(Circular) (Six-base) MAPSORT of: pFN21AA1909.seq Check: 594 from: 1 to: 8518 pFN21AA1909 8518 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2560 3695 4393 2560 Size: 1135 698 6685 Fragments arranged by size: 6685 1135 698 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2583 5133 6328 278 Size: 53 83 186 936 1047 2550 1195 2468 Fragments arranged by size: 2550 2468 1195 1047 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5133 5861 1937 Size: 3196 728 4594 Fragments arranged by size: 4594 3196 728 Acc65I G'GTAC_C Cuts at: 5839 5839 Size: 8518 AclI AA'CG_TT Cuts at: 1563 6645 7018 1563 Size: 5082 373 3063 Fragments arranged by size: 5082 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2969 3740 4240 6581 7965 8233 1632 Size: 1337 771 500 2341 1384 268 1917 Fragments arranged by size: 2341 1917 1384 1337 771 500 268 AfeI AGC'GCT Cuts at: 3984 4619 3984 Size: 635 7883 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8321 AflIII A'CryG_T Cuts at: 1203 7417 8394 1203 Size: 6214 977 1327 Fragments arranged by size: 6214 1327 977 AgeI A'CCGG_T Cuts at: 7349 7349 Size: 8518 AhdI GACnn_n'nnGTC Cuts at: 2172 4045 7247 2172 Size: 1873 3202 3443 Fragments arranged by size: 3443 3202 1873 AleI CACnn'nnGTG Cuts at: 1166 5525 1166 Size: 4359 4159 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3888 4734 4970 1184 Size: 2704 846 236 4732 Fragments arranged by size: 4732 2704 846 236 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5331 5363 5331 Size: 32 8486 AlwNI CAG_nnn'CTG Cuts at: 2219 2575 2593 3049 3376 4135 4852 4894 5694 Size: 356 18 456 327 759 717 42 800 Cuts at: 5694 5715 6261 7833 2219 Size: 21 546 1572 2904 Fragments arranged by size: 2904 1572 800 759 717 546 456 356 327 42 21 18 ApaI G_GGCC'C Cuts at: 2287 3606 4019 2287 Size: 1319 413 6786 Fragments arranged by size: 6786 1319 413 ApaLI G'TGCA_C Cuts at: 2957 3335 6575 7731 2957 Size: 378 3240 1156 3744 Fragments arranged by size: 3744 3240 1156 378 ApoI r'AATT_y Cuts at: 1493 2963 3097 4990 5827 6053 6205 8314 1493 Size: 1470 134 1893 837 226 152 2109 1697 Fragments arranged by size: 2109 1893 1697 1470 837 226 152 134 AseI AT'TA_AT Cuts at: 160 1032 7072 160 Size: 872 6040 1606 Fragments arranged by size: 6040 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8518 AvaI C'yCGr_G Cuts at: 1870 1943 2054 2148 2513 2628 3698 4366 5665 Size: 73 111 94 365 115 1070 668 1299 Cuts at: 5665 5843 1870 Size: 178 4545 Fragments arranged by size: 4545 1299 1070 668 365 178 115 111 94 73 AvrII C'CTAG_G Cuts at: 2344 2344 Size: 8518 BamHI G'GATC_C Cuts at: 5307 5848 6215 5307 Size: 541 367 7610 Fragments arranged by size: 7610 541 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2263 2560 3233 3894 3938 Size: 325 217 627 476 297 673 661 44 Cuts at: 3938 5756 5839 7294 618 Size: 1818 83 1455 1842 Fragments arranged by size: 1842 1818 1455 673 661 627 476 325 297 217 83 44 BanII G_rGCy'C Cuts at: 1391 2287 2945 3606 3857 4011 4019 4125 4331 Size: 896 658 661 251 154 8 106 206 Cuts at: 4331 4374 4905 5446 5681 5785 5837 1391 Size: 43 531 541 235 104 52 4072 Fragments arranged by size: 4072 896 661 658 541 531 251 235 206 154 106 104 52 43 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2418 3204 3282 4246 5088 928 Size: 971 519 786 78 964 842 4358 Fragments arranged by size: 4358 971 964 842 786 519 78 BbvCI CC'TCA_GC Cuts at: 2428 3251 4203 4383 2428 Size: 823 952 180 6563 Fragments arranged by size: 6563 952 823 180 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2477 2511 6709 6743 1903 Size: 34 540 34 4198 34 3678 Fragments arranged by size: 4198 3678 540 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4472 6410 7620 4472 Size: 1938 1210 5370 Fragments arranged by size: 5370 1938 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8518 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2500 3881 4085 Size: 107 122 71 631 605 828 1381 204 Cuts at: 4085 7129 136 Size: 3044 1525 Fragments arranged by size: 3044 1525 1381 828 631 605 204 122 107 71 BglII A'GATC_T Cuts at: 2243 8513 2243 Size: 6270 2248 BlpI GC'TnA_GC Cuts at: 2706 4727 5798 5935 2706 Size: 2021 1071 137 5289 Fragments arranged by size: 5289 2021 1071 137 Bme1580I G_kGCm'C Cuts at: 1790 2266 2287 2565 2961 3236 3339 3606 3877 Size: 476 21 278 396 275 103 267 271 Cuts at: 3877 4019 5498 5759 6579 7735 1790 Size: 142 1479 261 820 1156 2573 Fragments arranged by size: 2573 1479 1156 820 476 396 278 275 271 267 261 142 103 21 BmgBI CAC'GTC Cuts at: 1318 4987 1318 Size: 3669 4849 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2115 2375 6249 Size: 416 53 83 399 24 698 260 3874 Cuts at: 6249 7207 442 Size: 958 1753 Fragments arranged by size: 3874 1753 958 698 416 399 260 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8518 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2155 3691 4120 2155 Size: 1536 429 6553 Fragments arranged by size: 6553 1536 429 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2103 2260 2311 3245 3314 3353 3398 4115 Size: 864 157 51 934 69 39 45 717 Cuts at: 4115 4289 4634 4829 5635 7178 1239 Size: 174 345 195 806 1543 2579 Fragments arranged by size: 2579 1543 934 864 806 717 345 195 174 157 69 51 45 39 Bpu10I CC'TnA_GC Cuts at: 2428 3251 3506 4203 4383 4474 2428 Size: 823 255 697 180 91 6472 Fragments arranged by size: 6472 823 697 255 180 91 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4841 5221 6643 7508 7806 4841 Size: 380 1422 865 298 5553 Fragments arranged by size: 5553 1422 865 380 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2148 2625 5400 7181 882 Size: 617 649 477 2775 1781 2219 Fragments arranged by size: 2775 2219 1781 649 617 477 BsaAI yAC'GTr Cuts at: 493 1204 4687 6309 493 Size: 711 3483 1622 2702 Fragments arranged by size: 3483 2702 1622 711 BsaBI GATnn'nnATC Cuts at: 8512 8512 Size: 8518 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2580 3398 4968 5130 Size: 53 83 186 936 1047 818 1570 162 Cuts at: 5130 6325 6707 7342 275 Size: 1195 382 635 1451 Fragments arranged by size: 1570 1451 1195 1047 936 818 635 382 186 162 83 53 BsaWI w'CCGG_w Cuts at: 5409 6951 7349 7623 7770 8181 5409 Size: 1542 398 274 147 411 5746 Fragments arranged by size: 5746 1542 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3383 3413 4035 4065 4778 Size: 30 165 30 1980 30 622 30 713 Cuts at: 4778 4808 1178 Size: 30 4888 Fragments arranged by size: 4888 1980 713 622 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2060 3268 3344 3619 3667 3904 4043 4400 Size: 874 1208 76 275 48 237 139 357 Cuts at: 4400 5524 5584 5605 1186 Size: 1124 60 21 4099 Fragments arranged by size: 4099 1208 1124 874 357 275 237 139 76 60 48 21 BseYI C'CCAG_C Cuts at: 2198 2474 2552 3830 3917 4894 5632 5656 7721 Size: 276 78 1278 87 977 738 24 2065 Cuts at: 7721 8217 2198 Size: 496 2499 Fragments arranged by size: 2499 2065 1278 977 738 496 276 87 78 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2383 2675 3553 4835 5445 5475 1163 Size: 573 647 292 878 1282 610 30 4206 Fragments arranged by size: 4206 1282 878 647 610 573 292 30 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3319 5163 5470 5968 6729 7757 Size: 275 186 1318 1844 307 498 761 1028 Cuts at: 7757 1540 Size: 2301 Fragments arranged by size: 2301 1844 1318 1028 761 498 307 275 186 BsiHKAI G_wGCw'C Cuts at: 2945 2961 3339 3384 4033 4905 6579 6664 7735 Size: 16 378 45 649 872 1674 85 1071 Cuts at: 7735 2945 Size: 3728 Fragments arranged by size: 3728 1674 1071 872 649 378 85 45 16 BsmI GAATG_Cn' Cuts at: 6029 6122 6029 Size: 93 8425 BsmBI CGTCTCn'nnnn_ Cuts at: 2821 3167 3371 3575 5744 2821 Size: 346 204 204 2169 5595 Fragments arranged by size: 5595 2169 346 204 204 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2266 2287 2565 2945 2961 3236 3339 Size: 399 476 21 278 380 16 275 103 Cuts at: 3339 3384 3606 3857 3877 4011 4019 4033 4125 Size: 45 222 251 20 134 8 14 92 Cuts at: 4125 4331 4374 4905 5446 5498 5681 5759 5785 Size: 206 43 531 541 52 183 78 26 Cuts at: 5785 5837 6579 6664 7735 1391 Size: 52 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 541 531 476 399 380 278 275 251 222 206 183 134 103 92 85 78 52 52 45 43 26 21 20 16 14 8 BspEI T'CCGG_A Cuts at: 8181 8181 Size: 8518 BspHI T'CATG_A Cuts at: 6407 8137 6407 Size: 1730 6788 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2560 2647 3695 3958 4393 4809 4860 4962 Size: 1716 87 1048 263 435 416 51 102 Cuts at: 4962 5873 844 Size: 911 3489 Fragments arranged by size: 3489 1716 1048 911 435 416 263 102 87 51 BsrBI CCG'CTC Cuts at: 1394 1823 3564 6405 1394 Size: 429 1741 2841 3507 Fragments arranged by size: 3507 2841 1741 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2461 4954 7013 7187 65 Size: 1183 1213 2493 2059 174 1396 Fragments arranged by size: 2493 2059 1396 1213 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7162 7349 1700 Size: 110 5352 187 2869 Fragments arranged by size: 5352 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 5139 96 Size: 5043 3475 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8518 BssSI C'ACGA_G Cuts at: 6578 7590 6578 Size: 1012 7506 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8518 BstBI TT'CG_AA Cuts at: 7322 7322 Size: 8518 BstEII G'GTnAC_C Cuts at: 1184 4273 1184 Size: 3089 5429 BstXI CCAn_nnnn'nTGG Cuts at: 2032 2032 Size: 8518 BstYI r'GATC_y Cuts at: 1972 2243 3030 5307 5848 6215 6600 6617 8069 Size: 271 787 2277 541 367 385 17 1452 Cuts at: 8069 8155 8167 8513 1972 Size: 86 12 346 1977 Fragments arranged by size: 2277 1977 1452 787 541 385 367 346 271 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4055 4055 Size: 8518 BtgI C'CryG_G Cuts at: 513 1065 2004 3019 3513 3636 3743 4083 4641 Size: 552 939 1015 494 123 107 340 558 Cuts at: 4641 4735 4962 4975 5364 513 Size: 94 227 13 389 3667 Fragments arranged by size: 3667 1015 939 558 552 494 389 340 227 123 107 94 13 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7779 Fragments arranged by size: 7779 683 56 BtsI GCAGTG_nn' Cuts at: 2655 3203 3720 4627 5433 5463 5775 6034 6828 Size: 548 517 907 806 30 312 259 794 Cuts at: 6828 6848 2655 Size: 20 4325 Fragments arranged by size: 4325 907 806 794 548 517 312 259 30 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8518 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2507 2542 3746 3781 529 Size: 35 1943 35 1204 35 5266 Fragments arranged by size: 5266 1943 1204 35 35 35 DraI TTT'AAA Cuts at: 5822 5822 Size: 8518 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3565 5486 7525 809 Size: 507 2249 1921 2039 1802 Fragments arranged by size: 2249 2039 1921 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2528 3549 4137 Size: 54 1425 50 275 18 698 1021 588 Cuts at: 4137 4434 4638 4824 4959 5160 5965 6854 8483 Size: 297 204 186 135 201 805 889 1629 Cuts at: 8483 8 Size: 43 Fragments arranged by size: 1629 1425 1021 889 805 698 588 297 275 204 201 186 135 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5160 5965 1537 Size: 275 3348 805 4090 Fragments arranged by size: 4090 3348 805 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2238 3255 3795 4662 5602 6448 1353 Size: 885 1017 540 867 940 846 3423 Fragments arranged by size: 3423 1017 940 885 867 846 540 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2242 7105 7479 7625 387 Size: 1064 138 653 4863 374 146 1280 Fragments arranged by size: 4863 1280 1064 653 374 146 138 EcoICRI GAG'CTC Cuts at: 2943 4903 2943 Size: 1960 6558 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2103 2260 2311 2969 3245 3314 3353 Size: 393 471 157 51 658 276 69 39 Cuts at: 3353 3398 3740 4115 4240 4289 4634 4829 5635 Size: 45 342 375 125 49 345 195 806 Cuts at: 5635 6581 7178 7965 8233 1239 Size: 946 597 787 268 1524 Fragments arranged by size: 1524 946 806 787 658 597 471 393 375 345 342 276 268 195 157 125 69 51 49 45 39 EcoNI CCTnn'n_nnAGG Cuts at: 2257 3812 4856 4880 2257 Size: 1555 1044 24 5895 Fragments arranged by size: 5895 1555 1044 24 EcoO109I rG'GnC_Cy Cuts at: 2050 2059 2095 2124 2283 2364 2390 2625 2761 Size: 9 36 29 159 81 26 235 136 Cuts at: 2761 2824 3502 3602 3603 3805 4039 4060 4264 Size: 63 678 100 1 202 234 21 204 Cuts at: 4264 4531 4849 5473 2050 Size: 267 318 624 5095 Fragments arranged by size: 5095 678 624 318 267 235 234 204 202 159 136 100 81 63 36 29 26 21 9 1 EcoRI G'AATT_C Cuts at: 3097 5827 6205 3097 Size: 2730 378 5410 Fragments arranged by size: 5410 2730 378 EcoRV GAT'ATC Cuts at: 5707 5707 Size: 8518 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2435 3824 5167 2435 Size: 1389 1343 5786 Fragments arranged by size: 5786 1389 1343 FspI TGC'GCA Cuts at: 7024 7024 Size: 8518 HaeII r_GCGC'y Cuts at: 1678 3513 3986 4621 7665 1678 Size: 1835 473 635 3044 2531 Fragments arranged by size: 3044 2531 1835 635 473 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2158 2190 2876 2908 3383 Size: 32 135 32 586 32 686 32 475 Cuts at: 3383 3410 3415 3442 3643 3675 4031 4063 4336 Size: 27 5 27 201 32 356 32 273 Cuts at: 4336 4368 4778 4810 5703 5735 7159 7191 7233 Size: 32 410 32 893 32 1424 32 42 Cuts at: 7233 7265 1373 Size: 32 2626 Fragments arranged by size: 2626 1424 893 686 586 475 410 356 273 201 135 42 32 32 32 32 32 32 32 32 32 32 32 27 27 5 HincII GTy'rAC Cuts at: 669 1657 1938 2971 5862 6108 669 Size: 988 281 1033 2891 246 3079 Fragments arranged by size: 3079 2891 1033 988 281 246 HindIII A'AGCT_T Cuts at: 748 3185 748 Size: 2437 6081 HpaI GTT'AAC Cuts at: 6108 6108 Size: 8518 KpnI G_GTAC'C Cuts at: 5843 5843 Size: 8518 MfeI C'AATT_G Cuts at: 6117 6117 Size: 8518 MluI A'CGCG_T Cuts at: 8394 8394 Size: 8518 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4695 7632 7816 1305 Size: 3390 2937 184 2007 Fragments arranged by size: 3390 2937 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2530 3551 4139 4826 4961 Size: 54 1425 343 698 1021 588 687 135 Cuts at: 4961 10 Size: 3567 Fragments arranged by size: 3567 1425 1021 698 687 588 343 135 54 MslI CAynn'nnrTG Cuts at: 518 1166 3870 4248 4947 5525 6476 6835 6994 Size: 648 2704 378 699 578 951 359 159 Cuts at: 6994 518 Size: 2042 Fragments arranged by size: 2704 2042 951 699 648 578 378 359 159 MspA1I CmG'CkG Cuts at: 1606 2478 2804 2976 2983 3376 3448 3594 4309 Size: 872 326 172 7 393 72 146 715 Cuts at: 4309 4765 4869 4936 4977 5011 5525 5934 6611 Size: 456 104 67 41 34 514 409 677 Cuts at: 6611 7759 8004 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 872 715 677 514 456 409 393 326 245 172 146 104 72 67 41 34 7 NcoI C'CATG_G Cuts at: 513 1065 2004 3019 4735 4962 5364 513 Size: 552 939 1015 1716 227 402 3667 Fragments arranged by size: 3667 1716 1015 939 552 402 227 NdeI CA'TA_TG Cuts at: 387 387 Size: 8518 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8518 NotI GC'GGCC_GC Cuts at: 5965 5965 Size: 8518 NspI r_CATG'y Cuts at: 1132 1229 4322 5069 5876 7421 1132 Size: 97 3093 747 807 1545 2229 Fragments arranged by size: 3093 2229 1545 807 747 97 PasI CC'CwG_GG Cuts at: 1348 2032 2621 3733 4012 4019 4221 4528 4882 Size: 684 589 1112 279 7 202 307 354 Cuts at: 4882 1348 Size: 4984 Fragments arranged by size: 4984 1112 684 589 354 307 279 202 7 PciI A'CATG_T Cuts at: 7417 7417 Size: 8518 PflMI CCAn_nnn'nTGG Cuts at: 1108 3376 4135 5113 5356 1108 Size: 2268 759 978 243 4270 Fragments arranged by size: 4270 2268 978 759 243 PfoI T'CCnGG_A Cuts at: 2212 2757 3192 3387 3405 3486 4605 4845 2212 Size: 545 435 195 18 81 1119 240 5885 Fragments arranged by size: 5885 1119 545 435 240 195 81 18 PmeI GTTT'AAAC Cuts at: 5822 5822 Size: 8518 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6587 6619 8100 8132 6587 Size: 32 1481 32 6973 Fragments arranged by size: 6973 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2059 2095 2124 2364 2390 2625 2761 4039 4849 Size: 36 29 240 26 235 136 1278 810 Cuts at: 4849 5473 2059 Size: 624 5104 Fragments arranged by size: 5104 1278 810 624 240 235 136 36 29 26 PshAI GACnn'nnGTC Cuts at: 1613 2583 5294 1613 Size: 970 2711 4837 Fragments arranged by size: 4837 2711 970 PsiI TTA'TAA Cuts at: 6088 6088 Size: 8518 PspOMI G'GGCC_C Cuts at: 2283 3602 4015 2283 Size: 1319 413 6786 Fragments arranged by size: 6786 1319 413 PstI C_TGCA'G Cuts at: 830 2072 2224 2296 2601 3688 3774 5770 5870 Size: 1242 152 72 305 1087 86 1996 100 Cuts at: 5870 830 Size: 3478 Fragments arranged by size: 3478 1996 1242 1087 305 152 100 86 72 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8518 PvuII CAG'CTG Cuts at: 2804 2976 2983 3376 3448 3594 4869 4936 5011 Size: 172 7 393 72 146 1275 67 75 Cuts at: 5011 2804 Size: 6311 Fragments arranged by size: 6311 1275 393 172 146 75 72 67 7 SacI G_AGCT'C Cuts at: 2945 4905 2945 Size: 1960 6558 SacII CC_GC'GG Cuts at: 4978 4978 Size: 8518 SalI G'TCGA_C Cuts at: 1936 5860 1936 Size: 3924 4594 SanDI GG'GwC_CC Cuts at: 2124 2364 2390 2625 4039 5473 2124 Size: 240 26 235 1414 1434 5169 Fragments arranged by size: 5169 1434 1414 240 235 26 SapI GCTCTTCn'nnn_ Cuts at: 3255 4662 3255 Size: 1407 7111 SbfI CC_TGCA'GG Cuts at: 5870 5870 Size: 8518 ScaI AGT'ACT Cuts at: 6766 8478 6766 Size: 1712 6806 SfcI C'TryA_G Cuts at: 826 1045 2068 2220 2292 2597 3013 3092 3443 Size: 219 1023 152 72 305 416 79 351 Cuts at: 3443 3684 3770 4284 4693 5766 5866 7001 7682 Size: 241 86 514 409 1073 100 1135 681 Cuts at: 7682 7873 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1073 1023 681 514 416 409 351 305 241 219 191 152 100 86 79 72 SfiI GGCCn_nnn'nGGCC Cuts at: 4085 4085 Size: 8518 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8518 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8518 SmaI CCC'GGG Cuts at: 1872 2150 3700 5667 5845 1872 Size: 278 1550 1967 178 4545 Fragments arranged by size: 4545 1967 1550 278 178 SmlI C'TyrA_G Cuts at: 820 1017 1943 4856 5200 6622 7523 7785 820 Size: 197 926 2913 344 1422 901 262 1553 Fragments arranged by size: 2913 1553 1422 926 901 344 262 197 SnaBI TAC'GTA Cuts at: 493 6309 493 Size: 5816 2702 SpeI A'CTAG_T Cuts at: 152 4247 152 Size: 4095 4423 SphI G_CATG'C Cuts at: 1132 4322 5876 1132 Size: 3190 1554 3774 Fragments arranged by size: 3774 3190 1554 SspI AAT'ATT Cuts at: 5 52 6442 5 Size: 47 6390 2081 Fragments arranged by size: 6390 2081 47 StuI AGG'CCT Cuts at: 1517 2332 2905 3692 1517 Size: 815 573 787 6343 Fragments arranged by size: 6343 815 787 573 StyI C'CwwG_G Cuts at: 513 1065 2004 2344 3019 4635 4735 4962 5364 Size: 552 939 340 675 1616 100 227 402 Cuts at: 5364 5957 513 Size: 593 3074 Fragments arranged by size: 3074 1616 939 675 593 552 402 340 227 100 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2212 2238 2281 3134 3333 4142 5306 Size: 1255 632 26 43 853 199 809 1164 Cuts at: 5306 5774 6545 6562 6715 6900 7345 325 Size: 468 771 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1164 853 809 771 632 468 445 199 185 153 43 26 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2727 4461 5139 Size: 274 80 33 51 1443 750 1734 678 Cuts at: 5139 6764 8476 96 Size: 1625 1712 138 Fragments arranged by size: 1734 1712 1625 1443 750 678 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2343 2375 3897 3929 4035 4067 8478 8510 2343 Size: 32 1522 32 106 32 4411 32 2351 Fragments arranged by size: 4411 2351 1522 106 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5471 1316 Size: 4155 4363 XbaI T'CTAG_A Cuts at: 2176 5854 2176 Size: 3678 4840 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2095 2572 4090 4132 4873 2095 Size: 477 1518 42 741 5740 Fragments arranged by size: 5740 1518 741 477 42 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8518 XmaI C'CCGG_G Cuts at: 1870 2148 3698 5665 5843 1870 Size: 278 1550 1967 178 4545 Fragments arranged by size: 4545 1967 1550 278 178 XmnI GAAnn'nnTTC Cuts at: 2415 3101 6647 2415 Size: 686 3546 4286 Fragments arranged by size: 4286 3546 686 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2581 5131 6326 276 Size: 53 83 186 936 1047 2550 1195 2468 Fragments arranged by size: 2550 2468 1195 1047 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BaeI BbeI BfrBI BsiWI BstZ17I DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SexAI SfoI SrfI SwaI