(Circular) (Six-base) MAPSORT of: pFN21AB8418.seq Check: 4902 from: 1 to: 7132 pFN21AB8418 7132 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4942 278 Size: 53 83 186 936 3406 2468 Fragments arranged by size: 3406 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4475 1937 Size: 2538 4594 Acc65I G'GTAC_C Cuts at: 4453 4453 Size: 7132 AclI AA'CG_TT Cuts at: 1563 5259 5632 1563 Size: 3696 373 3063 Fragments arranged by size: 3696 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4401 5195 6579 6847 1632 Size: 2769 794 1384 268 1917 Fragments arranged by size: 2769 1917 1384 794 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6935 AflIII A'CryG_T Cuts at: 1203 2696 3032 6031 7008 1203 Size: 1493 336 2999 977 1327 Fragments arranged by size: 2999 1493 1327 977 336 AgeI A'CCGG_T Cuts at: 5963 5963 Size: 7132 AhdI GACnn_n'nnGTC Cuts at: 5861 5861 Size: 7132 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7132 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7132 AlwNI CAG_nnn'CTG Cuts at: 4875 6447 4875 Size: 1572 5560 ApaLI G'TGCA_C Cuts at: 2381 5189 6345 2381 Size: 2808 1156 3168 Fragments arranged by size: 3168 2808 1156 ApoI r'AATT_y Cuts at: 1493 2039 2083 2474 2482 2602 2855 2922 3006 Size: 546 44 391 8 120 253 67 84 Cuts at: 3006 3107 3174 3342 3426 3510 3762 3779 3846 Size: 101 67 168 84 84 252 17 67 Cuts at: 3846 4182 4266 4350 4441 4667 4819 6928 1493 Size: 336 84 84 91 226 152 2109 1697 Fragments arranged by size: 2109 1697 546 391 336 253 252 226 168 152 120 101 91 84 84 84 84 84 67 67 67 44 17 8 AseI AT'TA_AT Cuts at: 160 1032 2673 5686 160 Size: 872 1641 3013 1606 Fragments arranged by size: 3013 1641 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7132 AvaI C'yCGr_G Cuts at: 1870 1943 4457 1870 Size: 73 2514 4545 Fragments arranged by size: 4545 2514 73 BamHI G'GATC_C Cuts at: 4462 4829 4462 Size: 367 6765 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4453 5908 618 Size: 325 217 627 2666 1455 1842 Fragments arranged by size: 2666 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4451 1391 Size: 3060 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2119 928 Size: 971 220 5941 Fragments arranged by size: 5941 971 220 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5323 5357 1903 Size: 34 3386 34 3678 Fragments arranged by size: 3678 3386 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5024 6234 5024 Size: 1210 5922 BclI T'GATC_A Cuts at: 1548 2160 1548 Size: 612 6520 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5743 136 Size: 107 122 71 631 605 4071 1525 Fragments arranged by size: 4071 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7127 7127 Size: 7132 BlpI GC'TnA_GC Cuts at: 4549 4549 Size: 7132 Bme1580I G_kGCm'C Cuts at: 1790 2385 5193 6349 1790 Size: 595 2808 1156 2573 Fragments arranged by size: 2808 2573 1156 595 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7132 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2225 4863 5821 Size: 416 53 83 399 24 808 2638 958 Cuts at: 5821 442 Size: 1753 Fragments arranged by size: 2638 1753 958 808 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7132 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4385 4385 Size: 7132 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2069 2192 2700 2868 2952 3036 3288 3372 Size: 830 123 508 168 84 84 252 84 Cuts at: 3372 3624 3708 3792 3876 4044 4128 4212 4380 Size: 252 84 84 84 168 84 84 168 Cuts at: 4380 5792 1239 Size: 1412 2579 Fragments arranged by size: 2579 1412 830 508 252 252 168 168 168 123 84 84 84 84 84 84 84 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5257 6122 6420 5257 Size: 865 298 5969 Fragments arranged by size: 5969 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5795 882 Size: 617 4296 2219 Fragments arranged by size: 4296 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4923 493 Size: 711 3719 2702 Fragments arranged by size: 3719 2702 711 BsaBI GATnn'nnATC Cuts at: 7126 7126 Size: 7132 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4939 5321 5956 275 Size: 53 83 186 936 3406 382 635 1451 Fragments arranged by size: 3406 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5565 5963 6237 6384 6795 5565 Size: 398 274 147 411 5902 Fragments arranged by size: 5902 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6907 Fragments arranged by size: 6907 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2066 2218 2403 3956 1186 Size: 880 152 185 1553 4362 Fragments arranged by size: 4362 1553 880 185 152 BseYI C'CCAG_C Cuts at: 6335 6831 6335 Size: 496 6636 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6559 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4582 5343 6371 1540 Size: 275 186 2581 761 1028 2301 Fragments arranged by size: 2581 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2385 5193 5278 6349 2385 Size: 2808 85 1071 3168 Fragments arranged by size: 3168 2808 1071 85 BsmI GAATG_Cn' Cuts at: 4643 4736 4643 Size: 93 7039 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2385 4451 5193 5278 6349 1391 Size: 399 595 2066 742 85 1071 2174 Fragments arranged by size: 2174 2066 1071 742 595 399 85 BspEI T'CCGG_A Cuts at: 6795 6795 Size: 7132 BspHI T'CATG_A Cuts at: 5021 6751 5021 Size: 1730 5402 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4487 844 Size: 3643 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5019 1394 Size: 429 3196 3507 Fragments arranged by size: 3507 3196 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2066 5627 5801 65 Size: 1183 818 3561 174 1396 Fragments arranged by size: 3561 1396 1183 818 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3144 3480 5776 5963 1700 Size: 110 1334 336 2296 187 2869 Fragments arranged by size: 2869 2296 1334 336 187 110 BsrGI T'GTAC_A Cuts at: 96 2338 3119 96 Size: 2242 781 4109 Fragments arranged by size: 4109 2242 781 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7132 BssSI C'ACGA_G Cuts at: 5192 6204 5192 Size: 1012 6120 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7132 BstBI TT'CG_AA Cuts at: 5936 5936 Size: 7132 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7132 BstYI r'GATC_y Cuts at: 1972 2284 4462 4829 5214 5231 6683 6769 6781 Size: 312 2178 367 385 17 1452 86 12 Cuts at: 6781 7127 1972 Size: 346 1977 Fragments arranged by size: 2178 1977 1452 385 367 346 312 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5641 Fragments arranged by size: 5641 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2056 487 Size: 683 56 830 5563 Fragments arranged by size: 5563 830 683 56 BtsI GCAGTG_nn' Cuts at: 4648 5442 5462 4648 Size: 794 20 6318 Fragments arranged by size: 6318 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7132 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2521 2556 529 Size: 35 1957 35 5105 Fragments arranged by size: 5105 1957 35 35 DraI TTT'AAA Cuts at: 2346 4066 4436 2346 Size: 1720 370 5042 Fragments arranged by size: 5042 1720 370 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6139 809 Size: 507 4823 1802 Fragments arranged by size: 4823 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2031 2264 4579 Size: 54 1425 50 275 18 201 233 2315 Cuts at: 4579 5468 7097 8 Size: 889 1629 43 Fragments arranged by size: 2315 1629 1425 889 275 233 201 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4579 1537 Size: 275 2767 4090 Fragments arranged by size: 4090 2767 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5062 1353 Size: 3709 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5719 6093 6239 387 Size: 1064 138 4130 374 146 1280 Fragments arranged by size: 4130 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2069 2192 2700 2868 2952 3036 3288 Size: 393 437 123 508 168 84 84 252 Cuts at: 3288 3372 3624 3708 3792 3876 4044 4128 4212 Size: 84 252 84 84 84 168 84 84 Cuts at: 4212 4380 4401 5195 5792 6579 6847 1239 Size: 168 21 794 597 787 268 1524 Fragments arranged by size: 1524 794 787 597 508 437 393 268 252 252 168 168 168 123 84 84 84 84 84 84 84 84 21 EcoRI G'AATT_C Cuts at: 2039 3006 3342 3426 4266 4441 4819 2039 Size: 967 336 84 840 175 378 4352 Fragments arranged by size: 4352 967 840 378 336 175 84 FspI TGC'GCA Cuts at: 5638 5638 Size: 7132 HaeII r_GCGC'y Cuts at: 1678 6279 1678 Size: 4601 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2152 2184 2757 2789 4393 Size: 32 135 32 580 32 573 32 1604 Cuts at: 4393 4425 5773 5805 5847 5879 1373 Size: 32 1348 32 42 32 2626 Fragments arranged by size: 2626 1604 1348 580 573 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3258 4476 4722 669 Size: 988 281 1320 1218 246 3079 Fragments arranged by size: 3079 1320 1218 988 281 246 HindIII A'AGCT_T Cuts at: 748 2631 2799 2883 3135 3219 3239 3471 3555 Size: 1883 168 84 252 84 20 232 84 Cuts at: 3555 3575 3639 3723 3807 3891 4059 4143 4311 Size: 20 64 84 84 84 168 84 168 Cuts at: 4311 748 Size: 3569 Fragments arranged by size: 3569 1883 252 232 168 168 168 84 84 84 84 84 84 84 64 20 20 HpaI GTT'AAC Cuts at: 3258 4722 3258 Size: 1464 5668 KpnI G_GTAC'C Cuts at: 4457 4457 Size: 7132 MfeI C'AATT_G Cuts at: 4731 4731 Size: 7132 MluI A'CGCG_T Cuts at: 7008 7008 Size: 7132 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6246 6430 1305 Size: 4941 184 2007 Fragments arranged by size: 4941 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2033 2266 10 Size: 54 1425 343 201 233 4876 Fragments arranged by size: 4876 1425 343 233 201 54 MslI CAynn'nnrTG Cuts at: 518 1166 2334 2361 5090 5449 5608 518 Size: 648 1168 27 2729 359 159 2042 Fragments arranged by size: 2729 2042 1168 648 359 159 27 MspA1I CmG'CkG Cuts at: 1606 4548 5225 6373 6618 1606 Size: 2942 677 1148 245 2120 Fragments arranged by size: 2942 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5641 Fragments arranged by size: 5641 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7132 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7132 NotI GC'GGCC_GC Cuts at: 4579 4579 Size: 7132 NspI r_CATG'y Cuts at: 1132 1229 2560 2700 3036 4490 6035 1132 Size: 97 1331 140 336 1454 1545 2229 Fragments arranged by size: 2229 1545 1454 1331 336 140 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7132 PciI A'CATG_T Cuts at: 2696 3032 6031 2696 Size: 336 2999 3797 Fragments arranged by size: 3797 2999 336 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7132 PfoI T'CCnGG_A Cuts at: 2929 2929 Size: 7132 PmeI GTTT'AAAC Cuts at: 4436 4436 Size: 7132 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5201 5233 6714 6746 5201 Size: 32 1481 32 5587 Fragments arranged by size: 5587 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7132 PsiI TTA'TAA Cuts at: 2397 4702 2397 Size: 2305 4827 PstI C_TGCA'G Cuts at: 830 3103 3439 4484 830 Size: 2273 336 1045 3478 Fragments arranged by size: 3478 2273 1045 336 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7132 SalI G'TCGA_C Cuts at: 1936 4474 1936 Size: 2538 4594 SbfI CC_TGCA'GG Cuts at: 4484 4484 Size: 7132 ScaI AGT'ACT Cuts at: 5380 7092 5380 Size: 1712 5420 SexAI A'CCwGG_T Cuts at: 2121 2121 Size: 7132 SfcI C'TryA_G Cuts at: 826 1045 2113 3099 3435 4480 5615 6296 6487 Size: 219 1068 986 336 1045 1135 681 191 Cuts at: 6487 826 Size: 1471 Fragments arranged by size: 1471 1135 1068 1045 986 681 336 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7132 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7132 SmaI CCC'GGG Cuts at: 1872 4459 1872 Size: 2587 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5236 6137 6399 820 Size: 197 926 3293 901 262 1553 Fragments arranged by size: 3293 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4923 493 Size: 4430 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7132 SphI G_CATG'C Cuts at: 1132 2560 4490 1132 Size: 1428 1930 3774 Fragments arranged by size: 3774 1930 1428 SspI AAT'ATT Cuts at: 5 52 2439 4325 5056 5 Size: 47 2387 1886 731 2081 Fragments arranged by size: 2387 2081 1886 731 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7132 StyI C'CwwG_G Cuts at: 513 1065 2004 2189 4571 513 Size: 552 939 185 2382 3074 Fragments arranged by size: 3074 2382 939 552 185 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5159 5176 5329 5514 5959 325 Size: 1255 3579 17 153 185 445 1498 Fragments arranged by size: 3579 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2338 3119 5378 Size: 274 80 33 51 1443 361 781 2259 Cuts at: 5378 7090 96 Size: 1712 138 Fragments arranged by size: 2259 1712 1443 781 361 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7092 7124 7092 Size: 32 7100 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7132 XbaI T'CTAG_A Cuts at: 4468 4468 Size: 7132 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7132 XmaI C'CCGG_G Cuts at: 1870 4457 1870 Size: 2587 4545 XmnI GAAnn'nnTTC Cuts at: 5261 5261 Size: 7132 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4940 276 Size: 53 83 186 936 3406 2468 Fragments arranged by size: 3406 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI