(Circular) (Six-base) MAPSORT of: pFN21AB7879.seq Check: 7984 from: 1 to: 6430 pFN21AB7879 6430 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2246 2246 Size: 6430 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4240 278 Size: 53 83 186 936 2704 2468 Fragments arranged by size: 2704 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3773 1937 Size: 1836 4594 Acc65I G'GTAC_C Cuts at: 3751 3751 Size: 6430 AclI AA'CG_TT Cuts at: 1563 4557 4930 1563 Size: 2994 373 3063 Fragments arranged by size: 3063 2994 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2650 2752 4493 5877 6145 1632 Size: 1018 102 1741 1384 268 1917 Fragments arranged by size: 1917 1741 1384 1018 268 102 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6233 AflIII A'CryG_T Cuts at: 1203 5329 6306 1203 Size: 4126 977 1327 Fragments arranged by size: 4126 1327 977 AgeI A'CCGG_T Cuts at: 5261 5261 Size: 6430 AhdI GACnn_n'nnGTC Cuts at: 5159 5159 Size: 6430 AleI CACnn'nnGTG Cuts at: 1166 2283 1166 Size: 1117 5313 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6430 AlwNI CAG_nnn'CTG Cuts at: 2015 2359 4173 5745 2015 Size: 344 1814 1572 2700 Fragments arranged by size: 2700 1814 1572 344 ApaLI G'TGCA_C Cuts at: 4487 5643 4487 Size: 1156 5274 ApoI r'AATT_y Cuts at: 1493 2214 2441 3624 3739 3965 4117 6226 1493 Size: 721 227 1183 115 226 152 2109 1697 Fragments arranged by size: 2109 1697 1183 721 227 226 152 115 AseI AT'TA_AT Cuts at: 160 1032 4984 160 Size: 872 3952 1606 Fragments arranged by size: 3952 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6430 AvaI C'yCGr_G Cuts at: 1870 1943 3755 1870 Size: 73 1812 4545 Fragments arranged by size: 4545 1812 73 AvrII C'CTAG_G Cuts at: 2249 2249 Size: 6430 BamHI G'GATC_C Cuts at: 3760 4127 3760 Size: 367 6063 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2025 2197 3751 5206 618 Size: 325 217 627 238 172 1554 1455 1842 Fragments arranged by size: 1842 1554 1455 627 325 238 217 172 BanII G_rGCy'C Cuts at: 1391 3749 1391 Size: 2358 4072 BbeI G_GCGC'C Cuts at: 2029 2029 Size: 6430 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2087 2509 2591 928 Size: 971 188 422 82 4767 Fragments arranged by size: 4767 971 422 188 82 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4621 4655 1903 Size: 34 2684 34 3678 Fragments arranged by size: 3678 2684 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4322 5532 4322 Size: 1210 5220 BclI T'GATC_A Cuts at: 1548 1548 Size: 6430 BfrBI ATG'CAT Cuts at: 2605 2605 Size: 6430 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5041 136 Size: 107 122 71 631 605 3369 1525 Fragments arranged by size: 3369 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6425 6425 Size: 6430 BlpI GC'TnA_GC Cuts at: 3847 3847 Size: 6430 Bme1580I G_kGCm'C Cuts at: 1790 2045 4491 5647 1790 Size: 255 2446 1156 2573 Fragments arranged by size: 2573 2446 1156 255 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6430 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2541 3306 4161 Size: 416 53 83 399 24 1124 765 855 Cuts at: 4161 5119 442 Size: 958 1753 Fragments arranged by size: 1753 1124 958 855 765 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6430 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2262 2262 Size: 6430 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2982 3066 3150 3234 3402 3486 3654 5090 Size: 1743 84 84 84 168 84 168 1436 Cuts at: 5090 1239 Size: 2579 Fragments arranged by size: 2579 1743 1436 168 168 84 84 84 84 Bpu10I CC'TnA_GC Cuts at: 2492 2492 Size: 6430 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4555 5420 5718 4555 Size: 865 298 5267 Fragments arranged by size: 5267 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5093 882 Size: 617 3594 2219 Fragments arranged by size: 3594 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4221 493 Size: 711 3017 2702 Fragments arranged by size: 3017 2702 711 BsaBI GATnn'nnATC Cuts at: 6424 6424 Size: 6430 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2026 4237 4619 5254 Size: 53 83 186 936 493 2211 382 635 Cuts at: 5254 275 Size: 1451 Fragments arranged by size: 2211 1451 936 635 493 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4863 5261 5535 5682 6093 4863 Size: 398 274 147 411 5200 Fragments arranged by size: 5200 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2075 2105 1178 Size: 30 165 30 672 30 5503 Fragments arranged by size: 5503 672 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2099 2207 2915 1186 Size: 913 108 708 4701 Fragments arranged by size: 4701 913 708 108 BseYI C'CCAG_C Cuts at: 2164 5633 6129 2164 Size: 3469 496 2465 Fragments arranged by size: 3469 2465 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2749 3589 1163 Size: 573 1013 840 4004 Fragments arranged by size: 4004 1013 840 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3880 4641 5669 1540 Size: 275 186 1879 761 1028 2301 Fragments arranged by size: 2301 1879 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4491 4576 5647 4491 Size: 85 1071 5274 Fragments arranged by size: 5274 1071 85 BsmI GAATG_Cn' Cuts at: 3488 3656 3941 4034 3488 Size: 168 285 93 5884 Fragments arranged by size: 5884 285 168 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2045 3749 4491 4576 5647 1391 Size: 399 255 1704 742 85 1071 2174 Fragments arranged by size: 2174 1704 1071 742 399 255 85 BspEI T'CCGG_A Cuts at: 6093 6093 Size: 6430 BspHI T'CATG_A Cuts at: 3364 4319 6049 3364 Size: 955 1730 3745 Fragments arranged by size: 3745 1730 955 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2246 3785 844 Size: 1402 1539 3489 Fragments arranged by size: 3489 1539 1402 BsrBI CCG'CTC Cuts at: 1394 1823 4317 1394 Size: 429 2494 3507 Fragments arranged by size: 3507 2494 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4925 5099 65 Size: 1183 3677 174 1396 Fragments arranged by size: 3677 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5074 5261 1700 Size: 110 3264 187 2869 Fragments arranged by size: 3264 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2221 96 Size: 2125 4305 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6430 BssSI C'ACGA_G Cuts at: 4490 5502 4490 Size: 1012 5418 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6430 BstBI TT'CG_AA Cuts at: 5234 5234 Size: 6430 BstEII G'GTnAC_C Cuts at: 1184 3014 1184 Size: 1830 4600 BstYI r'GATC_y Cuts at: 1972 3263 3760 4127 4512 4529 5981 6067 6079 Size: 1291 497 367 385 17 1452 86 12 Cuts at: 6079 6425 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1291 497 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4939 Fragments arranged by size: 4939 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5691 Fragments arranged by size: 5691 683 56 BtsI GCAGTG_nn' Cuts at: 2397 2737 2790 3493 3577 3946 4740 4760 2397 Size: 340 53 703 84 369 794 20 4067 Fragments arranged by size: 4067 794 703 369 340 84 53 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6430 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3223 3258 529 Size: 35 2659 35 3701 Fragments arranged by size: 3701 2659 35 35 DraI TTT'AAA Cuts at: 3734 3734 Size: 6430 DraIII CAC_nnn'GTG Cuts at: 3439 3439 Size: 6430 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5437 809 Size: 507 4121 1802 Fragments arranged by size: 4121 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3877 4766 6395 Size: 54 1425 50 275 18 2047 889 1629 Cuts at: 6395 8 Size: 43 Fragments arranged by size: 2047 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3877 1537 Size: 275 2065 4090 Fragments arranged by size: 4090 2065 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4360 1353 Size: 3007 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5017 5391 5537 387 Size: 1064 138 3428 374 146 1280 Fragments arranged by size: 3428 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2650 2752 2982 3066 3150 3234 3402 Size: 393 1018 102 230 84 84 84 168 Cuts at: 3402 3486 3654 4493 5090 5877 6145 1239 Size: 84 168 839 597 787 268 1524 Fragments arranged by size: 1524 1018 839 787 597 393 268 230 168 168 102 84 84 84 84 EcoNI CCTnn'n_nnAGG Cuts at: 2253 2253 Size: 6430 EcoO109I rG'GnC_Cy Cuts at: 2801 2969 3222 2801 Size: 168 253 6009 Fragments arranged by size: 6009 253 168 EcoRI G'AATT_C Cuts at: 3624 3739 4117 3624 Size: 115 378 5937 Fragments arranged by size: 5937 378 115 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3118 3118 Size: 6430 FspI TGC'GCA Cuts at: 4936 4936 Size: 6430 HaeII r_GCGC'y Cuts at: 1678 2029 5577 1678 Size: 351 3548 2531 Fragments arranged by size: 3548 2531 351 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2875 2907 3043 3075 3255 Size: 32 135 32 1303 32 136 32 180 Cuts at: 3255 3287 3433 3465 5071 5103 5145 5177 1373 Size: 32 146 32 1606 32 42 32 2626 Fragments arranged by size: 2626 1606 1303 180 146 136 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3774 4020 669 Size: 988 281 1836 246 3079 Fragments arranged by size: 3079 1836 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6430 HpaI GTT'AAC Cuts at: 4020 4020 Size: 6430 KasI G'GCGC_C Cuts at: 2025 2025 Size: 6430 KpnI G_GTAC'C Cuts at: 3755 3755 Size: 6430 MfeI C'AATT_G Cuts at: 3313 4029 3313 Size: 716 5714 MluI A'CGCG_T Cuts at: 6306 6306 Size: 6430 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5544 5728 1305 Size: 4239 184 2007 Fragments arranged by size: 4239 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4608 Fragments arranged by size: 4608 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2283 3117 3397 4388 4747 4906 518 Size: 648 1117 834 280 991 359 159 2042 Fragments arranged by size: 2042 1117 991 834 648 359 280 159 MspA1I CmG'CkG Cuts at: 1606 2012 3846 4523 5671 5916 1606 Size: 406 1834 677 1148 245 2120 Fragments arranged by size: 2120 1834 1148 677 406 245 NarI GG'CG_CC Cuts at: 2026 2026 Size: 6430 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4939 Fragments arranged by size: 4939 939 552 NdeI CA'TA_TG Cuts at: 387 3394 387 Size: 3007 3423 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6430 NotI GC'GGCC_GC Cuts at: 3877 3877 Size: 6430 NsiI A_TGCA'T Cuts at: 2607 2607 Size: 6430 NspI r_CATG'y Cuts at: 1132 1229 2379 3788 5333 1132 Size: 97 1150 1409 1545 2229 Fragments arranged by size: 2229 1545 1409 1150 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6430 PciI A'CATG_T Cuts at: 5329 5329 Size: 6430 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6430 PmeI GTTT'AAAC Cuts at: 3734 3734 Size: 6430 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4499 4531 6012 6044 4499 Size: 32 1481 32 4885 Fragments arranged by size: 4885 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2801 2801 Size: 6430 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6430 PsiI TTA'TAA Cuts at: 3564 4000 3564 Size: 436 5994 PstI C_TGCA'G Cuts at: 830 3782 830 Size: 2952 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6430 SalI G'TCGA_C Cuts at: 1936 3772 1936 Size: 1836 4594 SbfI CC_TGCA'GG Cuts at: 3782 3782 Size: 6430 ScaI AGT'ACT Cuts at: 4678 6390 4678 Size: 1712 4718 SfcI C'TryA_G Cuts at: 826 1045 2446 2577 3778 4913 5594 5785 826 Size: 219 1401 131 1201 1135 681 191 1471 Fragments arranged by size: 1471 1401 1201 1135 681 219 191 131 SfoI GGC'GCC Cuts at: 2027 2027 Size: 6430 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6430 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6430 SmaI CCC'GGG Cuts at: 1872 3757 1872 Size: 1885 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4534 5435 5697 820 Size: 197 926 2591 901 262 1553 Fragments arranged by size: 2591 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4221 493 Size: 3728 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6430 SphI G_CATG'C Cuts at: 1132 3788 1132 Size: 2656 3774 SspI AAT'ATT Cuts at: 5 52 4354 5 Size: 47 4302 2081 Fragments arranged by size: 4302 2081 47 StuI AGG'CCT Cuts at: 1517 3139 3643 3725 1517 Size: 1622 504 82 4222 Fragments arranged by size: 4222 1622 504 82 StyI C'CwwG_G Cuts at: 513 1065 2004 2249 3869 513 Size: 552 939 245 1620 3074 Fragments arranged by size: 3074 1620 939 552 245 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4457 4474 4627 4812 5257 325 Size: 1255 2877 17 153 185 445 1498 Fragments arranged by size: 2877 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2221 4676 6388 Size: 274 80 33 51 1443 244 2455 1712 Cuts at: 6388 96 Size: 138 Fragments arranged by size: 2455 1712 1443 274 244 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2075 2107 2709 2741 6390 6422 2075 Size: 32 602 32 3649 32 2083 Fragments arranged by size: 3649 2083 602 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6430 XbaI T'CTAG_A Cuts at: 3766 3766 Size: 6430 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6430 XmaI C'CCGG_G Cuts at: 1870 3755 1870 Size: 1885 4545 XmnI GAAnn'nnTTC Cuts at: 2702 4559 2702 Size: 1857 4573 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4238 276 Size: 53 83 186 936 2704 2468 Fragments arranged by size: 2704 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbvCI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI