(Circular) (Six-base) MAPSORT of: pFN21AB8336.seq Check: 4951 from: 1 to: 6178 pFN21AB8336 6178 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3988 278 Size: 53 83 186 936 2452 2468 Fragments arranged by size: 2468 2452 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3521 1937 Size: 1584 4594 Acc65I G'GTAC_C Cuts at: 3499 3499 Size: 6178 AclI AA'CG_TT Cuts at: 1563 4305 4678 1563 Size: 2742 373 3063 Fragments arranged by size: 3063 2742 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2610 2718 2902 2986 3070 3154 3238 3406 Size: 978 108 184 84 84 84 84 168 Cuts at: 3406 4241 5625 5893 1632 Size: 835 1384 268 1917 Fragments arranged by size: 1917 1384 978 835 268 184 168 108 84 84 84 84 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5981 AflIII A'CryG_T Cuts at: 1203 3153 3237 5077 6054 1203 Size: 1950 84 1840 977 1327 Fragments arranged by size: 1950 1840 1327 977 84 AgeI A'CCGG_T Cuts at: 5009 5009 Size: 6178 AhdI GACnn_n'nnGTC Cuts at: 4907 4907 Size: 6178 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6178 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6178 AlwNI CAG_nnn'CTG Cuts at: 2236 2269 2296 2395 3261 3408 3921 5493 2236 Size: 33 27 99 866 147 513 1572 2921 Fragments arranged by size: 2921 1572 866 513 147 99 33 27 ApaLI G'TGCA_C Cuts at: 3122 4235 5391 3122 Size: 1113 1156 3909 Fragments arranged by size: 3909 1156 1113 ApoI r'AATT_y Cuts at: 1493 2744 3487 3713 3865 5974 1493 Size: 1251 743 226 152 2109 1697 Fragments arranged by size: 2109 1697 1251 743 226 152 AseI AT'TA_AT Cuts at: 160 1032 4732 160 Size: 872 3700 1606 Fragments arranged by size: 3700 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6178 AvaI C'yCGr_G Cuts at: 1870 1943 2243 2680 2852 3503 1870 Size: 73 300 437 172 651 4545 Fragments arranged by size: 4545 651 437 300 172 73 BamHI G'GATC_C Cuts at: 2413 3372 3456 3508 3875 2413 Size: 959 84 52 367 4716 Fragments arranged by size: 4716 959 367 84 52 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2675 3499 4954 618 Size: 325 217 627 888 824 1455 1842 Fragments arranged by size: 1842 1455 888 824 627 325 217 BanII G_rGCy'C Cuts at: 1391 2033 2338 2430 3350 3497 1391 Size: 642 305 92 920 147 4072 Fragments arranged by size: 4072 920 642 305 147 92 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2745 2873 2888 928 Size: 971 846 128 15 4218 Fragments arranged by size: 4218 971 846 128 15 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2205 2239 2917 2951 4369 4403 1903 Size: 34 268 34 678 34 1418 34 3678 Fragments arranged by size: 3678 1418 678 268 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4070 5280 4070 Size: 1210 4968 BclI T'GATC_A Cuts at: 1548 1548 Size: 6178 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4789 136 Size: 107 122 71 631 605 3117 1525 Fragments arranged by size: 3117 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2580 6173 2580 Size: 3593 2585 BlpI GC'TnA_GC Cuts at: 3265 3433 3595 3265 Size: 168 162 5848 Fragments arranged by size: 5848 168 162 Bme1580I G_kGCm'C Cuts at: 1790 3126 4239 5395 1790 Size: 1336 1113 1156 2573 Fragments arranged by size: 2573 1336 1156 1113 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6178 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2027 3909 4867 Size: 416 53 83 399 24 610 1882 958 Cuts at: 4867 442 Size: 1753 Fragments arranged by size: 1882 1753 958 610 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6178 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2269 2569 2269 Size: 300 5878 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2291 2306 3066 3318 4838 1239 Size: 1052 15 760 252 1520 2579 Fragments arranged by size: 2579 1520 1052 760 252 15 Bpu10I CC'TnA_GC Cuts at: 2027 2027 Size: 6178 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4303 5168 5466 4303 Size: 865 298 5015 Fragments arranged by size: 5015 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2483 4841 882 Size: 617 984 2358 2219 Fragments arranged by size: 2358 2219 984 617 BsaAI yAC'GTr Cuts at: 493 1204 3969 493 Size: 711 2765 2702 Fragments arranged by size: 2765 2702 711 BsaBI GATnn'nnATC Cuts at: 3461 6172 3461 Size: 2711 3467 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3985 4367 5002 275 Size: 53 83 186 936 2452 382 635 1451 Fragments arranged by size: 2452 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4611 5009 5283 5430 5841 4611 Size: 398 274 147 411 4948 Fragments arranged by size: 4948 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2699 2729 2761 2791 1178 Size: 30 165 30 1296 30 32 30 4565 Fragments arranged by size: 4565 1296 165 32 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2090 2192 3340 1186 Size: 904 102 1148 4024 Fragments arranged by size: 4024 1148 904 102 BseYI C'CCAG_C Cuts at: 2940 3108 5381 5877 2940 Size: 168 2273 496 3241 Fragments arranged by size: 3241 2273 496 168 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2130 3421 1163 Size: 573 394 1291 3920 Fragments arranged by size: 3920 1291 573 394 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3628 4389 5417 1540 Size: 275 186 1627 761 1028 2301 Fragments arranged by size: 2301 1627 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2930 3126 3350 3366 4239 4324 5395 2930 Size: 196 224 16 873 85 1071 3713 Fragments arranged by size: 3713 1071 873 224 196 85 16 BsmI GAATG_Cn' Cuts at: 3689 3782 3689 Size: 93 6085 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2033 2338 2430 2930 3126 3350 3366 Size: 399 243 305 92 500 196 224 16 Cuts at: 3366 3497 4239 4324 5395 1391 Size: 131 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 500 399 305 243 224 196 131 92 85 16 BspEI T'CCGG_A Cuts at: 5841 5841 Size: 6178 BspHI T'CATG_A Cuts at: 4067 5797 4067 Size: 1730 4448 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3533 844 Size: 2689 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2352 3136 4065 1394 Size: 429 529 784 929 3507 Fragments arranged by size: 3507 929 784 529 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2127 4673 4847 65 Size: 1183 879 2546 174 1396 Fragments arranged by size: 2546 1396 1183 879 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4822 5009 1700 Size: 110 3012 187 2869 Fragments arranged by size: 3012 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6178 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6178 BssSI C'ACGA_G Cuts at: 2195 4238 5250 2195 Size: 2043 1012 3123 Fragments arranged by size: 3123 2043 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6178 BstBI TT'CG_AA Cuts at: 4982 4982 Size: 6178 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6178 BstXI CCAn_nnnn'nTGG Cuts at: 2040 2040 Size: 6178 BstYI r'GATC_y Cuts at: 1972 2316 2380 2413 2580 2607 3372 3456 3508 Size: 344 64 33 167 27 765 84 52 Cuts at: 3508 3875 4260 4277 5729 5815 5827 6173 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 765 385 367 346 344 167 86 84 64 52 33 27 17 12 Bsu36I CC'TnA_GG Cuts at: 2544 2544 Size: 6178 BtgI C'CryG_G Cuts at: 513 1065 2004 2039 513 Size: 552 939 35 4652 Fragments arranged by size: 4652 939 552 35 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5439 Fragments arranged by size: 5439 683 56 BtsI GCAGTG_nn' Cuts at: 3694 4488 4508 3694 Size: 794 20 5364 Fragments arranged by size: 5364 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6178 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6143 DraI TTT'AAA Cuts at: 3482 3482 Size: 6178 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5185 809 Size: 507 3869 1802 Fragments arranged by size: 3869 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2662 2702 Size: 54 1425 50 275 18 177 655 40 Cuts at: 2702 3244 3625 4514 6143 8 Size: 542 381 889 1629 43 Fragments arranged by size: 1629 1425 889 655 542 381 275 177 54 50 43 40 18 EagI C'GGCC_G Cuts at: 1537 1812 3625 1537 Size: 275 1813 4090 Fragments arranged by size: 4090 1813 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2040 2076 2792 4108 1353 Size: 687 36 716 1316 3423 Fragments arranged by size: 3423 1316 716 687 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2121 4765 5139 5285 387 Size: 1064 138 532 2644 374 146 1280 Fragments arranged by size: 2644 1280 1064 532 374 146 138 EcoICRI GAG'CTC Cuts at: 3348 3348 Size: 6178 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2291 2306 2610 2718 2902 2986 3066 Size: 393 659 15 304 108 184 84 80 Cuts at: 3066 3070 3154 3238 3318 3406 4241 4838 5625 Size: 4 84 84 80 88 835 597 787 Cuts at: 5625 5893 1239 Size: 268 1524 Fragments arranged by size: 1524 835 787 659 597 393 304 268 184 108 88 84 84 84 80 80 15 4 EcoNI CCTnn'n_nnAGG Cuts at: 2099 2099 Size: 6178 EcoO109I rG'GnC_Cy Cuts at: 2200 2630 2802 2200 Size: 430 172 5576 Fragments arranged by size: 5576 430 172 EcoRI G'AATT_C Cuts at: 3487 3865 3487 Size: 378 5800 FspI TGC'GCA Cuts at: 4684 4684 Size: 6178 HaeII r_GCGC'y Cuts at: 1678 2852 5325 1678 Size: 1174 2473 2531 Fragments arranged by size: 2531 2473 1174 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2168 2200 2761 2793 2860 Size: 32 135 32 596 32 561 32 67 Cuts at: 2860 2892 3265 3297 4819 4851 4893 4925 1373 Size: 32 373 32 1522 32 42 32 2626 Fragments arranged by size: 2626 1522 596 561 373 135 67 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3522 3768 669 Size: 988 281 1584 246 3079 Fragments arranged by size: 3079 1584 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6178 HpaI GTT'AAC Cuts at: 3768 3768 Size: 6178 KpnI G_GTAC'C Cuts at: 3503 3503 Size: 6178 MfeI C'AATT_G Cuts at: 3777 3777 Size: 6178 MluI A'CGCG_T Cuts at: 3153 3237 6054 3153 Size: 84 2817 3277 Fragments arranged by size: 3277 2817 84 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3301 5292 5476 1305 Size: 1996 1991 184 2007 Fragments arranged by size: 2007 1996 1991 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2704 10 Size: 54 1425 343 177 695 3484 Fragments arranged by size: 3484 1425 695 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 3313 3467 4136 4495 4654 518 Size: 648 2147 154 669 359 159 2042 Fragments arranged by size: 2147 2042 669 648 359 159 154 MspA1I CmG'CkG Cuts at: 1606 2366 3118 3594 4271 5419 5664 1606 Size: 760 752 476 677 1148 245 2120 Fragments arranged by size: 2120 1148 760 752 677 476 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4687 Fragments arranged by size: 4687 939 552 NdeI CA'TA_TG Cuts at: 387 2890 387 Size: 2503 3675 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6178 NotI GC'GGCC_GC Cuts at: 3625 3625 Size: 6178 NruI TCG'CGA Cuts at: 2817 2817 Size: 6178 NspI r_CATG'y Cuts at: 1132 1229 2538 3536 5081 1132 Size: 97 1309 998 1545 2229 Fragments arranged by size: 2229 1545 1309 998 97 PasI CC'CwG_GG Cuts at: 1348 2101 1348 Size: 753 5425 PciI A'CATG_T Cuts at: 5077 5077 Size: 6178 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6178 PfoI T'CCnGG_A Cuts at: 2416 2416 Size: 6178 PmeI GTTT'AAAC Cuts at: 3482 3482 Size: 6178 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2674 2706 4247 4279 5760 5792 2674 Size: 32 1541 32 1481 32 3060 Fragments arranged by size: 3060 1541 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2802 2802 Size: 6178 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6178 PsiI TTA'TAA Cuts at: 3396 3748 3396 Size: 352 5826 PspXI vC'TCGA_Gb Cuts at: 2680 2852 2680 Size: 172 6006 PstI C_TGCA'G Cuts at: 830 2241 2900 3530 830 Size: 1411 659 630 3478 Fragments arranged by size: 3478 1411 659 630 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6178 SacI G_AGCT'C Cuts at: 3350 3350 Size: 6178 SalI G'TCGA_C Cuts at: 1936 3520 1936 Size: 1584 4594 SbfI CC_TGCA'GG Cuts at: 3530 3530 Size: 6178 ScaI AGT'ACT Cuts at: 4426 6138 4426 Size: 1712 4466 SexAI A'CCwGG_T Cuts at: 2946 2946 Size: 6178 SfcI C'TryA_G Cuts at: 826 1045 2237 2896 3526 4661 5342 5533 826 Size: 219 1192 659 630 1135 681 191 1471 Fragments arranged by size: 1471 1192 1135 681 659 630 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6178 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6178 SmaI CCC'GGG Cuts at: 1872 3505 1872 Size: 1633 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2680 2852 4282 5183 5445 820 Size: 197 926 737 172 1430 901 262 1553 Fragments arranged by size: 1553 1430 926 901 737 262 197 172 SnaBI TAC'GTA Cuts at: 493 3969 493 Size: 3476 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6178 SphI G_CATG'C Cuts at: 1132 3536 1132 Size: 2404 3774 SspI AAT'ATT Cuts at: 5 52 4102 5 Size: 47 4050 2081 Fragments arranged by size: 4050 2081 47 StuI AGG'CCT Cuts at: 1517 2999 3335 3419 1517 Size: 1482 336 84 4276 Fragments arranged by size: 4276 1482 336 84 StyI C'CwwG_G Cuts at: 513 1065 2004 2256 2512 3617 513 Size: 552 939 252 256 1105 3074 Fragments arranged by size: 3074 1105 939 552 256 252 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2288 2317 2615 3029 3122 4205 4222 Size: 1255 708 29 298 414 93 1083 17 Cuts at: 4222 4375 4560 5005 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1083 708 445 414 298 185 153 93 29 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4424 6136 96 Size: 274 80 33 51 1443 2447 1712 138 Fragments arranged by size: 2447 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6138 6170 6138 Size: 32 6146 Tth111I GACn'n_nGTC Cuts at: 1316 2590 1316 Size: 1274 4904 XbaI T'CTAG_A Cuts at: 3514 3514 Size: 6178 XhoI C'TCGA_G Cuts at: 1943 2680 2852 1943 Size: 737 172 5269 Fragments arranged by size: 5269 737 172 XmaI C'CCGG_G Cuts at: 1870 3503 1870 Size: 1633 4545 XmnI GAAnn'nnTTC Cuts at: 2831 2915 2999 3167 3419 4307 2831 Size: 84 84 168 252 888 4702 Fragments arranged by size: 4702 888 252 168 84 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3986 276 Size: 53 83 186 936 2452 2468 Fragments arranged by size: 2468 2452 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PmlI PspOMI PsrI PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI