(Circular) (Six-base) MAPSORT of: pFN21AB7876.seq Check: 1087 from: 1 to: 6619 pFN21AB7876 6619 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4429 278 Size: 53 83 186 936 2893 2468 Fragments arranged by size: 2893 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3962 1937 Size: 2025 4594 Acc65I G'GTAC_C Cuts at: 3940 3940 Size: 6619 AclI AA'CG_TT Cuts at: 1563 4746 5119 1563 Size: 3183 373 3063 Fragments arranged by size: 3183 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2002 2201 2755 3175 3511 4682 6066 6334 Size: 370 199 554 420 336 1171 1384 268 Cuts at: 6334 1632 Size: 1917 Fragments arranged by size: 1917 1384 1171 554 420 370 336 268 199 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6422 AflIII A'CryG_T Cuts at: 1203 5518 6495 1203 Size: 4315 977 1327 Fragments arranged by size: 4315 1327 977 AgeI A'CCGG_T Cuts at: 5450 5450 Size: 6619 AhdI GACnn_n'nnGTC Cuts at: 5348 5348 Size: 6619 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6619 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6619 AlwNI CAG_nnn'CTG Cuts at: 4362 5934 4362 Size: 1572 5047 ApaLI G'TGCA_C Cuts at: 4676 5832 4676 Size: 1156 5463 ApoI r'AATT_y Cuts at: 1493 2151 2501 3549 3928 4154 4306 6415 1493 Size: 658 350 1048 379 226 152 2109 1697 Fragments arranged by size: 2109 1697 1048 658 379 350 226 152 AseI AT'TA_AT Cuts at: 160 1032 5173 160 Size: 872 4141 1606 Fragments arranged by size: 4141 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6619 AvaI C'yCGr_G Cuts at: 1870 1943 3944 1870 Size: 73 2001 4545 Fragments arranged by size: 4545 2001 73 BamHI G'GATC_C Cuts at: 3949 4316 3949 Size: 367 6252 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3940 5395 618 Size: 325 217 627 2153 1455 1842 Fragments arranged by size: 2153 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2389 3458 3901 3938 1391 Size: 998 1069 443 37 4072 Fragments arranged by size: 4072 1069 998 443 37 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5648 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4810 4844 1903 Size: 34 50 34 2789 34 3678 Fragments arranged by size: 3678 2789 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4511 5721 4511 Size: 1210 5409 BclI T'GATC_A Cuts at: 1548 1548 Size: 6619 BfrBI ATG'CAT Cuts at: 3251 3637 3721 3251 Size: 386 84 6149 Fragments arranged by size: 6149 386 84 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5230 136 Size: 107 122 71 631 605 3558 1525 Fragments arranged by size: 3558 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3194 3530 6614 3194 Size: 336 3084 3199 Fragments arranged by size: 3199 3084 336 BlpI GC'TnA_GC Cuts at: 4036 4036 Size: 6619 Bme1580I G_kGCm'C Cuts at: 1790 4680 5836 1790 Size: 2890 1156 2573 Fragments arranged by size: 2890 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6619 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2655 2739 2859 Size: 416 53 83 399 24 1238 84 120 Cuts at: 2859 3027 3363 3911 4350 5308 442 Size: 168 336 548 439 958 1753 Fragments arranged by size: 1753 1238 958 548 439 416 399 336 168 120 84 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6619 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2132 2132 Size: 6619 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2217 2583 3003 3087 3171 3423 3507 3591 Size: 978 366 420 84 84 252 84 84 Cuts at: 3591 3675 3759 3843 5279 1239 Size: 84 84 84 1436 2579 Fragments arranged by size: 2579 1436 978 420 366 252 84 84 84 84 84 84 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4744 5609 5907 4744 Size: 865 298 5456 Fragments arranged by size: 5456 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5282 882 Size: 617 3783 2219 Fragments arranged by size: 3783 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4410 493 Size: 711 3206 2702 Fragments arranged by size: 3206 2702 711 BsaBI GATnn'nnATC Cuts at: 6613 6613 Size: 6619 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4426 4808 5443 275 Size: 53 83 186 936 2893 382 635 1451 Fragments arranged by size: 2893 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5052 5450 5724 5871 6282 5052 Size: 398 274 147 411 5389 Fragments arranged by size: 5389 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2345 2375 1178 Size: 30 165 30 942 30 5422 Fragments arranged by size: 5422 942 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2353 1186 Size: 1167 5452 BseYI C'CCAG_C Cuts at: 5822 6318 5822 Size: 496 6123 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6046 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4069 4830 5858 1540 Size: 275 186 2068 761 1028 2301 Fragments arranged by size: 2301 2068 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2245 3458 4680 4765 5836 2245 Size: 1213 1222 85 1071 3028 Fragments arranged by size: 3028 1222 1213 1071 85 BsmI GAATG_Cn' Cuts at: 2426 2684 2852 2979 3425 3509 4130 4223 2426 Size: 258 168 127 446 84 621 93 4822 Fragments arranged by size: 4822 621 446 258 168 127 93 84 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2245 2389 3458 3901 3938 4680 4765 Size: 399 455 144 1069 443 37 742 85 Cuts at: 4765 5836 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1069 742 455 443 399 144 85 37 BspEI T'CCGG_A Cuts at: 6282 6282 Size: 6619 BspHI T'CATG_A Cuts at: 3133 4508 6238 3133 Size: 1375 1730 3514 Fragments arranged by size: 3514 1730 1375 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3974 844 Size: 3130 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4506 1394 Size: 429 2683 3507 Fragments arranged by size: 3507 2683 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3182 3518 3770 5114 5288 65 Size: 1183 1934 336 252 1344 174 1396 Fragments arranged by size: 1934 1396 1344 1183 336 252 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5263 5450 1700 Size: 110 3453 187 2869 Fragments arranged by size: 3453 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6619 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6619 BssSI C'ACGA_G Cuts at: 4679 5691 4679 Size: 1012 5607 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6619 BstBI TT'CG_AA Cuts at: 2623 3799 5423 2623 Size: 1176 1624 3819 Fragments arranged by size: 3819 1624 1176 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6619 BstYI r'GATC_y Cuts at: 1972 3194 3437 3530 3614 3949 4316 4701 4718 Size: 1222 243 93 84 335 367 385 17 Cuts at: 4718 6170 6256 6268 6614 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1222 385 367 346 335 243 93 86 84 17 12 BtgI C'CryG_G Cuts at: 513 1065 2476 513 Size: 552 1411 4656 Fragments arranged by size: 4656 1411 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5880 Fragments arranged by size: 5880 683 56 BtsI GCAGTG_nn' Cuts at: 2224 2245 3434 3602 3686 3901 4135 4929 4949 Size: 21 1189 168 84 215 234 794 20 Cuts at: 4949 2224 Size: 3894 Fragments arranged by size: 3894 1189 794 234 215 168 84 21 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6619 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3076 3111 3412 3447 3496 3531 529 Size: 35 2512 35 301 35 49 35 3617 Fragments arranged by size: 3617 2512 301 49 35 35 35 35 DraI TTT'AAA Cuts at: 3923 3923 Size: 6619 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5626 809 Size: 507 4310 1802 Fragments arranged by size: 4310 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4066 4955 6584 Size: 54 1425 50 275 18 2236 889 1629 Cuts at: 6584 8 Size: 43 Fragments arranged by size: 2236 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4066 1537 Size: 275 2254 4090 Fragments arranged by size: 4090 2254 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2782 3315 4549 1353 Size: 1429 533 1234 3423 Fragments arranged by size: 3423 1429 1234 533 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5206 5580 5726 387 Size: 1064 138 3617 374 146 1280 Fragments arranged by size: 3617 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3456 3456 Size: 6619 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2002 2201 2217 2583 2755 3003 3087 Size: 393 370 199 16 366 172 248 84 Cuts at: 3087 3171 3175 3423 3507 3511 3591 3675 3759 Size: 84 4 248 84 4 80 84 84 Cuts at: 3759 3843 4682 5279 6066 6334 1239 Size: 84 839 597 787 268 1524 Fragments arranged by size: 1524 839 787 597 393 370 366 268 248 248 199 172 84 84 84 84 84 84 80 16 4 4 EcoRI G'AATT_C Cuts at: 3928 4306 3928 Size: 378 6241 EcoRV GAT'ATC Cuts at: 2991 3131 3467 2991 Size: 140 336 6143 Fragments arranged by size: 6143 336 140 FspI TGC'GCA Cuts at: 5125 5125 Size: 6619 HaeII r_GCGC'y Cuts at: 1678 5766 1678 Size: 4088 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5260 5292 5334 5366 1373 Size: 32 135 32 3688 32 42 32 2626 Fragments arranged by size: 3688 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3963 4209 669 Size: 988 281 2025 246 3079 Fragments arranged by size: 3079 2025 988 281 246 HindIII A'AGCT_T Cuts at: 748 3774 748 Size: 3026 3593 HpaI GTT'AAC Cuts at: 4209 4209 Size: 6619 KpnI G_GTAC'C Cuts at: 3944 3944 Size: 6619 MfeI C'AATT_G Cuts at: 4218 4218 Size: 6619 MluI A'CGCG_T Cuts at: 6495 6495 Size: 6619 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5733 5917 1305 Size: 4428 184 2007 Fragments arranged by size: 4428 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4797 Fragments arranged by size: 4797 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2142 2326 2578 2746 2830 2857 3384 Size: 648 976 184 252 168 84 27 527 Cuts at: 3384 4577 4936 5095 518 Size: 1193 359 159 2042 Fragments arranged by size: 2042 1193 976 648 527 359 252 184 168 159 84 27 MspA1I CmG'CkG Cuts at: 1606 4035 4712 5860 6105 1606 Size: 2429 677 1148 245 2120 Fragments arranged by size: 2429 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6067 NdeI CA'TA_TG Cuts at: 387 2293 2743 2827 387 Size: 1906 450 84 4179 Fragments arranged by size: 4179 1906 450 84 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6619 NotI GC'GGCC_GC Cuts at: 4066 4066 Size: 6619 NruI TCG'CGA Cuts at: 3709 3709 Size: 6619 NsiI A_TGCA'T Cuts at: 3253 3639 3723 3253 Size: 386 84 6149 Fragments arranged by size: 6149 386 84 NspI r_CATG'y Cuts at: 1132 1229 2179 2703 3255 3977 5522 1132 Size: 97 950 524 552 722 1545 2229 Fragments arranged by size: 2229 1545 950 722 552 524 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6619 PciI A'CATG_T Cuts at: 5518 5518 Size: 6619 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6619 PmeI GTTT'AAAC Cuts at: 3923 3923 Size: 6619 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2015 2047 4688 4720 6201 6233 2015 Size: 32 2641 32 1481 32 2401 Fragments arranged by size: 2641 2401 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6619 PsiI TTA'TAA Cuts at: 2493 2661 4189 2493 Size: 168 1528 4923 Fragments arranged by size: 4923 1528 168 PstI C_TGCA'G Cuts at: 830 3971 830 Size: 3141 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6619 SacI G_AGCT'C Cuts at: 3458 3458 Size: 6619 SalI G'TCGA_C Cuts at: 1936 3961 1936 Size: 2025 4594 SapI GCTCTTCn'nnn_ Cuts at: 2782 2782 Size: 6619 SbfI CC_TGCA'GG Cuts at: 3971 3971 Size: 6619 ScaI AGT'ACT Cuts at: 4867 6579 4867 Size: 1712 4907 SfcI C'TryA_G Cuts at: 826 1045 2034 3967 5102 5783 5974 826 Size: 219 989 1933 1135 681 191 1471 Fragments arranged by size: 1933 1471 1135 989 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6619 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6619 SmaI CCC'GGG Cuts at: 1872 3946 1872 Size: 2074 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4723 5624 5886 820 Size: 197 926 2780 901 262 1553 Fragments arranged by size: 2780 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4410 493 Size: 3917 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6619 SphI G_CATG'C Cuts at: 1132 3977 1132 Size: 2845 3774 SspI AAT'ATT Cuts at: 5 52 4543 5 Size: 47 4491 2081 Fragments arranged by size: 4491 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6619 StyI C'CwwG_G Cuts at: 513 1065 4058 513 Size: 552 2993 3074 Fragments arranged by size: 3074 2993 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4646 4663 4816 5001 5446 325 Size: 1255 3066 17 153 185 445 1498 Fragments arranged by size: 3066 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4865 6577 96 Size: 274 80 33 51 1443 2888 1712 138 Fragments arranged by size: 2888 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6579 6611 6579 Size: 32 6587 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6619 XbaI T'CTAG_A Cuts at: 3955 3955 Size: 6619 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6619 XmaI C'CCGG_G Cuts at: 1870 3944 1870 Size: 2074 4545 XmnI GAAnn'nnTTC Cuts at: 2684 2768 3188 3356 4748 2684 Size: 84 420 168 1392 4555 Fragments arranged by size: 4555 1392 420 168 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4427 276 Size: 53 83 186 936 2893 2468 Fragments arranged by size: 2893 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI