Restriction map for
pFN21AB7685
(Linear) (Six-base) MAPSORT of: KIBB7685.seq Check: 6139 from: 1 to: 1044
KIBB7685.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 885 1044
Size: 114 771 159
Fragments arranged by size:
771 159 114
AfeI AGC'GCT
Cuts at: 0 826 1044
Size: 826 218
AleI CACnn'nnGTG
Cuts at: 0 90 944 1044
Size: 90 854 100
Fragments arranged by size:
854 100 90
AlwNI CAG_nnn'CTG
Cuts at: 0 207 267 322 1044
Size: 207 60 55 722
Fragments arranged by size:
722 207 60 55
ApaLI G'TGCA_C
Cuts at: 0 305 1044
Size: 305 739
ApoI r'AATT_y
Cuts at: 0 589 1019 1044
Size: 589 430 25
Fragments arranged by size:
589 430 25
BanII G_rGCy'C
Cuts at: 0 194 1044
Size: 194 850
BbsI GAAGACnn'nnnn_
Cuts at: 0 705 1044
Size: 705 339
BbvCI CC'TCA_GC
Cuts at: 0 208 632 1044
Size: 208 424 412
Fragments arranged by size:
424 412 208
Bme1580I G_kGCm'C
Cuts at: 0 309 1044
Size: 309 735
BmrI ACTGGGnnnn_n'
Cuts at: 0 75 1044
Size: 75 969
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 344 367 376 514 1044
Size: 344 23 9 138 530
Fragments arranged by size:
530 344 138 23 9
Bpu10I CC'TnA_GC
Cuts at: 0 208 268 632 1044
Size: 208 60 364 412
Fragments arranged by size:
412 364 208 60
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 343 620 1044
Size: 343 277 424
Fragments arranged by size:
424 343 277
BseYI C'CCAG_C
Cuts at: 0 179 488 505 1044
Size: 179 309 17 539
Fragments arranged by size:
539 309 179 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 1044
Size: 114 930
BsiHKAI G_wGCw'C
Cuts at: 0 194 309 1044
Size: 194 115 735
Fragments arranged by size:
735 194 115
BsmI GAATG_Cn'
Cuts at: 0 223 272 1044
Size: 223 49 772
Fragments arranged by size:
772 223 49
Bsp1286I G_dGCh'C
Cuts at: 0 194 309 1044
Size: 194 115 735
Fragments arranged by size:
735 194 115
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 254 296 375 1044
Size: 254 42 79 669
Fragments arranged by size:
669 254 79 42
BstXI CCAn_nnnn'nTGG
Cuts at: 0 12 1044
Size: 12 1032
BstYI r'GATC_y
Cuts at: 0 351 1044
Size: 351 693
Bsu36I CC'TnA_GG
Cuts at: 0 20 1044
Size: 20 1024
BtsI GCAGTG_nn'
Cuts at: 0 341 1044
Size: 341 703
EarI CTCTTCn'nnn_
Cuts at: 0 545 1044
Size: 545 499
EcoICRI GAG'CTC
Cuts at: 0 192 1044
Size: 192 852
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 344 367 376 514 885 1044
Size: 114 230 23 9 138 371 159
Fragments arranged by size:
371 230 159 138 114 23 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 441 1044
Size: 441 603
EcoO109I rG'GnC_Cy
Cuts at: 0 387 1044
Size: 387 657
EcoRI G'AATT_C
Cuts at: 0 589 1019 1044
Size: 589 430 25
Fragments arranged by size:
589 430 25
HaeII r_GCGC'y
Cuts at: 0 828 1044
Size: 828 216
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 67 99 658 690 1044
Size: 67 32 559 32 354
Fragments arranged by size:
559 354 67 32 32
MslI CAynn'nnrTG
Cuts at: 0 90 418 944 1044
Size: 90 328 526 100
Fragments arranged by size:
526 328 100 90
MspA1I CmG'CkG
Cuts at: 0 183 492 1044
Size: 183 309 552
Fragments arranged by size:
552 309 183
NdeI CA'TA_TG
Cuts at: 0 450 1044
Size: 450 594
PflMI CCAn_nnn'nTGG
Cuts at: 0 322 1044
Size: 322 722
PpuMI rG'GwC_Cy
Cuts at: 0 387 1044
Size: 387 657
PvuII CAG'CTG
Cuts at: 0 183 492 1044
Size: 183 309 552
Fragments arranged by size:
552 309 183
SacI G_AGCT'C
Cuts at: 0 194 1044
Size: 194 850
SfcI C'TryA_G
Cuts at: 0 117 1044
Size: 117 927
StuI AGG'CCT
Cuts at: 0 981 1044
Size: 981 63
StyI C'CwwG_G
Cuts at: 0 227 437 594 1044
Size: 227 210 157 450
Fragments arranged by size:
450 227 210 157
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 288 1044
Size: 288 756
TatI w'GTAC_w
Cuts at: 0 852 1044
Size: 852 192
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 449 481 1044
Size: 449 32 563
Fragments arranged by size:
563 449 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 319 1044
Size: 319 725
XmnI GAAnn'nnTTC
Cuts at: 0 730 898 1044
Size: 730 168 146
Fragments arranged by size:
730 168 146
Enzymes that do cut:
AcuI AfeI AleI AlwNI ApaLI ApoI BanII BbsI
BbvCI Bme1580I BmrI BpmI Bpu10I BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspMI BstXI BstYI Bsu36I BtsI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I
MslI MspA1I NdeI PflMI PpuMI PvuII SacI SfcI
StuI StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AlfI AloI ApaI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmtI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I BtgI BtgZI ClaI
CspCI DraI DraIII DrdI EaeI EagI EciI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI
Tth111I XbaI XhoI XmaI ZraI