Restriction map for
pFN21AB8418
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# NO CUT ENZYMES ( 161 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AgeI
AhdI AlwNI AocI Aor51HI ApaI
AscI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
BspDI BspEI BspHI BspMI BsrBI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI ClaI CpoI
DraII DraIII DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT22I FauI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I KasI
KpnI Ksp632I KspI MaeII MamI
MfeI MluI MroI MspA1I MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I StuI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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Alw44I 376 GTGCAC G^TGCAC
ApaLI 376 GTGCAC G^TGCAC
AseI 667 ATTAAT AT^TAAT
AsnI 667 ATTAAT AT^TAAT
AspHI 376 GTGCAC G[AT]GC[AT]^C
BbsI 121 GTCTTC GAAGAC(2/6)
BbvI 267 GCAGC GCAGC(8/12)
BclI 155 TGATCA T^GATCA
BmyI 376 GTGCAC G[AGT]GC[ACT]^C
BpuAI 121 GTCTTC GAAGAC(2/6)
BsiHKAI 376 GTGCAC G[AT]GC[AT]^C
BsmFI 3 GGGAC GGGAC(10/14)
Bsp1286I 376 GTGCAC G[AGT]GC[ACT]^C
BsrDI 54 GCAATG GCAATG(2/0)
BstYI 279 AGATCC [GA]^GATC[CT]
EcoT14I 184 CCTTGG C^C[AT][AT]GG
FbaI 155 TGATCA T^GATCA
Fnu4HI 267 GCAGC GC^[ACGT]GC
HincII 1251 GTTAAC GT[CT]^[GA]AC
HindII 1251 GTTAAC GT[CT]^[GA]AC
HpaI 1251 GTTAAC GTT^AAC
ItaI 267 GCAGC GC^[ACGT]GC
MaeIII 2383 GTGAC ^GT[ACGT]AC
MflI 279 AGATCC [GA]^GATC[CT]
PshBI 667 ATTAAT AT^TAAT
SexAI 116 ACCTGGT A^CC[AT]GGT
SphI 551 GCATGC GCATG^C
StyI 184 CCTTGG C^C[AT][AT]GG
TseI 267 GCAGC G^C[AT]GC
Tsp45I 2383 GTGAC ^GT[GC]AC
XhoII 279 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflIII 691 ACATGT A^C[GA][CT]GT
1027 ACATGT
AlwI 280 GATCC GGATC(4/5)
2150 GATCC
BalI 26 TGGCCA TGG^CCA
259 TGGCCA
BfaI 565 CTAG C^TAG
758 CTAG
BsaJI 126 CCTGGG C^C[ACGT][ACGT]GG
184 CCTTGG
BsmAI 201 GAGAC GTCTC(1/5)
139 GTCTC
Bsp1407I 333 TGTACA T^GTACA
1114 TGTACA
BspLU11I 691 ACATGT A^CATGT
1027 ACATGT
BsrFI 1139 GCCGGT [GA]^CCGG[CT]
1475 GCCGGT
BsrGI 333 TGTACA T^GTACA
1114 TGTACA
Cac8I 414 GCCAGC GC[ACGT]^[ACGT]GC
551 GCATGC
Cfr10I 1139 GCCGGT [GA]^CCGG[CT]
1475 GCCGGT
DraI 339 TTTAAA TTT^AAA
2059 TTTAAA
EaeI 26 TGGCCA [CT]^GGCC[GA]
259 TGGCCA
HapII 1140 CCGG C^CGG
1476 CCGG
HinfI 1085 GAATC G^A[ACGT]TC
1674 GATTC
HpaII 1140 CCGG C^CGG
1476 CCGG
HphI 1126 GGTGA GGTGA(8/7)
158 TCACC
MaeI 565 CTAG C^TAG
758 CTAG
MluNI 26 TGGCCA TGG^CCA
259 TGGCCA
MscI 26 TGGCCA TGG^CCA
259 TGGCCA
MslI 325 CATAAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
352 CATAAAAGTG
MspI 1140 CCGG C^CGG
1476 CCGG
MwoI 183 GCCTTGGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
261 GCCAGAGCAGC
NlaIV 3 GGGACC GG[ACGT]^[ACGT]CC
189 GGAGCC
PstI 1094 CTGCAG CTGCA^G
1430 CTGCAG
SspBI 333 TGTACA T^GTACA
1114 TGTACA
SspI 432 AATATT AAT^ATT
2318 AATATT
TfiI 1085 GAATC G^A[AT]TC
1674 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AcsI AfaI AluI ApoI AvaII
BpmI BseRI BsiYI BslI BsrI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII EcoRI EcoRII FokI HaeIII
HindIII MboI MboII MnlI MseI
MvaI NdeII NlaIII NspI RsaI
Sau3AI Sau96I ScrFI SfcI Tru9I
Tsp509I TspRI