Restriction map for
pFN21AB7808
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccII AccIII AcyI
AfaI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII BalI BamHI
BanI BanII BbeI BbvCI BbvI
BcgI BciVI BclI BcnI BfrI
BglI BlpI Bpu1102I BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstEII
BstPI BstUI BstXI Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I FauI FbaI Fnu4HI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HpaI HpaII
ItaI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MspA1I MspI MunI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PflMI PinAI PmeI PshAI PshBI
PstI PvuI PvuII RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TseI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AciI 465 CCGC CCGC(-3/-1)
AclI 1469 AACGTT AA^CGTT
AspHI 163 GAGCAC G[AT]GC[AT]^C
AvrII 345 CCTAGG C^CTAGG
BbrPI 879 CACGTG CAC^GTG
BbsI 563 GAAGAC GAAGAC(2/6)
BlnI 345 CCTAGG C^CTAGG
BmyI 163 GAGCAC G[AGT]GC[ACT]^C
BpuAI 563 GAAGAC GAAGAC(2/6)
BseRI 46 GAGGAG GAGGAG(10/8)
BsiHKAI 163 GAGCAC G[AT]GC[AT]^C
BsiYI 914 CCCACTCTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 914 CCCACTCTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 163 GAGCAC G[AGT]GC[ACT]^C
Bst1107I 899 GTATAC GTA^TAC
BstZ17I 899 GTATAC GTA^TAC
DdeI 634 CTCAG C^T[ACGT]AG
DraII 910 AGGACCC [GA]G^G[ACGT]CC[CT]
EarI 1614 GAAGAG CTCTTC(1/4)
EcoO109I 910 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 345 CCTAGG C^C[AT][AT]GG
HaeIII 343 GGCC GG^CC
HincII 437 GTCAAC GT[CT]^[GA]AC
HindII 437 GTCAAC GT[CT]^[GA]AC
Ksp632I 1614 GAAGAG CTCTTC(1/4)
MwoI 1566 GCCTTCAGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 398 CATATG CA^TATG
NspI 1713 GCATGT [GA]CATG^[CT]
PmaCI 879 CACGTG CAC^GTG
PmlI 879 CACGTG CAC^GTG
PpuMI 910 AGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 1469 AACGTT AA^CGTT
StyI 345 CCTAGG C^C[AT][AT]GG
TfiI 828 GATTC G^A[AT]TC
Tsp45I 380 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 815 GTAGAC GT^[AC][GT]AC
899 GTATAC
AflIII 880 ACGTGT A^C[GA][CT]GT
987 ACACGT
BfaI 346 CTAG C^TAG
920 CTAG
BglII 1216 AGATCT A^GATCT
1656 AGATCT
BsmAI 194 GTCTC GTCTC(1/5)
632 GTCTC
BstBI 560 TTCGAA TT^CGAA
1756 TTCGAA
BstF5I 21 GGATG GGATG(2/0)
588 GGATG
Cac8I 1463 GCAAGC GC[ACGT]^[ACGT]GC
1549 GCGAGC
EcoRV 1151 GATATC GAT^ATC
1507 GATATC
EcoT22I 1593 ATGCAT ATGCA^T
1711 ATGCAT
FokI 21 GGATG GGATG(9/13)
588 GGATG
HindIII 293 AAGCTT A^AGCTT
321 AAGCTT
MaeI 346 CTAG C^TAG
920 CTAG
MaeIII 350 GTAAC ^GT[ACGT]AC
380 GTGAC
NsiI 1593 ATGCAT ATGCA^T
1711 ATGCAT
NspV 560 TTCGAA TT^CGAA
1756 TTCGAA
SfaNI 96 GATGC GCATC(5/9)
371 GCATC
SfcI 84 CTACAG C^T[GA][CT]AG
125 CTATAG
SfuI 560 TTCGAA TT^CGAA
1756 TTCGAA
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AcsI AluI AlwI ApoI Asp700I
AvaII BpmI BsaAI BsaJI BsmFI
BsmI BspHI BsrDI BsrI BssKI
BstNI BstYI Cfr13I DpnII EcoRI
EcoRII HinfI HphI MaeII MboI
MboII MflI MnlI MseI MslI
MvaI NdeII NlaIII NlaIV PleI
RcaI Sau3AI Sau96I ScrFI TaqI
Tru9I Tsp509I TspRI TthHB8I XhoII
XmnI