(Circular) (Six-base) MAPSORT of: pFN21AB0069.seq Check: 7631 from: 1 to: 12445 pFN21AB0069 12445 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2738 2738 Size: 12445 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 10255 278 Size: 53 83 186 936 8719 2468 Fragments arranged by size: 8719 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 9788 1937 Size: 7851 4594 Acc65I G'GTAC_C Cuts at: 9766 9766 Size: 12445 AclI AA'CG_TT Cuts at: 1563 10572 10945 1563 Size: 9009 373 3063 Fragments arranged by size: 9009 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2256 3146 3902 4088 4212 4251 4296 4538 Size: 624 890 756 186 124 39 45 242 Cuts at: 4538 4625 6375 6719 7139 8180 8951 10508 11892 Size: 87 1750 344 420 1041 771 1557 1384 Cuts at: 11892 12160 1632 Size: 268 1917 Fragments arranged by size: 1917 1750 1557 1384 1041 890 771 756 624 420 344 268 242 186 124 87 45 39 AflII C'TTAA_G Cuts at: 820 1017 6821 7403 820 Size: 197 5804 582 5862 Fragments arranged by size: 5862 5804 582 197 AflIII A'CryG_T Cuts at: 1203 2386 2909 4565 11344 12321 1203 Size: 1183 523 1656 6779 977 1327 Fragments arranged by size: 6779 1656 1327 1183 977 523 AgeI A'CCGG_T Cuts at: 11276 11276 Size: 12445 AhdI GACnn_n'nnGTC Cuts at: 11174 11174 Size: 12445 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 12445 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 9740 1184 Size: 8556 3889 AlwNI CAG_nnn'CTG Cuts at: 2744 3410 3808 6277 7237 8635 10188 11760 2744 Size: 666 398 2469 960 1398 1553 1572 3429 Fragments arranged by size: 3429 2469 1572 1553 1398 960 666 398 ApaLI G'TGCA_C Cuts at: 10502 11658 10502 Size: 1156 11289 ApoI r'AATT_y Cuts at: 1493 2375 2698 2874 2884 3370 3611 4382 4390 Size: 882 323 176 10 486 241 771 8 Cuts at: 4390 4496 4887 4909 4949 5413 5936 7366 7537 Size: 106 391 22 40 464 523 1430 171 Cuts at: 7537 7543 7767 7788 8027 8135 8219 8364 8411 Size: 6 224 21 239 108 84 145 47 Cuts at: 8411 8787 8803 9071 9306 9451 9529 9754 9980 Size: 376 16 268 235 145 78 225 226 Cuts at: 9980 10132 12241 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1430 882 771 523 486 464 391 376 323 268 241 239 235 226 225 224 176 171 152 145 145 108 106 84 78 47 40 22 21 16 10 8 6 AseI AT'TA_AT Cuts at: 160 1032 4869 5400 6408 10999 160 Size: 872 3837 531 1008 4591 1606 Fragments arranged by size: 4591 3837 1606 1008 872 531 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 12445 AvaI C'yCGr_G Cuts at: 1870 1943 6644 9770 1870 Size: 73 4701 3126 4545 Fragments arranged by size: 4701 4545 3126 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2919 2952 9165 9198 2919 Size: 33 6213 33 6166 Fragments arranged by size: 6213 6166 33 33 BamHI G'GATC_C Cuts at: 9775 10142 9775 Size: 367 12078 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3273 8708 9766 11221 618 Size: 325 217 627 1486 5435 1058 1455 1842 Fragments arranged by size: 5435 1842 1486 1455 1058 627 325 217 BanII G_rGCy'C Cuts at: 1391 3109 4239 9764 1391 Size: 1718 1130 5525 4072 Fragments arranged by size: 5525 4072 1718 1130 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3699 3789 4529 5144 5717 7334 8696 Size: 971 1800 90 740 615 573 1617 1362 Cuts at: 8696 928 Size: 4677 Fragments arranged by size: 4677 1800 1617 1362 971 740 615 573 90 BbvCI CC'TCA_GC Cuts at: 4205 9204 4205 Size: 4999 7446 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 10636 10670 1903 Size: 34 8699 34 3678 Fragments arranged by size: 8699 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2698 10337 11547 2698 Size: 7639 1210 3596 Fragments arranged by size: 7639 3596 1210 BclI T'GATC_A Cuts at: 1548 2808 4375 7975 1548 Size: 1260 1567 3600 6018 Fragments arranged by size: 6018 3600 1567 1260 BfrBI ATG'CAT Cuts at: 7114 7273 8836 7114 Size: 159 1563 10723 Fragments arranged by size: 10723 1563 159 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 11056 136 Size: 107 122 71 631 605 9384 1525 Fragments arranged by size: 9384 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6974 7684 9016 12440 6974 Size: 710 1332 3424 6979 Fragments arranged by size: 6979 3424 1332 710 BlpI GC'TnA_GC Cuts at: 8991 9862 8991 Size: 871 11574 Bme1580I G_kGCm'C Cuts at: 1790 8713 10506 11662 1790 Size: 6923 1793 1156 2573 Fragments arranged by size: 6923 2573 1793 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 12445 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5771 10176 11134 Size: 416 53 83 399 24 4354 4405 958 Cuts at: 11134 442 Size: 1753 Fragments arranged by size: 4405 4354 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 12445 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3523 7058 7667 9164 3523 Size: 3535 609 1497 6804 Fragments arranged by size: 6804 3535 1497 609 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2864 2916 2948 3896 4007 5873 7658 11105 Size: 1625 52 32 948 111 1866 1785 3447 Cuts at: 11105 1239 Size: 2579 Fragments arranged by size: 3447 2579 1866 1785 1625 948 111 52 32 Bpu10I CC'TnA_GC Cuts at: 3108 4205 5921 6252 9204 3108 Size: 1097 1716 331 2952 6349 Fragments arranged by size: 6349 2952 1716 1097 331 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2744 4460 4698 4800 4859 5495 6783 7028 7637 Size: 1716 238 102 59 636 1288 245 609 Cuts at: 7637 8894 9616 10570 11435 11733 2744 Size: 1257 722 954 865 298 3456 Fragments arranged by size: 3456 1716 1288 1257 954 865 722 636 609 298 245 238 102 59 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5571 6741 6884 7029 7068 7638 11108 Size: 617 4072 1170 143 145 39 570 3470 Cuts at: 11108 882 Size: 2219 Fragments arranged by size: 4072 3470 2219 1170 617 570 145 143 39 BsaAI yAC'GTr Cuts at: 493 1204 2387 2912 4568 10236 493 Size: 711 1183 525 1656 5668 2702 Fragments arranged by size: 5668 2702 1656 1183 711 525 BsaBI GATnn'nnATC Cuts at: 12439 12439 Size: 12445 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 10252 10634 11269 275 Size: 53 83 186 936 8719 382 635 1451 Fragments arranged by size: 8719 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 10878 11276 11550 11697 12108 10878 Size: 398 274 147 411 11215 Fragments arranged by size: 11215 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2862 2892 2914 2944 6842 Size: 30 165 30 1459 30 22 30 3898 Cuts at: 6842 6872 1178 Size: 30 6751 Fragments arranged by size: 6751 3898 1459 165 30 30 30 30 30 22 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3098 4457 7028 7106 7637 9700 1186 Size: 1912 1359 2571 78 531 2063 3931 Fragments arranged by size: 3931 2571 2063 1912 1359 531 78 BseYI C'CCAG_C Cuts at: 11648 12144 11648 Size: 496 11949 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2905 1163 Size: 573 1169 10703 Fragments arranged by size: 10703 1169 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2048 9895 10656 11684 1540 Size: 275 186 47 7847 761 1028 2301 Fragments arranged by size: 7847 2301 1028 761 275 186 47 BsiHKAI G_wGCw'C Cuts at: 4239 7128 7934 10506 10591 11662 4239 Size: 2889 806 2572 85 1071 5022 Fragments arranged by size: 5022 2889 2572 1071 806 85 BsmI GAATG_Cn' Cuts at: 9956 10049 9956 Size: 93 12352 BsmBI CGTCTCn'nnnn_ Cuts at: 3092 3092 Size: 12445 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3109 4239 7128 7934 8713 9764 10506 Size: 399 1319 1130 2889 806 779 1051 742 Cuts at: 10506 10591 11662 1391 Size: 85 1071 2174 Fragments arranged by size: 2889 2174 1319 1130 1071 1051 806 779 742 399 85 BspEI T'CCGG_A Cuts at: 12108 12108 Size: 12445 BspHI T'CATG_A Cuts at: 2335 5785 6229 7561 8148 8917 10334 12064 2335 Size: 3450 444 1332 587 769 1417 1730 2716 Fragments arranged by size: 3450 2716 1730 1417 1332 769 587 444 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2738 9800 844 Size: 1894 7062 3489 Fragments arranged by size: 7062 3489 1894 BsrBI CCG'CTC Cuts at: 1394 1823 10332 1394 Size: 429 8509 3507 Fragments arranged by size: 8509 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3120 10940 11114 65 Size: 1183 1872 7820 174 1396 Fragments arranged by size: 7820 1872 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 11089 11276 1700 Size: 110 9279 187 2869 Fragments arranged by size: 9279 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 5365 9250 96 Size: 5269 3885 3291 Fragments arranged by size: 5269 3885 3291 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 12445 BssSI C'ACGA_G Cuts at: 10505 11517 10505 Size: 1012 11433 BstAPI GCAn_nnn'nTGC Cuts at: 794 8708 794 Size: 7914 4531 BstBI TT'CG_AA Cuts at: 11249 11249 Size: 12445 BstEII G'GTnAC_C Cuts at: 1184 3208 1184 Size: 2024 10421 BstXI CCAn_nnnn'nTGG Cuts at: 9699 9699 Size: 12445 BstYI r'GATC_y Cuts at: 1972 2675 3064 5772 6501 6974 7234 7684 8323 Size: 703 389 2708 729 473 260 450 639 Cuts at: 8323 8347 9016 9538 9775 10142 10527 10544 11996 Size: 24 669 522 237 367 385 17 1452 Cuts at: 11996 12082 12094 12440 1972 Size: 86 12 346 1977 Fragments arranged by size: 2708 1977 1452 729 703 669 639 522 473 450 389 385 367 346 260 237 86 24 17 12 Bsu36I CC'TnA_GG Cuts at: 7001 9712 7001 Size: 2711 9734 BtgI C'CryG_G Cuts at: 513 1065 2004 2022 8130 513 Size: 552 939 18 6108 4828 Fragments arranged by size: 6108 4828 939 552 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 6581 487 Size: 683 56 5355 6351 Fragments arranged by size: 6351 5355 683 56 BtsI GCAGTG_nn' Cuts at: 2725 8807 9961 10755 10775 2725 Size: 6082 1154 794 20 4395 Fragments arranged by size: 6082 4395 1154 794 20 ClaI AT'CG_AT Cuts at: 1554 2217 1554 Size: 663 11782 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 12410 DraI TTT'AAA Cuts at: 5418 7542 8671 9749 5418 Size: 2124 1129 1078 8114 Fragments arranged by size: 8114 2124 1129 1078 DraIII CAC_nnn'GTG Cuts at: 3515 3515 Size: 12445 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2111 11452 809 Size: 507 795 9341 1802 Fragments arranged by size: 9341 1802 795 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2045 9892 Size: 54 1425 50 275 18 195 20 7847 Cuts at: 9892 10781 12410 8 Size: 889 1629 43 Fragments arranged by size: 7847 1629 1425 889 275 195 54 50 43 20 18 EagI C'GGCC_G Cuts at: 1537 1812 2045 9892 1537 Size: 275 233 7847 4090 Fragments arranged by size: 7847 4090 275 233 EarI CTCTTCn'nnn_ Cuts at: 1353 3220 3256 4227 5367 5763 5949 6351 7609 Size: 1867 36 971 1140 396 186 402 1258 Cuts at: 7609 7949 8454 10375 1353 Size: 340 505 1921 3423 Fragments arranged by size: 3423 1921 1867 1258 1140 971 505 402 396 340 186 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 11032 11406 11552 387 Size: 1064 138 435 9008 374 146 1280 Fragments arranged by size: 9008 1280 1064 435 374 146 138 EcoICRI GAG'CTC Cuts at: 4237 4237 Size: 12445 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2256 2864 2916 2948 3146 3896 3902 Size: 393 624 608 52 32 198 750 6 Cuts at: 3902 4007 4088 4212 4251 4296 4538 4625 5873 Size: 105 81 124 39 45 242 87 1248 Cuts at: 5873 6375 6719 7139 7658 8180 8951 10508 11105 Size: 502 344 420 519 522 771 1557 597 Cuts at: 11105 11892 12160 1239 Size: 787 268 1524 Fragments arranged by size: 1557 1524 1248 787 771 750 624 608 597 522 519 502 420 393 344 268 242 198 124 105 87 81 52 45 39 32 6 EcoO109I rG'GnC_Cy Cuts at: 5280 6730 8632 9695 5280 Size: 1450 1902 1063 8030 Fragments arranged by size: 8030 1902 1450 1063 EcoRI G'AATT_C Cuts at: 2874 2884 4382 8027 8135 9451 9754 10132 2874 Size: 10 1498 3645 108 1316 303 378 5187 Fragments arranged by size: 5187 3645 1498 1316 378 303 108 10 EcoRV GAT'ATC Cuts at: 9186 9186 Size: 12445 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2087 3176 3285 4328 5288 6026 6305 6380 8713 Size: 1089 109 1043 960 738 279 75 2333 Cuts at: 8713 2087 Size: 5819 Fragments arranged by size: 5819 2333 1089 1043 960 738 279 109 75 FspI TGC'GCA Cuts at: 10951 10951 Size: 12445 HaeII r_GCGC'y Cuts at: 1678 2745 11592 1678 Size: 1067 8847 2531 Fragments arranged by size: 8847 2531 1067 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2460 2492 2793 2825 3091 Size: 32 135 32 888 32 301 32 266 Cuts at: 3091 3123 4084 4116 4444 4476 5948 5950 5980 Size: 32 961 32 328 32 1472 2 30 Cuts at: 5980 5982 7325 7357 7960 7992 8291 8323 8624 Size: 2 1343 32 603 32 299 32 301 Cuts at: 8624 8656 9130 9162 9569 9601 11086 11118 11160 Size: 32 474 32 407 32 1485 32 42 Cuts at: 11160 11192 1373 Size: 32 2626 Fragments arranged by size: 2626 1485 1472 1343 961 888 603 474 407 328 301 301 299 266 135 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 30 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 2868 4798 6651 6939 8775 9161 Size: 988 281 930 1930 1853 288 1836 386 Cuts at: 9161 9789 10035 669 Size: 628 246 3079 Fragments arranged by size: 3079 1930 1853 1836 988 930 628 386 288 281 246 HindIII A'AGCT_T Cuts at: 748 2785 3359 4140 4747 7507 8492 9029 748 Size: 2037 574 781 607 2760 985 537 4164 Fragments arranged by size: 4164 2760 2037 985 781 607 574 537 HpaI GTT'AAC Cuts at: 2868 4798 8775 10035 2868 Size: 1930 3977 1260 5278 Fragments arranged by size: 5278 3977 1930 1260 KpnI G_GTAC'C Cuts at: 9770 9770 Size: 12445 MfeI C'AATT_G Cuts at: 3128 5132 10044 3128 Size: 2004 4912 5529 Fragments arranged by size: 5529 4912 2004 MluI A'CGCG_T Cuts at: 12321 12321 Size: 12445 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3936 5119 11559 11743 1305 Size: 2631 1183 6440 184 2007 Fragments arranged by size: 6440 2631 2007 1183 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 10623 Fragments arranged by size: 10623 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2169 7212 10403 10762 10921 518 Size: 648 1003 5043 3191 359 159 2042 Fragments arranged by size: 5043 3191 2042 1003 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2050 4603 6031 6833 9243 9861 10538 11686 Size: 444 2553 1428 802 2410 618 677 1148 Cuts at: 11686 11931 1606 Size: 245 2120 Fragments arranged by size: 2553 2410 2120 1428 1148 802 677 618 444 245 NcoI C'CATG_G Cuts at: 513 1065 2004 8130 513 Size: 552 939 6126 4828 Fragments arranged by size: 6126 4828 939 552 NdeI CA'TA_TG Cuts at: 387 4407 4917 387 Size: 4020 510 7915 Fragments arranged by size: 7915 4020 510 NheI G'CTAG_C Cuts at: 1052 1052 Size: 12445 NotI GC'GGCC_GC Cuts at: 2045 9892 2045 Size: 7847 4598 NsiI A_TGCA'T Cuts at: 7116 7275 8838 7116 Size: 159 1563 10723 Fragments arranged by size: 10723 1563 159 NspI r_CATG'y Cuts at: 1132 1229 7114 7273 9803 11348 1132 Size: 97 5885 159 2530 1545 2229 Fragments arranged by size: 5885 2530 2229 1545 159 97 PasI CC'CwG_GG Cuts at: 1348 2325 1348 Size: 977 11468 PciI A'CATG_T Cuts at: 11344 11344 Size: 12445 PflMI CCAn_nnn'nTGG Cuts at: 1108 3142 8344 8622 1108 Size: 2034 5202 278 4931 Fragments arranged by size: 5202 4931 2034 278 PfoI T'CCnGG_A Cuts at: 4904 6066 4904 Size: 1162 11283 PmeI GTTT'AAAC Cuts at: 9749 9749 Size: 12445 PmlI CAC'GTG Cuts at: 4568 4568 Size: 12445 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 10514 10546 12027 12059 10514 Size: 32 1481 32 10900 Fragments arranged by size: 10900 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 5280 8632 9695 5280 Size: 3352 1063 8030 Fragments arranged by size: 8030 3352 1063 PshAI GACnn'nnGTC Cuts at: 1613 6833 1613 Size: 5220 7225 PsiI TTA'TAA Cuts at: 4365 8855 9128 10015 4365 Size: 4490 273 887 6795 Fragments arranged by size: 6795 4490 887 273 PspXI vC'TCGA_Gb Cuts at: 6644 6644 Size: 12445 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 5784 5816 9516 9548 5784 Size: 32 3700 32 8681 Fragments arranged by size: 8681 3700 32 32 PstI C_TGCA'G Cuts at: 830 2749 4683 5023 9797 830 Size: 1919 1934 340 4774 3478 Fragments arranged by size: 4774 3478 1934 1919 340 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 12445 PvuII CAG'CTG Cuts at: 4603 6031 6833 4603 Size: 1428 802 10215 Fragments arranged by size: 10215 1428 802 SacI G_AGCT'C Cuts at: 4239 4239 Size: 12445 SalI G'TCGA_C Cuts at: 1936 9787 1936 Size: 7851 4594 SapI GCTCTTCn'nnn_ Cuts at: 4227 4227 Size: 12445 SbfI CC_TGCA'GG Cuts at: 9797 9797 Size: 12445 ScaI AGT'ACT Cuts at: 2977 8424 8964 10693 12405 2977 Size: 5447 540 1729 1712 3017 Fragments arranged by size: 5447 3017 1729 1712 540 SfcI C'TryA_G Cuts at: 826 1045 2745 3710 3825 4644 4679 5019 5613 Size: 219 1700 965 115 819 35 340 594 Cuts at: 5613 6246 6269 9105 9793 10928 11609 11800 826 Size: 633 23 2836 688 1135 681 191 1471 Fragments arranged by size: 2836 1700 1471 1135 965 819 688 681 633 594 340 219 191 115 35 23 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 12445 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 12445 SmaI CCC'GGG Cuts at: 1872 9772 1872 Size: 7900 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2759 4439 4713 4815 4838 5474 Size: 197 926 816 1680 274 102 23 636 Cuts at: 5474 6644 6798 6821 7043 7403 7652 8873 9631 Size: 1170 154 23 222 360 249 1221 758 Cuts at: 9631 10549 11450 11712 820 Size: 918 901 262 1553 Fragments arranged by size: 1680 1553 1221 1170 926 918 901 816 758 636 360 274 262 249 222 197 154 102 23 23 SnaBI TAC'GTA Cuts at: 493 10236 493 Size: 9743 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 12445 SphI G_CATG'C Cuts at: 1132 9803 1132 Size: 8671 3774 SspI AAT'ATT Cuts at: 5 52 5158 10369 5 Size: 47 5106 5211 2081 Fragments arranged by size: 5211 5106 2081 47 StuI AGG'CCT Cuts at: 1517 5619 9642 1517 Size: 4102 4023 4320 Fragments arranged by size: 4320 4102 4023 StyI C'CwwG_G Cuts at: 513 1065 2004 2889 3277 5880 5973 6606 8130 Size: 552 939 885 388 2603 93 633 1524 Cuts at: 8130 8697 9698 9884 513 Size: 567 1001 186 3074 Fragments arranged by size: 3074 2603 1524 1001 939 885 633 567 552 388 186 93 SwaI ATTT'AAAT Cuts at: 7542 7542 Size: 12445 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4502 4541 7863 10472 10489 10642 10827 Size: 1255 2922 39 3322 2609 17 153 185 Cuts at: 10827 11272 325 Size: 445 1498 Fragments arranged by size: 3322 2922 2609 1498 1255 445 185 153 39 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2975 3169 5365 Size: 274 80 33 51 1443 998 194 2196 Cuts at: 5365 6721 8422 8962 9250 10691 12403 96 Size: 1356 1701 540 288 1441 1712 138 Fragments arranged by size: 2196 1712 1701 1443 1441 1356 998 540 288 274 194 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2862 2894 2914 2946 6842 6874 12405 12437 2862 Size: 32 20 32 3896 32 5531 32 2870 Fragments arranged by size: 5531 3896 2870 32 32 32 32 20 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 12445 XbaI T'CTAG_A Cuts at: 3622 7934 9781 3622 Size: 4312 1847 6286 Fragments arranged by size: 6286 4312 1847 XhoI C'TCGA_G Cuts at: 1943 6644 1943 Size: 4701 7744 XmaI C'CCGG_G Cuts at: 1870 9770 1870 Size: 7900 4545 XmnI GAAnn'nnTTC Cuts at: 4281 5937 6288 6584 7318 7392 8543 9484 10574 Size: 1656 351 296 734 74 1151 941 1090 Cuts at: 10574 4281 Size: 6152 Fragments arranged by size: 6152 1656 1151 1090 941 734 351 296 74 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 10253 276 Size: 53 83 186 936 8719 2468 Fragments arranged by size: 8719 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BsiWI BstZ17I EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI RsrII SacII SanDI SexAI SfiI SfoI SrfI XcmI