(Circular) (Six-base) MAPSORT of: pFN21AB7808.seq Check: 4666 from: 1 to: 6490 pFN21AB7808 6490 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4300 278 Size: 53 83 186 936 2764 2468 Fragments arranged by size: 2764 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2821 2905 3833 1937 Size: 884 84 928 4594 Fragments arranged by size: 4594 928 884 84 Acc65I G'GTAC_C Cuts at: 3811 3811 Size: 6490 AclI AA'CG_TT Cuts at: 1563 3475 4617 4990 1563 Size: 1912 1142 373 3063 Fragments arranged by size: 3063 1912 1142 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2176 2328 2446 3202 3286 3474 3558 3638 Size: 544 152 118 756 84 188 84 80 Cuts at: 3638 3642 4553 5937 6205 1632 Size: 4 911 1384 268 1917 Fragments arranged by size: 1917 1384 911 756 544 268 188 152 118 84 84 80 4 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6293 AflIII A'CryG_T Cuts at: 1203 2885 2992 5389 6366 1203 Size: 1682 107 2397 977 1327 Fragments arranged by size: 2397 1682 1327 977 107 AgeI A'CCGG_T Cuts at: 5321 5321 Size: 6490 AhdI GACnn_n'nnGTC Cuts at: 5219 5219 Size: 6490 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6490 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6490 AlwNI CAG_nnn'CTG Cuts at: 4233 5805 4233 Size: 1572 4918 ApaLI G'TGCA_C Cuts at: 4547 5703 4547 Size: 1156 5334 ApoI r'AATT_y Cuts at: 1493 2664 3061 3084 3764 3799 4025 4177 6286 Size: 1171 397 23 680 35 226 152 2109 Cuts at: 6286 1493 Size: 1697 Fragments arranged by size: 2109 1697 1171 680 397 226 152 35 23 AseI AT'TA_AT Cuts at: 160 1032 5044 160 Size: 872 4012 1606 Fragments arranged by size: 4012 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6490 AvaI C'yCGr_G Cuts at: 1870 1943 3815 1870 Size: 73 1872 4545 Fragments arranged by size: 4545 1872 73 AvrII C'CTAG_G Cuts at: 2350 2350 Size: 6490 BamHI G'GATC_C Cuts at: 3820 4187 3820 Size: 367 6123 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3811 5266 618 Size: 325 217 627 2024 1455 1842 Fragments arranged by size: 2024 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3809 1391 Size: 2418 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2575 928 Size: 971 676 4843 Fragments arranged by size: 4843 971 676 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4681 4715 1903 Size: 34 2744 34 3678 Fragments arranged by size: 3678 2744 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4382 5592 4382 Size: 1210 5280 BclI T'GATC_A Cuts at: 1548 1548 Size: 6490 BfrBI ATG'CAT Cuts at: 3600 3718 3600 Size: 118 6372 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5101 136 Size: 107 122 71 631 605 3429 1525 Fragments arranged by size: 3429 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3221 3661 6485 3221 Size: 440 2824 3226 Fragments arranged by size: 3226 2824 440 BlpI GC'TnA_GC Cuts at: 3907 3907 Size: 6490 Bme1580I G_kGCm'C Cuts at: 1790 4551 5707 1790 Size: 2761 1156 2573 Fragments arranged by size: 2761 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6490 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2006 2255 4221 Size: 416 53 83 399 24 589 249 1966 Cuts at: 4221 5179 442 Size: 958 1753 Fragments arranged by size: 1966 1753 958 589 416 399 249 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6490 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3473 3473 Size: 6490 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2526 2694 2778 3030 3198 3282 3384 3468 Size: 1287 168 84 252 168 84 102 84 Cuts at: 3468 3554 5150 1239 Size: 86 1596 2579 Fragments arranged by size: 2579 1596 1287 252 168 168 102 86 84 84 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4615 5480 5778 4615 Size: 865 298 5327 Fragments arranged by size: 5327 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5153 882 Size: 617 3654 2219 Fragments arranged by size: 3654 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2886 2995 3685 4281 493 Size: 711 1682 109 690 596 2702 Fragments arranged by size: 2702 1682 711 690 596 109 BsaBI GATnn'nnATC Cuts at: 6484 6484 Size: 6490 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4297 4679 5314 275 Size: 53 83 186 936 2764 382 635 1451 Fragments arranged by size: 2764 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4923 5321 5595 5742 6153 4923 Size: 398 274 147 411 5260 Fragments arranged by size: 5260 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2459 2489 1178 Size: 30 165 30 1056 30 5179 Fragments arranged by size: 5179 1056 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2066 1186 Size: 880 5610 BseYI C'CCAG_C Cuts at: 5693 6189 5693 Size: 496 5994 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5917 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3940 4701 5729 1540 Size: 275 186 1939 761 1028 2301 Fragments arranged by size: 2301 1939 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2172 4551 4636 5707 2172 Size: 2379 85 1071 2955 Fragments arranged by size: 2955 2379 1071 85 BsmI GAATG_Cn' Cuts at: 2879 3470 3602 4001 4094 2879 Size: 591 132 399 93 5275 Fragments arranged by size: 5275 591 399 132 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2172 3809 4551 4636 5707 1391 Size: 399 382 1637 742 85 1071 2174 Fragments arranged by size: 2174 1637 1071 742 399 382 85 BspEI T'CCGG_A Cuts at: 6153 6153 Size: 6490 BspHI T'CATG_A Cuts at: 3160 3328 3516 3768 4379 6109 3160 Size: 168 188 252 611 1730 3541 Fragments arranged by size: 3541 1730 611 252 188 168 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3845 844 Size: 3001 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4377 1394 Size: 429 2554 3507 Fragments arranged by size: 3507 2554 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3209 3565 3649 4985 5159 65 Size: 1183 1961 356 84 1336 174 1396 Fragments arranged by size: 1961 1396 1336 1183 356 174 84 BsrFI r'CCGG_y Cuts at: 1700 1810 5134 5321 1700 Size: 110 3324 187 2869 Fragments arranged by size: 3324 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6490 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6490 BssSI C'ACGA_G Cuts at: 4550 5562 4550 Size: 1012 5478 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6490 BstBI TT'CG_AA Cuts at: 2566 3762 5294 2566 Size: 1196 1532 3762 Fragments arranged by size: 3762 1532 1196 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6490 BstYI r'GATC_y Cuts at: 1972 2810 3221 3661 3745 3820 4187 4572 4589 Size: 838 411 440 84 75 367 385 17 Cuts at: 4589 6041 6127 6139 6485 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 838 440 411 385 367 346 86 84 75 17 12 BstZ17I GTA'TAC Cuts at: 2906 2906 Size: 6490 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4999 Fragments arranged by size: 4999 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5751 Fragments arranged by size: 5751 683 56 BtsI GCAGTG_nn' Cuts at: 2957 3125 4006 4800 4820 2957 Size: 168 881 794 20 4627 Fragments arranged by size: 4627 881 794 168 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6490 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6455 DraI TTT'AAA Cuts at: 3794 3794 Size: 6490 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5497 809 Size: 507 4181 1802 Fragments arranged by size: 4181 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3937 4826 6455 Size: 54 1425 50 275 18 2107 889 1629 Cuts at: 6455 8 Size: 43 Fragments arranged by size: 2107 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3937 1537 Size: 275 2125 4090 Fragments arranged by size: 4090 2125 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3614 4420 1353 Size: 2261 806 3423 Fragments arranged by size: 3423 2261 806 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5077 5451 5597 387 Size: 1064 138 3488 374 146 1280 Fragments arranged by size: 3488 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2176 2328 2446 2526 2694 2778 3030 Size: 393 544 152 118 80 168 84 252 Cuts at: 3030 3198 3202 3282 3286 3384 3468 3474 3554 Size: 168 4 80 4 98 84 6 80 Cuts at: 3554 3558 3638 3642 4553 5150 5937 6205 1239 Size: 4 80 4 911 597 787 268 1524 Fragments arranged by size: 1524 911 787 597 544 393 268 252 168 168 152 118 98 84 84 80 80 80 80 6 4 4 4 4 EcoO109I rG'GnC_Cy Cuts at: 2916 2916 Size: 6490 EcoRI G'AATT_C Cuts at: 2664 3061 3764 3799 4177 2664 Size: 397 703 35 378 4977 Fragments arranged by size: 4977 703 397 378 35 EcoRV GAT'ATC Cuts at: 3158 3514 3158 Size: 356 6134 FspI TGC'GCA Cuts at: 4996 4996 Size: 6490 HaeII r_GCGC'y Cuts at: 1678 5637 1678 Size: 3959 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5131 5163 5205 5237 1373 Size: 32 135 32 3559 32 42 32 2626 Fragments arranged by size: 3559 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2444 3834 4080 669 Size: 988 281 506 1390 246 3079 Fragments arranged by size: 3079 1390 988 506 281 246 HindIII A'AGCT_T Cuts at: 748 2298 2326 748 Size: 1550 28 4912 Fragments arranged by size: 4912 1550 28 HpaI GTT'AAC Cuts at: 4080 4080 Size: 6490 KpnI G_GTAC'C Cuts at: 3815 3815 Size: 6490 MfeI C'AATT_G Cuts at: 4089 4089 Size: 6490 MluI A'CGCG_T Cuts at: 6366 6366 Size: 6490 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5604 5788 1305 Size: 4299 184 2007 Fragments arranged by size: 4299 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4668 Fragments arranged by size: 4668 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2437 2559 2579 2689 2884 4448 4807 Size: 648 1271 122 20 110 195 1564 359 Cuts at: 4807 4966 518 Size: 159 2042 Fragments arranged by size: 2042 1564 1271 648 359 195 159 122 110 20 MspA1I CmG'CkG Cuts at: 1606 3906 4583 5731 5976 1606 Size: 2300 677 1148 245 2120 Fragments arranged by size: 2300 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4999 Fragments arranged by size: 4999 939 552 NdeI CA'TA_TG Cuts at: 387 2404 387 Size: 2017 4473 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6490 NotI GC'GGCC_GC Cuts at: 3937 3937 Size: 6490 NsiI A_TGCA'T Cuts at: 3602 3720 3602 Size: 118 6372 NspI r_CATG'y Cuts at: 1132 1229 3722 3848 5393 1132 Size: 97 2493 126 1545 2229 Fragments arranged by size: 2493 2229 1545 126 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6490 PciI A'CATG_T Cuts at: 5389 5389 Size: 6490 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6490 PfoI T'CCnGG_A Cuts at: 2286 2286 Size: 6490 PmeI GTTT'AAAC Cuts at: 3794 3794 Size: 6490 PmlI CAC'GTG Cuts at: 2886 2886 Size: 6490 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2111 2143 4559 4591 6072 6104 2111 Size: 32 2416 32 1481 32 2497 Fragments arranged by size: 2497 2416 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2916 2916 Size: 6490 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6490 PsiI TTA'TAA Cuts at: 2604 3378 4060 2604 Size: 774 682 5034 Fragments arranged by size: 5034 774 682 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3486 3518 3486 Size: 32 6458 PstI C_TGCA'G Cuts at: 830 3842 830 Size: 3012 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6490 SalI G'TCGA_C Cuts at: 1936 3832 1936 Size: 1896 4594 SbfI CC_TGCA'GG Cuts at: 3842 3842 Size: 6490 ScaI AGT'ACT Cuts at: 4738 6450 4738 Size: 1712 4778 SfcI C'TryA_G Cuts at: 826 1045 2089 2130 3838 4973 5654 5845 826 Size: 219 1044 41 1708 1135 681 191 1471 Fragments arranged by size: 1708 1471 1135 1044 681 219 191 41 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6490 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6490 SmaI CCC'GGG Cuts at: 1872 3817 1872 Size: 1945 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4594 5495 5757 820 Size: 197 926 2651 901 262 1553 Fragments arranged by size: 2651 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4281 493 Size: 3788 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6490 SphI G_CATG'C Cuts at: 1132 3848 1132 Size: 2716 3774 SspI AAT'ATT Cuts at: 5 52 4414 5 Size: 47 4362 2081 Fragments arranged by size: 4362 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6490 StyI C'CwwG_G Cuts at: 513 1065 2004 2350 3929 513 Size: 552 939 346 1579 3074 Fragments arranged by size: 3074 1579 939 552 346 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2060 4517 4534 4687 4872 5317 325 Size: 1255 480 2457 17 153 185 445 1498 Fragments arranged by size: 2457 1498 1255 480 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4736 6448 96 Size: 274 80 33 51 1443 2759 1712 138 Fragments arranged by size: 2759 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6450 6482 6450 Size: 32 6458 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6490 XbaI T'CTAG_A Cuts at: 3826 3826 Size: 6490 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6490 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6490 XmaI C'CCGG_G Cuts at: 1870 3815 1870 Size: 1945 4545 XmnI GAAnn'nnTTC Cuts at: 2627 2664 2832 3084 3215 3299 3571 3655 3739 Size: 37 168 252 131 84 272 84 84 Cuts at: 3739 4619 2627 Size: 880 4498 Fragments arranged by size: 4498 880 272 252 168 131 84 84 84 37 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4298 276 Size: 53 83 186 936 2764 2468 Fragments arranged by size: 2764 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI