Restriction map for
pFN21AB7275
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# NO CUT ENZYMES ( 120 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI AspEI AspI AviII BamHI
BbeI BbrPI BbvCI BglI BglII
BlpI Bpu1102I BsaAI BsaHI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstYI BstZ17I Bsu36I CelII
CfoI ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII HpaI KasI KspI
MfeI MflI MluI MroI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SwaI Tth111I XbaI XhoI XhoII
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 2142 GGTACC G^GTACC
AflII 52 CTTAAG C^TTAAG
AlwNI 1396 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 572 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2142 GGTACC G^GTACC
AspHI 1220 GAGCTC G[AT]GC[AT]^C
AvaI 2510 CCCGGG C^[CT]CG[GA]G
AvrII 1837 CCTAGG C^CTAGG
BalI 2268 TGGCCA TGG^CCA
BanII 1220 GAGCTC G[GA]GC[CT]^C
BfrI 52 CTTAAG C^TTAAG
BlnI 1837 CCTAGG C^CTAGG
BsaBI 688 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 313 GAGACC GGTCTC(1/5)
BsaWI 2379 ACCGGA [AT]^CCGG[AT]
BsiHKAI 1220 GAGCTC G[AT]GC[AT]^C
BsmI 1577 GCATTC GAATGC(1/-1)
BsoBI 2510 CCCGGG C^[CT]CG[GA]G
BspHI 1236 TCATGA T^CATGA
BsrFI 206 GCCGGT [GA]^CCGG[CT]
BstXI 739 CCAGCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 206 GCCGGT [GA]^CCGG[CT]
DraII 1200 AGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 2268 TGGCCA [CT]^GGCC[GA]
EarI 886 GAAGAG CTCTTC(1/4)
EcoO109I 1200 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 179 ATGCAT ATGCA^T
HincII 12 GTTGAC GT[CT]^[GA]AC
HindII 12 GTTGAC GT[CT]^[GA]AC
KpnI 2142 GGTACC GGTAC^C
Ksp632I 886 GAAGAG CTCTTC(1/4)
MamI 688 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2268 TGGCCA TGG^CCA
MscI 2268 TGGCCA TGG^CCA
NsiI 179 ATGCAT ATGCA^T
PshAI 1861 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1236 TCATGA T^CATGA
SacI 1220 GAGCTC GAGCT^C
ScaI 1747 AGTACT AGT^ACT
SmaI 2510 CCCGGG CCC^GGG
SmlI 52 CTTAAG C^T[CT][GA]AG
XcmI 2467 CCAGAAGCTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2510 CCCGGG C^CCGGG
XmaI 2510 CCCGGG C^CCGGG
XmnI 572 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AflIII 565 ACATGT A^C[GA][CT]GT
1190 ACATGT
AlwI 817 GGATC GGATC(4/5)
1389 GGATC
BanI 1632 GGTGCC G^G[CT][GA]CC
2142 GGTACC
BbsI 256 GAAGAC GAAGAC(2/6)
1515 GAAGAC
BcgI 232 CGAGGTGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1197 CGAAGGGCCTGC
BclI 98 TGATCA T^GATCA
1296 TGATCA
BfaI 1838 CTAG C^TAG
2132 CTAG
BmyI 453 GTGCCC G[AGT]GC[ACT]^C
1220 GAGCTC
BpmI 2282 CTCCAG CTGGAG(16/14)
196 CTGGAG
BpuAI 256 GAAGAC GAAGAC(2/6)
1515 GAAGAC
BseRI 1436 GAGGAG GAGGAG(10/8)
1660 GAGGAG
BsgI 1342 CTGCAC GTGCAG(16/14)
2563 GTGCAG
Bsp1286I 453 GTGCCC G[AGT]GC[ACT]^C
1220 GAGCTC
BspLU11I 565 ACATGT A^CATGT
1190 ACATGT
DraI 789 TTTAAA TTT^AAA
1507 TTTAAA
EcoRI 572 GAATTC G^AATTC
2158 GAATTC
FauI 1211 CCCGC CCCGC(4/6)
1995 GCGGG
FbaI 98 TGATCA T^GATCA
1296 TGATCA
MaeI 1838 CTAG C^TAG
2132 CTAG
PflMI 1116 CCATGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1591 CCAAACAATGG
PstI 159 CTGCAG CTGCA^G
1846 CTGCAG
PvuII 156 CAGCTG CAG^CTG
1753 CAGCTG
SfcI 159 CTGCAG C^T[GA][CT]AG
1846 CTGCAG
TaqI 279 TCGA T^CGA
1367 TCGA
TthHB8I 279 TCGA T^CGA
1367 TCGA
Van91I 1116 CCATGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1591 CCAAACAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BbvI BciVI BcnI BsaJI
BsiYI BslI BsmAI BsmFI BspMI
BsrDI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DsaI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SspI StuI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI