(Circular) (Six-base) MAPSORT of: pFN21AB9376.seq Check: 5063 from: 1 to: 5938 pFN21AB9376 5938 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3748 278 Size: 53 83 186 936 2212 2468 Fragments arranged by size: 2468 2212 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3281 1937 Size: 1344 4594 Acc65I G'GTAC_C Cuts at: 3259 3259 Size: 5938 AclI AA'CG_TT Cuts at: 1563 4065 4438 1563 Size: 2502 373 3063 Fragments arranged by size: 3063 2502 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2412 2736 2912 3120 4001 5385 5653 1632 Size: 780 324 176 208 881 1384 268 1917 Fragments arranged by size: 1917 1384 881 780 324 268 208 176 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5741 AflIII A'CryG_T Cuts at: 1203 4837 5814 1203 Size: 3634 977 1327 Fragments arranged by size: 3634 1327 977 AgeI A'CCGG_T Cuts at: 4769 4769 Size: 5938 AhdI GACnn_n'nnGTC Cuts at: 4667 4667 Size: 5938 AleI CACnn'nnGTG Cuts at: 1166 2615 1166 Size: 1449 4489 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5938 AlwNI CAG_nnn'CTG Cuts at: 2663 2964 3023 3681 5253 2663 Size: 301 59 658 1572 3348 Fragments arranged by size: 3348 1572 658 301 59 ApaLI G'TGCA_C Cuts at: 3995 5151 3995 Size: 1156 4782 ApoI r'AATT_y Cuts at: 1493 2149 2305 2403 2436 3247 3473 3625 5734 Size: 656 156 98 33 811 226 152 2109 Cuts at: 5734 1493 Size: 1697 Fragments arranged by size: 2109 1697 811 656 226 156 152 98 33 AseI AT'TA_AT Cuts at: 160 1032 4492 160 Size: 872 3460 1606 Fragments arranged by size: 3460 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5938 AvaI C'yCGr_G Cuts at: 1870 1943 3263 1870 Size: 73 1320 4545 Fragments arranged by size: 4545 1320 73 BamHI G'GATC_C Cuts at: 3268 3635 3268 Size: 367 5571 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2596 3259 4714 618 Size: 325 217 627 809 663 1455 1842 Fragments arranged by size: 1842 1455 809 663 627 325 217 BanII G_rGCy'C Cuts at: 1391 2039 2046 3257 1391 Size: 648 7 1211 4072 Fragments arranged by size: 4072 1211 648 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4967 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4129 4163 1903 Size: 34 2192 34 3678 Fragments arranged by size: 3678 2192 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3830 5040 3830 Size: 1210 4728 BclI T'GATC_A Cuts at: 1548 2248 1548 Size: 700 5238 BfrBI ATG'CAT Cuts at: 2087 2087 Size: 5938 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4549 136 Size: 107 122 71 631 605 2877 1525 Fragments arranged by size: 2877 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5933 5933 Size: 5938 BlpI GC'TnA_GC Cuts at: 3355 3355 Size: 5938 Bme1580I G_kGCm'C Cuts at: 1790 3999 5155 1790 Size: 2209 1156 2573 Fragments arranged by size: 2573 2209 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5938 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2518 2974 3669 Size: 416 53 83 399 24 1101 456 695 Cuts at: 3669 4627 442 Size: 958 1753 Fragments arranged by size: 1753 1101 958 695 456 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5938 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3045 4598 1239 Size: 1806 1553 2579 Fragments arranged by size: 2579 1806 1553 Bpu10I CC'TnA_GC Cuts at: 2038 2038 Size: 5938 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4063 4928 5226 4063 Size: 865 298 4775 Fragments arranged by size: 4775 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4601 882 Size: 617 3102 2219 Fragments arranged by size: 3102 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3729 493 Size: 711 2525 2702 Fragments arranged by size: 2702 2525 711 BsaBI GATnn'nnATC Cuts at: 5932 5932 Size: 5938 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3745 4127 4762 275 Size: 53 83 186 936 2212 382 635 1451 Fragments arranged by size: 2212 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4371 4769 5043 5190 5601 4371 Size: 398 274 147 411 4708 Fragments arranged by size: 4708 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2626 2656 1178 Size: 30 165 30 1223 30 4460 Fragments arranged by size: 4460 1223 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5938 BseYI C'CCAG_C Cuts at: 5141 5637 5141 Size: 496 5442 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2294 1163 Size: 573 558 4807 Fragments arranged by size: 4807 573 558 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3388 4149 5177 1540 Size: 275 186 1387 761 1028 2301 Fragments arranged by size: 2301 1387 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2555 3999 4084 5155 2555 Size: 1444 85 1071 3338 Fragments arranged by size: 3338 1444 1071 85 BsmI GAATG_Cn' Cuts at: 3449 3542 3449 Size: 93 5845 BsmBI CGTCTCn'nnnn_ Cuts at: 2350 2350 Size: 5938 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2039 2046 2555 3257 3999 4084 5155 Size: 399 249 7 509 702 742 85 1071 Cuts at: 5155 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 702 509 399 249 85 7 BspEI T'CCGG_A Cuts at: 5601 5601 Size: 5938 BspHI T'CATG_A Cuts at: 3827 5557 3827 Size: 1730 4208 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3293 844 Size: 2449 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3825 1394 Size: 429 2002 3507 Fragments arranged by size: 3507 2002 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2695 2940 4433 4607 65 Size: 1183 1447 245 1493 174 1396 Fragments arranged by size: 1493 1447 1396 1183 245 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4582 4769 1700 Size: 110 2772 187 2869 Fragments arranged by size: 2869 2772 187 110 BsrGI T'GTAC_A Cuts at: 96 2521 96 Size: 2425 3513 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5938 BssSI C'ACGA_G Cuts at: 3998 5010 3998 Size: 1012 4926 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5938 BstBI TT'CG_AA Cuts at: 4742 4742 Size: 5938 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5938 BstYI r'GATC_y Cuts at: 1972 3268 3635 4020 4037 5489 5575 5587 5933 Size: 1296 367 385 17 1452 86 12 346 Cuts at: 5933 1972 Size: 1977 Fragments arranged by size: 1977 1452 1296 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2209 2712 513 Size: 552 939 205 503 3739 Fragments arranged by size: 3739 939 552 503 205 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5199 Fragments arranged by size: 5199 683 56 BtsI GCAGTG_nn' Cuts at: 2277 2808 3153 3454 4248 4268 2277 Size: 531 345 301 794 20 3947 Fragments arranged by size: 3947 794 531 345 301 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5938 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5903 DraI TTT'AAA Cuts at: 3242 3242 Size: 5938 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4945 809 Size: 507 3629 1802 Fragments arranged by size: 3629 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2427 3385 4274 Size: 54 1425 50 275 18 597 958 889 Cuts at: 4274 5903 8 Size: 1629 43 Fragments arranged by size: 1629 1425 958 889 597 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3385 1537 Size: 275 1573 4090 Fragments arranged by size: 4090 1573 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2027 3868 1353 Size: 674 1841 3423 Fragments arranged by size: 3423 1841 674 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2006 4525 4899 5045 387 Size: 1064 138 417 2519 374 146 1280 Fragments arranged by size: 2519 1280 1064 417 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2412 2736 2912 3045 3120 4001 4598 Size: 393 780 324 176 133 75 881 597 Cuts at: 4598 5385 5653 1239 Size: 787 268 1524 Fragments arranged by size: 1524 881 787 780 597 393 324 268 176 133 75 EcoRI G'AATT_C Cuts at: 2403 2436 3247 3625 2403 Size: 33 811 378 4716 Fragments arranged by size: 4716 811 378 33 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2423 2423 Size: 5938 FspI TGC'GCA Cuts at: 4444 4444 Size: 5938 HaeII r_GCGC'y Cuts at: 1678 5085 1678 Size: 3407 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2330 2362 4579 4611 4653 Size: 32 135 32 758 32 2217 32 42 Cuts at: 4653 4685 1373 Size: 32 2626 Fragments arranged by size: 2626 2217 758 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3282 3528 669 Size: 988 281 1344 246 3079 Fragments arranged by size: 3079 1344 988 281 246 HindIII A'AGCT_T Cuts at: 748 2526 748 Size: 1778 4160 HpaI GTT'AAC Cuts at: 3528 3528 Size: 5938 KpnI G_GTAC'C Cuts at: 3263 3263 Size: 5938 MfeI C'AATT_G Cuts at: 2334 3537 2334 Size: 1203 4735 MluI A'CGCG_T Cuts at: 5814 5814 Size: 5938 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2524 5052 5236 1305 Size: 1219 2528 184 2007 Fragments arranged by size: 2528 2007 1219 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2429 10 Size: 54 1425 343 597 3519 Fragments arranged by size: 3519 1425 597 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2615 2909 3896 4255 4414 518 Size: 648 1449 294 987 359 159 2042 Fragments arranged by size: 2042 1449 987 648 359 294 159 MspA1I CmG'CkG Cuts at: 1606 2725 2796 3098 3354 4031 5179 5424 1606 Size: 1119 71 302 256 677 1148 245 2120 Fragments arranged by size: 2120 1148 1119 677 302 256 245 71 NcoI C'CATG_G Cuts at: 513 1065 2004 2209 2712 513 Size: 552 939 205 503 3739 Fragments arranged by size: 3739 939 552 503 205 NdeI CA'TA_TG Cuts at: 387 387 Size: 5938 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5938 NotI GC'GGCC_GC Cuts at: 3385 3385 Size: 5938 NsiI A_TGCA'T Cuts at: 2089 2089 Size: 5938 NspI r_CATG'y Cuts at: 1132 1229 2914 3296 4841 1132 Size: 97 1685 382 1545 2229 Fragments arranged by size: 2229 1685 1545 382 97 PasI CC'CwG_GG Cuts at: 1348 2977 1348 Size: 1629 4309 PciI A'CATG_T Cuts at: 4837 4837 Size: 5938 PflMI CCAn_nnn'nTGG Cuts at: 1108 2663 1108 Size: 1555 4383 PfoI T'CCnGG_A Cuts at: 2294 2294 Size: 5938 PmeI GTTT'AAAC Cuts at: 3242 3242 Size: 5938 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4007 4039 5520 5552 4007 Size: 32 1481 32 4393 Fragments arranged by size: 4393 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5938 PsiI TTA'TAA Cuts at: 2187 3173 3508 2187 Size: 986 335 4617 Fragments arranged by size: 4617 986 335 PstI C_TGCA'G Cuts at: 830 2724 3290 830 Size: 1894 566 3478 Fragments arranged by size: 3478 1894 566 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5938 PvuII CAG'CTG Cuts at: 2725 2796 3098 2725 Size: 71 302 5565 Fragments arranged by size: 5565 302 71 SalI G'TCGA_C Cuts at: 1936 3280 1936 Size: 1344 4594 SbfI CC_TGCA'GG Cuts at: 3290 3290 Size: 5938 ScaI AGT'ACT Cuts at: 4186 5898 4186 Size: 1712 4226 SfcI C'TryA_G Cuts at: 826 1045 2720 3093 3286 4421 5102 5293 826 Size: 219 1675 373 193 1135 681 191 1471 Fragments arranged by size: 1675 1471 1135 681 373 219 193 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5938 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5938 SmaI CCC'GGG Cuts at: 1872 3265 1872 Size: 1393 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4042 4943 5205 820 Size: 197 926 2099 901 262 1553 Fragments arranged by size: 2099 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3729 493 Size: 3236 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5938 SphI G_CATG'C Cuts at: 1132 3296 1132 Size: 2164 3774 SspI AAT'ATT Cuts at: 5 52 3862 5 Size: 47 3810 2081 Fragments arranged by size: 3810 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5938 StyI C'CwwG_G Cuts at: 513 1065 2004 2209 2313 2712 3159 3377 513 Size: 552 939 205 104 399 447 218 3074 Fragments arranged by size: 3074 939 552 447 399 218 205 104 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2614 3140 3965 3982 4135 4320 4765 Size: 1255 1034 526 825 17 153 185 445 Cuts at: 4765 325 Size: 1498 Fragments arranged by size: 1498 1255 1034 825 526 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2521 4184 5896 Size: 274 80 33 51 1443 544 1663 1712 Cuts at: 5896 96 Size: 138 Fragments arranged by size: 1712 1663 1443 544 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2365 2397 5898 5930 2365 Size: 32 3501 32 2373 Fragments arranged by size: 3501 2373 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2342 2855 1316 Size: 1026 513 4399 Fragments arranged by size: 4399 1026 513 XbaI T'CTAG_A Cuts at: 2848 3274 2848 Size: 426 5512 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5938 XmaI C'CCGG_G Cuts at: 1870 3263 1870 Size: 1393 4545 XmnI GAAnn'nnTTC Cuts at: 2671 3088 4067 2671 Size: 417 979 4542 Fragments arranged by size: 4542 979 417 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3746 276 Size: 53 83 186 936 2212 2468 Fragments arranged by size: 2468 2212 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI