Restriction map for
pFN21AB7754
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# NO CUT ENZYMES ( 111 enzymes) #
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Acc65I AccI AccII AclI AfaI
AflII AgeI Alw44I AocI ApaI
ApaLI AscI AseI AsnI Asp718I
AspI AviII AvrII BamHI BanI
BbeI BcgI BciVI BclI BfrI
BglI BlnI BlpI Bpu1102I BsaBI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstUI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DrdI EagI EclXI Eco52I
Eco81I EcoNI EcoT22I FbaI FseI
FspI HincII HindII HpaI KasI
KpnI KspI MamI MfeI MluI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 1243 GACGTC GACGT^C
AccIII 1387 TCCGGA T^CCGGA
AcsI 990 GAATTC [GA]^AATT[CT]
AhdI 631 GACCCAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1172 AGCGCT AGC^GCT
ApoI 990 GAATTC [GA]^AATT[CT]
AspEI 631 GACCCAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 972 CCCGGG C^[CT]CG[GA]G
BalI 1038 TGGCCA TGG^CCA
BbsI 368 GAAGAC GAAGAC(2/6)
BglII 741 AGATCT A^GATCT
BpuAI 368 GAAGAC GAAGAC(2/6)
BsaI 645 GAGACC GGTCTC(1/5)
BsaWI 1387 TCCGGA [AT]^CCGG[AT]
BseAI 1387 TCCGGA T^CCGGA
BsmBI 866 GAGACG CGTCTC(1/5)
BsoBI 972 CCCGGG C^[CT]CG[GA]G
BspEI 1387 TCCGGA T^CCGGA
BspMI 350 ACCTGC ACCTGC(4/8)
BstAPI 549 GCAGATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1318 TTCGAA TT^CGAA
DraIII 106 CACGACGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1038 TGGCCA [CT]^GGCC[GA]
Eam1105I 631 GACCCAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1172 AGCGCT AGC^GCT
EcoRI 990 GAATTC G^AATTC
EcoRV 430 GATATC GAT^ATC
FauI 854 GCGGG CCCGC(4/6)
HaeII 1172 AGCGCT [GA]GCGC^[CT]
HindIII 839 AAGCTT A^AGCTT
MluNI 1038 TGGCCA TGG^CCA
MroI 1387 TCCGGA T^CCGGA
MscI 1038 TGGCCA TGG^CCA
MseI 1447 TTAA T^TAA
NspV 1318 TTCGAA TT^CGAA
PflMI 374 CCAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 547 CTGCAG CTGCA^G
PvuII 150 CAGCTG CAG^CTG
SacI 295 GAGCTC GAGCT^C
SapI 928 GAAGAGC GCTCTTC(1/4)
SfuI 1318 TTCGAA TT^CGAA
SmaI 972 CCCGGG CCC^GGG
SmlI 935 CTCAAG C^T[CT][GA]AG
Tru9I 1447 TTAA T^TAA
Van91I 374 CCAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 650 CCAAGCAGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 972 CCCGGG C^CCGGG
XmaI 972 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AciI 1060 CCGC CCGC(-3/-1)
854 GCGG
AcyI 1243 GACGTC G[GA]^CG[CT]C
1451 GACGCC
AspHI 295 GAGCTC G[AT]GC[AT]^C
1369 GAGCAC
BanII 295 GAGCTC G[GA]GC[CT]^C
1017 GAGCCC
BbrPI 130 CACGTG CAC^GTG
1236 CACGTG
BbvCI 718 CCTCAGC CCTCAGC(-5/-2)
216 GCTGAGG
BfaI 31 CTAG C^TAG
1111 CTAG
BsaHI 1243 GACGTC G[GA]^CG[CT]C
1451 GACGCC
BsgI 325 GTGCAG GTGCAG(16/14)
418 GTGCAG
BsiHKAI 295 GAGCTC G[AT]GC[AT]^C
1369 GAGCAC
BspHI 275 TCATGA T^CATGA
311 TCATGA
BstEII 390 GGTCACC G^GT[ACGT]ACC
628 GGTGACC
BstPI 390 GGTCACC G^GT[ACGT]ACC
628 GGTGACC
BstXI 737 CCACAGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
947 CCACACCTGTGG
Cac8I 820 GCCTGC GC[ACGT]^[ACGT]GC
1015 GCGAGC
CfoI 1173 GCGC GCG^C
1377 GCGC
DsaI 67 CCACGG C^C[GA][CT]GG
399 CCACGG
EcoO65I 390 GGTCACC G^GT[ACGT]ACC
628 GGTGACC
EcoT14I 659 CCTTGG C^C[AT][AT]GG
1049 CCAAGG
HhaI 1173 GCGC GCG^C
1377 GCGC
Hin1I 1243 GACGTC G[GA]^CG[CT]C
1451 GACGCC
HinP1I 1173 GCGC G^CGC
1377 GCGC
MaeI 31 CTAG C^TAG
1111 CTAG
MspA1I 150 CAGCTG C[AC]G^C[GT]G
852 CAGCGG
NspI 254 ACATGC [GA]CATG^[CT]
1069 GCATGT
PmaCI 130 CACGTG CAC^GTG
1236 CACGTG
PmlI 130 CACGTG CAC^GTG
1236 CACGTG
RcaI 275 TCATGA T^CATGA
311 TCATGA
SfcI 547 CTGCAG C^T[GA][CT]AG
1350 CTACAG
StyI 659 CCTTGG C^C[AT][AT]GG
1049 CCAAGG
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AflIII AluI AlwI AlwNI Asp700I
AvaII BbvI BcnI BmyI BpmI
BsaAI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI Sau3AI Sau96I ScrFI SfaNI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI