(Circular) (Six-base) MAPSORT of: pFN21AB7833.seq Check: 9521 from: 1 to: 6319 pFN21AB7833 6319 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4129 278 Size: 53 83 186 936 2593 2468 Fragments arranged by size: 2593 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3662 1937 Size: 1725 4594 Acc65I G'GTAC_C Cuts at: 3640 3640 Size: 6319 AclI AA'CG_TT Cuts at: 1563 4446 4819 1563 Size: 2883 373 3063 Fragments arranged by size: 3063 2883 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2371 2455 3409 4382 5766 6034 1632 Size: 739 84 954 973 1384 268 1917 Fragments arranged by size: 1917 1384 973 954 739 268 84 AflII C'TTAA_G Cuts at: 820 1017 2321 820 Size: 197 1304 4818 Fragments arranged by size: 4818 1304 197 AflIII A'CryG_T Cuts at: 1203 5218 6195 1203 Size: 4015 977 1327 Fragments arranged by size: 4015 1327 977 AgeI A'CCGG_T Cuts at: 5150 5150 Size: 6319 AhdI GACnn_n'nnGTC Cuts at: 5048 5048 Size: 6319 AleI CACnn'nnGTG Cuts at: 1166 3132 3468 1166 Size: 1966 336 4017 Fragments arranged by size: 4017 1966 336 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6319 AlwNI CAG_nnn'CTG Cuts at: 2571 4062 5634 2571 Size: 1491 1572 3256 Fragments arranged by size: 3256 1572 1491 ApaLI G'TGCA_C Cuts at: 4376 5532 4376 Size: 1156 5163 ApoI r'AATT_y Cuts at: 1493 2209 2619 2720 2822 2918 3628 3854 4006 Size: 716 410 101 102 96 710 226 152 Cuts at: 4006 6115 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 716 710 410 226 152 102 101 96 AseI AT'TA_AT Cuts at: 160 1032 2181 3177 4873 160 Size: 872 1149 996 1696 1606 Fragments arranged by size: 1696 1606 1149 996 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6319 AvaI C'yCGr_G Cuts at: 1870 1943 3644 1870 Size: 73 1701 4545 Fragments arranged by size: 4545 1701 73 BamHI G'GATC_C Cuts at: 3649 4016 3649 Size: 367 5952 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3640 5095 618 Size: 325 217 627 1853 1455 1842 Fragments arranged by size: 1853 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3638 1391 Size: 2247 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5348 BbvCI CC'TCA_GC Cuts at: 2202 2202 Size: 6319 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4510 4544 1903 Size: 34 2573 34 3678 Fragments arranged by size: 3678 2573 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4211 5421 4211 Size: 1210 5109 BclI T'GATC_A Cuts at: 1548 1548 Size: 6319 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4930 136 Size: 107 122 71 631 605 337 2921 1525 Fragments arranged by size: 2921 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2851 6314 2851 Size: 3463 2856 BlpI GC'TnA_GC Cuts at: 3736 3736 Size: 6319 Bme1580I G_kGCm'C Cuts at: 1790 4380 5536 1790 Size: 2590 1156 2573 Fragments arranged by size: 2590 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6319 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4050 5008 442 Size: 416 53 83 399 24 2633 958 1753 Fragments arranged by size: 2633 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6319 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 2985 3069 4979 1239 Size: 917 829 84 1910 2579 Fragments arranged by size: 2579 1910 917 829 84 Bpu10I CC'TnA_GC Cuts at: 2202 2202 Size: 6319 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2323 2529 2691 4444 5309 5607 2323 Size: 206 162 1753 865 298 3035 Fragments arranged by size: 3035 1753 865 298 206 162 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2629 4982 882 Size: 617 1130 2353 2219 Fragments arranged by size: 2353 2219 1130 617 BsaAI yAC'GTr Cuts at: 493 1204 4110 493 Size: 711 2906 2702 Fragments arranged by size: 2906 2702 711 BsaBI GATnn'nnATC Cuts at: 6313 6313 Size: 6319 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4126 4508 5143 275 Size: 53 83 186 936 2593 382 635 1451 Fragments arranged by size: 2593 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4752 5150 5424 5571 5982 4752 Size: 398 274 147 411 5089 Fragments arranged by size: 5089 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6094 Fragments arranged by size: 6094 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2096 2584 3340 1186 Size: 910 488 756 4165 Fragments arranged by size: 4165 910 756 488 BseYI C'CCAG_C Cuts at: 2012 5522 6018 2012 Size: 3510 496 2313 Fragments arranged by size: 3510 2313 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3321 1163 Size: 573 1585 4161 Fragments arranged by size: 4161 1585 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3769 4530 5558 1540 Size: 275 186 1768 761 1028 2301 Fragments arranged by size: 2301 1768 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4380 4465 5536 4380 Size: 85 1071 5163 Fragments arranged by size: 5163 1071 85 BsmI GAATG_Cn' Cuts at: 3830 3923 3830 Size: 93 6226 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3638 4380 4465 5536 1391 Size: 399 1848 742 85 1071 2174 Fragments arranged by size: 2174 1848 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5982 5982 Size: 6319 BspHI T'CATG_A Cuts at: 4208 5938 4208 Size: 1730 4589 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3674 844 Size: 2830 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4206 1394 Size: 429 2383 3507 Fragments arranged by size: 3507 2383 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4814 4988 65 Size: 1183 3566 174 1396 Fragments arranged by size: 3566 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4963 5150 1700 Size: 110 3153 187 2869 Fragments arranged by size: 3153 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2692 3112 96 Size: 2596 420 3303 Fragments arranged by size: 3303 2596 420 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6319 BssSI C'ACGA_G Cuts at: 4379 5391 4379 Size: 1012 5307 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6319 BstBI TT'CG_AA Cuts at: 5123 5123 Size: 6319 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6319 BstXI CCAn_nnnn'nTGG Cuts at: 2239 2239 Size: 6319 BstYI r'GATC_y Cuts at: 1972 2851 3649 4016 4401 4418 5870 5956 5968 Size: 879 798 367 385 17 1452 86 12 Cuts at: 5968 6314 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 879 798 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4828 Fragments arranged by size: 4828 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5580 Fragments arranged by size: 5580 683 56 BtsI GCAGTG_nn' Cuts at: 2572 3835 4629 4649 2572 Size: 1263 794 20 4242 Fragments arranged by size: 4242 1263 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6319 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2974 3009 529 Size: 35 2410 35 3839 Fragments arranged by size: 3839 2410 35 35 DraI TTT'AAA Cuts at: 3193 3623 3193 Size: 430 5889 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5326 809 Size: 507 4010 1802 Fragments arranged by size: 4010 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3766 4655 6284 Size: 54 1425 50 275 18 1936 889 1629 Cuts at: 6284 8 Size: 43 Fragments arranged by size: 1936 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3766 1537 Size: 275 1954 4090 Fragments arranged by size: 4090 1954 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3103 3190 4249 1353 Size: 1750 87 1059 3423 Fragments arranged by size: 3423 1750 1059 87 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4906 5280 5426 387 Size: 1064 138 3317 374 146 1280 Fragments arranged by size: 3317 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2371 2455 2985 3069 3409 4382 Size: 393 524 215 84 530 84 340 973 Cuts at: 4382 4979 5766 6034 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 973 787 597 530 524 393 340 268 215 84 84 EcoRI G'AATT_C Cuts at: 3628 4006 3628 Size: 378 5941 EcoRV GAT'ATC Cuts at: 2428 2428 Size: 6319 FspI TGC'GCA Cuts at: 4825 4825 Size: 6319 HaeII r_GCGC'y Cuts at: 1678 5466 1678 Size: 3788 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4960 4992 5034 5066 1373 Size: 32 135 32 3388 32 42 32 2626 Fragments arranged by size: 3388 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2022 3239 3365 3663 3909 669 Size: 988 281 84 1217 126 298 246 3079 Fragments arranged by size: 3079 1217 988 298 281 246 126 84 HindIII A'AGCT_T Cuts at: 748 3084 748 Size: 2336 3983 HpaI GTT'AAC Cuts at: 3909 3909 Size: 6319 KpnI G_GTAC'C Cuts at: 3644 3644 Size: 6319 MfeI C'AATT_G Cuts at: 3918 3918 Size: 6319 MluI A'CGCG_T Cuts at: 6195 6195 Size: 6319 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5433 5617 1305 Size: 4128 184 2007 Fragments arranged by size: 4128 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4497 Fragments arranged by size: 4497 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2560 3132 3216 3400 3468 4277 4636 Size: 648 1394 572 84 184 68 809 359 Cuts at: 4636 4795 518 Size: 159 2042 Fragments arranged by size: 2042 1394 809 648 572 359 184 159 84 68 MspA1I CmG'CkG Cuts at: 1606 3735 4412 5560 5805 1606 Size: 2129 677 1148 245 2120 Fragments arranged by size: 2129 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4828 Fragments arranged by size: 4828 939 552 NdeI CA'TA_TG Cuts at: 387 3117 387 Size: 2730 3589 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6319 NotI GC'GGCC_GC Cuts at: 3766 3766 Size: 6319 NspI r_CATG'y Cuts at: 1132 1229 2701 2742 3205 3677 5222 1132 Size: 97 1472 41 463 472 1545 2229 Fragments arranged by size: 2229 1545 1472 472 463 97 41 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6319 PciI A'CATG_T Cuts at: 5218 5218 Size: 6319 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6319 PmeI GTTT'AAAC Cuts at: 3623 3623 Size: 6319 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4388 4420 5901 5933 4388 Size: 32 1481 32 4774 Fragments arranged by size: 4774 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6319 PsiI TTA'TAA Cuts at: 3026 3889 3026 Size: 863 5456 PstI C_TGCA'G Cuts at: 830 3671 830 Size: 2841 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6319 SalI G'TCGA_C Cuts at: 1936 3661 1936 Size: 1725 4594 SbfI CC_TGCA'GG Cuts at: 3671 3671 Size: 6319 ScaI AGT'ACT Cuts at: 4567 6279 4567 Size: 1712 4607 SfcI C'TryA_G Cuts at: 826 1045 2679 2808 3667 4802 5483 5674 826 Size: 219 1634 129 859 1135 681 191 1471 Fragments arranged by size: 1634 1471 1135 859 681 219 191 129 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6319 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6319 SmaI CCC'GGG Cuts at: 1872 3646 1872 Size: 1774 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2302 2321 2544 2706 4423 5324 Size: 197 926 359 19 223 162 1717 901 Cuts at: 5324 5586 820 Size: 262 1553 Fragments arranged by size: 1717 1553 926 901 359 262 223 197 162 19 SnaBI TAC'GTA Cuts at: 493 4110 493 Size: 3617 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6319 SphI G_CATG'C Cuts at: 1132 3677 1132 Size: 2545 3774 SspI AAT'ATT Cuts at: 5 52 4243 5 Size: 47 4191 2081 Fragments arranged by size: 4191 2081 47 StuI AGG'CCT Cuts at: 1517 3310 3441 1517 Size: 1793 131 4395 Fragments arranged by size: 4395 1793 131 StyI C'CwwG_G Cuts at: 513 1065 2004 3758 513 Size: 552 939 1754 3074 Fragments arranged by size: 3074 1754 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2004 2090 3504 4346 4363 4516 4701 Size: 1255 424 86 1414 842 17 153 185 Cuts at: 4701 5146 325 Size: 445 1498 Fragments arranged by size: 1498 1414 1255 842 445 424 185 153 86 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2692 3112 3448 Size: 274 80 33 51 1443 715 420 336 Cuts at: 3448 4565 6277 96 Size: 1117 1712 138 Fragments arranged by size: 1712 1443 1117 715 420 336 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6279 6311 6279 Size: 32 6287 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6319 XbaI T'CTAG_A Cuts at: 3655 3655 Size: 6319 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6319 XmaI C'CCGG_G Cuts at: 1870 3644 1870 Size: 1774 4545 XmnI GAAnn'nnTTC Cuts at: 2750 3002 3254 4448 2750 Size: 252 252 1194 4621 Fragments arranged by size: 4621 1194 252 252 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4127 276 Size: 53 83 186 936 2593 2468 Fragments arranged by size: 2593 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI