXI 314 CGGCCG C^GGCCG
Eco52I 314 CGGCCG C^GGCCG
Eco81I 601 CCTAAGG CC^T[ACGT]AGG
EcoRI 485 GAATTC G^AATTC
FauI 278 GCGGG CCCGC(4/6)
FbaI 44 TGATCA T^GATCA
HgaI 169 GACGC GACGC(5/10)
Hin1I 169 GACGCC G[GA]^CG[CT]C
MaeII 518 ACGT A^CGT
MluNI 444 TGGCCA TGG^CCA
MscI 444 TGGCCA TGG^CCA
MvnI 246 CGCG CG^CG
NspI 75 GCATGT [GA]CATG^[CT]
PflMI 141 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 770 GACTCGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 762 CCTGCAGG CCTGCA^GG
SgrAI 693 CGCCGGTG C[GA]^CCGG[CT]G
Sse8387I 762 CCTGCAGG CCTGCA^GG
Tsp509I 486 AATT ^AATT
Van91I 141 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 723 CCAGGGTGATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 333 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AhdI 169 GACGCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
812 GACCGGGTGTC
AluI 153 AGCT AG^CT
269 AGCT
AlwNI 142 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
301 CAGTGGCTG
AspEI 169 GACGCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
812 GACCGGGTGTC
AvaII 144 GGACC G^G[AT]CC
606 GGTCC
BamHI 208 GGATCC G^GATCC
851 GGATCC
BsiEI 175 CGGTCG CG[GA][CT]^CG
314 CGGCCG
BsmBI 349 CGTCTC CGTCTC(1/5)
519 CGTCTC
BstYI 208 GGATCC [GA]^GATC[CT]
851 GGATCC
Cac8I 240 GCAGGC GC[ACGT]^[ACGT]GC
629 GCGTGC
CfoI 247 GCGC GCG^C
692 GCGC
Eam1105I 169 GACGCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
812 GACCGGGTGTC
HhaI 247 GCGC GCG^C
692 GCGC
HinP1I 247 GCGC G^CGC
692 GCGC
MflI 208 GGATCC [GA]^GATC[CT]
851 GGATCC
PpuMI 143 AGGACCT [GA]G^G[AT]CC[CT]
605 AGGTCCT
PstI 324 CTGCAG CTGCA^G
763 CTGCAG
TfiI 382 GAATC G^A[AT]TC
730 GATTC
XhoII 208 GGATCC [GA]^GATC[CT]
851 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AlwI AspHI BbvI BcnI
BmyI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
DraII EaeI EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeIII MboI MboII
MnlI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI SfaNI SfcI TseI
Tsp45I TspRI