Restriction map for
pFN21AB7275
(Linear) (Six-base) MAPSORT of: KIBB7275.seq Check: 4430 from: 1 to: 2625
KIBB7275.orf
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 979 2625
Size: 979 1646
Acc65I G'GTAC_C
Cuts at: 0 2142 2625
Size: 2142 483
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1087 1541 1648 2477 2625
Size: 1087 454 107 829 148
Fragments arranged by size:
1087 829 454 148 107
AflII C'TTAA_G
Cuts at: 0 52 2625
Size: 52 2573
AflIII A'CryG_T
Cuts at: 0 565 1190 2625
Size: 565 625 1435
Fragments arranged by size:
1435 625 565
AleI CACnn'nnGTG
Cuts at: 0 265 1088 2625
Size: 265 823 1537
Fragments arranged by size:
1537 823 265
AlwNI CAG_nnn'CTG
Cuts at: 0 1401 2625
Size: 1401 1224
ApoI r'AATT_y
Cuts at: 0 298 572 705 1077 1818 2158 2579 2625
Size: 298 274 133 372 741 340 421 46
Fragments arranged by size:
741 421 372 340 298 274 133 46
AvaI C'yCGr_G
Cuts at: 0 2510 2625
Size: 2510 115
AvrII C'CTAG_G
Cuts at: 0 1837 2625
Size: 1837 788
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2134 2167 2625
Size: 2134 33 458
Fragments arranged by size:
2134 458 33
BanI G'GyrC_C
Cuts at: 0 1632 2142 2625
Size: 1632 510 483
Fragments arranged by size:
1632 510 483
BanII G_rGCy'C
Cuts at: 0 1224 2625
Size: 1224 1401
BbsI GAAGACnn'nnnn_
Cuts at: 0 263 1522 2625
Size: 263 1259 1103
Fragments arranged by size:
1259 1103 263
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 221 255 1186 1220 2625
Size: 221 34 931 34 1405
Fragments arranged by size:
1405 931 221 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 525 611 659 2625
Size: 525 86 48 1966
Fragments arranged by size:
1966 525 86 48
BclI T'GATC_A
Cuts at: 0 98 1296 2625
Size: 98 1198 1329
Fragments arranged by size:
1329 1198 98
BfrBI ATG'CAT
Cuts at: 0 181 2625
Size: 181 2444
Bme1580I G_kGCm'C
Cuts at: 0 457 2625
Size: 457 2168
BmrI ACTGGGnnnn_n'
Cuts at: 0 1496 1941 2546 2625
Size: 1496 445 605 79
Fragments arranged by size:
1496 605 445 79
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 2267 2625
Size: 217 2050 358
Fragments arranged by size:
2050 358 217
BsaI GGTCTCn'nnnn_
Cuts at: 0 307 2625
Size: 307 2318
BsaBI GATnn'nnATC
Cuts at: 0 692 2625
Size: 692 1933
BsaWI w'CCGG_w
Cuts at: 0 2379 2625
Size: 2379 246
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2216 2246 2625
Size: 2216 30 379
Fragments arranged by size:
2216 379 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1451 1675 2625
Size: 1451 224 950
Fragments arranged by size:
1451 950 224
BseYI C'CCAG_C
Cuts at: 0 245 440 2625
Size: 245 195 2185
Fragments arranged by size:
2185 245 195
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1327 2584 2625
Size: 1327 1257 41
Fragments arranged by size:
1327 1257 41
BsiHKAI G_wGCw'C
Cuts at: 0 1224 2625
Size: 1224 1401
BsmI GAATG_Cn'
Cuts at: 0 1577 2625
Size: 1577 1048
Bsp1286I G_dGCh'C
Cuts at: 0 457 1224 2625
Size: 457 767 1401
Fragments arranged by size:
1401 767 457
BspHI T'CATG_A
Cuts at: 0 1236 2625
Size: 1236 1389
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 479 929 979 2625
Size: 479 450 50 1646
Fragments arranged by size:
1646 479 450 50
BsrDI GCAATG_nn'
Cuts at: 0 782 1699 2504 2625
Size: 782 917 805 121
Fragments arranged by size:
917 805 782 121
BsrFI r'CCGG_y
Cuts at: 0 206 2625
Size: 206 2419
BstXI CCAn_nnnn'nTGG
Cuts at: 0 746 2625
Size: 746 1879
BtgI C'CryG_G
Cuts at: 0 1116 1446 1605 2067 2625
Size: 1116 330 159 462 558
Fragments arranged by size:
1116 558 462 330 159
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1946 2625
Size: 1946 679
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 745 780 2625
Size: 745 35 1845
Fragments arranged by size:
1845 745 35
DraI TTT'AAA
Cuts at: 0 791 1509 2625
Size: 791 718 1116
Fragments arranged by size:
1116 791 718
EaeI y'GGCC_r
Cuts at: 0 2268 2625
Size: 2268 357
EarI CTCTTCn'nnn_
Cuts at: 0 881 2625
Size: 881 1744
EcoICRI GAG'CTC
Cuts at: 0 1222 2625
Size: 1222 1403
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 1087 1541 1648 2267 2477 2625
Size: 217 870 454 107 619 210 148
Fragments arranged by size:
870 619 454 217 210 148 107
EcoO109I rG'GnC_Cy
Cuts at: 0 1201 2625
Size: 1201 1424
EcoRI G'AATT_C
Cuts at: 0 572 2158 2625
Size: 572 1586 467
Fragments arranged by size:
1586 572 467
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 189 2625
Size: 189 2436
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1651 1683 1764 1796 2625
Size: 1651 32 81 32 829
Fragments arranged by size:
1651 829 81 32 32
HincII GTy'rAC
Cuts at: 0 14 2625
Size: 14 2611
KpnI G_GTAC'C
Cuts at: 0 2146 2625
Size: 2146 479
MscI TGG'CCA
Cuts at: 0 2270 2625
Size: 2270 355
MslI CAynn'nnrTG
Cuts at: 0 265 856 989 1088 2276 2625
Size: 265 591 133 99 1188 349
Fragments arranged by size:
1188 591 349 265 133 99
MspA1I CmG'CkG
Cuts at: 0 158 1074 1755 2625
Size: 158 916 681 870
Fragments arranged by size:
916 870 681 158
NcoI C'CATG_G
Cuts at: 0 1116 1446 1605 2625
Size: 1116 330 159 1020
Fragments arranged by size:
1116 1020 330 159
NsiI A_TGCA'T
Cuts at: 0 183 2625
Size: 183 2442
NspI r_CATG'y
Cuts at: 0 224 569 861 988 1194 2209 2625
Size: 224 345 292 127 206 1015 416
Fragments arranged by size:
1015 416 345 292 224 206 127
PasI CC'CwG_GG
Cuts at: 0 2392 2625
Size: 2392 233
PciI A'CATG_T
Cuts at: 0 565 1190 2625
Size: 565 625 1435
Fragments arranged by size:
1435 625 565
PflMI CCAn_nnn'nTGG
Cuts at: 0 1122 1597 2625
Size: 1122 475 1028
Fragments arranged by size:
1122 1028 475
PshAI GACnn'nnGTC
Cuts at: 0 1865 2625
Size: 1865 760
PsiI TTA'TAA
Cuts at: 0 1454 2200 2258 2625
Size: 1454 746 58 367
Fragments arranged by size:
1454 746 367 58
PstI C_TGCA'G
Cuts at: 0 163 1850 2625
Size: 163 1687 775
Fragments arranged by size:
1687 775 163
PvuII CAG'CTG
Cuts at: 0 158 1755 2625
Size: 158 1597 870
Fragments arranged by size:
1597 870 158
SacI G_AGCT'C
Cuts at: 0 1224 2625
Size: 1224 1401
ScaI AGT'ACT
Cuts at: 0 1749 2625
Size: 1749 876
SfcI C'TryA_G
Cuts at: 0 159 1846 2625
Size: 159 1687 779
Fragments arranged by size:
1687 779 159
SmaI CCC'GGG
Cuts at: 0 2512 2625
Size: 2512 113
SmlI C'TyrA_G
Cuts at: 0 52 2625
Size: 52 2573
SspI AAT'ATT
Cuts at: 0 517 1542 2249 2625
Size: 517 1025 707 376
Fragments arranged by size:
1025 707 517 376
StuI AGG'CCT
Cuts at: 0 119 1064 1836 2625
Size: 119 945 772 789
Fragments arranged by size:
945 789 772 119
StyI C'CwwG_G
Cuts at: 0 399 1116 1446 1605 1837 2625
Size: 399 717 330 159 232 788
Fragments arranged by size:
788 717 399 330 232 159
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 245 1801 2080 2384 2625
Size: 245 1556 279 304 241
Fragments arranged by size:
1556 304 279 245 241
TatI w'GTAC_w
Cuts at: 0 1747 2625
Size: 1747 878
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 809 841 1381 1413 2216 2248 2304 2336
Size: 809 32 540 32 803 32 56 32
Cuts at: 2336 2625
Size: 289
Fragments arranged by size:
809 803 540 289 56 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2474 2625
Size: 2474 151
XmaI C'CCGG_G
Cuts at: 0 2510 2625
Size: 2510 115
XmnI GAAnn'nnTTC
Cuts at: 0 576 2625
Size: 576 2049
Enzymes that do cut:
AarI Acc65I AcuI AflII AflIII AleI AlwNI ApoI
AvaI AvrII BaeI BanI BanII BbsI BcgI BciVI
BclI BfrBI Bme1580I BmrI BpmI BsaI BsaBI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrDI BsrFI BstXI BtgI BtgZI CspCI DraI
EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI FalI Hin4I
HincII KpnI MscI MslI MspA1I NcoI NsiI NspI
PasI PciI PflMI PshAI PsiI PstI PvuII SacI
ScaI SfcI SmaI SmlI SspI StuI StyI TaqII
TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AgeI AhdI AlfI AloI
ApaI ApaLI AscI AseI AsiSI BamHI BbeI BbvCI
BglI BglII BlpI BmgBI BmtI BplI Bpu10I BpuEI
BsaAI BsaHI BsiEI BsiWI BsmBI BspEI BsrBI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtsI ClaI DraIII DrdI EagI EciI EcoNI EcoRV
FseI FspI FspAI HaeII HindIII HpaI KasI MfeI
MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PfoI PmeI PmlI PpiI PpuMI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SwaI Tth111I XbaI XhoI ZraI