Restriction map for
pFN21AE3653
(Linear) (Six-base) MAPSORT of: KIEE3653.seq Check: 7310 from: 1 to: 879
KIEE3653.orf
With 182 enzymes: SgfI *
December 27, 2011 16:37 ..
AccI GT'mk_AC
Cuts at: 0 821 879
Size: 821 58
AhdI GACnn_n'nnGTC
Cuts at: 0 174 817 879
Size: 174 643 62
Fragments arranged by size:
643 174 62
AlwNI CAG_nnn'CTG
Cuts at: 0 147 306 879
Size: 147 159 573
Fragments arranged by size:
573 159 147
ApaLI G'TGCA_C
Cuts at: 0 592 879
Size: 592 287
ApoI r'AATT_y
Cuts at: 0 485 879
Size: 485 394
AvaI C'yCGr_G
Cuts at: 0 663 879
Size: 663 216
BamHI G'GATC_C
Cuts at: 0 208 851 879
Size: 208 643 28
Fragments arranged by size:
643 208 28
BanII G_rGCy'C
Cuts at: 0 586 879
Size: 586 293
BbsI GAAGACnn'nnnn_
Cuts at: 0 173 879
Size: 173 706
BclI T'GATC_A
Cuts at: 0 44 879
Size: 44 835
Bme1580I G_kGCm'C
Cuts at: 0 128 596 801 879
Size: 128 468 205 78
Fragments arranged by size:
468 205 128 78
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 367 879
Size: 367 512
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 879
Size: 595 284
Bpu10I CC'TnA_GC
Cuts at: 0 256 879
Size: 256 623
BsaHI Gr'CG_yC
Cuts at: 0 170 879
Size: 170 709
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 513 879
Size: 513 366
BseYI C'CCAG_C
Cuts at: 0 154 230 541 566 879
Size: 154 76 311 25 313
Fragments arranged by size:
313 311 154 76 25
BsiEI CG_ry'CG
Cuts at: 0 178 317 879
Size: 178 139 562
Fragments arranged by size:
562 178 139
BsiHKAI G_wGCw'C
Cuts at: 0 35 42 596 879
Size: 35 7 554 283
Fragments arranged by size:
554 283 35 7
BsmBI CGTCTCn'nnnn_
Cuts at: 0 355 525 879
Size: 355 170 354
Fragments arranged by size:
355 354 170
Bsp1286I G_dGCh'C
Cuts at: 0 35 42 128 586 596 801 879
Size: 35 7 86 458 10 205 78
Fragments arranged by size:
458 205 86 78 35 10 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 697 879
Size: 697 182
BsrDI GCAATG_nn'
Cuts at: 0 759 879
Size: 759 120
BsrFI r'CCGG_y
Cuts at: 0 694 879
Size: 694 185
BssSI C'ACGA_G
Cuts at: 0 772 879
Size: 772 107
BstYI r'GATC_y
Cuts at: 0 208 851 879
Size: 208 643 28
Fragments arranged by size:
643 208 28
Bsu36I CC'TnA_GG
Cuts at: 0 602 879
Size: 602 277
BtgI C'CryG_G
Cuts at: 0 235 879
Size: 235 644
BtsI GCAGTG_nn'
Cuts at: 0 321 879
Size: 321 558
DraIII CAC_nnn'GTG
Cuts at: 0 848 879
Size: 848 31
EaeI y'GGCC_r
Cuts at: 0 314 354 444 879
Size: 314 40 90 435
Fragments arranged by size:
435 314 90 40
EagI C'GGCC_G
Cuts at: 0 314 879
Size: 314 565
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 879
Size: 595 284
EcoO109I rG'GnC_Cy
Cuts at: 0 144 606 739 879
Size: 144 462 133 140
Fragments arranged by size:
462 144 140 133
EcoRI G'AATT_C
Cuts at: 0 485 879
Size: 485 394
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 97 129 160 192 803 835 879
Size: 97 32 31 32 611 32 44
Fragments arranged by size:
611 97 44 32 32 32 31
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 236 879
Size: 158 78 643
Fragments arranged by size:
643 158 78
MscI TGG'CCA
Cuts at: 0 446 879
Size: 446 433
NspI r_CATG'y
Cuts at: 0 79 879
Size: 79 800
PflMI CCAn_nnn'nTGG
Cuts at: 0 147 879
Size: 147 732
PfoI T'CCnGG_A
Cuts at: 0 854 879
Size: 854 25
PpuMI rG'GwC_Cy
Cuts at: 0 144 606 879
Size: 144 462 273
Fragments arranged by size:
462 273 144
PshAI GACnn'nnGTC
Cuts at: 0 774 879
Size: 774 105
PstI C_TGCA'G
Cuts at: 0 328 767 879
Size: 328 439 112
Fragments arranged by size:
439 328 112
SbfI CC_TGCA'GG
Cuts at: 0 767 879
Size: 767 112
SfcI C'TryA_G
Cuts at: 0 5 324 763 879
Size: 5 319 439 116
Fragments arranged by size:
439 319 116 5
SgrAI Cr'CCGG_yG
Cuts at: 0 694 879
Size: 694 185
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 18 244 879
Size: 18 226 635
Fragments arranged by size:
635 226 18
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 730 879
Size: 730 149
XmnI GAAnn'nnTTC
Cuts at: 0 337 879
Size: 337 542
Enzymes that do cut:
AccI AhdI AlwNI ApaLI ApoI AvaI BamHI BanII
BbsI BclI Bme1580I BplI BpmI Bpu10I BsaHI BseRI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrFI
BssSI BstYI Bsu36I BtgI BtsI DraIII EaeI EagI
Eco57MI EcoO109I EcoRI Hin4I MmeI MscI NspI PflMI
PfoI PpuMI PshAI PstI SbfI SfcI SgrAI TaqII
XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AlfI AloI ApaI AscI AseI AsiSI
AvrII BaeI BanI BbeI BbvCI BcgI BciVI BfrBI
BglI BglII BlpI BmgBI BmrI BmtI BpuEI BsaI
BsaAI BsaBI BsaWI BsaXI BsgI BsiWI BsmI BspEI
BspHI BsrBI BsrGI BssHII BstAPI BstBI BstEII BstXI
BstZ17I BtgZI ClaI CspCI DraI DrdI EarI EciI
EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PmeI PmlI PpiI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI ScaI SexAI SfiI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TatI TstI Tth111I XbaI XhoI
XmaI ZraI