(Circular) (Six-base) MAPSORT of: pFN21AB9647.seq Check: 1955 from: 1 to: 6643 pFN21AB9647 6643 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4453 278 Size: 53 83 186 936 2917 2468 Fragments arranged by size: 2917 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3136 3472 3892 3986 1937 Size: 1199 336 420 94 4594 Fragments arranged by size: 4594 1199 420 336 94 Acc65I G'GTAC_C Cuts at: 3964 3964 Size: 6643 AclI AA'CG_TT Cuts at: 1563 4770 5143 1563 Size: 3207 373 3063 Fragments arranged by size: 3207 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2673 2841 3093 3681 3945 4706 6090 6358 Size: 1041 168 252 588 264 761 1384 268 Cuts at: 6358 1632 Size: 1917 Fragments arranged by size: 1917 1384 1041 761 588 268 264 252 168 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6446 AflIII A'CryG_T Cuts at: 1203 5542 6519 1203 Size: 4339 977 1327 Fragments arranged by size: 4339 1327 977 AgeI A'CCGG_T Cuts at: 5474 5474 Size: 6643 AhdI GACnn_n'nnGTC Cuts at: 5372 5372 Size: 6643 AleI CACnn'nnGTG Cuts at: 1166 2670 2838 1166 Size: 1504 168 4971 Fragments arranged by size: 4971 1504 168 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6643 AlwNI CAG_nnn'CTG Cuts at: 4386 5958 4386 Size: 1572 5071 ApaLI G'TGCA_C Cuts at: 2021 4700 5856 2021 Size: 2679 1156 2808 Fragments arranged by size: 2808 2679 1156 ApoI r'AATT_y Cuts at: 1493 2122 2226 2242 2391 2585 2727 2895 3315 Size: 629 104 16 149 194 142 168 420 Cuts at: 3315 3483 3567 3915 3952 4178 4330 6439 1493 Size: 168 84 348 37 226 152 2109 1697 Fragments arranged by size: 2109 1697 629 420 348 226 194 168 168 152 149 142 104 84 37 16 AseI AT'TA_AT Cuts at: 160 1032 5197 160 Size: 872 4165 1606 Fragments arranged by size: 4165 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6643 AvaI C'yCGr_G Cuts at: 1870 1943 3968 1870 Size: 73 2025 4545 Fragments arranged by size: 4545 2025 73 BamHI G'GATC_C Cuts at: 3973 4340 3973 Size: 367 6276 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3964 5419 618 Size: 325 217 627 2177 1455 1842 Fragments arranged by size: 2177 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3962 1391 Size: 2571 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2862 3870 928 Size: 971 963 1008 3701 Fragments arranged by size: 3701 1008 971 963 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4834 4868 1903 Size: 34 2897 34 3678 Fragments arranged by size: 3678 2897 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4535 5745 4535 Size: 1210 5433 BclI T'GATC_A Cuts at: 1548 1548 Size: 6643 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5254 136 Size: 107 122 71 631 605 3582 1525 Fragments arranged by size: 3582 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6638 6638 Size: 6643 BlpI GC'TnA_GC Cuts at: 4060 4060 Size: 6643 Bme1580I G_kGCm'C Cuts at: 1790 2025 3561 4704 5860 1790 Size: 235 1536 1143 1156 2573 Fragments arranged by size: 2573 1536 1156 1143 235 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6643 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4374 5332 442 Size: 416 53 83 399 24 2957 958 1753 Fragments arranged by size: 2957 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6643 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2340 2757 2925 3177 3261 3513 3765 3849 Size: 1101 417 168 252 84 252 252 84 Cuts at: 3849 5303 1239 Size: 1454 2579 Fragments arranged by size: 2579 1454 1101 417 252 252 252 168 84 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4768 5633 5931 4768 Size: 865 298 5480 Fragments arranged by size: 5480 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5306 882 Size: 617 3807 2219 Fragments arranged by size: 3807 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4434 493 Size: 711 3230 2702 Fragments arranged by size: 3230 2702 711 BsaBI GATnn'nnATC Cuts at: 6637 6637 Size: 6643 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4450 4832 5467 275 Size: 53 83 186 936 2917 382 635 1451 Fragments arranged by size: 2917 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5076 5474 5748 5895 6306 5076 Size: 398 274 147 411 5413 Fragments arranged by size: 5413 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6418 Fragments arranged by size: 6418 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6643 BseYI C'CCAG_C Cuts at: 3554 5846 6342 3554 Size: 2292 496 3855 Fragments arranged by size: 3855 2292 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6070 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4093 4854 5882 1540 Size: 275 186 2092 761 1028 2301 Fragments arranged by size: 2301 2092 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2025 4704 4789 5860 2025 Size: 2679 85 1071 2808 Fragments arranged by size: 2808 2679 1071 85 BsmI GAATG_Cn' Cuts at: 4154 4247 4154 Size: 93 6550 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 3561 3962 4704 4789 5860 1391 Size: 399 235 1536 401 742 85 1071 2174 Fragments arranged by size: 2174 1536 1071 742 401 399 235 85 BspEI T'CCGG_A Cuts at: 6306 6306 Size: 6643 BspHI T'CATG_A Cuts at: 4532 6262 4532 Size: 1730 4913 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3998 844 Size: 3154 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4530 1394 Size: 429 2707 3507 Fragments arranged by size: 3507 2707 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5138 5312 65 Size: 1183 3890 174 1396 Fragments arranged by size: 3890 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5287 5474 1700 Size: 110 3477 187 2869 Fragments arranged by size: 3477 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3577 3934 96 Size: 3481 357 2805 Fragments arranged by size: 3481 2805 357 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6643 BssSI C'ACGA_G Cuts at: 4703 5715 4703 Size: 1012 5631 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6643 BstBI TT'CG_AA Cuts at: 5447 5447 Size: 6643 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6643 BstYI r'GATC_y Cuts at: 1972 3973 4340 4725 4742 6194 6280 6292 6638 Size: 2001 367 385 17 1452 86 12 346 Cuts at: 6638 1972 Size: 1977 Fragments arranged by size: 2001 1977 1452 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3137 3473 3137 Size: 336 6307 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6091 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5904 Fragments arranged by size: 5904 683 56 BtsI GCAGTG_nn' Cuts at: 4159 4953 4973 4159 Size: 794 20 5829 Fragments arranged by size: 5829 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6643 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6608 DraI TTT'AAA Cuts at: 2121 3031 3199 3947 2121 Size: 910 168 748 4817 Fragments arranged by size: 4817 910 748 168 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5650 809 Size: 507 4334 1802 Fragments arranged by size: 4334 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4090 4979 6608 Size: 54 1425 50 275 18 2260 889 1629 Cuts at: 6608 8 Size: 43 Fragments arranged by size: 2260 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4090 1537 Size: 275 2278 4090 Fragments arranged by size: 4090 2278 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2649 2817 2985 3069 3657 3921 4573 1353 Size: 1296 168 168 84 588 264 652 3423 Fragments arranged by size: 3423 1296 652 588 264 168 168 84 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5230 5604 5750 387 Size: 1064 138 3641 374 146 1280 Fragments arranged by size: 3641 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2340 2673 2757 2841 2925 3093 3177 Size: 393 708 333 84 84 84 168 84 Cuts at: 3177 3261 3513 3681 3765 3849 3945 4706 5303 Size: 84 252 168 84 84 96 761 597 Cuts at: 5303 6090 6358 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 761 708 597 393 333 268 252 168 168 96 84 84 84 84 84 84 84 EcoRI G'AATT_C Cuts at: 2226 2242 2391 2727 2895 3315 3915 3952 4330 Size: 16 149 336 168 420 600 37 378 Cuts at: 4330 2226 Size: 4539 Fragments arranged by size: 4539 600 420 378 336 168 149 37 16 FspI TGC'GCA Cuts at: 5149 5149 Size: 6643 HaeII r_GCGC'y Cuts at: 1678 5790 1678 Size: 4112 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3447 3479 5284 5316 5358 Size: 32 135 32 1875 32 1805 32 42 Cuts at: 5358 5390 1373 Size: 32 2626 Fragments arranged by size: 2626 1875 1805 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3987 4233 669 Size: 988 281 2049 246 3079 Fragments arranged by size: 3079 2049 988 281 246 HindIII A'AGCT_T Cuts at: 748 2688 2856 3024 3108 3192 3276 3444 3528 Size: 1940 168 168 84 84 84 168 84 Cuts at: 3528 3780 3864 748 Size: 252 84 3527 Fragments arranged by size: 3527 1940 252 168 168 168 84 84 84 84 84 HpaI GTT'AAC Cuts at: 4233 4233 Size: 6643 KpnI G_GTAC'C Cuts at: 3968 3968 Size: 6643 MfeI C'AATT_G Cuts at: 4242 4242 Size: 6643 MluI A'CGCG_T Cuts at: 6519 6519 Size: 6643 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5757 5941 1305 Size: 4452 184 2007 Fragments arranged by size: 4452 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4821 Fragments arranged by size: 4821 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2670 2838 4601 4960 5119 518 Size: 648 1504 168 1763 359 159 2042 Fragments arranged by size: 2042 1763 1504 648 359 168 159 MspA1I CmG'CkG Cuts at: 1606 4059 4736 5884 6129 1606 Size: 2453 677 1148 245 2120 Fragments arranged by size: 2453 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6091 NdeI CA'TA_TG Cuts at: 387 387 Size: 6643 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6643 NotI GC'GGCC_GC Cuts at: 4090 4090 Size: 6643 NspI r_CATG'y Cuts at: 1132 1229 2200 3858 4001 5546 1132 Size: 97 971 1658 143 1545 2229 Fragments arranged by size: 2229 1658 1545 971 143 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6643 PciI A'CATG_T Cuts at: 5542 5542 Size: 6643 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6643 PmeI GTTT'AAAC Cuts at: 3947 3947 Size: 6643 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4712 4744 6225 6257 4712 Size: 32 1481 32 5098 Fragments arranged by size: 5098 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6643 PsiI TTA'TAA Cuts at: 2037 2694 2862 4213 2037 Size: 657 168 1351 4467 Fragments arranged by size: 4467 1351 657 168 PstI C_TGCA'G Cuts at: 830 3995 830 Size: 3165 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6643 SalI G'TCGA_C Cuts at: 1936 3985 1936 Size: 2049 4594 SbfI CC_TGCA'GG Cuts at: 3995 3995 Size: 6643 ScaI AGT'ACT Cuts at: 4891 6603 4891 Size: 1712 4931 SfcI C'TryA_G Cuts at: 826 1045 3991 5126 5807 5998 826 Size: 219 2946 1135 681 191 1471 Fragments arranged by size: 2946 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6643 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6643 SmaI CCC'GGG Cuts at: 1872 3970 1872 Size: 2098 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4747 5648 5910 820 Size: 197 926 2804 901 262 1553 Fragments arranged by size: 2804 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4434 493 Size: 3941 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6643 SphI G_CATG'C Cuts at: 1132 2200 4001 1132 Size: 1068 1801 3774 Fragments arranged by size: 3774 1801 1068 SspI AAT'ATT Cuts at: 5 52 2079 2681 4567 5 Size: 47 2027 602 1886 2081 Fragments arranged by size: 2081 2027 1886 602 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6643 StyI C'CwwG_G Cuts at: 513 1065 4082 513 Size: 552 3017 3074 Fragments arranged by size: 3074 3017 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3276 4670 4687 4840 5025 5470 325 Size: 1255 1696 1394 17 153 185 445 1498 Fragments arranged by size: 1696 1498 1394 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3577 3934 4889 Size: 274 80 33 51 1443 1600 357 955 Cuts at: 4889 6601 96 Size: 1712 138 Fragments arranged by size: 1712 1600 1443 955 357 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6603 6635 6603 Size: 32 6611 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6643 XbaI T'CTAG_A Cuts at: 3979 3979 Size: 6643 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6643 XmaI C'CCGG_G Cuts at: 1870 3968 1870 Size: 2098 4545 XmnI GAAnn'nnTTC Cuts at: 2162 4772 2162 Size: 2610 4033 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4451 276 Size: 53 83 186 936 2917 2468 Fragments arranged by size: 2917 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI