(Circular) (Six-base) MAPSORT of: pFN21AB7754.seq Check: 3317 from: 1 to: 6193 pFN21AB7754 6193 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3252 4003 278 Size: 53 83 186 936 1716 751 2468 Fragments arranged by size: 2468 1716 936 751 186 83 53 AccI GT'mk_AC Cuts at: 1937 3536 1937 Size: 1599 4594 Acc65I G'GTAC_C Cuts at: 3514 3514 Size: 6193 AclI AA'CG_TT Cuts at: 1563 4320 4693 1563 Size: 2757 373 3063 Fragments arranged by size: 3063 2757 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2128 2469 2699 3389 3418 4256 5640 5908 Size: 496 341 230 690 29 838 1384 268 Cuts at: 5908 1632 Size: 1917 Fragments arranged by size: 1917 1384 838 690 496 341 268 230 29 AfeI AGC'GCT Cuts at: 3179 3179 Size: 6193 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5996 AflIII A'CryG_T Cuts at: 1203 2484 3081 3101 3240 3242 5092 6069 1203 Size: 1281 597 20 139 2 1850 977 1327 Fragments arranged by size: 1850 1327 1281 977 597 139 20 2 AgeI A'CCGG_T Cuts at: 5024 5024 Size: 6193 AhdI GACnn_n'nnGTC Cuts at: 2641 4922 2641 Size: 2281 3912 AleI CACnn'nnGTG Cuts at: 1166 2957 1166 Size: 1791 4402 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6193 AlwNI CAG_nnn'CTG Cuts at: 2188 2254 2560 3017 3936 5508 2188 Size: 66 306 457 919 1572 2873 Fragments arranged by size: 2873 1572 919 457 306 66 ApaLI G'TGCA_C Cuts at: 4250 5406 4250 Size: 1156 5037 ApoI r'AATT_y Cuts at: 1493 2995 3502 3728 3880 5989 1493 Size: 1502 507 226 152 2109 1697 Fragments arranged by size: 2109 1697 1502 507 226 152 AseI AT'TA_AT Cuts at: 160 1032 4747 160 Size: 872 3715 1606 Fragments arranged by size: 3715 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6193 AvaI C'yCGr_G Cuts at: 1870 1943 2977 3518 1870 Size: 73 1034 541 4545 Fragments arranged by size: 4545 1034 541 73 BamHI G'GATC_C Cuts at: 3523 3890 3523 Size: 367 5826 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3514 4969 618 Size: 325 217 627 1727 1455 1842 Fragments arranged by size: 1842 1727 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2304 3026 3512 1391 Size: 913 722 486 4072 Fragments arranged by size: 4072 913 722 486 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2380 928 Size: 971 481 4741 Fragments arranged by size: 4741 971 481 BbvCI CC'TCA_GC Cuts at: 2222 2724 2222 Size: 502 5691 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4384 4418 1903 Size: 34 2447 34 3678 Fragments arranged by size: 3678 2447 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4085 5295 4085 Size: 1210 4983 BclI T'GATC_A Cuts at: 1548 1548 Size: 6193 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4804 136 Size: 107 122 71 631 605 3132 1525 Fragments arranged by size: 3132 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2746 6188 2746 Size: 3442 2751 BlpI GC'TnA_GC Cuts at: 3610 3610 Size: 6193 Bme1580I G_kGCm'C Cuts at: 1790 4254 5410 1790 Size: 2464 1156 2573 Fragments arranged by size: 2573 2464 1156 BmgBI CAC'GTC Cuts at: 1318 2116 2485 3082 1318 Size: 798 369 597 4429 Fragments arranged by size: 4429 798 597 369 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3020 3924 4882 Size: 416 53 83 399 24 1603 904 958 Cuts at: 4882 442 Size: 1753 Fragments arranged by size: 1753 1603 958 904 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6193 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2288 2369 2443 4853 1239 Size: 1049 81 74 2410 2579 Fragments arranged by size: 2579 2410 1049 81 74 Bpu10I CC'TnA_GC Cuts at: 2222 2724 2222 Size: 502 5691 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2925 4318 5183 5481 2925 Size: 1393 865 298 3637 Fragments arranged by size: 3637 1393 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2644 4856 882 Size: 617 1145 2212 2219 Fragments arranged by size: 2219 2212 1145 617 BsaAI yAC'GTr Cuts at: 493 1204 2137 3102 3243 3984 493 Size: 711 933 965 141 741 2702 Fragments arranged by size: 2702 965 933 741 711 141 BsaBI GATnn'nnATC Cuts at: 6187 6187 Size: 6193 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3249 3457 4000 4382 Size: 53 83 186 936 1716 208 543 382 Cuts at: 4382 5017 275 Size: 635 1451 Fragments arranged by size: 1716 1451 936 635 543 382 208 186 83 53 BsaWI w'CCGG_w Cuts at: 3392 4626 5024 5298 5445 5856 3392 Size: 1234 398 274 147 411 3729 Fragments arranged by size: 3729 1234 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5968 Fragments arranged by size: 5968 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2494 2883 2908 1186 Size: 1308 389 25 4471 Fragments arranged by size: 4471 1308 389 25 BseYI C'CCAG_C Cuts at: 2987 5396 5892 2987 Size: 2409 496 3288 Fragments arranged by size: 3288 2409 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2351 2444 1163 Size: 573 615 93 4912 Fragments arranged by size: 4912 615 573 93 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3643 4404 5432 1540 Size: 275 186 1642 761 1028 2301 Fragments arranged by size: 2301 1642 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2304 3378 4254 4339 5410 2304 Size: 1074 876 85 1071 3087 Fragments arranged by size: 3087 1074 1071 876 85 BsmI GAATG_Cn' Cuts at: 3704 3797 3704 Size: 93 6100 BsmBI CGTCTCn'nnnn_ Cuts at: 2865 2865 Size: 6193 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2304 3026 3378 3512 4254 4339 5410 Size: 399 514 722 352 134 742 85 1071 Cuts at: 5410 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 722 514 399 352 134 85 BspEI T'CCGG_A Cuts at: 3392 5856 3392 Size: 2464 3729 BspHI T'CATG_A Cuts at: 2280 2316 4082 5812 2280 Size: 36 1766 1730 2661 Fragments arranged by size: 2661 1766 1730 36 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2364 3548 844 Size: 1520 1184 3489 Fragments arranged by size: 3489 1520 1184 BsrBI CCG'CTC Cuts at: 1394 1823 4080 1394 Size: 429 2257 3507 Fragments arranged by size: 3507 2257 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4688 4862 65 Size: 1183 3440 174 1396 Fragments arranged by size: 3440 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4837 5024 1700 Size: 110 3027 187 2869 Fragments arranged by size: 3027 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6193 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6193 BssSI C'ACGA_G Cuts at: 4253 5265 4253 Size: 1012 5181 BstAPI GCAn_nnn'nTGC Cuts at: 794 2560 794 Size: 1766 4427 BstBI TT'CG_AA Cuts at: 3324 4997 3324 Size: 1673 4520 BstEII G'GTnAC_C Cuts at: 1184 2395 2633 1184 Size: 1211 238 4744 Fragments arranged by size: 4744 1211 238 BstXI CCAn_nnnn'nTGG Cuts at: 2749 2959 2749 Size: 210 5983 BstYI r'GATC_y Cuts at: 1972 2250 2556 2674 2746 3523 3890 4275 4292 Size: 278 306 118 72 777 367 385 17 Cuts at: 4292 5744 5830 5842 6188 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 777 385 367 346 306 278 118 86 72 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2072 2404 513 Size: 552 939 68 332 4302 Fragments arranged by size: 4302 939 552 332 68 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5454 Fragments arranged by size: 5454 683 56 BtsI GCAGTG_nn' Cuts at: 3059 3709 4503 4523 3059 Size: 650 794 20 4729 Fragments arranged by size: 4729 794 650 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6193 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6158 DraI TTT'AAA Cuts at: 3497 3497 Size: 6193 DraIII CAC_nnn'GTG Cuts at: 2116 2116 Size: 6193 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5200 809 Size: 507 3884 1802 Fragments arranged by size: 3884 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3043 3640 4529 Size: 54 1425 50 275 18 1213 597 889 Cuts at: 4529 6158 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1213 889 597 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3640 1537 Size: 275 1828 4090 Fragments arranged by size: 4090 1828 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2688 2928 3086 4123 1353 Size: 1335 240 158 1037 3423 Fragments arranged by size: 3423 1335 1037 240 158 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4780 5154 5300 387 Size: 1064 138 3191 374 146 1280 Fragments arranged by size: 3191 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2302 2302 Size: 6193 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2128 2288 2369 2443 2469 2699 3389 Size: 393 496 160 81 74 26 230 690 Cuts at: 3389 3418 4256 4853 5640 5908 1239 Size: 29 838 597 787 268 1524 Fragments arranged by size: 1524 838 787 690 597 496 393 268 230 160 81 74 29 26 EcoO109I rG'GnC_Cy Cuts at: 2353 2569 2773 3014 3234 2353 Size: 216 204 241 220 5312 Fragments arranged by size: 5312 241 220 216 204 EcoRI G'AATT_C Cuts at: 2995 3502 3880 2995 Size: 507 378 5308 Fragments arranged by size: 5308 507 378 EcoRV GAT'ATC Cuts at: 2437 2437 Size: 6193 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2680 3298 2680 Size: 618 5575 FspI TGC'GCA Cuts at: 4699 4699 Size: 6193 HaeII r_GCGC'y Cuts at: 1678 3181 5340 1678 Size: 1503 2159 2531 Fragments arranged by size: 2531 2159 1503 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2627 2659 2903 2935 4834 Size: 32 135 32 1055 32 244 32 1899 Cuts at: 4834 4866 4908 4940 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1899 1055 244 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3537 3783 669 Size: 988 281 1599 246 3079 Fragments arranged by size: 3079 1599 988 281 246 HindIII A'AGCT_T Cuts at: 748 2844 748 Size: 2096 4097 HpaI GTT'AAC Cuts at: 3783 3783 Size: 6193 KpnI G_GTAC'C Cuts at: 3518 3518 Size: 6193 MfeI C'AATT_G Cuts at: 3792 3792 Size: 6193 MluI A'CGCG_T Cuts at: 6069 6069 Size: 6193 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2043 2151 5307 5491 1305 Size: 738 108 3156 184 2007 Fragments arranged by size: 3156 2007 738 184 108 MscI TGG'CCA Cuts at: 10 64 1489 1832 3045 10 Size: 54 1425 343 1213 3158 Fragments arranged by size: 3158 1425 1213 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2026 2264 2957 3039 4151 4510 4669 Size: 648 860 238 693 82 1112 359 159 Cuts at: 4669 518 Size: 2042 Fragments arranged by size: 2042 1112 860 693 648 359 238 159 82 MspA1I CmG'CkG Cuts at: 1606 2157 2859 3609 4286 5434 5679 1606 Size: 551 702 750 677 1148 245 2120 Fragments arranged by size: 2120 1148 750 702 677 551 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4702 Fragments arranged by size: 4702 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6193 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6193 NotI GC'GGCC_GC Cuts at: 3640 3640 Size: 6193 NspI r_CATG'y Cuts at: 1132 1229 2263 3078 3551 5096 1132 Size: 97 1034 815 473 1545 2229 Fragments arranged by size: 2229 1545 1034 815 473 97 PasI CC'CwG_GG Cuts at: 1348 2565 1348 Size: 1217 4976 PciI A'CATG_T Cuts at: 5092 5092 Size: 6193 PflMI CCAn_nnn'nTGG Cuts at: 1108 2385 1108 Size: 1277 4916 PfoI T'CCnGG_A Cuts at: 2253 2582 2253 Size: 329 5864 PmeI GTTT'AAAC Cuts at: 3497 3497 Size: 6193 PmlI CAC'GTG Cuts at: 2137 3243 2137 Size: 1106 5087 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4262 4294 5775 5807 4262 Size: 32 1481 32 4648 Fragments arranged by size: 4648 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2353 2569 2773 2353 Size: 216 204 5773 Fragments arranged by size: 5773 216 204 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6193 PsiI TTA'TAA Cuts at: 3763 3763 Size: 6193 PstI C_TGCA'G Cuts at: 830 2556 3545 830 Size: 1726 989 3478 Fragments arranged by size: 3478 1726 989 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6193 PvuII CAG'CTG Cuts at: 2157 2157 Size: 6193 SacI G_AGCT'C Cuts at: 2304 2304 Size: 6193 SalI G'TCGA_C Cuts at: 1936 3535 1936 Size: 1599 4594 SanDI GG'GwC_CC Cuts at: 2569 2569 Size: 6193 SapI GCTCTTCn'nnn_ Cuts at: 2928 2928 Size: 6193 SbfI CC_TGCA'GG Cuts at: 3545 3545 Size: 6193 ScaI AGT'ACT Cuts at: 4441 6153 4441 Size: 1712 4481 SfcI C'TryA_G Cuts at: 826 1045 2552 3355 3541 4676 5357 5548 826 Size: 219 1507 803 186 1135 681 191 1471 Fragments arranged by size: 1507 1471 1135 803 681 219 191 186 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6193 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6193 SmaI CCC'GGG Cuts at: 1872 2979 3520 1872 Size: 1107 541 4545 Fragments arranged by size: 4545 1107 541 SmlI C'TyrA_G Cuts at: 820 1017 1943 2940 4297 5198 5460 820 Size: 197 926 997 1357 901 262 1553 Fragments arranged by size: 1553 1357 997 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3984 493 Size: 3491 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6193 SphI G_CATG'C Cuts at: 1132 3551 1132 Size: 2419 3774 SspI AAT'ATT Cuts at: 5 52 4117 5 Size: 47 4065 2081 Fragments arranged by size: 4065 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6193 StyI C'CwwG_G Cuts at: 513 1065 2004 2664 3054 3632 513 Size: 552 939 660 390 578 3074 Fragments arranged by size: 3074 939 660 578 552 390 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3284 4220 4237 4390 4575 5020 325 Size: 1255 1704 936 17 153 185 445 1498 Fragments arranged by size: 1704 1498 1255 936 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4439 6151 96 Size: 274 80 33 51 1443 2462 1712 138 Fragments arranged by size: 2462 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6153 6185 6153 Size: 32 6161 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6193 XbaI T'CTAG_A Cuts at: 3529 3529 Size: 6193 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2662 2662 Size: 6193 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6193 XmaI C'CCGG_G Cuts at: 1870 2977 3518 1870 Size: 1107 541 4545 Fragments arranged by size: 4545 1107 541 XmnI GAAnn'nnTTC Cuts at: 3179 3263 3320 3347 4322 3179 Size: 84 57 27 975 5050 Fragments arranged by size: 5050 975 84 57 27 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3250 4001 276 Size: 53 83 186 936 1716 751 2468 Fragments arranged by size: 2468 1716 936 751 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI