(Circular) (Six-base) MAPSORT of: pFN21AB7805.seq Check: 2217 from: 1 to: 6271 pFN21AB7805 6271 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2028 2028 Size: 6271 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4081 278 Size: 53 83 186 936 2545 2468 Fragments arranged by size: 2545 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3614 1937 Size: 1677 4594 Acc65I G'GTAC_C Cuts at: 3592 3592 Size: 6271 AclI AA'CG_TT Cuts at: 1563 4398 4771 1563 Size: 2835 373 3063 Fragments arranged by size: 3063 2835 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2328 3020 3082 3334 4334 5718 5986 1632 Size: 696 692 62 252 1000 1384 268 1917 Fragments arranged by size: 1917 1384 1000 696 692 268 252 62 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6074 AflIII A'CryG_T Cuts at: 1203 3326 5170 6147 1203 Size: 2123 1844 977 1327 Fragments arranged by size: 2123 1844 1327 977 AgeI A'CCGG_T Cuts at: 5102 5102 Size: 6271 AhdI GACnn_n'nnGTC Cuts at: 5000 5000 Size: 6271 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6271 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6271 AlwNI CAG_nnn'CTG Cuts at: 4014 5586 4014 Size: 1572 4699 ApaI G_GGCC'C Cuts at: 2116 2116 Size: 6271 ApaLI G'TGCA_C Cuts at: 4328 5484 4328 Size: 1156 5115 ApoI r'AATT_y Cuts at: 1493 2598 2628 2712 2796 3048 3132 3216 3300 Size: 1105 30 84 84 252 84 84 84 Cuts at: 3300 3384 3580 3806 3958 6067 1493 Size: 84 196 226 152 2109 1697 Fragments arranged by size: 2109 1697 1105 252 226 196 152 84 84 84 84 84 84 30 AseI AT'TA_AT Cuts at: 160 1032 4825 160 Size: 872 3793 1606 Fragments arranged by size: 3793 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6271 AvaI C'yCGr_G Cuts at: 1870 1943 3596 1870 Size: 73 1653 4545 Fragments arranged by size: 4545 1653 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2768 2801 2768 Size: 33 6238 BamHI G'GATC_C Cuts at: 3601 3968 3601 Size: 367 5904 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3592 5047 618 Size: 325 217 627 1805 1455 1842 Fragments arranged by size: 1842 1805 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2102 2116 2486 2774 3590 1391 Size: 711 14 370 288 816 4072 Fragments arranged by size: 4072 816 711 370 288 14 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2554 928 Size: 971 655 4645 Fragments arranged by size: 4645 971 655 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4462 4496 1903 Size: 34 2525 34 3678 Fragments arranged by size: 3678 2525 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4163 5373 4163 Size: 1210 5061 BclI T'GATC_A Cuts at: 1548 2500 1548 Size: 952 5319 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2111 4882 136 Size: 107 122 71 631 605 439 2771 1525 Fragments arranged by size: 2771 1525 631 605 439 122 107 71 BglII A'GATC_T Cuts at: 6266 6266 Size: 6271 BlpI GC'TnA_GC Cuts at: 2096 3688 2096 Size: 1592 4679 Bme1580I G_kGCm'C Cuts at: 1790 2116 2944 4332 5488 1790 Size: 326 828 1388 1156 2573 Fragments arranged by size: 2573 1388 1156 828 326 BmgBI CAC'GTC Cuts at: 1318 2128 1318 Size: 810 5461 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2171 3318 4002 Size: 416 53 83 399 24 754 1147 684 Cuts at: 4002 4960 442 Size: 958 1753 Fragments arranged by size: 1753 1147 958 754 684 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2376 1056 Size: 1320 4951 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2444 2505 2595 2444 Size: 61 90 6120 Fragments arranged by size: 6120 90 61 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 2259 2402 2826 2910 3078 3162 3246 Size: 917 103 143 424 84 168 84 84 Cuts at: 3246 4931 1239 Size: 1685 2579 Fragments arranged by size: 2579 1685 917 424 168 143 103 84 84 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4396 5261 5559 4396 Size: 865 298 5108 Fragments arranged by size: 5108 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2161 4934 882 Size: 617 662 2773 2219 Fragments arranged by size: 2773 2219 662 617 BsaAI yAC'GTr Cuts at: 493 1204 2784 4062 493 Size: 711 1580 1278 2702 Fragments arranged by size: 2702 1580 1278 711 BsaBI GATnn'nnATC Cuts at: 6265 6265 Size: 6271 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4078 4460 5095 275 Size: 53 83 186 936 2545 382 635 1451 Fragments arranged by size: 2545 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4704 5102 5376 5523 5934 4704 Size: 398 274 147 411 5041 Fragments arranged by size: 5041 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3555 3585 1178 Size: 30 165 30 2152 30 3864 Fragments arranged by size: 3864 2152 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2764 1186 Size: 1578 4693 BseYI C'CCAG_C Cuts at: 2196 5474 5970 2196 Size: 3278 496 2497 Fragments arranged by size: 3278 2497 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2956 1163 Size: 573 1220 4478 Fragments arranged by size: 4478 1220 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3721 4482 5510 1540 Size: 275 186 1720 761 1028 2301 Fragments arranged by size: 2301 1720 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2477 2486 2774 4332 4417 5488 2477 Size: 9 288 1558 85 1071 3260 Fragments arranged by size: 3260 1558 1071 288 85 9 BsmI GAATG_Cn' Cuts at: 2828 3782 3875 2828 Size: 954 93 5224 Fragments arranged by size: 5224 954 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2102 2116 2477 2486 2774 2944 3590 Size: 399 312 14 361 9 288 170 646 Cuts at: 3590 4332 4417 5488 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 646 399 361 312 288 170 85 14 9 BspEI T'CCGG_A Cuts at: 5934 5934 Size: 6271 BspHI T'CATG_A Cuts at: 4160 5890 4160 Size: 1730 4541 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2028 3626 844 Size: 1184 1598 3489 Fragments arranged by size: 3489 1598 1184 BsrBI CCG'CTC Cuts at: 1394 1823 4158 1394 Size: 429 2335 3507 Fragments arranged by size: 3507 2335 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2396 3001 4766 4940 65 Size: 1183 1148 605 1765 174 1396 Fragments arranged by size: 1765 1396 1183 1148 605 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4915 5102 1700 Size: 110 3105 187 2869 Fragments arranged by size: 3105 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2118 96 Size: 2022 4249 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6271 BssSI C'ACGA_G Cuts at: 4331 5343 4331 Size: 1012 5259 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6271 BstBI TT'CG_AA Cuts at: 5075 5075 Size: 6271 BstEII G'GTnAC_C Cuts at: 1184 2044 1184 Size: 860 5411 BstYI r'GATC_y Cuts at: 1972 3601 3968 4353 4370 5822 5908 5920 6266 Size: 1629 367 385 17 1452 86 12 346 Cuts at: 6266 1972 Size: 1977 Fragments arranged by size: 1977 1629 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2028 513 Size: 552 939 24 4756 Fragments arranged by size: 4756 939 552 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5532 Fragments arranged by size: 5532 683 56 BtsI GCAGTG_nn' Cuts at: 3787 4581 4601 3787 Size: 794 20 5457 Fragments arranged by size: 5457 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6271 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2815 2850 2983 3018 529 Size: 35 2251 35 133 35 3782 Fragments arranged by size: 3782 2251 133 35 35 35 DraI TTT'AAA Cuts at: 2905 3575 2905 Size: 670 5601 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5278 809 Size: 507 3962 1802 Fragments arranged by size: 3962 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3718 4607 6236 Size: 54 1425 50 275 18 1888 889 1629 Cuts at: 6236 8 Size: 43 Fragments arranged by size: 1888 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3718 1537 Size: 275 1906 4090 Fragments arranged by size: 4090 1906 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2076 2496 3115 4201 1353 Size: 723 420 619 1086 3423 Fragments arranged by size: 3423 1086 723 619 420 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4858 5232 5378 387 Size: 1064 138 3269 374 146 1280 Fragments arranged by size: 3269 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2484 2772 2484 Size: 288 5983 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2259 2328 2402 2826 2910 3020 Size: 393 524 103 69 74 424 84 110 Cuts at: 3020 3078 3082 3162 3246 3334 4334 4931 5718 Size: 58 4 80 84 88 1000 597 787 Cuts at: 5718 5986 1239 Size: 268 1524 Fragments arranged by size: 1524 1000 787 597 524 424 393 268 110 103 88 84 84 80 74 69 58 4 EcoO109I rG'GnC_Cy Cuts at: 2112 2113 2174 2112 Size: 1 61 6209 Fragments arranged by size: 6209 61 1 EcoRI G'AATT_C Cuts at: 2712 3048 3132 3300 3384 3580 3958 2712 Size: 336 84 168 84 196 378 5025 Fragments arranged by size: 5025 378 336 196 168 84 84 FspI TGC'GCA Cuts at: 4777 4777 Size: 6271 HaeII r_GCGC'y Cuts at: 1678 5418 1678 Size: 3740 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3179 3211 3445 3477 4912 Size: 32 135 32 1607 32 234 32 1435 Cuts at: 4912 4944 4986 5018 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1607 1435 234 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3615 3861 669 Size: 988 281 1677 246 3079 Fragments arranged by size: 3079 1677 988 281 246 HindIII A'AGCT_T Cuts at: 748 2841 3177 3513 748 Size: 2093 336 336 3506 Fragments arranged by size: 3506 2093 336 336 HpaI GTT'AAC Cuts at: 3861 3861 Size: 6271 KpnI G_GTAC'C Cuts at: 3596 3596 Size: 6271 MfeI C'AATT_G Cuts at: 3870 3870 Size: 6271 MluI A'CGCG_T Cuts at: 6147 6147 Size: 6271 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5385 5569 1305 Size: 4080 184 2007 Fragments arranged by size: 4080 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4449 Fragments arranged by size: 4449 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4229 4588 4747 518 Size: 648 3063 359 159 2042 Fragments arranged by size: 3063 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2095 3687 4364 5512 5757 1606 Size: 489 1592 677 1148 245 2120 Fragments arranged by size: 2120 1592 1148 677 489 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2028 513 Size: 552 939 24 4756 Fragments arranged by size: 4756 939 552 24 NdeI CA'TA_TG Cuts at: 387 3335 387 Size: 2948 3323 NheI G'CTAG_C Cuts at: 1052 2372 1052 Size: 1320 4951 NotI GC'GGCC_GC Cuts at: 3718 3718 Size: 6271 NspI r_CATG'y Cuts at: 1132 1229 3330 3629 5174 1132 Size: 97 2101 299 1545 2229 Fragments arranged by size: 2229 2101 1545 299 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6271 PciI A'CATG_T Cuts at: 3326 5170 3326 Size: 1844 4427 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6271 PfoI T'CCnGG_A Cuts at: 2034 2468 2034 Size: 434 5837 PmeI GTTT'AAAC Cuts at: 3575 3575 Size: 6271 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4340 4372 5853 5885 4340 Size: 32 1481 32 4726 Fragments arranged by size: 4726 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2174 2174 Size: 6271 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6271 PsiI TTA'TAA Cuts at: 3156 3841 3156 Size: 685 5586 PspOMI G'GGCC_C Cuts at: 2112 2112 Size: 6271 PstI C_TGCA'G Cuts at: 830 3623 830 Size: 2793 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6271 PvuII CAG'CTG Cuts at: 2095 2095 Size: 6271 SacI G_AGCT'C Cuts at: 2486 2774 2486 Size: 288 5983 SalI G'TCGA_C Cuts at: 1936 3613 1936 Size: 1677 4594 SbfI CC_TGCA'GG Cuts at: 3623 3623 Size: 6271 ScaI AGT'ACT Cuts at: 4519 6231 4519 Size: 1712 4559 SexAI A'CCwGG_T Cuts at: 2151 2151 Size: 6271 SfcI C'TryA_G Cuts at: 826 1045 2143 3619 4754 5435 5626 826 Size: 219 1098 1476 1135 681 191 1471 Fragments arranged by size: 1476 1471 1135 1098 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6271 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6271 SmaI CCC'GGG Cuts at: 1872 3598 1872 Size: 1726 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4375 5276 5538 820 Size: 197 926 2432 901 262 1553 Fragments arranged by size: 2432 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2784 4062 493 Size: 2291 1278 2702 Fragments arranged by size: 2702 2291 1278 SpeI A'CTAG_T Cuts at: 152 152 Size: 6271 SphI G_CATG'C Cuts at: 1132 3629 1132 Size: 2497 3774 SspI AAT'ATT Cuts at: 5 52 4195 5 Size: 47 4143 2081 Fragments arranged by size: 4143 2081 47 StuI AGG'CCT Cuts at: 1517 2759 3095 1517 Size: 1242 336 4693 Fragments arranged by size: 4693 1242 336 StyI C'CwwG_G Cuts at: 513 1065 2004 2028 3710 513 Size: 552 939 24 1682 3074 Fragments arranged by size: 3074 1682 939 552 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2090 4298 4315 4468 4653 5098 325 Size: 1255 510 2208 17 153 185 445 1498 Fragments arranged by size: 2208 1498 1255 510 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2118 3023 4517 Size: 274 80 33 51 1443 141 905 1494 Cuts at: 4517 6229 96 Size: 1712 138 Fragments arranged by size: 1712 1494 1443 905 274 141 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6231 6263 6231 Size: 32 6239 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6271 XbaI T'CTAG_A Cuts at: 3607 3607 Size: 6271 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6271 XmaI C'CCGG_G Cuts at: 1870 3596 1870 Size: 1726 4545 XmnI GAAnn'nnTTC Cuts at: 3095 3179 4400 3095 Size: 84 1221 4966 Fragments arranged by size: 4966 1221 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4079 276 Size: 53 83 186 936 2545 2468 Fragments arranged by size: 2545 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI