(Circular) (Six-base) MAPSORT of: pFN21AB7685.seq Check: 3964 from: 1 to: 5749 pFN21AB7685 5749 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3559 278 Size: 53 83 186 936 2023 2468 Fragments arranged by size: 2468 2023 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3092 1937 Size: 1155 4594 Acc65I G'GTAC_C Cuts at: 3070 3070 Size: 5749 AclI AA'CG_TT Cuts at: 1563 3876 4249 1563 Size: 2313 373 3063 Fragments arranged by size: 3063 2313 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2119 2890 3812 5196 5464 1632 Size: 487 771 922 1384 268 1917 Fragments arranged by size: 1917 1384 922 771 487 268 AfeI AGC'GCT Cuts at: 2831 2831 Size: 5749 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5552 AflIII A'CryG_T Cuts at: 1203 4648 5625 1203 Size: 3445 977 1327 Fragments arranged by size: 3445 1327 977 AgeI A'CCGG_T Cuts at: 4580 4580 Size: 5749 AhdI GACnn_n'nnGTC Cuts at: 4478 4478 Size: 5749 AleI CACnn'nnGTG Cuts at: 1166 2095 2949 1166 Size: 929 854 3966 Fragments arranged by size: 3966 929 854 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5749 AlwNI CAG_nnn'CTG Cuts at: 2212 2272 2327 3492 5064 2212 Size: 60 55 1165 1572 2897 Fragments arranged by size: 2897 1572 1165 60 55 ApaLI G'TGCA_C Cuts at: 2310 3806 4962 2310 Size: 1496 1156 3097 Fragments arranged by size: 3097 1496 1156 ApoI r'AATT_y Cuts at: 1493 2594 3024 3058 3284 3436 5545 1493 Size: 1101 430 34 226 152 2109 1697 Fragments arranged by size: 2109 1697 1101 430 226 152 34 AseI AT'TA_AT Cuts at: 160 1032 4303 160 Size: 872 3271 1606 Fragments arranged by size: 3271 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5749 AvaI C'yCGr_G Cuts at: 1870 1943 3074 1870 Size: 73 1131 4545 Fragments arranged by size: 4545 1131 73 BamHI G'GATC_C Cuts at: 3079 3446 3079 Size: 367 5382 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3070 4525 618 Size: 325 217 627 1283 1455 1842 Fragments arranged by size: 1842 1455 1283 627 325 217 BanII G_rGCy'C Cuts at: 1391 2199 3068 1391 Size: 808 869 4072 Fragments arranged by size: 4072 869 808 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2710 928 Size: 971 811 3967 Fragments arranged by size: 3967 971 811 BbvCI CC'TCA_GC Cuts at: 2213 2637 2213 Size: 424 5325 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3940 3974 1903 Size: 34 2003 34 3678 Fragments arranged by size: 3678 2003 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3641 4851 3641 Size: 1210 4539 BclI T'GATC_A Cuts at: 1548 1548 Size: 5749 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4360 136 Size: 107 122 71 631 605 2688 1525 Fragments arranged by size: 2688 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5744 5744 Size: 5749 BlpI GC'TnA_GC Cuts at: 3166 3166 Size: 5749 Bme1580I G_kGCm'C Cuts at: 1790 2314 3810 4966 1790 Size: 524 1496 1156 2573 Fragments arranged by size: 2573 1496 1156 524 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5749 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2080 3480 4438 Size: 416 53 83 399 24 663 1400 958 Cuts at: 4438 442 Size: 1753 Fragments arranged by size: 1753 1400 958 663 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5749 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2349 2372 2381 2519 4409 1239 Size: 1110 23 9 138 1890 2579 Fragments arranged by size: 2579 1890 1110 138 23 9 Bpu10I CC'TnA_GC Cuts at: 2213 2273 2637 2213 Size: 60 364 5325 Fragments arranged by size: 5325 364 60 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3874 4739 5037 3874 Size: 865 298 4586 Fragments arranged by size: 4586 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4412 882 Size: 617 2913 2219 Fragments arranged by size: 2913 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3540 493 Size: 711 2336 2702 Fragments arranged by size: 2702 2336 711 BsaBI GATnn'nnATC Cuts at: 5743 5743 Size: 5749 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3556 3938 4573 275 Size: 53 83 186 936 2023 382 635 1451 Fragments arranged by size: 2023 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4182 4580 4854 5001 5412 4182 Size: 398 274 147 411 4519 Fragments arranged by size: 4519 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5524 Fragments arranged by size: 5524 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2348 2625 1186 Size: 1162 277 4310 Fragments arranged by size: 4310 1162 277 BseYI C'CCAG_C Cuts at: 2184 2493 2510 4952 5448 2184 Size: 309 17 2442 496 2485 Fragments arranged by size: 2485 2442 496 309 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2119 1163 Size: 573 383 4793 Fragments arranged by size: 4793 573 383 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3199 3960 4988 1540 Size: 275 186 1198 761 1028 2301 Fragments arranged by size: 2301 1198 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2199 2314 3810 3895 4966 2199 Size: 115 1496 85 1071 2982 Fragments arranged by size: 2982 1496 1071 115 85 BsmI GAATG_Cn' Cuts at: 2228 2277 3260 3353 2228 Size: 49 983 93 4624 Fragments arranged by size: 4624 983 93 49 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2199 2314 3068 3810 3895 4966 1391 Size: 399 409 115 754 742 85 1071 2174 Fragments arranged by size: 2174 1071 754 742 409 399 115 85 BspEI T'CCGG_A Cuts at: 5412 5412 Size: 5749 BspHI T'CATG_A Cuts at: 3638 5368 3638 Size: 1730 4019 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2259 2301 2380 3104 844 Size: 1415 42 79 724 3489 Fragments arranged by size: 3489 1415 724 79 42 BsrBI CCG'CTC Cuts at: 1394 1823 3636 1394 Size: 429 1813 3507 Fragments arranged by size: 3507 1813 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4244 4418 65 Size: 1183 2996 174 1396 Fragments arranged by size: 2996 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4393 4580 1700 Size: 110 2583 187 2869 Fragments arranged by size: 2869 2583 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5749 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5749 BssSI C'ACGA_G Cuts at: 3809 4821 3809 Size: 1012 4737 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5749 BstBI TT'CG_AA Cuts at: 4553 4553 Size: 5749 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5749 BstXI CCAn_nnnn'nTGG Cuts at: 2017 2017 Size: 5749 BstYI r'GATC_y Cuts at: 1972 2356 3079 3446 3831 3848 5300 5386 5398 Size: 384 723 367 385 17 1452 86 12 Cuts at: 5398 5744 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 723 385 384 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2025 2025 Size: 5749 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5197 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5010 Fragments arranged by size: 5010 683 56 BtsI GCAGTG_nn' Cuts at: 2346 3265 4059 4079 2346 Size: 919 794 20 4016 Fragments arranged by size: 4016 919 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5749 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5714 DraI TTT'AAA Cuts at: 3053 3053 Size: 5749 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4756 809 Size: 507 3440 1802 Fragments arranged by size: 3440 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3196 4085 5714 Size: 54 1425 50 275 18 1366 889 1629 Cuts at: 5714 8 Size: 43 Fragments arranged by size: 1629 1425 1366 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3196 1537 Size: 275 1384 4090 Fragments arranged by size: 4090 1384 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2550 3679 1353 Size: 1197 1129 3423 Fragments arranged by size: 3423 1197 1129 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4336 4710 4856 387 Size: 1064 138 2747 374 146 1280 Fragments arranged by size: 2747 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2197 2197 Size: 5749 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2119 2349 2372 2381 2519 2890 3812 Size: 393 487 230 23 9 138 371 922 Cuts at: 3812 4409 5196 5464 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 922 787 597 487 393 371 268 230 138 23 9 EcoNI CCTnn'n_nnAGG Cuts at: 2446 2446 Size: 5749 EcoO109I rG'GnC_Cy Cuts at: 2392 2392 Size: 5749 EcoRI G'AATT_C Cuts at: 2594 3024 3058 3436 2594 Size: 430 34 378 4907 Fragments arranged by size: 4907 430 378 34 FspI TGC'GCA Cuts at: 4255 4255 Size: 5749 HaeII r_GCGC'y Cuts at: 1678 2833 4896 1678 Size: 1155 2063 2531 Fragments arranged by size: 2531 2063 1155 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2072 2104 2663 2695 4390 Size: 32 135 32 500 32 559 32 1695 Cuts at: 4390 4422 4464 4496 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1695 559 500 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3093 3339 669 Size: 988 281 1155 246 3079 Fragments arranged by size: 3079 1155 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5749 HpaI GTT'AAC Cuts at: 3339 3339 Size: 5749 KpnI G_GTAC'C Cuts at: 3074 3074 Size: 5749 MfeI C'AATT_G Cuts at: 3348 3348 Size: 5749 MluI A'CGCG_T Cuts at: 5625 5625 Size: 5749 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4863 5047 1305 Size: 3558 184 2007 Fragments arranged by size: 3558 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3927 Fragments arranged by size: 3927 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2095 2423 2949 3707 4066 4225 518 Size: 648 929 328 526 758 359 159 2042 Fragments arranged by size: 2042 929 758 648 526 359 328 159 MspA1I CmG'CkG Cuts at: 1606 2188 2497 3165 3842 4990 5235 1606 Size: 582 309 668 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 668 582 309 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5197 NdeI CA'TA_TG Cuts at: 387 2455 387 Size: 2068 3681 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5749 NotI GC'GGCC_GC Cuts at: 3196 3196 Size: 5749 NspI r_CATG'y Cuts at: 1132 1229 3107 4652 1132 Size: 97 1878 1545 2229 Fragments arranged by size: 2229 1878 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5749 PciI A'CATG_T Cuts at: 4648 4648 Size: 5749 PflMI CCAn_nnn'nTGG Cuts at: 1108 2327 1108 Size: 1219 4530 PmeI GTTT'AAAC Cuts at: 3053 3053 Size: 5749 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3818 3850 5331 5363 3818 Size: 32 1481 32 4204 Fragments arranged by size: 4204 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2392 2392 Size: 5749 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5749 PsiI TTA'TAA Cuts at: 3319 3319 Size: 5749 PstI C_TGCA'G Cuts at: 830 3101 830 Size: 2271 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5749 PvuII CAG'CTG Cuts at: 2188 2497 2188 Size: 309 5440 SacI G_AGCT'C Cuts at: 2199 2199 Size: 5749 SalI G'TCGA_C Cuts at: 1936 3091 1936 Size: 1155 4594 SbfI CC_TGCA'GG Cuts at: 3101 3101 Size: 5749 ScaI AGT'ACT Cuts at: 3997 5709 3997 Size: 1712 4037 SfcI C'TryA_G Cuts at: 826 1045 2122 3097 4232 4913 5104 826 Size: 219 1077 975 1135 681 191 1471 Fragments arranged by size: 1471 1135 1077 975 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5749 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5749 SmaI CCC'GGG Cuts at: 1872 3076 1872 Size: 1204 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3853 4754 5016 820 Size: 197 926 1910 901 262 1553 Fragments arranged by size: 1910 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3540 493 Size: 3047 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5749 SphI G_CATG'C Cuts at: 1132 3107 1132 Size: 1975 3774 SspI AAT'ATT Cuts at: 5 52 3673 5 Size: 47 3621 2081 Fragments arranged by size: 3621 2081 47 StuI AGG'CCT Cuts at: 1517 2986 1517 Size: 1469 4280 StyI C'CwwG_G Cuts at: 513 1065 2232 2442 2599 3188 513 Size: 552 1167 210 157 589 3074 Fragments arranged by size: 3074 1167 589 552 210 157 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2293 3776 3793 3946 4131 4576 325 Size: 1255 713 1483 17 153 185 445 1498 Fragments arranged by size: 1498 1483 1255 713 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2857 3995 5707 Size: 274 80 33 51 1443 880 1138 1712 Cuts at: 5707 96 Size: 138 Fragments arranged by size: 1712 1443 1138 880 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2454 2486 5709 5741 2454 Size: 32 3223 32 2462 Fragments arranged by size: 3223 2462 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5749 XbaI T'CTAG_A Cuts at: 3085 3085 Size: 5749 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2324 2324 Size: 5749 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5749 XmaI C'CCGG_G Cuts at: 1870 3074 1870 Size: 1204 4545 XmnI GAAnn'nnTTC Cuts at: 2735 2903 3878 2735 Size: 168 975 4606 Fragments arranged by size: 4606 975 168 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3557 276 Size: 53 83 186 936 2023 2468 Fragments arranged by size: 2468 2023 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI