(Circular) (Six-base) MAPSORT of: pFN21AB7275.seq Check: 3437 from: 1 to: 7330 pFN21AB7275 7330 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2984 2984 Size: 7330 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5140 278 Size: 53 83 186 936 3604 2468 Fragments arranged by size: 3604 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4673 1937 Size: 2736 4594 Acc65I G'GTAC_C Cuts at: 4147 4651 4147 Size: 504 6826 AclI AA'CG_TT Cuts at: 1563 5457 5830 1563 Size: 3894 373 3063 Fragments arranged by size: 3894 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3092 3546 3653 4482 5393 6777 7045 1632 Size: 1460 454 107 829 911 1384 268 1917 Fragments arranged by size: 1917 1460 1384 911 829 454 268 107 AflII C'TTAA_G Cuts at: 820 1017 2057 820 Size: 197 1040 6093 Fragments arranged by size: 6093 1040 197 AflIII A'CryG_T Cuts at: 1203 2570 3195 6229 7206 1203 Size: 1367 625 3034 977 1327 Fragments arranged by size: 3034 1367 1327 977 625 AgeI A'CCGG_T Cuts at: 6161 6161 Size: 7330 AhdI GACnn_n'nnGTC Cuts at: 6059 6059 Size: 7330 AleI CACnn'nnGTG Cuts at: 1166 2270 3093 1166 Size: 1104 823 5403 Fragments arranged by size: 5403 1104 823 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7330 AlwNI CAG_nnn'CTG Cuts at: 3406 5073 6645 3406 Size: 1667 1572 4091 Fragments arranged by size: 4091 1667 1572 ApaLI G'TGCA_C Cuts at: 5387 6543 5387 Size: 1156 6174 ApoI r'AATT_y Cuts at: 1493 2303 2577 2710 3082 3823 4163 4584 4639 Size: 810 274 133 372 741 340 421 55 Cuts at: 4639 4865 5017 7126 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 810 741 421 372 340 274 226 152 133 55 AseI AT'TA_AT Cuts at: 160 1032 5884 160 Size: 872 4852 1606 Fragments arranged by size: 4852 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7330 AvaI C'yCGr_G Cuts at: 1870 1943 4515 4655 1870 Size: 73 2572 140 4545 Fragments arranged by size: 4545 2572 140 73 AvrII C'CTAG_G Cuts at: 3842 3842 Size: 7330 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4139 4172 4139 Size: 33 7297 BamHI G'GATC_C Cuts at: 4660 5027 4660 Size: 367 6963 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3637 4147 4651 6106 618 Size: 325 217 627 1850 510 504 1455 1842 Fragments arranged by size: 1850 1842 1455 627 510 504 325 217 BanII G_rGCy'C Cuts at: 1391 3229 4649 1391 Size: 1838 1420 4072 Fragments arranged by size: 4072 1838 1420 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2268 3527 928 Size: 971 369 1259 4731 Fragments arranged by size: 4731 1259 971 369 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2226 2260 3191 3225 5521 5555 1903 Size: 34 289 34 931 34 2296 34 3678 Fragments arranged by size: 3678 2296 931 289 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2530 2616 2664 5222 6432 2530 Size: 86 48 2558 1210 3428 Fragments arranged by size: 3428 2558 1210 86 48 BclI T'GATC_A Cuts at: 1548 2103 3301 1548 Size: 555 1198 5577 Fragments arranged by size: 5577 1198 555 BfrBI ATG'CAT Cuts at: 2186 2186 Size: 7330 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5941 136 Size: 107 122 71 631 605 4269 1525 Fragments arranged by size: 4269 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7325 7325 Size: 7330 BlpI GC'TnA_GC Cuts at: 4747 4747 Size: 7330 Bme1580I G_kGCm'C Cuts at: 1790 2462 5391 6547 1790 Size: 672 2929 1156 2573 Fragments arranged by size: 2929 2573 1156 672 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7330 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3501 3946 4551 Size: 416 53 83 399 24 2084 445 605 Cuts at: 4551 5061 6019 442 Size: 510 958 1753 Fragments arranged by size: 2084 1753 958 605 510 445 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7330 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2222 4272 5990 1239 Size: 983 2050 1718 2579 Fragments arranged by size: 2579 2050 1718 983 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5455 6320 6618 5455 Size: 865 298 6167 Fragments arranged by size: 6167 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2312 5993 882 Size: 617 813 3681 2219 Fragments arranged by size: 3681 2219 813 617 BsaAI yAC'GTr Cuts at: 493 1204 5121 493 Size: 711 3917 2702 Fragments arranged by size: 3917 2702 711 BsaBI GATnn'nnATC Cuts at: 2697 7324 2697 Size: 4627 2703 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5137 5519 6154 275 Size: 53 83 186 936 3604 382 635 1451 Fragments arranged by size: 3604 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4384 5763 6161 6435 6582 6993 4384 Size: 1379 398 274 147 411 4721 Fragments arranged by size: 4721 1379 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4221 4251 1178 Size: 30 165 30 2818 30 4257 Fragments arranged by size: 4257 2818 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3456 3680 1186 Size: 2270 224 4836 Fragments arranged by size: 4836 2270 224 BseYI C'CCAG_C Cuts at: 2250 2445 6533 7029 2250 Size: 195 4088 496 2551 Fragments arranged by size: 4088 2551 496 195 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3332 4589 1163 Size: 573 1596 1257 3904 Fragments arranged by size: 3904 1596 1257 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4780 5541 6569 1540 Size: 275 186 2779 761 1028 2301 Fragments arranged by size: 2779 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3229 5391 5476 6547 3229 Size: 2162 85 1071 4012 Fragments arranged by size: 4012 2162 1071 85 BsmI GAATG_Cn' Cuts at: 3582 4841 4934 3582 Size: 1259 93 5978 Fragments arranged by size: 5978 1259 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2462 3229 4649 5391 5476 6547 1391 Size: 399 672 767 1420 742 85 1071 2174 Fragments arranged by size: 2174 1420 1071 767 742 672 399 85 BspEI T'CCGG_A Cuts at: 6993 6993 Size: 7330 BspHI T'CATG_A Cuts at: 3241 5219 6949 3241 Size: 1978 1730 3622 Fragments arranged by size: 3622 1978 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2484 2934 2984 4685 844 Size: 1640 450 50 1701 3489 Fragments arranged by size: 3489 1701 1640 450 50 BsrBI CCG'CTC Cuts at: 1394 1823 5217 1394 Size: 429 3394 3507 Fragments arranged by size: 3507 3394 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2787 3704 4509 5825 5999 65 Size: 1183 1539 917 805 1316 174 1396 Fragments arranged by size: 1539 1396 1316 1183 917 805 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2211 5974 6161 1700 Size: 110 401 3763 187 2869 Fragments arranged by size: 3763 2869 401 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7330 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7330 BssSI C'ACGA_G Cuts at: 5390 6402 5390 Size: 1012 6318 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7330 BstBI TT'CG_AA Cuts at: 6134 6134 Size: 7330 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7330 BstXI CCAn_nnnn'nTGG Cuts at: 2751 2751 Size: 7330 BstYI r'GATC_y Cuts at: 1972 4660 5027 5412 5429 6881 6967 6979 7325 Size: 2688 367 385 17 1452 86 12 346 Cuts at: 7325 1972 Size: 1977 Fragments arranged by size: 2688 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3121 3451 3610 4072 513 Size: 552 939 1117 330 159 462 3771 Fragments arranged by size: 3771 1117 939 552 462 330 159 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3951 487 Size: 683 56 2725 3866 Fragments arranged by size: 3866 2725 683 56 BtsI GCAGTG_nn' Cuts at: 4846 5640 5660 4846 Size: 794 20 6516 Fragments arranged by size: 6516 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7330 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2750 2785 529 Size: 35 2186 35 5074 Fragments arranged by size: 5074 2186 35 35 DraI TTT'AAA Cuts at: 2796 3514 4634 2796 Size: 718 1120 5492 Fragments arranged by size: 5492 1120 718 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6337 809 Size: 507 5021 1802 Fragments arranged by size: 5021 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4273 4777 5666 Size: 54 1425 50 275 18 2443 504 889 Cuts at: 5666 7295 8 Size: 1629 43 Fragments arranged by size: 2443 1629 1425 889 504 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4777 1537 Size: 275 2965 4090 Fragments arranged by size: 4090 2965 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2886 5260 1353 Size: 1533 2374 3423 Fragments arranged by size: 3423 2374 1533 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5917 6291 6437 387 Size: 1064 138 4328 374 146 1280 Fragments arranged by size: 4328 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3227 3227 Size: 7330 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2222 3092 3546 3653 4272 4482 5393 Size: 393 590 870 454 107 619 210 911 Cuts at: 5393 5990 6777 7045 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 911 870 787 619 597 590 454 393 268 210 107 EcoO109I rG'GnC_Cy Cuts at: 3206 3206 Size: 7330 EcoRI G'AATT_C Cuts at: 2577 4163 4639 5017 2577 Size: 1586 476 378 4890 Fragments arranged by size: 4890 1586 476 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2194 2194 Size: 7330 FspI TGC'GCA Cuts at: 5836 5836 Size: 7330 HaeII r_GCGC'y Cuts at: 1678 6477 1678 Size: 4799 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3656 3688 3769 3801 5971 Size: 32 135 32 2084 32 81 32 2170 Cuts at: 5971 6003 6045 6077 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2170 2084 135 81 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2019 4674 4920 669 Size: 988 281 81 2655 246 3079 Fragments arranged by size: 3079 2655 988 281 246 81 HindIII A'AGCT_T Cuts at: 748 748 Size: 7330 HpaI GTT'AAC Cuts at: 4920 4920 Size: 7330 KpnI G_GTAC'C Cuts at: 4151 4655 4151 Size: 504 6826 MfeI C'AATT_G Cuts at: 4929 4929 Size: 7330 MluI A'CGCG_T Cuts at: 7206 7206 Size: 7330 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6444 6628 1305 Size: 5139 184 2007 Fragments arranged by size: 5139 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4275 10 Size: 54 1425 343 2443 3065 Fragments arranged by size: 3065 2443 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2270 2861 2994 3093 4281 5288 5647 Size: 648 1104 591 133 99 1188 1007 359 Cuts at: 5647 5806 518 Size: 159 2042 Fragments arranged by size: 2042 1188 1104 1007 648 591 359 159 133 99 MspA1I CmG'CkG Cuts at: 1606 2163 3079 3760 4746 5423 6571 6816 1606 Size: 557 916 681 986 677 1148 245 2120 Fragments arranged by size: 2120 1148 986 916 681 677 557 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3121 3451 3610 513 Size: 552 939 1117 330 159 4233 Fragments arranged by size: 4233 1117 939 552 330 159 NdeI CA'TA_TG Cuts at: 387 387 Size: 7330 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7330 NotI GC'GGCC_GC Cuts at: 4777 4777 Size: 7330 NsiI A_TGCA'T Cuts at: 2188 2188 Size: 7330 NspI r_CATG'y Cuts at: 1132 1229 2229 2574 2866 2993 3199 4214 4688 Size: 97 1000 345 292 127 206 1015 474 Cuts at: 4688 6233 1132 Size: 1545 2229 Fragments arranged by size: 2229 1545 1015 1000 474 345 292 206 127 97 PasI CC'CwG_GG Cuts at: 1348 4397 1348 Size: 3049 4281 PciI A'CATG_T Cuts at: 2570 3195 6229 2570 Size: 625 3034 3671 Fragments arranged by size: 3671 3034 625 PflMI CCAn_nnn'nTGG Cuts at: 1108 3127 3602 1108 Size: 2019 475 4836 Fragments arranged by size: 4836 2019 475 PmeI GTTT'AAAC Cuts at: 4634 4634 Size: 7330 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5399 5431 6912 6944 5399 Size: 32 1481 32 5785 Fragments arranged by size: 5785 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3870 1613 Size: 2257 5073 PsiI TTA'TAA Cuts at: 3459 4205 4263 4900 3459 Size: 746 58 637 5889 Fragments arranged by size: 5889 746 637 58 PstI C_TGCA'G Cuts at: 830 2168 3855 4682 830 Size: 1338 1687 827 3478 Fragments arranged by size: 3478 1687 1338 827 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7330 PvuII CAG'CTG Cuts at: 2163 3760 2163 Size: 1597 5733 SacI G_AGCT'C Cuts at: 3229 3229 Size: 7330 SalI G'TCGA_C Cuts at: 1936 4672 1936 Size: 2736 4594 SbfI CC_TGCA'GG Cuts at: 4682 4682 Size: 7330 ScaI AGT'ACT Cuts at: 3754 5578 7290 3754 Size: 1824 1712 3794 Fragments arranged by size: 3794 1824 1712 SfcI C'TryA_G Cuts at: 826 1045 2164 3851 4678 5813 6494 6685 826 Size: 219 1119 1687 827 1135 681 191 1471 Fragments arranged by size: 1687 1471 1135 1119 827 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7330 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7330 SmaI CCC'GGG Cuts at: 1872 4517 4657 1872 Size: 2645 140 4545 Fragments arranged by size: 4545 2645 140 SmlI C'TyrA_G Cuts at: 820 1017 1943 2057 5434 6335 6597 820 Size: 197 926 114 3377 901 262 1553 Fragments arranged by size: 3377 1553 926 901 262 197 114 SnaBI TAC'GTA Cuts at: 493 5121 493 Size: 4628 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7330 SphI G_CATG'C Cuts at: 1132 4688 1132 Size: 3556 3774 SspI AAT'ATT Cuts at: 5 52 2522 3547 4254 5254 5 Size: 47 2470 1025 707 1000 2081 Fragments arranged by size: 2470 2081 1025 1000 707 47 StuI AGG'CCT Cuts at: 1517 2124 3069 3841 1517 Size: 607 945 772 5006 Fragments arranged by size: 5006 945 772 607 StyI C'CwwG_G Cuts at: 513 1065 2004 2404 3121 3451 3610 3842 4769 Size: 552 939 400 717 330 159 232 927 Cuts at: 4769 513 Size: 3074 Fragments arranged by size: 3074 939 927 717 552 400 330 232 159 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2250 3806 4085 4389 5357 5374 5527 Size: 1255 670 1556 279 304 968 17 153 Cuts at: 5527 5712 6157 325 Size: 185 445 1498 Fragments arranged by size: 1556 1498 1255 968 670 445 304 279 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3752 5576 7288 Size: 274 80 33 51 1443 1775 1824 1712 Cuts at: 7288 96 Size: 138 Fragments arranged by size: 1824 1775 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2814 2846 3386 3418 4221 4253 4309 4341 7290 Size: 32 540 32 803 32 56 32 2949 Cuts at: 7290 7322 2814 Size: 32 2822 Fragments arranged by size: 2949 2822 803 540 56 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7330 XbaI T'CTAG_A Cuts at: 4666 4666 Size: 7330 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4479 4479 Size: 7330 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7330 XmaI C'CCGG_G Cuts at: 1870 4515 4655 1870 Size: 2645 140 4545 Fragments arranged by size: 4545 2645 140 XmnI GAAnn'nnTTC Cuts at: 2581 5459 2581 Size: 2878 4452 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5138 276 Size: 53 83 186 936 3604 2468 Fragments arranged by size: 3604 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI