(Circular) (Six-base) MAPSORT of: pFN21AB5965.seq Check: 7299 from: 1 to: 6433 pFN21AB5965 6433 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3314 3314 Size: 6433 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4243 278 Size: 53 83 186 936 2707 2468 Fragments arranged by size: 2707 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2288 2833 3272 3776 1937 Size: 351 545 439 504 4594 Fragments arranged by size: 4594 545 504 439 351 Acc65I G'GTAC_C Cuts at: 3754 3754 Size: 6433 AclI AA'CG_TT Cuts at: 1563 4560 4933 1563 Size: 2997 373 3063 Fragments arranged by size: 3063 2997 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2885 3368 4496 5880 6148 1632 Size: 1253 483 1128 1384 268 1917 Fragments arranged by size: 1917 1384 1253 1128 483 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6236 AflIII A'CryG_T Cuts at: 1203 5332 6309 1203 Size: 4129 977 1327 Fragments arranged by size: 4129 1327 977 AgeI A'CCGG_T Cuts at: 5264 5264 Size: 6433 AhdI GACnn_n'nnGTC Cuts at: 2212 5162 2212 Size: 2950 3483 AleI CACnn'nnGTG Cuts at: 1166 3108 3628 1166 Size: 1942 520 3971 Fragments arranged by size: 3971 1942 520 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6433 AlwNI CAG_nnn'CTG Cuts at: 2171 4176 5748 2171 Size: 2005 1572 2856 Fragments arranged by size: 2856 2005 1572 ApaLI G'TGCA_C Cuts at: 3129 3263 4490 5646 3129 Size: 134 1227 1156 3916 Fragments arranged by size: 3916 1227 1156 134 ApoI r'AATT_y Cuts at: 1493 3742 3968 4120 6229 1493 Size: 2249 226 152 2109 1697 Fragments arranged by size: 2249 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4987 160 Size: 872 3955 1606 Fragments arranged by size: 3955 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6433 AvaI C'yCGr_G Cuts at: 1870 1943 2577 2618 3022 3758 1870 Size: 73 634 41 404 736 4545 Fragments arranged by size: 4545 736 634 404 73 41 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2998 3031 2998 Size: 33 6400 BamHI G'GATC_C Cuts at: 2916 3763 4130 2916 Size: 847 367 5219 Fragments arranged by size: 5219 847 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2691 3754 5209 618 Size: 325 217 627 904 1063 1455 1842 Fragments arranged by size: 1842 1455 1063 904 627 325 217 BanII G_rGCy'C Cuts at: 1391 3752 1391 Size: 2361 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5462 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4624 4658 1903 Size: 34 2687 34 3678 Fragments arranged by size: 3678 2687 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3026 4325 5535 3026 Size: 1299 1210 3924 Fragments arranged by size: 3924 1299 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6433 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3497 5044 136 Size: 107 122 71 631 605 1825 1547 1525 Fragments arranged by size: 1825 1547 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6428 6428 Size: 6433 BlpI GC'TnA_GC Cuts at: 2847 3850 2847 Size: 1003 5430 Bme1580I G_kGCm'C Cuts at: 1790 2780 2963 3133 3267 4494 5650 1790 Size: 990 183 170 134 1227 1156 2573 Fragments arranged by size: 2573 1227 1156 990 183 170 134 BmgBI CAC'GTC Cuts at: 1318 2376 1318 Size: 1058 5375 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3622 4164 5122 Size: 416 53 83 399 24 2205 542 958 Cuts at: 5122 442 Size: 1753 Fragments arranged by size: 2205 1753 958 542 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6433 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2328 3068 5093 1239 Size: 1089 740 2025 2579 Fragments arranged by size: 2579 2025 1089 740 Bpu10I CC'TnA_GC Cuts at: 2568 3456 3462 2568 Size: 888 6 5539 Fragments arranged by size: 5539 888 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3423 3429 3453 4558 5423 5721 3423 Size: 6 24 1105 865 298 4135 Fragments arranged by size: 4135 1105 865 298 24 6 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2753 2933 5096 882 Size: 617 1254 180 2163 2219 Fragments arranged by size: 2219 2163 1254 617 180 BsaAI yAC'GTr Cuts at: 493 1204 4224 493 Size: 711 3020 2702 Fragments arranged by size: 3020 2702 711 BsaBI GATnn'nnATC Cuts at: 2664 2735 6427 2664 Size: 71 3692 2670 Fragments arranged by size: 3692 2670 71 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4240 4622 5257 275 Size: 53 83 186 936 2707 382 635 1451 Fragments arranged by size: 2707 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4866 5264 5538 5685 6096 4866 Size: 398 274 147 411 5203 Fragments arranged by size: 5203 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6208 Fragments arranged by size: 6208 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6433 BseYI C'CCAG_C Cuts at: 2820 3003 5636 6132 2820 Size: 183 2633 496 3121 Fragments arranged by size: 3121 2633 496 183 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3292 3416 1163 Size: 573 1556 124 4180 Fragments arranged by size: 4180 1556 573 124 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3883 4644 5672 1540 Size: 275 186 1882 761 1028 2301 Fragments arranged by size: 2301 1882 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2705 3133 3267 3720 4494 4579 5650 2705 Size: 428 134 453 774 85 1071 3488 Fragments arranged by size: 3488 1071 774 453 428 134 85 BsmI GAATG_Cn' Cuts at: 3944 4037 3944 Size: 93 6340 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2705 2780 2963 3133 3267 3720 3752 Size: 399 915 75 183 170 134 453 32 Cuts at: 3752 4494 4579 5650 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 915 742 453 399 183 170 134 85 75 32 BspEI T'CCGG_A Cuts at: 6096 6096 Size: 6433 BspHI T'CATG_A Cuts at: 4322 6052 4322 Size: 1730 4703 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2470 3314 3788 844 Size: 1626 844 474 3489 Fragments arranged by size: 3489 1626 844 474 BsrBI CCG'CTC Cuts at: 1394 1823 4320 1394 Size: 429 2497 3507 Fragments arranged by size: 3507 2497 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2782 4928 5102 65 Size: 1183 1534 2146 174 1396 Fragments arranged by size: 2146 1534 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5077 5264 1700 Size: 110 3267 187 2869 Fragments arranged by size: 3267 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3300 96 Size: 3204 3229 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6433 BssSI C'ACGA_G Cuts at: 4493 5505 4493 Size: 1012 5421 BstAPI GCAn_nnn'nTGC Cuts at: 794 3315 3394 794 Size: 2521 79 3833 Fragments arranged by size: 3833 2521 79 BstBI TT'CG_AA Cuts at: 5237 5237 Size: 6433 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6433 BstXI CCAn_nnnn'nTGG Cuts at: 3572 3712 3572 Size: 140 6293 BstYI r'GATC_y Cuts at: 1972 2736 2916 3763 4130 4515 4532 5984 6070 Size: 764 180 847 367 385 17 1452 86 Cuts at: 6070 6082 6428 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 847 764 385 367 346 180 86 17 12 BstZ17I GTA'TAC Cuts at: 2834 2834 Size: 6433 Bsu36I CC'TnA_GG Cuts at: 2191 2383 2649 2191 Size: 192 266 5975 Fragments arranged by size: 5975 266 192 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4942 Fragments arranged by size: 4942 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5694 Fragments arranged by size: 5694 683 56 BtsI GCAGTG_nn' Cuts at: 2563 2965 3426 3949 4743 4763 2563 Size: 402 461 523 794 20 4233 Fragments arranged by size: 4233 794 523 461 402 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6433 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6398 DraI TTT'AAA Cuts at: 3737 3737 Size: 6433 DraIII CAC_nnn'GTG Cuts at: 3283 3283 Size: 6433 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2636 5440 809 Size: 507 1320 2804 1802 Fragments arranged by size: 2804 1802 1320 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3723 3880 4769 Size: 54 1425 50 275 18 1893 157 889 Cuts at: 4769 6398 8 Size: 1629 43 Fragments arranged by size: 1893 1629 1425 889 275 157 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3880 1537 Size: 275 2068 4090 Fragments arranged by size: 4090 2068 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2329 2460 4363 1353 Size: 976 131 1903 3423 Fragments arranged by size: 3423 1903 976 131 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2550 2873 3056 3677 5020 5394 Size: 1064 138 961 323 183 621 1343 374 Cuts at: 5394 5540 387 Size: 146 1280 Fragments arranged by size: 1343 1280 1064 961 621 374 323 183 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2328 2885 3068 3368 4496 5093 5880 Size: 393 696 557 183 300 1128 597 787 Cuts at: 5880 6148 1239 Size: 268 1524 Fragments arranged by size: 1524 1128 787 696 597 557 393 300 268 183 EcoNI CCTnn'n_nnAGG Cuts at: 2522 2522 Size: 6433 EcoRI G'AATT_C Cuts at: 3742 4120 3742 Size: 378 6055 FspI TGC'GCA Cuts at: 3362 4939 3362 Size: 1577 4856 HaeII r_GCGC'y Cuts at: 1678 5580 1678 Size: 3902 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2089 2121 2198 2204 2230 Size: 32 135 32 517 32 77 6 26 Cuts at: 2230 2236 3236 3268 5074 5106 5148 5180 1373 Size: 6 1000 32 1806 32 42 32 2626 Fragments arranged by size: 2626 1806 1000 517 135 77 42 32 32 32 32 32 32 26 6 6 HincII GTy'rAC Cuts at: 669 1657 1938 3777 4023 669 Size: 988 281 1839 246 3079 Fragments arranged by size: 3079 1839 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6433 HpaI GTT'AAC Cuts at: 4023 4023 Size: 6433 KpnI G_GTAC'C Cuts at: 3758 3758 Size: 6433 MfeI C'AATT_G Cuts at: 4032 4032 Size: 6433 MluI A'CGCG_T Cuts at: 6309 6309 Size: 6433 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2706 5547 5731 1305 Size: 1401 2841 184 2007 Fragments arranged by size: 2841 2007 1401 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3725 10 Size: 54 1425 343 1893 2718 Fragments arranged by size: 2718 1893 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2201 3108 3124 3628 3710 4391 4750 Size: 648 1035 907 16 504 82 681 359 Cuts at: 4750 4909 518 Size: 159 2042 Fragments arranged by size: 2042 1035 907 681 648 504 359 159 82 16 MspA1I CmG'CkG Cuts at: 1606 2110 2230 3413 3849 4526 5674 5919 1606 Size: 504 120 1183 436 677 1148 245 2120 Fragments arranged by size: 2120 1183 1148 677 504 436 245 120 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4942 Fragments arranged by size: 4942 939 552 NdeI CA'TA_TG Cuts at: 387 3357 387 Size: 2970 3463 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6433 NotI GC'GGCC_GC Cuts at: 3880 3880 Size: 6433 NspI r_CATG'y Cuts at: 1132 1229 3293 3791 5336 1132 Size: 97 2064 498 1545 2229 Fragments arranged by size: 2229 2064 1545 498 97 PasI CC'CwG_GG Cuts at: 1348 3586 1348 Size: 2238 4195 PciI A'CATG_T Cuts at: 5332 5332 Size: 6433 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6433 PmeI GTTT'AAAC Cuts at: 3737 3737 Size: 6433 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4502 4534 6015 6047 4502 Size: 32 1481 32 4888 Fragments arranged by size: 4888 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6433 PsiI TTA'TAA Cuts at: 4003 4003 Size: 6433 PstI C_TGCA'G Cuts at: 830 2481 3177 3320 3785 830 Size: 1651 696 143 465 3478 Fragments arranged by size: 3478 1651 696 465 143 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6433 PvuII CAG'CTG Cuts at: 2110 2230 2110 Size: 120 6313 SalI G'TCGA_C Cuts at: 1936 3775 1936 Size: 1839 4594 SapI GCTCTTCn'nnn_ Cuts at: 2460 2460 Size: 6433 SbfI CC_TGCA'GG Cuts at: 2481 3785 2481 Size: 1304 5129 ScaI AGT'ACT Cuts at: 4681 6393 4681 Size: 1712 4721 SfcI C'TryA_G Cuts at: 826 1045 2289 2477 2686 2854 3173 3316 3781 Size: 219 1244 188 209 168 319 143 465 Cuts at: 3781 4916 5597 5788 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1244 1135 681 465 319 219 209 191 188 168 143 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6433 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6433 SmaI CCC'GGG Cuts at: 1872 2579 3760 1872 Size: 707 1181 4545 Fragments arranged by size: 4545 1181 707 SmlI C'TyrA_G Cuts at: 820 1017 1943 3438 3444 3468 4537 5438 5700 Size: 197 926 1495 6 24 1069 901 262 Cuts at: 5700 820 Size: 1553 Fragments arranged by size: 1553 1495 1069 926 901 262 197 24 6 SnaBI TAC'GTA Cuts at: 493 4224 493 Size: 3731 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6433 SphI G_CATG'C Cuts at: 1132 3293 3791 1132 Size: 2161 498 3774 Fragments arranged by size: 3774 2161 498 SspI AAT'ATT Cuts at: 5 52 4357 5 Size: 47 4305 2081 Fragments arranged by size: 4305 2081 47 StuI AGG'CCT Cuts at: 1517 3062 3491 1517 Size: 1545 429 4459 Fragments arranged by size: 4459 1545 429 StyI C'CwwG_G Cuts at: 513 1065 2004 3294 3872 513 Size: 552 939 1290 578 3074 Fragments arranged by size: 3074 1290 939 578 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3150 4460 4477 4630 4815 5260 325 Size: 1255 1570 1310 17 153 185 445 1498 Fragments arranged by size: 1570 1498 1310 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3300 4679 6391 Size: 274 80 33 51 1443 1323 1379 1712 Cuts at: 6391 96 Size: 138 Fragments arranged by size: 1712 1443 1379 1323 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3546 3578 6393 6425 3546 Size: 32 2815 32 3554 Fragments arranged by size: 3554 2815 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6433 XbaI T'CTAG_A Cuts at: 3769 3769 Size: 6433 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3593 3593 Size: 6433 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6433 XmaI C'CCGG_G Cuts at: 1870 2577 3758 1870 Size: 707 1181 4545 Fragments arranged by size: 4545 1181 707 XmnI GAAnn'nnTTC Cuts at: 2879 3062 3152 4562 2879 Size: 183 90 1410 4750 Fragments arranged by size: 4750 1410 183 90 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4241 276 Size: 53 83 186 936 2707 2468 Fragments arranged by size: 2707 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BsmBI EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI