(Circular) (Six-base) MAPSORT of: pFN21AB7849.seq Check: 4692 from: 1 to: 6340 pFN21AB7849 6340 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4150 278 Size: 53 83 186 936 2614 2468 Fragments arranged by size: 2614 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3683 1937 Size: 1746 4594 Acc65I G'GTAC_C Cuts at: 3090 3661 3090 Size: 571 5769 AclI AA'CG_TT Cuts at: 1563 4467 4840 1563 Size: 2904 373 3063 Fragments arranged by size: 3063 2904 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2818 3070 3322 3406 3574 4403 5787 6055 Size: 1186 252 252 84 168 829 1384 268 Cuts at: 6055 1632 Size: 1917 Fragments arranged by size: 1917 1384 1186 829 268 252 252 168 84 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6143 AflIII A'CryG_T Cuts at: 1203 2692 2860 3196 3616 5239 6216 1203 Size: 1489 168 336 420 1623 977 1327 Fragments arranged by size: 1623 1489 1327 977 420 336 168 AgeI A'CCGG_T Cuts at: 5171 5171 Size: 6340 AhdI GACnn_n'nnGTC Cuts at: 5069 5069 Size: 6340 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6340 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6340 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2111 2143 2111 Size: 32 6308 AlwNI CAG_nnn'CTG Cuts at: 2600 4083 5655 2600 Size: 1483 1572 3285 Fragments arranged by size: 3285 1572 1483 ApaLI G'TGCA_C Cuts at: 2396 2434 3530 4397 5553 2396 Size: 38 1096 867 1156 3183 Fragments arranged by size: 3183 1156 1096 867 38 ApoI r'AATT_y Cuts at: 1493 3649 3875 4027 6136 1493 Size: 2156 226 152 2109 1697 Fragments arranged by size: 2156 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4894 160 Size: 872 3862 1606 Fragments arranged by size: 3862 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6340 AvaI C'yCGr_G Cuts at: 1870 1943 3665 1870 Size: 73 1722 4545 Fragments arranged by size: 4545 1722 73 BamHI G'GATC_C Cuts at: 3670 4037 3670 Size: 367 5973 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3090 3661 5116 618 Size: 325 217 627 1303 571 1455 1842 Fragments arranged by size: 1842 1455 1303 627 571 325 217 BanII G_rGCy'C Cuts at: 1391 3659 1391 Size: 2268 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2750 3199 928 Size: 971 851 449 4069 Fragments arranged by size: 4069 971 851 449 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2437 2471 4531 4565 1903 Size: 34 500 34 2060 34 3678 Fragments arranged by size: 3678 2060 500 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4232 5442 4232 Size: 1210 5130 BclI T'GATC_A Cuts at: 1548 1548 Size: 6340 BfrBI ATG'CAT Cuts at: 2726 2726 Size: 6340 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4951 136 Size: 107 122 71 631 605 3279 1525 Fragments arranged by size: 3279 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2471 6335 2471 Size: 3864 2476 BlpI GC'TnA_GC Cuts at: 3757 3757 Size: 6340 Bme1580I G_kGCm'C Cuts at: 1790 2400 2438 3534 4401 5557 1790 Size: 610 38 1096 867 1156 2573 Fragments arranged by size: 2573 1156 1096 867 610 38 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6340 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4071 5029 442 Size: 416 53 83 399 24 2654 958 1753 Fragments arranged by size: 2654 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6340 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2126 2301 2898 2982 3318 3570 3654 5000 Size: 887 175 597 84 336 252 84 1346 Cuts at: 5000 1239 Size: 2579 Fragments arranged by size: 2579 1346 887 597 336 252 175 84 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2392 2572 4465 5330 5628 2392 Size: 180 1893 865 298 3104 Fragments arranged by size: 3104 1893 865 298 180 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2102 2360 2482 3550 5003 882 Size: 617 603 258 122 1068 1453 2219 Fragments arranged by size: 2219 1453 1068 617 603 258 122 BsaAI yAC'GTr Cuts at: 493 1204 2611 2779 2947 3283 3535 4131 493 Size: 711 1407 168 168 336 252 596 2702 Fragments arranged by size: 2702 1407 711 596 336 252 168 168 BsaBI GATnn'nnATC Cuts at: 6334 6334 Size: 6340 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4147 4529 5164 275 Size: 53 83 186 936 2614 382 635 1451 Fragments arranged by size: 2614 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3128 4773 5171 5445 5592 6003 3128 Size: 1645 398 274 147 411 3465 Fragments arranged by size: 3465 1645 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2111 2141 2320 2350 1178 Size: 30 165 30 708 30 179 30 5168 Fragments arranged by size: 5168 708 179 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2095 2920 3088 3256 3340 1186 Size: 909 825 168 168 84 4186 Fragments arranged by size: 4186 909 825 168 168 84 BseYI C'CCAG_C Cuts at: 2323 5543 6039 2323 Size: 3220 496 2624 Fragments arranged by size: 3220 2624 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5767 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3790 4551 5579 1540 Size: 275 186 1789 761 1028 2301 Fragments arranged by size: 2301 1789 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2400 2438 3112 3534 4401 4486 5557 2400 Size: 38 674 422 867 85 1071 3183 Fragments arranged by size: 3183 1071 867 674 422 85 38 BsiWI C'GTAC_G Cuts at: 3393 3393 Size: 6340 BsmI GAATG_Cn' Cuts at: 3077 3126 3572 3851 3944 3077 Size: 49 446 279 93 5473 Fragments arranged by size: 5473 446 279 93 49 BsmBI CGTCTCn'nnnn_ Cuts at: 3021 3021 Size: 6340 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2400 2438 3112 3534 3659 4401 4486 Size: 399 610 38 674 422 125 742 85 Cuts at: 4486 5557 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 674 610 422 399 125 85 38 BspEI T'CCGG_A Cuts at: 6003 6003 Size: 6340 BspHI T'CATG_A Cuts at: 4229 5959 4229 Size: 1730 4610 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2120 3695 844 Size: 1276 1575 3489 Fragments arranged by size: 3489 1575 1276 BsrBI CCG'CTC Cuts at: 1394 1823 3638 4227 1394 Size: 429 1815 589 3507 Fragments arranged by size: 3507 1815 589 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2060 2986 4835 5009 65 Size: 1183 812 926 1849 174 1396 Fragments arranged by size: 1849 1396 1183 926 812 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4984 5171 1700 Size: 110 3174 187 2869 Fragments arranged by size: 3174 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6340 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6340 BssSI C'ACGA_G Cuts at: 4400 5412 4400 Size: 1012 5328 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6340 BstBI TT'CG_AA Cuts at: 3274 5144 3274 Size: 1870 4470 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6340 BstXI CCAn_nnnn'nTGG Cuts at: 2055 2055 Size: 6340 BstYI r'GATC_y Cuts at: 1972 2471 3670 4037 4422 4439 5891 5977 5989 Size: 499 1199 367 385 17 1452 86 12 Cuts at: 5989 6335 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1199 499 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4849 Fragments arranged by size: 4849 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3588 487 Size: 683 56 2362 3239 Fragments arranged by size: 3239 2362 683 56 BtsI GCAGTG_nn' Cuts at: 3161 3581 3856 4650 4670 3161 Size: 420 275 794 20 4831 Fragments arranged by size: 4831 794 420 275 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6340 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3139 3174 3559 3594 529 Size: 35 2575 35 385 35 3275 Fragments arranged by size: 3275 2575 385 35 35 35 DraI TTT'AAA Cuts at: 3644 3644 Size: 6340 DraIII CAC_nnn'GTG Cuts at: 2442 3464 2442 Size: 1022 5318 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5347 809 Size: 507 4031 1802 Fragments arranged by size: 4031 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3787 4676 6305 Size: 54 1425 50 275 18 1957 889 1629 Cuts at: 6305 8 Size: 43 Fragments arranged by size: 1957 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3787 1537 Size: 275 1975 4090 Fragments arranged by size: 4090 1975 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2046 3024 4270 1353 Size: 693 978 1246 3423 Fragments arranged by size: 3423 1246 978 693 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4927 5301 5447 387 Size: 1064 138 3338 374 146 1280 Fragments arranged by size: 3338 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2126 2301 2818 2898 2982 3070 3318 Size: 393 494 175 517 80 84 88 248 Cuts at: 3318 3322 3406 3570 3574 3654 4403 5000 5787 Size: 4 84 164 4 80 749 597 787 Cuts at: 5787 6055 1239 Size: 268 1524 Fragments arranged by size: 1524 787 749 597 517 494 393 268 248 175 164 88 84 84 80 80 4 4 EcoRI G'AATT_C Cuts at: 3649 4027 3649 Size: 378 5962 FspI TGC'GCA Cuts at: 4846 4846 Size: 6340 HaeII r_GCGC'y Cuts at: 1678 5487 1678 Size: 3809 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2228 2260 2847 2879 4981 Size: 32 135 32 656 32 587 32 2102 Cuts at: 4981 5013 5055 5087 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2102 656 587 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3684 3930 669 Size: 988 281 1746 246 3079 Fragments arranged by size: 3079 1746 988 281 246 HindIII A'AGCT_T Cuts at: 748 2661 748 Size: 1913 4427 HpaI GTT'AAC Cuts at: 3930 3930 Size: 6340 KpnI G_GTAC'C Cuts at: 3094 3665 3094 Size: 571 5769 MfeI C'AATT_G Cuts at: 3939 3939 Size: 6340 MluI A'CGCG_T Cuts at: 6216 6216 Size: 6340 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5454 5638 1305 Size: 4149 184 2007 Fragments arranged by size: 4149 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4518 Fragments arranged by size: 4518 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3119 4298 4657 4816 518 Size: 648 1953 1179 359 159 2042 Fragments arranged by size: 2042 1953 1179 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2323 2520 2838 2922 3213 3342 3381 3756 Size: 717 197 318 84 291 129 39 375 Cuts at: 3756 4433 5581 5826 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 717 677 375 318 291 245 197 129 84 39 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4849 Fragments arranged by size: 4849 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6340 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6340 NotI GC'GGCC_GC Cuts at: 3787 3787 Size: 6340 NsiI A_TGCA'T Cuts at: 2728 2728 Size: 6340 NspI r_CATG'y Cuts at: 1132 1229 2696 2730 2864 3200 3368 3452 3620 Size: 97 1467 34 134 336 168 84 168 Cuts at: 3620 3632 3698 5243 1132 Size: 12 66 1545 2229 Fragments arranged by size: 2229 1545 1467 336 168 168 134 97 84 66 34 12 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6340 PciI A'CATG_T Cuts at: 2692 2860 3196 3616 5239 2692 Size: 168 336 420 1623 3793 Fragments arranged by size: 3793 1623 420 336 168 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6340 PmeI GTTT'AAAC Cuts at: 3644 3644 Size: 6340 PmlI CAC'GTG Cuts at: 2947 3283 3535 2947 Size: 336 252 5752 Fragments arranged by size: 5752 336 252 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2111 2143 4409 4441 5922 5954 2111 Size: 32 2266 32 1481 32 2497 Fragments arranged by size: 2497 2266 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6340 PsiI TTA'TAA Cuts at: 3910 3910 Size: 6340 PstI C_TGCA'G Cuts at: 830 2117 2519 3692 830 Size: 1287 402 1173 3478 Fragments arranged by size: 3478 1287 1173 402 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6340 PvuII CAG'CTG Cuts at: 2323 2520 2838 2922 3342 2323 Size: 197 318 84 420 5321 Fragments arranged by size: 5321 420 318 197 84 SalI G'TCGA_C Cuts at: 1936 3682 1936 Size: 1746 4594 SbfI CC_TGCA'GG Cuts at: 2117 3692 2117 Size: 1575 4765 ScaI AGT'ACT Cuts at: 3437 4588 6300 3437 Size: 1151 1712 3477 Fragments arranged by size: 3477 1712 1151 SfcI C'TryA_G Cuts at: 826 1045 2089 2113 2515 2559 3253 3505 3688 Size: 219 1044 24 402 44 694 252 183 Cuts at: 3688 4823 5504 5695 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1044 694 681 402 252 219 191 183 44 24 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6340 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6340 SmaI CCC'GGG Cuts at: 1872 3667 1872 Size: 1795 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2371 2587 4444 5345 5607 820 Size: 197 926 428 216 1857 901 262 1553 Fragments arranged by size: 1857 1553 926 901 428 262 216 197 SnaBI TAC'GTA Cuts at: 493 4131 493 Size: 3638 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6340 SphI G_CATG'C Cuts at: 1132 3698 1132 Size: 2566 3774 SspI AAT'ATT Cuts at: 5 52 4264 5 Size: 47 4212 2081 Fragments arranged by size: 4212 2081 47 StuI AGG'CCT Cuts at: 1517 3167 1517 Size: 1650 4690 StyI C'CwwG_G Cuts at: 513 1065 2004 2130 3779 513 Size: 552 939 126 1649 3074 Fragments arranged by size: 3074 1649 939 552 126 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4367 4384 4537 4722 5167 325 Size: 1255 2787 17 153 185 445 1498 Fragments arranged by size: 2787 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3435 3485 4586 Size: 274 80 33 51 1443 1458 50 1101 Cuts at: 4586 6298 96 Size: 1712 138 Fragments arranged by size: 1712 1458 1443 1101 274 138 80 51 50 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2320 2352 6300 6332 2320 Size: 32 3948 32 2328 Fragments arranged by size: 3948 2328 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6340 XbaI T'CTAG_A Cuts at: 3676 3676 Size: 6340 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6340 XmaI C'CCGG_G Cuts at: 1870 3665 1870 Size: 1795 4545 XmnI GAAnn'nnTTC Cuts at: 2236 2831 3167 3335 3419 3587 4469 2236 Size: 595 336 168 84 168 882 4107 Fragments arranged by size: 4107 882 595 336 168 168 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4148 276 Size: 53 83 186 936 2614 2468 Fragments arranged by size: 2614 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI