Restriction map for
pFN21AB7685
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# NO CUT ENZYMES ( 161 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI ApaI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRV
EcoT22I FauI FbaI Fnu4HI FseI
FspI HapII HgaI Hin1I HincII
HindII HindIII HpaI HpaII ItaI
KasI KpnI KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MspI MunI MvnI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI TseI
Tth111I TthHB8I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AciI 1028 CCGC CCGC(-3/-1)
Alw44I 305 GTGCAC G^TGCAC
AlwI 351 GGATC GGATC(4/5)
AocI 19 CCTGAGG CC^T[ACGT]AGG
Aor51HI 824 AGCGCT AGC^GCT
ApaLI 305 GTGCAC G^TGCAC
BanII 190 GAGCTC G[GA]GC[CT]^C
BbsI 698 GAAGAC GAAGAC(2/6)
BpuAI 698 GAAGAC GAAGAC(2/6)
BsgI 93 GTGCAG GTGCAG(16/14)
BsmAI 319 GAGAC GTCTC(1/5)
BstXI 5 CCACAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 351 GGATCT [GA]^GATC[CT]
Bsu36I 19 CCTGAGG CC^T[ACGT]AGG
DraII 386 AGGTCCC [GA]G^G[ACGT]CC[CT]
EarI 550 GAAGAG CTCTTC(1/4)
Eco47III 824 AGCGCT AGC^GCT
Eco81I 19 CCTGAGG CC^T[ACGT]AGG
EcoNI 437 CCTTGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 386 AGGTCCC [GA]G^G[ACGT]CC[CT]
HaeII 824 AGCGCT [GA]GCGC^[CT]
Ksp632I 550 GAAGAG CTCTTC(1/4)
MaeIII 337 GTGAC ^GT[ACGT]AC
MflI 351 GGATCT [GA]^GATC[CT]
MseI 47 TTAA T^TAA
MwoI 183 GCTGTGGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 449 CATATG CA^TATG
PflMI 316 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 386 AGGTCCC [GA]G^G[AT]CC[CT]
SacI 190 GAGCTC GAGCT^C
SfcI 117 CTATAG C^T[GA][CT]AG
StuI 979 AGGCCT AGG^CCT
Tru9I 47 TTAA T^TAA
Tsp45I 337 GTGAC ^GT[GC]AC
Van91I 316 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 312 CCATCCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 351 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 589 GAATTC [GA]^AATT[CT]
1019 GAATTC
AfaI 343 GTAC GT^AC
853 GTAC
ApoI 589 GAATTC [GA]^AATT[CT]
1019 GAATTC
Asp700I 726 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
894 GAAAGACTTC
AspHI 190 GAGCTC G[AT]GC[AT]^C
305 GTGCAC
BbvCI 631 CCTCAGC CCTCAGC(-5/-2)
207 GCTGAGG
BfaI 256 CTAG C^TAG
536 CTAG
BmyI 190 GAGCTC G[AGT]GC[ACT]^C
305 GTGCAC
BseRI 629 CTCCTC GAGGAG(10/8)
328 GAGGAG
BsiHKAI 190 GAGCTC G[AT]GC[AT]^C
305 GTGCAC
BsmI 217 GAATGC GAATGC(1/-1)
272 GCATTC
Bsp1286I 190 GAGCTC G[AGT]GC[ACT]^C
305 GTGCAC
Cac8I 291 GCAAGC GC[ACGT]^[ACGT]GC
303 GCGTGC
CfoI 825 GCGC GCG^C
909 GCGC
DpnII 352 GATC ^GATC
674 GATC
EcoRI 589 GAATTC G^AATTC
1019 GAATTC
HhaI 825 GCGC GCG^C
909 GCGC
HinP1I 825 GCGC G^CGC
909 GCGC
HphI 526 GGTGA GGTGA(8/7)
946 GGTGA
MaeI 256 CTAG C^TAG
536 CTAG
MboI 352 GATC ^GATC
674 GATC
MspA1I 181 CAGCTG C[AC]G^C[GT]G
490 CAGCTG
NdeII 352 GATC ^GATC
674 GATC
NlaIII 296 CATG CATG^
420 CATG
PvuII 181 CAGCTG CAG^CTG
490 CAGCTG
RsaI 343 GTAC GT^AC
853 GTAC
Sau3AI 352 GATC ^GATC
674 GATC
XmnI 726 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
894 GAAAGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AluI AlwNI AvaII BpmI BsaJI
BsiYI BslI BsmFI BspMI BsrI
BssKI BstF5I BstNI Cfr13I DdeI
EcoRII EcoT14I FokI HaeIII HinfI
MboII MnlI MslI MvaI NlaIV
Sau96I ScrFI SfaNI StyI TfiI
Tsp509I TspRI