Restriction map for
pFN21AB7808
(Linear) (Six-base) MAPSORT of: KIBB7808.seq Check: 4089 from: 1 to: 1785
KIBB7808.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 0 816 900 1785
Size: 816 84 885
Fragments arranged by size:
885 816 84
AclI AA'CG_TT
Cuts at: 0 1470 1785
Size: 1470 315
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 323 441 1197 1281 1469 1553 1633
Size: 171 152 118 756 84 188 84 80
Cuts at: 1633 1637 1785
Size: 4 148
Fragments arranged by size:
756 188 171 152 148 118 84 84
80 4
AflIII A'CryG_T
Cuts at: 0 880 987 1785
Size: 880 107 798
Fragments arranged by size:
880 798 107
ApoI r'AATT_y
Cuts at: 0 659 1056 1079 1759 1785
Size: 659 397 23 680 26
Fragments arranged by size:
680 659 397 26 23
AvrII C'CTAG_G
Cuts at: 0 345 1785
Size: 345 1440
BbsI GAAGACnn'nnnn_
Cuts at: 0 570 1785
Size: 570 1215
BfrBI ATG'CAT
Cuts at: 0 1595 1713 1785
Size: 1595 118 72
Fragments arranged by size:
1595 118 72
BglII A'GATC_T
Cuts at: 0 1216 1656 1785
Size: 1216 440 129
Fragments arranged by size:
1216 440 129
BmrI ACTGGGnnnn_n'
Cuts at: 0 1 250 1785
Size: 1 249 1535
Fragments arranged by size:
1535 249 1
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1468 1785
Size: 1468 317
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 521 689 773 1025 1193 1277 1379 1463
Size: 521 168 84 252 168 84 102 84
Cuts at: 1463 1549 1785
Size: 86 236
Fragments arranged by size:
521 252 236 168 168 102 86 84
84 84
BsaAI yAC'GTr
Cuts at: 0 881 990 1680 1785
Size: 881 109 690 105
Fragments arranged by size:
881 690 109 105
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 454 484 1785
Size: 454 30 1301
Fragments arranged by size:
1301 454 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 61 1785
Size: 61 1724
BsiHKAI G_wGCw'C
Cuts at: 0 167 1785
Size: 167 1618
BsmI GAATG_Cn'
Cuts at: 0 874 1465 1597 1785
Size: 874 591 132 188
Fragments arranged by size:
874 591 188 132
Bsp1286I G_dGCh'C
Cuts at: 0 167 1785
Size: 167 1618
BspHI T'CATG_A
Cuts at: 0 1155 1323 1511 1763 1785
Size: 1155 168 188 252 22
Fragments arranged by size:
1155 252 188 168 22
BsrDI GCAATG_nn'
Cuts at: 0 1204 1560 1644 1785
Size: 1204 356 84 141
Fragments arranged by size:
1204 356 141 84
BstBI TT'CG_AA
Cuts at: 0 561 1757 1785
Size: 561 1196 28
Fragments arranged by size:
1196 561 28
BstYI r'GATC_y
Cuts at: 0 805 1216 1656 1740 1785
Size: 805 411 440 84 45
Fragments arranged by size:
805 440 411 84 45
BstZ17I GTA'TAC
Cuts at: 0 901 1785
Size: 901 884
BtsI GCAGTG_nn'
Cuts at: 0 952 1120 1785
Size: 952 168 665
Fragments arranged by size:
952 665 168
EarI CTCTTCn'nnn_
Cuts at: 0 1609 1785
Size: 1609 176
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 323 441 521 689 773 1025 1193
Size: 171 152 118 80 168 84 252 168
Cuts at: 1193 1197 1277 1281 1379 1463 1469 1549 1553
Size: 4 80 4 98 84 6 80 4
Cuts at: 1553 1633 1637 1785
Size: 80 4 148
Fragments arranged by size:
252 171 168 168 152 148 118 98
84 84 80 80 80 80 6 4
4 4 4
EcoO109I rG'GnC_Cy
Cuts at: 0 911 1785
Size: 911 874
EcoRI G'AATT_C
Cuts at: 0 659 1056 1759 1785
Size: 659 397 703 26
Fragments arranged by size:
703 659 397 26
EcoRV GAT'ATC
Cuts at: 0 1153 1509 1785
Size: 1153 356 276
Fragments arranged by size:
1153 356 276
HincII GTy'rAC
Cuts at: 0 439 1785
Size: 439 1346
HindIII A'AGCT_T
Cuts at: 0 293 321 1785
Size: 293 28 1464
Fragments arranged by size:
1464 293 28
MslI CAynn'nnrTG
Cuts at: 0 432 554 574 684 879 1785
Size: 432 122 20 110 195 906
Fragments arranged by size:
906 432 195 122 110 20
NdeI CA'TA_TG
Cuts at: 0 399 1785
Size: 399 1386
NsiI A_TGCA'T
Cuts at: 0 1597 1715 1785
Size: 1597 118 70
Fragments arranged by size:
1597 118 70
NspI r_CATG'y
Cuts at: 0 1717 1785
Size: 1717 68
PfoI T'CCnGG_A
Cuts at: 0 281 1785
Size: 281 1504
PmlI CAC'GTG
Cuts at: 0 881 1785
Size: 881 904
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 106 138 1785
Size: 106 32 1647
Fragments arranged by size:
1647 106 32
PpuMI rG'GwC_Cy
Cuts at: 0 911 1785
Size: 911 874
PsiI TTA'TAA
Cuts at: 0 599 1373 1785
Size: 599 774 412
Fragments arranged by size:
774 599 412
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1481 1513 1785
Size: 1481 32 272
Fragments arranged by size:
1481 272 32
SfcI C'TryA_G
Cuts at: 0 84 125 1785
Size: 84 41 1660
Fragments arranged by size:
1660 84 41
StyI C'CwwG_G
Cuts at: 0 345 1785
Size: 345 1440
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 55 1785
Size: 55 1730
XmnI GAAnn'nnTTC
Cuts at: 0 622 659 827 1079 1210 1294 1566 1650
Size: 622 37 168 252 131 84 272 84
Cuts at: 1650 1734 1785
Size: 84 51
Fragments arranged by size:
622 272 252 168 131 84 84 84
51 37
Enzymes that do cut:
AccI AclI AcuI AflIII ApoI AvrII BbsI BfrBI
BglII BmrI BplI BpmI BsaAI BsaXI BseRI BsiHKAI
BsmI Bsp1286I BspHI BsrDI BstBI BstYI BstZ17I BtsI
EarI Eco57MI EcoO109I EcoRI EcoRV HincII HindIII MslI
NdeI NsiI NspI PfoI PmlI PpiI PpuMI PsiI
PsrI SfcI StyI TaqII XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbvCI BcgI
BciVI BclI BglI BlpI Bme1580I BmgBI BmtI Bpu10I
BpuEI BsaI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiWI BsmBI BspEI BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstEII BstXI Bsu36I BtgI BtgZI ClaI
CspCI DraI DraIII DrdI EaeI EagI EciI EcoICRI
EcoNI FalI FseI FspI FspAI HaeII Hin4I HpaI
KasI KpnI MfeI MluI MmeI MscI MspA1I NaeI
NarI NcoI NgoMIV NheI NotI NruI PacI PasI
PciI PflMI PmeI PshAI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
TstI Tth111I XbaI XcmI XhoI XmaI ZraI